CN102272333A - 利用等位基因特异性抑制序列变体的核酸扩增 - Google Patents

利用等位基因特异性抑制序列变体的核酸扩增 Download PDF

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Publication number
CN102272333A
CN102272333A CN2010800043562A CN201080004356A CN102272333A CN 102272333 A CN102272333 A CN 102272333A CN 2010800043562 A CN2010800043562 A CN 2010800043562A CN 201080004356 A CN201080004356 A CN 201080004356A CN 102272333 A CN102272333 A CN 102272333A
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Prior art keywords
oligonucleotide
target sequence
nucleic acid
amplification
variants
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CN2010800043562A
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Chinese (zh)
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M·格罗
V·布罗菲
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Priority to CN201710109955.4A priority Critical patent/CN106702000A/zh
Publication of CN102272333A publication Critical patent/CN102272333A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN2010800043562A 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增 Pending CN102272333A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710109955.4A CN106702000A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14311309P 2009-01-07 2009-01-07
US61/143113 2009-01-07
PCT/EP2010/000030 WO2010079127A1 (en) 2009-01-07 2010-01-07 Nucleic acid amplification with allele-specific suppression of sequence variants

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CN201710109955.4A Division CN106702000A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

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CN102272333A true CN102272333A (zh) 2011-12-07

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CN2010800043562A Pending CN102272333A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增
CN201710109955.4A Pending CN106702000A (zh) 2009-01-07 2010-01-07 利用等位基因特异性抑制序列变体的核酸扩增

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Country Link
US (1) US8206929B2 (enExample)
EP (1) EP2385991B1 (enExample)
JP (1) JP5785095B2 (enExample)
CN (2) CN102272333A (enExample)
CA (1) CA2747068C (enExample)
ES (1) ES2634428T3 (enExample)
WO (1) WO2010079127A1 (enExample)

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WO2023025218A1 (zh) * 2021-08-25 2023-03-02 南京金斯瑞生物科技有限公司 一种用于检测单碱基突变的引物组和基因芯片方法

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ES2662825T3 (es) 2013-02-25 2018-04-09 Seegene, Inc. Detección de variación de nucleótido en una secuencia de ácidos nucleicos diana
US20160244816A1 (en) 2013-07-15 2016-08-25 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
US11447814B2 (en) 2013-10-18 2022-09-20 Seegene, Inc. Detection of target nucleic acid sequence on solid phase by PTO cleavage and extension using HCTO assay
JP2016535987A (ja) 2013-10-20 2016-11-24 トローバジーン インコーポレイテッド 核酸配列の合成及び濃縮
RU2708992C2 (ru) * 2014-05-19 2019-12-12 Уильям Марш Райс Юниверсити Аллель-специфическая амплификация с использованием композиции перекрывающихся олигонуклеотидов в качестве не являющегося аллель-специфическим праймера и аллель-специфического блокатора
JP5813263B1 (ja) * 2015-03-31 2015-11-17 日鉄住金環境株式会社 遺伝子変異の検出方法及びそれに用いる蛍光標識オリゴヌクレオチド
EP3551756A4 (en) 2016-12-12 2020-07-15 Dana Farber Cancer Institute, Inc. COMPOSITIONS AND METHODS FOR MOLECULAR BARCODE CODING OF DNA MOLECULES BEFORE ENRICHMENT OF MUTATIONS AND / OR DETECTION OF MUTATIONS
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
EP4402283A4 (en) * 2021-09-13 2025-11-12 Nexgen Cancer Detection Llc METHODS FOR DETECTING A TARGET POLYNUCLEOTIDE IN A SAMPLE

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023025218A1 (zh) * 2021-08-25 2023-03-02 南京金斯瑞生物科技有限公司 一种用于检测单碱基突变的引物组和基因芯片方法

Also Published As

Publication number Publication date
WO2010079127A1 (en) 2010-07-15
US20100173311A1 (en) 2010-07-08
CN106702000A (zh) 2017-05-24
JP2012514451A (ja) 2012-06-28
US8206929B2 (en) 2012-06-26
CA2747068A1 (en) 2010-07-15
EP2385991B1 (en) 2017-05-17
EP2385991A1 (en) 2011-11-16
JP5785095B2 (ja) 2015-09-24
CA2747068C (en) 2014-04-08
ES2634428T3 (es) 2017-09-27

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