CN114455999A - Special microbial agent for newly-repaired terrace soil in loess hilly area and preparation method thereof - Google Patents

Special microbial agent for newly-repaired terrace soil in loess hilly area and preparation method thereof Download PDF

Info

Publication number
CN114455999A
CN114455999A CN202210111866.4A CN202210111866A CN114455999A CN 114455999 A CN114455999 A CN 114455999A CN 202210111866 A CN202210111866 A CN 202210111866A CN 114455999 A CN114455999 A CN 114455999A
Authority
CN
China
Prior art keywords
parts
soil
microbial agent
branches
newly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210111866.4A
Other languages
Chinese (zh)
Inventor
王占军
李凤霞
郭永忠
杜建民
王长军
段晓明
米克进
何建龙
孙娇
沈婧丽
俞鸿千
季波
任小玢
吴旭东
王小军
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agricultural Resource And Environment Institute Ningxia Academy Of Agricultural And Forestry Sciences (ningxia Soil And Plant Nutrition Key Laboratory)
Institute Of Desertification Control Ningxia Academy Of Agriculture And Forestry Sciences (ningxia Key Laboratory Of Sand Control And Soil And Water Conservation)
Original Assignee
Agricultural Resource And Environment Institute Ningxia Academy Of Agricultural And Forestry Sciences (ningxia Soil And Plant Nutrition Key Laboratory)
Institute Of Desertification Control Ningxia Academy Of Agriculture And Forestry Sciences (ningxia Key Laboratory Of Sand Control And Soil And Water Conservation)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agricultural Resource And Environment Institute Ningxia Academy Of Agricultural And Forestry Sciences (ningxia Soil And Plant Nutrition Key Laboratory), Institute Of Desertification Control Ningxia Academy Of Agriculture And Forestry Sciences (ningxia Key Laboratory Of Sand Control And Soil And Water Conservation) filed Critical Agricultural Resource And Environment Institute Ningxia Academy Of Agricultural And Forestry Sciences (ningxia Soil And Plant Nutrition Key Laboratory)
Priority to CN202210111866.4A priority Critical patent/CN114455999A/en
Publication of CN114455999A publication Critical patent/CN114455999A/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/40Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/10Solid or semi-solid fertilisers, e.g. powders
    • C05G5/12Granules or flakes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Pest Control & Pesticides (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a special microbial agent for newly-built terrace soil in loess hilly areas and a preparation method thereof, and relates to the technical field of bacterial fertilizers. The raw materials comprise the following components in parts by weight: 5-15 parts of PGPR composite microbial flora, 25-35 parts of crop straws, 25-35 parts of waste branches, 6-10 parts of biomass charcoal, 5-8 parts of medium and trace element compound, 5-8 parts of potassium fulvate and 4-6 parts of alginic acid. The raw materials of the invention are simple and easy to obtain, the cost is low, the characteristics of different raw materials are reasonably matched and coordinated with each other, and the conditions of insufficient soil nutrients, poor physicochemical characteristics and poor structure of terraced fields newly built in loess hilly areas and unsuitability for planting are obviously improved.

Description

Microbial agent special for terraced soil newly-repaired in loess hilly areas and preparation method thereof
Technical Field
The invention relates to the technical field of bacterial fertilizers, in particular to a special microbial agent for newly-built terrace soil in loess hilly areas and a preparation method thereof.
Background
Loess hills (loess hills) are the main loess landscape on loess plateau. The loess plateau land vegetation and an ecological system are seriously damaged due to loose loess, and the loess plateau land rain season is concentrated in seventy and august months, has high rainfall intensity and is formed by surface water scouring. Loess hills can be divided into two types according to their forms; the long strips are called as beams, and the oval or round strips are called as loess hills. The top areas of the ridges and the loess hills are not large, but the area occupied by the slopes is large, and the slope is generally 10-35 degrees. The loess hills and the grounds in the loess hills are very broken, and the furrows have high density. In the loess hilly area, water and soil loss is serious, so terraces need to be built, trees need to be planted, and grass needs to be planted to maintain water and soil.
The newly-built terrace has the problems that most of the mature soil layer in the original cultivation is moved to the soil-filling part, so that the raw soil is exposed, the moisture, the nutrient and the structure of the soil are changed, the soil cutting part and the soil-filling part of the terrace have great difference in water, fertilizer, gas, heat and the like, and the soil structure is damaged by mechanical disturbance, so that the surface soil is deeply buried, the raw soil is exposed, the soil layer is hard, and the terrace is not suitable for planting trees and grass.
Therefore, the special microbial agent for the soil of the new terraced fields in the loess hilly areas is provided, the conditions of insufficient nutrients, poor physicochemical properties and poor structure of the new terraced fields in the loess hilly areas are improved, and the special microbial agent has important significance for the new terraced fields in the loess hilly areas.
Disclosure of Invention
The invention aims to provide a special microbial agent for soil of a new terrace in a loess hilly area, which solves the problems in the prior art, improves the conditions of insufficient nutrients, poor physicochemical properties and poor structure of the new terrace in the loess hilly area, is suitable for planting and promotes the growth of plants.
In order to achieve the purpose, the invention provides the following scheme:
according to one technical scheme of the invention, the special microbial agent for the newly-built terrace soil in the loess hilly area comprises the following raw materials in parts by weight: 5-15 parts of PGPR composite microbial flora, 25-35 parts of crop straws, 25-35 parts of waste branches, 6-10 parts of biomass charcoal, 5-8 parts of medium and trace element compound, 5-8 parts of potassium fulvate and 4-6 parts of alginic acid.
Further, the PGPR complex microbial flora consists of bacillus subtilis, bacillus polymyxa, trichoderma, actinomycetes, bacillus licheniformis, bacillus megaterium, bacillus thuringiensis, pseudomonas fluorescens, bacillus amyloliquefaciens, bacillus mucilaginosus and azotobacter chroococcum.
Further, the mass ratio of the bacillus subtilis, the bacillus polymyxa, the trichoderma, the actinomycetes, the bacillus licheniformis and the azotobacter chroococcum is 1:1:1:1: 1.
Further, the crop straw is one or more of alfalfa, potato, bean stem straw and gramineous crop straw.
Further, the waste branches are one or more of medlar branches, grape branches, caragana microphylla branches, apple branches, wild apricot branches and wild peach branches.
Further, the medium-trace element compound comprises the following raw materials in parts by weight: 5-10 parts of calcium superphosphate, 5-10 parts of magnesium sulfate, 5-10 parts of zinc sulfate, 10-15 parts of borax, 0.1-0.5 part of ammonium molybdate and 1-5 parts of ferrous sulfate.
The second technical scheme of the invention is that the preparation method of the special microbial agent for the terraced field soil newly-built in the loess hilly areas comprises the following steps:
crushing crop straws and waste branches, drying, adding an EM stock solution fermentation microbial inoculum, adjusting the water content to about 60%, performing aerobic solid state fermentation, adding potassium fulvate, alginic acid, medium and trace element compound, biomass charcoal and PGPR compound microbial flora, performing secondary fermentation for 5-7 days, granulating, and drying to obtain the special microbial inoculum for the newly-built terrace soil in the loess hilly area.
Further, the pulverization is carried out until the particle size is 80-120 meshes.
Further, the C/N ratio in the aerobic solid state fermentation process is 28:1, and the water content is 58-62%.
Further, the temperature of the aerobic solid state fermentation is 60-65 ℃, the fermentation time is 10-30 days, the material turning is started from the beginning of the fermentation to the time when the temperature of the fermented material reaches 60-65 ℃, and the process is repeated for 3-5 times until the fermentation is finished.
Further, the temperature of the secondary fermentation is 60-65 ℃.
According to the third technical scheme, the special microbial agent for the soil of the new terraced fields in the loess hilly areas is applied to the new terraced fields in the loess hilly areas.
The invention discloses the following technical effects:
(1) the PGPR compound microbial flora in the raw materials can continuously secrete auxin such as cytokinin, indoleacetic acid, gibberellin and the like, amino acid and other active substances, so that rooting is promoted, and the water and fertilizer absorption capacity of a plant root system is enhanced; meanwhile, the PGPR compound microbial flora also has the effects of activating nutrients, prolonging the fertilizer efficiency, loosening the soil and neutralizing the pH value.
(2) After aerobic solid-state fermentation is carried out on the waste straws and the waste branches, organic materials which are loose in texture and rich in organic matter content are formed. The fertilizer has the effects of improving soil hardening, improving soil permeability, increasing surface area and strong adsorption capacity, and can be used for improving soil air permeability, improving soil water-holding capacity and promoting crop root growth.
(3) The medium trace element compound added in the invention is reasonably prepared, can provide trace elements such as boron, calcium, magnesium, zinc, boron, molybdenum, iron and the like necessary for plants, and can effectively prevent physiological diseases caused by the deficiency of the trace elements such as boron, calcium, magnesium, zinc, boron, molybdenum, iron and the like in the plants.
(4) The biomass charcoal is loose and porous, can adsorb pollutants such as heavy metal ions in soil, provides a good growth ecological environment for plants, and simultaneously improves the air permeability and soil properties of the plants.
(5) The potassium fulvate is an organic substance with colloid property, can loosen soil and increase the absorbed water amount, has the functions of ventilation, humidification, entropy maintenance and drought prevention, and improves the water, gas and heat conditions of the soil; it can also increase the activity of enzymes in plants and increase the resistance of plant matrix; the potassium fulvate can perform exchange reaction with ions in the soil to fix heavy metal ions in the soil and prevent the heavy metal ions from entering biological circulation; the potassium fulvate has the functions of fixing nitrogen, dissolving phosphorus and activating potassium, and can also have chelation with insoluble trace elements to generate humic acid trace element chelate which has good solubility and can be absorbed by plants, so that the plant root system can absorb trace elements; the potassium fulvate can stimulate the growth of root systems, so that the plants have better soil fixing capacity and prevent water and soil loss of terraces. The potassium fulvate has more functional groups, can be combined with acid and alkali, and can adjust the pH value of soil according to the needs of crops.
(6) Alginic acid can stimulate the activity of a plant defense enzyme system, enhance the drought and cold resistance of plants, promote the development of plant root systems and improve the absorption and utilization of soil nutrients and water by the plants.
(7) The crop straws and the waste branches used in the invention are all wastes, and the waste treatment and resource utilization are realized. The raw materials of the invention are simple and easy to obtain, the cost is low, the characteristics of different raw materials are reasonably matched and coordinated with each other, and the conditions that the newly-built terrace soil in loess hilly areas is insufficient in nutrients, poor in physicochemical characteristics and structure and not suitable for planting are obviously improved.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
The "parts" in the present invention are in parts by weight unless otherwise specified.
The starting materials used in the present invention are commercially available unless otherwise specified.
The PGPR compound microbial flora is obtained by respectively culturing bacillus subtilis, bacillus polymyxa, trichoderma, actinomycetes, bacillus licheniformis, bacillus megaterium, bacillus thuringiensis, pseudomonas fluorescens, bacillus amyloliquefaciens, bacillus mucilaginosus and azotobacter chroococcum, centrifuging, freeze-drying and mixing the components by mass (the culture methods are all conventional technical means in the field, and are not described herein any more).
Example 1
Accurately weighing the following raw materials: 10 parts of PGPR composite microbial flora, 30 parts of crop straws (soybean straws and corn straws are mixed according to the mass ratio of 1: 1), 30 parts of waste branches (apple branches, apricot branches and wild peach branches are mixed according to the mass ratio of 1:1: 1), 8 parts of biomass charcoal, 6 parts of medium-trace element compound (calcium superphosphate, magnesium sulfate, zinc sulfate, borax, ammonium molybdate and ferrous sulfate are mixed according to the mass ratio of 8:8:8:13:0.3: 3), 6 parts of potassium fulvate and 5 parts of alginic acid.
The preparation method comprises the following steps: crushing the crop straws and the waste branches in parts by weight to 120 meshes, drying at 60 ℃, adding an EM stock solution fermentation microbial inoculum, adjusting the C/N to be 28:1 and the water content to be 58-62%, carrying out aerobic solid state fermentation at 60 ℃ for 20 days, starting to turn over materials from the beginning of fermentation to the temperature of the fermentation materials reaching 60 ℃, and repeating for 4 times until the fermentation is finished; and adding the potassium fulvate, alginic acid, the medium-trace element compound, the biomass charcoal and the PGPR composite microbial flora in parts by weight, performing secondary fermentation for 5d (5-7d has an effect equivalent to 5 d) at 65 ℃ (60-65 ℃ and 65 ℃), granulating, and drying for 3h at 65 ℃ to obtain the special microbial agent for the newly-repaired terrace soil in the loess hilly area.
Example 2
Accurately weighing the following raw materials: 15 parts of PGPR composite microbial flora, 35 parts of crop straws (soybean straws and corn straws are mixed according to the mass ratio of 1: 1), 25 parts of waste branches (apple branches, apricot branches and wild peach branches are mixed according to the mass ratio of 1:1: 1), 10 parts of biomass charcoal, 8 parts of medium and trace element compound (calcium superphosphate, magnesium sulfate, zinc sulfate, borax, ammonium molybdate and ferrous sulfate are mixed according to the mass ratio of 10:10:10:15:0.5: 5), 8 parts of potassium fulvate and 6 parts of alginic acid.
The preparation method comprises the following steps: crushing the crop straws and the waste branches in parts by weight to 100 meshes, drying at 65 ℃, adding an EM stock solution fermentation microbial inoculum, adjusting the C/N to be 28:1 and the water content to be 58-62%, carrying out aerobic solid state fermentation at 65 ℃ for 15 days, starting turning materials from the beginning of fermentation to the time when the temperature of the fermentation materials reaches 60 ℃, and repeating for 3 times until the end of fermentation; and adding the potassium fulvate, the alginic acid, the medium-trace element compound, the biomass charcoal and the PGPR composite microbial flora in parts by weight, performing secondary fermentation for 5 days at 65 ℃, granulating, and drying for 3 hours at 65 ℃ to obtain the special microbial agent for the newly-repaired terrace soil in the loess hilly area.
Example 3
Accurately weighing the following raw materials: 5 parts of PGPR composite microbial flora, 25 parts of crop straw (soybean straw and corn straw are mixed according to the mass ratio of 1: 1), 35 parts of waste branches (apple branches, apricot branches and wild peach branches are mixed according to the mass ratio of 1:1: 1), 6 parts of biomass charcoal, 5 parts of medium and trace element compound (calcium superphosphate, magnesium sulfate, zinc sulfate, borax, ammonium molybdate and ferrous sulfate are mixed according to the mass ratio of 5:5:5:10:0.1: 1), 5 parts of potassium fulvate and 4 parts of alginic acid.
The preparation method comprises the following steps: crushing the crop straws and the waste branches in parts by weight to 120 meshes, drying at 60 ℃, adding an EM stock solution fermentation microbial inoculum, adjusting the C/N to be 28:1 and the water content to be 58-62%, carrying out aerobic solid state fermentation at 60 ℃ for 10 days, starting turning materials from the beginning of fermentation to the time when the temperature of the fermentation materials reaches 60 ℃, and repeating for 3 times until the end of fermentation; and adding the potassium fulvate, the alginic acid, the medium-trace element compound, the biomass charcoal and the PGPR composite microbial flora in parts by weight, performing secondary fermentation for 5 days at 65 ℃, granulating, and drying for 3 hours at 65 ℃ to obtain the special microbial agent for the newly-repaired terrace soil in the loess hilly area.
Example 1
Accurately weighing the following raw materials: 10 parts of PGPR composite microbial flora, 30 parts of wheat straw, 30 parts of grape branches, 8 parts of biomass charcoal, 6 parts of medium and trace element compound (mixed by 8:8:8:13:0.3:3 by mass) 6 parts of potassium fulvate and 5 parts of alginic acid.
The preparation method comprises the following steps: crushing the crop straws and the waste branches in parts by weight to 120 meshes, drying at 60 ℃, adding an EM stock solution fermentation microbial inoculum, adjusting the C/N to be 28:1 and the water content to be 58-62%, carrying out aerobic solid state fermentation at 60 ℃ for 20 days, starting turning materials from the beginning of fermentation to the time when the temperature of the fermentation materials reaches 60 ℃, and repeating for 4 times until the end of fermentation; and adding the potassium fulvate, alginic acid, the medium-trace element compound, the biomass charcoal and the PGPR composite microbial flora in parts by weight, performing secondary fermentation for 5-7 days, granulating, and drying at 65 ℃ for 3 hours to obtain the special microbial agent for the newly-repaired terrace soil in the loess hilly area.
Comparative example 1
The only difference was that the addition of the PGPR complex microorganism colony and potassium fulvate was omitted, as in example 1.
Comparative example 2
The only difference is that the addition of the PGPR complex microbial population is omitted as in example 1.
Comparative example 3
As in example 1, the only difference is that the addition of the medium trace element complex is omitted.
Comparative example 4
The only difference is that the solid state fermentation treatment step is omitted, as in example 1.
Test example 1
The special microbial agents for soil in the new terraced fields in the loess hilly areas, prepared in examples 1 to 4 and comparative examples 1 to 4, were applied to the same divided areas of the new terraced fields in the loess hilly areas, and then apple seedlings were transplanted with soil masses, and the growth conditions and soil conditions of the apple seedlings in each area were observed, and the results are shown in table 1.
TABLE 1
Figure BDA0003491541390000091
As can be seen from Table 1, the application of the microbial agent special for soil in newly-built terrace in loess hilly areas greatly shortens the seedling reviving time when apple trees are transplanted. By applying the microbial agents in the embodiments 1 to 4, the trunk diameters of the apple seedlings are respectively increased by 2.0cm, 1.9cm, 1.8cm and 1.9cm after the apple seedlings are transplanted for one year, which is obviously superior to the trunk diameters of 1.0cm, 1.5cm, 1.4cm and 1.6cm of the apple seedlings in the comparative examples 1 to 4, and is also superior to the trunk diameter increase speed of 1.0 to 1.5 cm/year which is common in the prior art.
The soil in the loess hilly area is alkaline, and after the microbial agent prepared in the embodiment 1-4 of the invention is applied for one year, the pH value of the soil is obviously reduced, and is respectively reduced by 0.6, 0.4 and 0.5.0.6, which is better than that of the comparative example 1-4, such as 0.2, 0.3 and 0.2. The invention also shows that the special microbial agent for soil of the new terraced fields in the loess hilly areas can obviously improve the alkaline condition of the soil.
The newly-built terrace is superior to the bare raw soil, so that the soil layer is hard, and as can be seen from the table 1, after the microbial agent prepared by the invention is applied, the hard state of the soil is improved, and the growth of plants is facilitated.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims (10)

1. The special microbial agent for terraced fields soil newly-built in loess hilly areas is characterized in that the raw materials comprise the following components in parts by weight: 5-15 parts of PGPR composite microbial flora, 25-35 parts of crop straws, 25-35 parts of waste branches, 6-10 parts of biomass charcoal, 5-8 parts of medium and trace element compound, 5-8 parts of potassium fulvate and 4-6 parts of alginic acid.
2. The microbial agent for soil of newly-built terraced fields in loess hilly areas as claimed in claim 1, wherein the PGPR complex microbial flora consists of Bacillus subtilis, Bacillus polymyxa, Trichoderma, Actinomycetes, Bacillus licheniformis, Bacillus megaterium, Bacillus thuringiensis, Pseudomonas fluorescens, Bacillus amyloliquefaciens, Bacillus mucilaginosus and azotobacter chroococcum.
3. The microbial agent special for the soil of the new terrace in the loess hilly area as claimed in claim 2, wherein the mass ratio of bacillus subtilis, bacillus polymyxa, trichoderma, actinomycetes, bacillus licheniformis and azotobacter chroococcum is 1:1:1:1: 1.
4. The microbial agent for soil in terraced fields newly built in loess hilly areas as claimed in claim 1, wherein the crop straw is one or more of alfalfa, potato, bean stalk straw and gramineous crop straw.
5. The microbial agent special for the soil of the newly-built terrace in the loess hilly area according to claim 1, wherein the waste branches are one or more of medlar branches, grape branches, caragana branches, apple branches, prunus armeniaca branches and prunus davidiana branches.
6. The microbial agent special for soil in terraced fields newly built in loess hilly areas as claimed in claim 1, wherein the medium trace element compound comprises the following raw materials in parts by weight: 5-10 parts of calcium superphosphate, 5-10 parts of magnesium sulfate, 5-10 parts of zinc sulfate, 10-15 parts of borax, 0.1-0.5 part of ammonium molybdate and 1-5 parts of ferrous sulfate.
7. The preparation method of the microbial agent special for the loess hilly area newly-built terraced fields soil as claimed in claim 1, which comprises the following steps:
crushing crop straws and waste branches, drying, adding an EM stock solution fermentation microbial inoculum and adjusting the water content, carrying out aerobic solid state fermentation, adding potassium fulvate, alginic acid, a medium trace element compound, biomass charcoal and PGPR compound microbial flora, granulating, and drying to obtain the special microbial inoculum for the newly-repaired terrace soil in the loess hilly area.
8. The method for preparing the microbial agent special for the soil in the new terraced fields in the loess hilly areas as claimed in claim 7, wherein the ratio of C/N in the aerobic solid state fermentation process is 28:1, and the water content is 58-62%.
9. The method for preparing the microbial agent special for the terraced fields soil in the loess hilly areas as claimed in claim 7, wherein the temperature of the aerobic solid fermentation is 60-65 ℃, the fermentation time is 10-30 days, the material turning is started from the beginning of the fermentation to the time when the temperature of the fermentation material reaches 60-65 ℃, and the process is repeated for 3-5 times until the fermentation is finished.
10. The use of the microbial agent for soil-specific terraced fields in loess hilly areas as claimed in any one of claims 1 to 6 in terraced fields in loess hilly areas.
CN202210111866.4A 2022-01-27 2022-01-27 Special microbial agent for newly-repaired terrace soil in loess hilly area and preparation method thereof Pending CN114455999A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210111866.4A CN114455999A (en) 2022-01-27 2022-01-27 Special microbial agent for newly-repaired terrace soil in loess hilly area and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210111866.4A CN114455999A (en) 2022-01-27 2022-01-27 Special microbial agent for newly-repaired terrace soil in loess hilly area and preparation method thereof

Publications (1)

Publication Number Publication Date
CN114455999A true CN114455999A (en) 2022-05-10

Family

ID=81411515

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210111866.4A Pending CN114455999A (en) 2022-01-27 2022-01-27 Special microbial agent for newly-repaired terrace soil in loess hilly area and preparation method thereof

Country Status (1)

Country Link
CN (1) CN114455999A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115491205A (en) * 2022-08-10 2022-12-20 江苏省中国科学院植物研究所 Carbon-based microbial soil conditioner and preparation method and application thereof
CN116947562A (en) * 2023-06-20 2023-10-27 江西沃邦农业科技股份有限公司 Novel crop fertilizer and preparation method thereof

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1699298A (en) * 2004-05-21 2005-11-23 汉城精细技术有限公司 PGPR growth accelerant and method for culturing agronomic crop using it
CN105950509A (en) * 2016-05-27 2016-09-21 河北科技师范学院 Biological fungicide and preparation method as well as application thereof
CN108503487A (en) * 2018-04-26 2018-09-07 史丹利农业集团股份有限公司 A kind of stable fertilizer and preparation method thereof of addition water-soluble poly ammonium phosphate
CN110183272A (en) * 2019-06-10 2019-08-30 中科绿洲(北京)生态工程技术有限公司 A kind of high and cold arid desert land ecology reparation soil conditioner and ecological restoring method
CN110373207A (en) * 2019-07-29 2019-10-25 苏州沣懿生物科技有限公司 A kind of microbial bacterial agent and its preparation process of alkaline land improving
CN112794764A (en) * 2021-02-03 2021-05-14 四川农业大学 Complete-element biological organic fertilizer and preparation method thereof
CN113582762A (en) * 2021-08-19 2021-11-02 宁夏农林科学院农业资源与环境研究所(宁夏土壤与植物营养重点实验室) Compound microbial fertilizer special for saline-alkali soil alfalfa and preparation method thereof
CN113698245A (en) * 2021-09-09 2021-11-26 河南农业大学 Biological organic fertilizer for improving sand ginger soil and preparation method and application thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1699298A (en) * 2004-05-21 2005-11-23 汉城精细技术有限公司 PGPR growth accelerant and method for culturing agronomic crop using it
CN105950509A (en) * 2016-05-27 2016-09-21 河北科技师范学院 Biological fungicide and preparation method as well as application thereof
CN108503487A (en) * 2018-04-26 2018-09-07 史丹利农业集团股份有限公司 A kind of stable fertilizer and preparation method thereof of addition water-soluble poly ammonium phosphate
CN110183272A (en) * 2019-06-10 2019-08-30 中科绿洲(北京)生态工程技术有限公司 A kind of high and cold arid desert land ecology reparation soil conditioner and ecological restoring method
CN110373207A (en) * 2019-07-29 2019-10-25 苏州沣懿生物科技有限公司 A kind of microbial bacterial agent and its preparation process of alkaline land improving
CN112794764A (en) * 2021-02-03 2021-05-14 四川农业大学 Complete-element biological organic fertilizer and preparation method thereof
CN113582762A (en) * 2021-08-19 2021-11-02 宁夏农林科学院农业资源与环境研究所(宁夏土壤与植物营养重点实验室) Compound microbial fertilizer special for saline-alkali soil alfalfa and preparation method thereof
CN113698245A (en) * 2021-09-09 2021-11-26 河南农业大学 Biological organic fertilizer for improving sand ginger soil and preparation method and application thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115491205A (en) * 2022-08-10 2022-12-20 江苏省中国科学院植物研究所 Carbon-based microbial soil conditioner and preparation method and application thereof
CN116947562A (en) * 2023-06-20 2023-10-27 江西沃邦农业科技股份有限公司 Novel crop fertilizer and preparation method thereof

Similar Documents

Publication Publication Date Title
CN109400372B (en) Biochar soil improvement organic fertilizer and preparation method thereof
CN107365588B (en) Special drought-resistant water-retaining self-loosening water-retaining agent and matrix for scindapsus aureus and preparation method of special drought-resistant water-retaining self-loosening water-retaining agent and matrix
CN102329754A (en) Novel strain of streptomyces fradiae and application thereof
CN114455999A (en) Special microbial agent for newly-repaired terrace soil in loess hilly area and preparation method thereof
CN112442462B (en) Composite microbial inoculum for straw returning and decomposing, preparation method and application thereof
CN110791441A (en) Bacillus amyloliquefaciens and method for preparing gamma-polyglutamic acid fertilizer synergist by using same
CN110156541A (en) A kind of restorative procedure of secondary salinization soil
CN111919709A (en) Cow dung basic carrier culture medium containing plant growth-promoting bacteria and preparation method thereof
CN106068746A (en) A kind of salt-soda soil biological modification method
CN112624866A (en) Organic slow release fertilizer using livestock and poultry manure and straw as raw materials
CN108409486A (en) A kind of composite biological fertilizer suitable for slope collapse erosion land soil
CN111499430A (en) Humic acid compound fertilizer for improving tobacco-planting soil and preparation method thereof
CN108476880B (en) Method for rapidly planting and strengthening roots of pepper seedlings
CN112266279A (en) Preparation method and application of organic fertilizer for improving richness and diversity of soil microbial flora
CN114342729B (en) Organic planting method for pecans
CN110981636A (en) Sand land water-saving fertilizer-maintaining material based on homologous plants and application thereof
CN110447493A (en) A kind of method improving peanut yield and the peanut cultivation method using this method
CN114342629A (en) Rapid cultivation method for new terrace soil in loess hilly area
CN110506597B (en) Method for promoting well-cellar type transplanting of rice field tobacco plants to early and quickly grow
CN112430163A (en) Biological fertilizer for relieving continuous cropping obstacle of watermelon with pressed sand
CN111646855A (en) Special charcoal-based compound fertilizer for honey peaches and fertilizing method thereof
CN110999743A (en) Planting method for micro-fertilizer high-yield peanuts
CN118303287B (en) Method for planting third-generation lily bulbs by soil improvement and biological fertilizer used by same
Ezung et al. Performance of greengram as influenced by different levels of vermicompost+ rhizobium and spacing on growth, yield and economics under upland terrace condition of north east India
CN109796267A (en) A kind of dedicated full nutrition slow-release cultivation matrix of muskmelon and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20220510

RJ01 Rejection of invention patent application after publication