CN114455999A - Special microbial agent for newly-repaired terrace soil in loess hilly area and preparation method thereof - Google Patents
Special microbial agent for newly-repaired terrace soil in loess hilly area and preparation method thereof Download PDFInfo
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- CN114455999A CN114455999A CN202210111866.4A CN202210111866A CN114455999A CN 114455999 A CN114455999 A CN 114455999A CN 202210111866 A CN202210111866 A CN 202210111866A CN 114455999 A CN114455999 A CN 114455999A
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- 239000002689 soil Substances 0.000 title claims abstract description 63
- 230000000813 microbial effect Effects 0.000 title claims abstract description 49
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000010902 straw Substances 0.000 claims abstract description 27
- 239000011573 trace mineral Substances 0.000 claims abstract description 22
- 150000001875 compounds Chemical class 0.000 claims abstract description 21
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 20
- 235000010958 polyglycerol polyricinoleate Nutrition 0.000 claims abstract description 20
- 239000011591 potassium Substances 0.000 claims abstract description 20
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 20
- 239000002699 waste material Substances 0.000 claims abstract description 19
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 16
- 239000002028 Biomass Substances 0.000 claims abstract description 14
- 239000000783 alginic acid Substances 0.000 claims abstract description 14
- 235000010443 alginic acid Nutrition 0.000 claims abstract description 14
- 229960001126 alginic acid Drugs 0.000 claims abstract description 14
- 229920000615 alginic acid Polymers 0.000 claims abstract description 14
- 150000004781 alginic acids Chemical class 0.000 claims abstract description 14
- 239000003610 charcoal Substances 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 14
- 239000002131 composite material Substances 0.000 claims abstract description 11
- 230000004151 fermentation Effects 0.000 claims description 34
- 238000000855 fermentation Methods 0.000 claims description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 15
- 238000001035 drying Methods 0.000 claims description 12
- 235000011430 Malus pumila Nutrition 0.000 claims description 11
- 244000070406 Malus silvestris Species 0.000 claims description 11
- 235000015103 Malus silvestris Nutrition 0.000 claims description 11
- 238000010563 solid-state fermentation Methods 0.000 claims description 11
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 239000002609 medium Substances 0.000 claims description 10
- 239000002068 microbial inoculum Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- 239000011550 stock solution Substances 0.000 claims description 6
- 241000186361 Actinobacteria <class> Species 0.000 claims description 5
- 241000589152 Azotobacter chroococcum Species 0.000 claims description 5
- 241000194108 Bacillus licheniformis Species 0.000 claims description 5
- 244000063299 Bacillus subtilis Species 0.000 claims description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 5
- 241000194105 Paenibacillus polymyxa Species 0.000 claims description 5
- 244000018633 Prunus armeniaca Species 0.000 claims description 5
- 235000009827 Prunus armeniaca Nutrition 0.000 claims description 5
- 241000223259 Trichoderma Species 0.000 claims description 5
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 5
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 5
- 239000011609 ammonium molybdate Substances 0.000 claims description 5
- 229940010552 ammonium molybdate Drugs 0.000 claims description 5
- 229910021538 borax Inorganic materials 0.000 claims description 5
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 5
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 5
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 5
- 239000011790 ferrous sulphate Substances 0.000 claims description 5
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 5
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 5
- 239000004328 sodium tetraborate Substances 0.000 claims description 5
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 5
- 229960001763 zinc sulfate Drugs 0.000 claims description 5
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 5
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 3
- 241000194107 Bacillus megaterium Species 0.000 claims description 3
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 3
- 241000881860 Paenibacillus mucilaginosus Species 0.000 claims description 3
- 241000589540 Pseudomonas fluorescens Species 0.000 claims description 3
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 3
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 3
- 240000006365 Vitis vinifera Species 0.000 claims description 3
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 3
- 239000007633 bacillus mucilaginosus Substances 0.000 claims description 3
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 3
- 240000004658 Medicago sativa Species 0.000 claims description 2
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims description 2
- 235000017784 Mespilus germanica Nutrition 0.000 claims description 2
- 244000182216 Mimusops elengi Species 0.000 claims description 2
- 235000000560 Mimusops elengi Nutrition 0.000 claims description 2
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 2
- 244000061456 Solanum tuberosum Species 0.000 claims description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 2
- 235000007837 Vangueria infausta Nutrition 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 241001061906 Caragana Species 0.000 claims 1
- 235000015533 Prunus davidiana Nutrition 0.000 claims 1
- 240000002381 Prunus davidiana Species 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 abstract description 7
- 239000003337 fertilizer Substances 0.000 abstract description 6
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 241001631271 Prunus fasciculata Species 0.000 description 4
- 229910052796 boron Inorganic materials 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 229940010514 ammonium ferrous sulfate Drugs 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 229910001385 heavy metal Inorganic materials 0.000 description 3
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 3
- 230000035699 permeability Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052750 molybdenum Inorganic materials 0.000 description 2
- 239000011733 molybdenum Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- -1 0.2 Chemical compound 0.000 description 1
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 241000223025 Caragana microphylla Species 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 239000003617 indole-3-acetic acid Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 238000009991 scouring Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/40—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Pest Control & Pesticides (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Mechanical Engineering (AREA)
- Botany (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a special microbial agent for newly-built terrace soil in loess hilly areas and a preparation method thereof, and relates to the technical field of bacterial fertilizers. The raw materials comprise the following components in parts by weight: 5-15 parts of PGPR composite microbial flora, 25-35 parts of crop straws, 25-35 parts of waste branches, 6-10 parts of biomass charcoal, 5-8 parts of medium and trace element compound, 5-8 parts of potassium fulvate and 4-6 parts of alginic acid. The raw materials of the invention are simple and easy to obtain, the cost is low, the characteristics of different raw materials are reasonably matched and coordinated with each other, and the conditions of insufficient soil nutrients, poor physicochemical characteristics and poor structure of terraced fields newly built in loess hilly areas and unsuitability for planting are obviously improved.
Description
Technical Field
The invention relates to the technical field of bacterial fertilizers, in particular to a special microbial agent for newly-built terrace soil in loess hilly areas and a preparation method thereof.
Background
Loess hills (loess hills) are the main loess landscape on loess plateau. The loess plateau land vegetation and an ecological system are seriously damaged due to loose loess, and the loess plateau land rain season is concentrated in seventy and august months, has high rainfall intensity and is formed by surface water scouring. Loess hills can be divided into two types according to their forms; the long strips are called as beams, and the oval or round strips are called as loess hills. The top areas of the ridges and the loess hills are not large, but the area occupied by the slopes is large, and the slope is generally 10-35 degrees. The loess hills and the grounds in the loess hills are very broken, and the furrows have high density. In the loess hilly area, water and soil loss is serious, so terraces need to be built, trees need to be planted, and grass needs to be planted to maintain water and soil.
The newly-built terrace has the problems that most of the mature soil layer in the original cultivation is moved to the soil-filling part, so that the raw soil is exposed, the moisture, the nutrient and the structure of the soil are changed, the soil cutting part and the soil-filling part of the terrace have great difference in water, fertilizer, gas, heat and the like, and the soil structure is damaged by mechanical disturbance, so that the surface soil is deeply buried, the raw soil is exposed, the soil layer is hard, and the terrace is not suitable for planting trees and grass.
Therefore, the special microbial agent for the soil of the new terraced fields in the loess hilly areas is provided, the conditions of insufficient nutrients, poor physicochemical properties and poor structure of the new terraced fields in the loess hilly areas are improved, and the special microbial agent has important significance for the new terraced fields in the loess hilly areas.
Disclosure of Invention
The invention aims to provide a special microbial agent for soil of a new terrace in a loess hilly area, which solves the problems in the prior art, improves the conditions of insufficient nutrients, poor physicochemical properties and poor structure of the new terrace in the loess hilly area, is suitable for planting and promotes the growth of plants.
In order to achieve the purpose, the invention provides the following scheme:
according to one technical scheme of the invention, the special microbial agent for the newly-built terrace soil in the loess hilly area comprises the following raw materials in parts by weight: 5-15 parts of PGPR composite microbial flora, 25-35 parts of crop straws, 25-35 parts of waste branches, 6-10 parts of biomass charcoal, 5-8 parts of medium and trace element compound, 5-8 parts of potassium fulvate and 4-6 parts of alginic acid.
Further, the PGPR complex microbial flora consists of bacillus subtilis, bacillus polymyxa, trichoderma, actinomycetes, bacillus licheniformis, bacillus megaterium, bacillus thuringiensis, pseudomonas fluorescens, bacillus amyloliquefaciens, bacillus mucilaginosus and azotobacter chroococcum.
Further, the mass ratio of the bacillus subtilis, the bacillus polymyxa, the trichoderma, the actinomycetes, the bacillus licheniformis and the azotobacter chroococcum is 1:1:1:1: 1.
Further, the crop straw is one or more of alfalfa, potato, bean stem straw and gramineous crop straw.
Further, the waste branches are one or more of medlar branches, grape branches, caragana microphylla branches, apple branches, wild apricot branches and wild peach branches.
Further, the medium-trace element compound comprises the following raw materials in parts by weight: 5-10 parts of calcium superphosphate, 5-10 parts of magnesium sulfate, 5-10 parts of zinc sulfate, 10-15 parts of borax, 0.1-0.5 part of ammonium molybdate and 1-5 parts of ferrous sulfate.
The second technical scheme of the invention is that the preparation method of the special microbial agent for the terraced field soil newly-built in the loess hilly areas comprises the following steps:
crushing crop straws and waste branches, drying, adding an EM stock solution fermentation microbial inoculum, adjusting the water content to about 60%, performing aerobic solid state fermentation, adding potassium fulvate, alginic acid, medium and trace element compound, biomass charcoal and PGPR compound microbial flora, performing secondary fermentation for 5-7 days, granulating, and drying to obtain the special microbial inoculum for the newly-built terrace soil in the loess hilly area.
Further, the pulverization is carried out until the particle size is 80-120 meshes.
Further, the C/N ratio in the aerobic solid state fermentation process is 28:1, and the water content is 58-62%.
Further, the temperature of the aerobic solid state fermentation is 60-65 ℃, the fermentation time is 10-30 days, the material turning is started from the beginning of the fermentation to the time when the temperature of the fermented material reaches 60-65 ℃, and the process is repeated for 3-5 times until the fermentation is finished.
Further, the temperature of the secondary fermentation is 60-65 ℃.
According to the third technical scheme, the special microbial agent for the soil of the new terraced fields in the loess hilly areas is applied to the new terraced fields in the loess hilly areas.
The invention discloses the following technical effects:
(1) the PGPR compound microbial flora in the raw materials can continuously secrete auxin such as cytokinin, indoleacetic acid, gibberellin and the like, amino acid and other active substances, so that rooting is promoted, and the water and fertilizer absorption capacity of a plant root system is enhanced; meanwhile, the PGPR compound microbial flora also has the effects of activating nutrients, prolonging the fertilizer efficiency, loosening the soil and neutralizing the pH value.
(2) After aerobic solid-state fermentation is carried out on the waste straws and the waste branches, organic materials which are loose in texture and rich in organic matter content are formed. The fertilizer has the effects of improving soil hardening, improving soil permeability, increasing surface area and strong adsorption capacity, and can be used for improving soil air permeability, improving soil water-holding capacity and promoting crop root growth.
(3) The medium trace element compound added in the invention is reasonably prepared, can provide trace elements such as boron, calcium, magnesium, zinc, boron, molybdenum, iron and the like necessary for plants, and can effectively prevent physiological diseases caused by the deficiency of the trace elements such as boron, calcium, magnesium, zinc, boron, molybdenum, iron and the like in the plants.
(4) The biomass charcoal is loose and porous, can adsorb pollutants such as heavy metal ions in soil, provides a good growth ecological environment for plants, and simultaneously improves the air permeability and soil properties of the plants.
(5) The potassium fulvate is an organic substance with colloid property, can loosen soil and increase the absorbed water amount, has the functions of ventilation, humidification, entropy maintenance and drought prevention, and improves the water, gas and heat conditions of the soil; it can also increase the activity of enzymes in plants and increase the resistance of plant matrix; the potassium fulvate can perform exchange reaction with ions in the soil to fix heavy metal ions in the soil and prevent the heavy metal ions from entering biological circulation; the potassium fulvate has the functions of fixing nitrogen, dissolving phosphorus and activating potassium, and can also have chelation with insoluble trace elements to generate humic acid trace element chelate which has good solubility and can be absorbed by plants, so that the plant root system can absorb trace elements; the potassium fulvate can stimulate the growth of root systems, so that the plants have better soil fixing capacity and prevent water and soil loss of terraces. The potassium fulvate has more functional groups, can be combined with acid and alkali, and can adjust the pH value of soil according to the needs of crops.
(6) Alginic acid can stimulate the activity of a plant defense enzyme system, enhance the drought and cold resistance of plants, promote the development of plant root systems and improve the absorption and utilization of soil nutrients and water by the plants.
(7) The crop straws and the waste branches used in the invention are all wastes, and the waste treatment and resource utilization are realized. The raw materials of the invention are simple and easy to obtain, the cost is low, the characteristics of different raw materials are reasonably matched and coordinated with each other, and the conditions that the newly-built terrace soil in loess hilly areas is insufficient in nutrients, poor in physicochemical characteristics and structure and not suitable for planting are obviously improved.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
The "parts" in the present invention are in parts by weight unless otherwise specified.
The starting materials used in the present invention are commercially available unless otherwise specified.
The PGPR compound microbial flora is obtained by respectively culturing bacillus subtilis, bacillus polymyxa, trichoderma, actinomycetes, bacillus licheniformis, bacillus megaterium, bacillus thuringiensis, pseudomonas fluorescens, bacillus amyloliquefaciens, bacillus mucilaginosus and azotobacter chroococcum, centrifuging, freeze-drying and mixing the components by mass (the culture methods are all conventional technical means in the field, and are not described herein any more).
Example 1
Accurately weighing the following raw materials: 10 parts of PGPR composite microbial flora, 30 parts of crop straws (soybean straws and corn straws are mixed according to the mass ratio of 1: 1), 30 parts of waste branches (apple branches, apricot branches and wild peach branches are mixed according to the mass ratio of 1:1: 1), 8 parts of biomass charcoal, 6 parts of medium-trace element compound (calcium superphosphate, magnesium sulfate, zinc sulfate, borax, ammonium molybdate and ferrous sulfate are mixed according to the mass ratio of 8:8:8:13:0.3: 3), 6 parts of potassium fulvate and 5 parts of alginic acid.
The preparation method comprises the following steps: crushing the crop straws and the waste branches in parts by weight to 120 meshes, drying at 60 ℃, adding an EM stock solution fermentation microbial inoculum, adjusting the C/N to be 28:1 and the water content to be 58-62%, carrying out aerobic solid state fermentation at 60 ℃ for 20 days, starting to turn over materials from the beginning of fermentation to the temperature of the fermentation materials reaching 60 ℃, and repeating for 4 times until the fermentation is finished; and adding the potassium fulvate, alginic acid, the medium-trace element compound, the biomass charcoal and the PGPR composite microbial flora in parts by weight, performing secondary fermentation for 5d (5-7d has an effect equivalent to 5 d) at 65 ℃ (60-65 ℃ and 65 ℃), granulating, and drying for 3h at 65 ℃ to obtain the special microbial agent for the newly-repaired terrace soil in the loess hilly area.
Example 2
Accurately weighing the following raw materials: 15 parts of PGPR composite microbial flora, 35 parts of crop straws (soybean straws and corn straws are mixed according to the mass ratio of 1: 1), 25 parts of waste branches (apple branches, apricot branches and wild peach branches are mixed according to the mass ratio of 1:1: 1), 10 parts of biomass charcoal, 8 parts of medium and trace element compound (calcium superphosphate, magnesium sulfate, zinc sulfate, borax, ammonium molybdate and ferrous sulfate are mixed according to the mass ratio of 10:10:10:15:0.5: 5), 8 parts of potassium fulvate and 6 parts of alginic acid.
The preparation method comprises the following steps: crushing the crop straws and the waste branches in parts by weight to 100 meshes, drying at 65 ℃, adding an EM stock solution fermentation microbial inoculum, adjusting the C/N to be 28:1 and the water content to be 58-62%, carrying out aerobic solid state fermentation at 65 ℃ for 15 days, starting turning materials from the beginning of fermentation to the time when the temperature of the fermentation materials reaches 60 ℃, and repeating for 3 times until the end of fermentation; and adding the potassium fulvate, the alginic acid, the medium-trace element compound, the biomass charcoal and the PGPR composite microbial flora in parts by weight, performing secondary fermentation for 5 days at 65 ℃, granulating, and drying for 3 hours at 65 ℃ to obtain the special microbial agent for the newly-repaired terrace soil in the loess hilly area.
Example 3
Accurately weighing the following raw materials: 5 parts of PGPR composite microbial flora, 25 parts of crop straw (soybean straw and corn straw are mixed according to the mass ratio of 1: 1), 35 parts of waste branches (apple branches, apricot branches and wild peach branches are mixed according to the mass ratio of 1:1: 1), 6 parts of biomass charcoal, 5 parts of medium and trace element compound (calcium superphosphate, magnesium sulfate, zinc sulfate, borax, ammonium molybdate and ferrous sulfate are mixed according to the mass ratio of 5:5:5:10:0.1: 1), 5 parts of potassium fulvate and 4 parts of alginic acid.
The preparation method comprises the following steps: crushing the crop straws and the waste branches in parts by weight to 120 meshes, drying at 60 ℃, adding an EM stock solution fermentation microbial inoculum, adjusting the C/N to be 28:1 and the water content to be 58-62%, carrying out aerobic solid state fermentation at 60 ℃ for 10 days, starting turning materials from the beginning of fermentation to the time when the temperature of the fermentation materials reaches 60 ℃, and repeating for 3 times until the end of fermentation; and adding the potassium fulvate, the alginic acid, the medium-trace element compound, the biomass charcoal and the PGPR composite microbial flora in parts by weight, performing secondary fermentation for 5 days at 65 ℃, granulating, and drying for 3 hours at 65 ℃ to obtain the special microbial agent for the newly-repaired terrace soil in the loess hilly area.
Example 1
Accurately weighing the following raw materials: 10 parts of PGPR composite microbial flora, 30 parts of wheat straw, 30 parts of grape branches, 8 parts of biomass charcoal, 6 parts of medium and trace element compound (mixed by 8:8:8:13:0.3:3 by mass) 6 parts of potassium fulvate and 5 parts of alginic acid.
The preparation method comprises the following steps: crushing the crop straws and the waste branches in parts by weight to 120 meshes, drying at 60 ℃, adding an EM stock solution fermentation microbial inoculum, adjusting the C/N to be 28:1 and the water content to be 58-62%, carrying out aerobic solid state fermentation at 60 ℃ for 20 days, starting turning materials from the beginning of fermentation to the time when the temperature of the fermentation materials reaches 60 ℃, and repeating for 4 times until the end of fermentation; and adding the potassium fulvate, alginic acid, the medium-trace element compound, the biomass charcoal and the PGPR composite microbial flora in parts by weight, performing secondary fermentation for 5-7 days, granulating, and drying at 65 ℃ for 3 hours to obtain the special microbial agent for the newly-repaired terrace soil in the loess hilly area.
Comparative example 1
The only difference was that the addition of the PGPR complex microorganism colony and potassium fulvate was omitted, as in example 1.
Comparative example 2
The only difference is that the addition of the PGPR complex microbial population is omitted as in example 1.
Comparative example 3
As in example 1, the only difference is that the addition of the medium trace element complex is omitted.
Comparative example 4
The only difference is that the solid state fermentation treatment step is omitted, as in example 1.
Test example 1
The special microbial agents for soil in the new terraced fields in the loess hilly areas, prepared in examples 1 to 4 and comparative examples 1 to 4, were applied to the same divided areas of the new terraced fields in the loess hilly areas, and then apple seedlings were transplanted with soil masses, and the growth conditions and soil conditions of the apple seedlings in each area were observed, and the results are shown in table 1.
TABLE 1
As can be seen from Table 1, the application of the microbial agent special for soil in newly-built terrace in loess hilly areas greatly shortens the seedling reviving time when apple trees are transplanted. By applying the microbial agents in the embodiments 1 to 4, the trunk diameters of the apple seedlings are respectively increased by 2.0cm, 1.9cm, 1.8cm and 1.9cm after the apple seedlings are transplanted for one year, which is obviously superior to the trunk diameters of 1.0cm, 1.5cm, 1.4cm and 1.6cm of the apple seedlings in the comparative examples 1 to 4, and is also superior to the trunk diameter increase speed of 1.0 to 1.5 cm/year which is common in the prior art.
The soil in the loess hilly area is alkaline, and after the microbial agent prepared in the embodiment 1-4 of the invention is applied for one year, the pH value of the soil is obviously reduced, and is respectively reduced by 0.6, 0.4 and 0.5.0.6, which is better than that of the comparative example 1-4, such as 0.2, 0.3 and 0.2. The invention also shows that the special microbial agent for soil of the new terraced fields in the loess hilly areas can obviously improve the alkaline condition of the soil.
The newly-built terrace is superior to the bare raw soil, so that the soil layer is hard, and as can be seen from the table 1, after the microbial agent prepared by the invention is applied, the hard state of the soil is improved, and the growth of plants is facilitated.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (10)
1. The special microbial agent for terraced fields soil newly-built in loess hilly areas is characterized in that the raw materials comprise the following components in parts by weight: 5-15 parts of PGPR composite microbial flora, 25-35 parts of crop straws, 25-35 parts of waste branches, 6-10 parts of biomass charcoal, 5-8 parts of medium and trace element compound, 5-8 parts of potassium fulvate and 4-6 parts of alginic acid.
2. The microbial agent for soil of newly-built terraced fields in loess hilly areas as claimed in claim 1, wherein the PGPR complex microbial flora consists of Bacillus subtilis, Bacillus polymyxa, Trichoderma, Actinomycetes, Bacillus licheniformis, Bacillus megaterium, Bacillus thuringiensis, Pseudomonas fluorescens, Bacillus amyloliquefaciens, Bacillus mucilaginosus and azotobacter chroococcum.
3. The microbial agent special for the soil of the new terrace in the loess hilly area as claimed in claim 2, wherein the mass ratio of bacillus subtilis, bacillus polymyxa, trichoderma, actinomycetes, bacillus licheniformis and azotobacter chroococcum is 1:1:1:1: 1.
4. The microbial agent for soil in terraced fields newly built in loess hilly areas as claimed in claim 1, wherein the crop straw is one or more of alfalfa, potato, bean stalk straw and gramineous crop straw.
5. The microbial agent special for the soil of the newly-built terrace in the loess hilly area according to claim 1, wherein the waste branches are one or more of medlar branches, grape branches, caragana branches, apple branches, prunus armeniaca branches and prunus davidiana branches.
6. The microbial agent special for soil in terraced fields newly built in loess hilly areas as claimed in claim 1, wherein the medium trace element compound comprises the following raw materials in parts by weight: 5-10 parts of calcium superphosphate, 5-10 parts of magnesium sulfate, 5-10 parts of zinc sulfate, 10-15 parts of borax, 0.1-0.5 part of ammonium molybdate and 1-5 parts of ferrous sulfate.
7. The preparation method of the microbial agent special for the loess hilly area newly-built terraced fields soil as claimed in claim 1, which comprises the following steps:
crushing crop straws and waste branches, drying, adding an EM stock solution fermentation microbial inoculum and adjusting the water content, carrying out aerobic solid state fermentation, adding potassium fulvate, alginic acid, a medium trace element compound, biomass charcoal and PGPR compound microbial flora, granulating, and drying to obtain the special microbial inoculum for the newly-repaired terrace soil in the loess hilly area.
8. The method for preparing the microbial agent special for the soil in the new terraced fields in the loess hilly areas as claimed in claim 7, wherein the ratio of C/N in the aerobic solid state fermentation process is 28:1, and the water content is 58-62%.
9. The method for preparing the microbial agent special for the terraced fields soil in the loess hilly areas as claimed in claim 7, wherein the temperature of the aerobic solid fermentation is 60-65 ℃, the fermentation time is 10-30 days, the material turning is started from the beginning of the fermentation to the time when the temperature of the fermentation material reaches 60-65 ℃, and the process is repeated for 3-5 times until the fermentation is finished.
10. The use of the microbial agent for soil-specific terraced fields in loess hilly areas as claimed in any one of claims 1 to 6 in terraced fields in loess hilly areas.
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