CN102217567A - In-vitro culture technology for fertilized ova of beautiful white shrimps - Google Patents
In-vitro culture technology for fertilized ova of beautiful white shrimps Download PDFInfo
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- CN102217567A CN102217567A CN2011101454741A CN201110145474A CN102217567A CN 102217567 A CN102217567 A CN 102217567A CN 2011101454741 A CN2011101454741 A CN 2011101454741A CN 201110145474 A CN201110145474 A CN 201110145474A CN 102217567 A CN102217567 A CN 102217567A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses the in-vitro culture technology for fertilized ova of beautiful white shrimps, which is characterized by comprising the following steps of: preparing incubating water, selecting brooding shrimps, separating parental shrimps from the ova, disinfecting, incubating, managing daily and the like. Parents of the beautiful white shrimps are easy to die in the processes of catching and transportation, so the ova of the brooding shrimps which just died can be subjected to in-vitro incubation to save the cost of purchase of the parents, and the shrimp ova are more convenient to transport; part of parent shrimps also can be prevented from nibbling shrimp seedlings after being bred, so that the survival rate of the shrimp seedlings is improved; and the beautiful white shrimps of which the ova are cut also can be used for preparing dried shrimps to avoid the waste of resources.
Description
Technical field
The present invention relates to a kind of a kind of aquaculture technology that the present invention relates to, particularly relate to the stripped cultivation technology in batches of beautiful white shrimp fertilized egg.
Background technology
Beautiful white shrimp (
Exopalaemon modestus) be under the jurisdiction of Crustachia, Decapoda, Palaemonidae, Palaemon, be distributed widely in China's freshwater lake and the river, all produce in north and south.In the shrimps in Taihu Lake were formed, beautiful white shrimp accounted for more than 50%.As one of main economic fishery harvesting object in Taihu Lake " Taihu Lake three is white ", no matter still be the output value from output on, beautiful white shrimp occupies important status in the fishery of Taihu Lake.In recent years, on the one hand along with ring continuous development of Taihu Lake industry and being on the increase of population, Taihu Lake water quality pollution becoming serious, the procreation of the beautiful white shrimp in Taihu Lake, existence have been subjected to very big threat; On the other hand, the demand of market dialogue shrimp increases day by day, and extravagent price lures that fisherman along the lake ignores white shrimp current resources situation and fishes for desperately into, and therefore white shrimp species matter protection of resources and aquaculture are extremely urgent.
Since beautiful white shrimp parent fish for, very easily dead in the transportation, this has strengthened virtually grows seedlings and aquaculture cost; The development degree of the beautiful white shrimp shrimp with ovums fertilized egg of fishing for is also inequality, and the early growth of shrimp seedling is very fast, and therefore the shrimp seedling that hatches after the shrimp seedling that hatches earlier very easily kills and devour is necessary to carry out the stripped batch of beautiful white shrimp fertilized egg and cultivates technical research.
The research of at present relevant beautiful white shrimp mainly concentrates on aspects such as growth, feeding habits, cell biology, oxygen consumption, and seedling growing process rarely has report, and the fertilized egg in-vitro breeding technique is blank especially.
Summary of the invention
The present invention is for a kind of beautiful white shrimp fertilized egg in-vitro hatching technology of saving seedling cost and reaching the purpose of growing seedlings in batches is provided.
The present invention adopts following technical scheme for achieving the above object:
A kind of beautiful white shrimp fertilized egg in-vitro breeding technique may further comprise the steps:
(1) hatching water preparation: fully the running water behind the aeration is standby behind 70-80 purpose nylon silk cover filtering, and temperature is that 20-30 ℃, pH are between 7.0-8.3;
(2) preparation of incubation vessel: select smooth glass or the plastics bulge of inner face, the micropore oxygen increasing tube is settled in the bottom, and micropore oxygen increasing tube layout in the form of annular discs taps into tracheae, and air inlet pipe is connected with the electromagnetism oxygen increasing pump after connecing air valve;
(3) choosing of shrimp with ovums: in late May-July, select more than the long 30mm of body, physically well develop, body surface does not have parasite, body colour is transparent fresh and alive or death but shrimp with ovums that body colour does not bleach;
(4) separating of close shrimp and ovum: be close to belly with sterile scissors and carefully cut off appendage and two avris first, close scissors, in the middle of belly, provoke with the direction of scissors front end edge tail-head;
(5) sterilization of shrimp ovum: the shrimp ovum is put into aseptic circular dishware, add 7 ‰-10 ‰ sodium chloride solution, soaked 5-10 minute;
(6) hatching: by 500 string shrimp ovum in every cubic metre of water is the density of ten thousand ovum of about 15-25 in every cubic metre of water, to put into the incubation vessel that fills hatching water through the shrimp ovum that step (4) is handled, regulate air inflow, make the shrimp ovum in water, slightly roll, illumination every day 5-12 hours, intensity of illumination is 100-1000Lux, and 10-25 is after day, shrimp ovum incubation of membrane;
(7) management: be used alternatingly every 4 hours with two electromagnetism oxygen increasing pumps, thoroughly change water once every 24 hours, turn off oxygen increasing pump before changing water, when treating that the shrimp ovum is deposited to container bottom, the water body on top is fallen in siphon, then the fresh hatching water of replenish step (1) preparation.
Described hatching water temperature in the preferred step (1) is controlled at 25-28 ℃, and pH is between the 7.2-7.6.
The body of shrimp with ovums described in the preferred step (3) is long to be 35-45mm, and absolute oogenesis amount is more than 350.
Step is classified shrimp with ovums according to the color of shrimp ovum in (3), and the shrimp ovum that guarantees same color is in same incubator, thus the synchronism that the assurance hatching is emerged.
Intensity of illumination is preferably 300-600lux described in the preferred step (6).
The present invention has following beneficial effect:
(1) the present invention can save parent's acquisition cost.
(2) the present invention can reduce the transportation loss.
(3) the incubation rate height of shrimp ovum of the present invention.
(4) neat specification of shrimp seedling of the present invention effectively avoids gobbling little phenomenon.
Embodiment
A kind of beautiful white shrimp fertilized egg in-vitro breeding technique may further comprise the steps:
(1) hatching water preparation: fully the running water behind the aeration is standby behind 70-80 purpose nylon silk cover filtering, and temperature is that 20-30 ℃, pH are between 7.0-8.3;
(2) preparation of incubation vessel: select smooth glass or the plastics bulge of inner face, the micropore oxygen increasing tube is settled in the bottom, and micropore oxygen increasing tube layout in the form of annular discs taps into tracheae, and air inlet pipe is connected with the electromagnetism oxygen increasing pump after connecing air valve;
(3) choosing of shrimp with ovums: in late May-July, select more than the long 30mm of body, physically well develop, body surface does not have parasite, body colour is transparent fresh and alive or death but shrimp with ovums that body colour does not bleach;
(4) separating of close shrimp and ovum: be close to belly with sterile scissors and carefully cut off appendage and two avris first, close scissors, in the middle of belly, provoke with the direction of scissors front end edge tail-head;
(5) sterilization of shrimp ovum: the shrimp ovum is put into aseptic circular dishware, add 7 ‰-10 ‰ sodium chloride solution, soaked 5-10 minute;
(6) hatching: by 500 string shrimp ovum in every cubic metre of water is the density of ten thousand ovum of about 15-25 in every cubic metre of water, to put into the incubation vessel that fills hatching water through the shrimp ovum that step (4) is handled, regulate air inflow, make the shrimp ovum in water, slightly roll, illumination every day 5-12 hours, intensity of illumination is 100-1000Lux, and 10-25 is after day, shrimp ovum incubation of membrane;
(7) management: be used alternatingly every 4 hours with two electromagnetism oxygen increasing pumps, thoroughly change water once every 24 hours, turn off oxygen increasing pump before changing water, when treating that the shrimp ovum is deposited to container bottom, the water body on top is fallen in siphon, then the fresh hatching water of replenish step (1) preparation.
Described hatching water temperature in the preferred step (1) is controlled at 25-28 ℃, and pH is between the 7.2-7.6.
The body of shrimp with ovums described in the preferred step (3) is long to be 35-45mm, and absolute oogenesis amount is more than 350.
Step is classified shrimp with ovums according to the color of shrimp ovum in (3), and the shrimp ovum that guarantees same color is in same incubator, thus the synchronism that the assurance hatching is emerged.
Intensity of illumination is preferably 300-600lux described in the preferred step (6).
Claims (5)
1. beautiful white shrimp fertilized egg in-vitro breeding technique may further comprise the steps:
(1) hatching water preparation: fully the running water behind the aeration is standby behind 70-80 purpose nylon silk cover filtering, and temperature is that 20-30 ℃, pH are between 7.0-8.3;
(2) preparation of incubation vessel: select smooth glass or the plastics bulge of inner face, the micropore oxygen increasing tube is settled in the bottom, and micropore oxygen increasing tube layout in the form of annular discs taps into tracheae, and air inlet pipe is connected with the electromagnetism oxygen increasing pump after connecing air valve;
(3) choosing of shrimp with ovums: in late May-July, select more than the long 30mm of body, physically well develop, body surface does not have parasite, body colour is transparent fresh and alive or death but shrimp with ovums that body colour does not bleach;
(4) separating of close shrimp and ovum: be close to belly with sterile scissors and carefully cut off appendage and two avris first, close scissors, in the middle of belly, provoke with the direction of scissors front end edge tail-head;
(5) sterilization of shrimp ovum: the shrimp ovum is put into aseptic circular dishware, add 7 ‰-10 ‰ sodium chloride solution, soaked 5-10 minute;
(6) hatching: by 500 string shrimp ovum in every cubic metre of water is the density of ten thousand ovum of about 15-25 in every cubic metre of water, to put into the incubation vessel that fills hatching water through the shrimp ovum that step (4) is handled, regulate air inflow, make the shrimp ovum in water, slightly roll, illumination every day 5-12 hours, intensity of illumination is 100-1000Lux, and 10-25 is after day, shrimp ovum incubation of membrane;
(7) management: be used alternatingly every 4 hours with two electromagnetism oxygen increasing pumps, thoroughly change water once every 24 hours, turn off oxygen increasing pump before changing water, when treating that the shrimp ovum is deposited to container bottom, the water body on top is fallen in siphon, then the fresh hatching water of replenish step (1) preparation.
2. a kind of beautiful white shrimp fertilized egg in-vitro breeding technique according to claim 1, it is characterized in that: the described hatching water temperature in the step (1) is controlled at 25-28 ℃, and pH is preferably between the 7.2-7.6.
3. a kind of beautiful white shrimp fertilized egg in-vitro breeding technique according to claim 1, it is characterized in that: the body length of shrimp with ovums is preferably 35-45mm described in the step (3), and absolute oogenesis amount is more than 350.
4. a kind of beautiful white shrimp fertilized egg in-vitro breeding technique according to claim 1, it is characterized in that: step is classified shrimp with ovums according to the color of shrimp ovum in (3), the shrimp ovum that guarantees same color is in same incubator, thus the synchronism that the assurance hatching is emerged.
5. a kind of beautiful white shrimp fertilized egg in-vitro breeding technique according to claim 1, it is characterized in that: intensity of illumination is preferably 300-600lux described in the step (6).
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715116A (en) * | 2012-07-10 | 2012-10-10 | 中国科学院广州生物医药与健康研究院 | Zebra fish breeding method |
| CN103004671A (en) * | 2013-01-11 | 2013-04-03 | 中国水产科学研究院黄海水产研究所 | Method for manually hatching palaemon carinicauda indoors and preferentially selecting larvas |
| CN103270918A (en) * | 2013-05-10 | 2013-09-04 | 常熟市银湖生态农业有限公司 | Rice and shrimp joint culturing ecological breeding system and method |
| CN103314908A (en) * | 2013-06-21 | 2013-09-25 | 太仓年年有渔农业科技有限公司 | Isolated breeding technique for cherax quadricarinatus eggs |
| CN103636541A (en) * | 2013-12-18 | 2014-03-19 | 上海市水产研究所 | In-vitro incubation method for fertilized eggs of macrobrachium rosenbergii |
| CN103960182A (en) * | 2014-04-28 | 2014-08-06 | 江苏省海洋水产研究所 | Method for establishing exopalaemon carinicauda family by applying in-vitro egg culture |
| CN105557590A (en) * | 2016-01-18 | 2016-05-11 | 琼海胜达种植养殖场 | Australian cherax quadricarinatus in-vitro hatching method |
| CN107361002A (en) * | 2017-09-21 | 2017-11-21 | 江苏盱眙满江红龙虾产业园有限公司 | A kind of Procambrus clarkii ovum in-vitro culture method |
| CN107412271A (en) * | 2017-06-16 | 2017-12-01 | 浙江省淡水水产研究所 | Disinfectant for shrimp ovum |
| CN108605875A (en) * | 2018-05-04 | 2018-10-02 | 江苏螯龙水产养殖科技有限公司 | A kind of red huge legendary turtle huge legendary turtle shrimp oogenesis ex vivo approach |
| CN110250056A (en) * | 2019-07-10 | 2019-09-20 | 淮阴师范学院 | A method of improving Macrobrachium nipponensis fertilized eggs in-vitro hatching rate |
| CN111328742A (en) * | 2020-03-03 | 2020-06-26 | 天津农学院 | Method for improving hatching rate of in-vitro culture of embryos of young shrimps with saw teeth |
| CN111758625A (en) * | 2020-06-29 | 2020-10-13 | 中国水产科学研究院淡水渔业研究中心 | Method for industrial in-vitro breeding of procambarus clarkii |
| CN111758626A (en) * | 2020-06-29 | 2020-10-13 | 中国水产科学研究院淡水渔业研究中心 | Method for controlling development of in-vitro fertilized egg embryo of procambarus clarkii |
| CN115005134A (en) * | 2022-06-14 | 2022-09-06 | 海南省菜篮农业与渔业发展有限公司 | In-vitro hatching method for freshwater shrimps |
| CN115088656A (en) * | 2022-07-13 | 2022-09-23 | 珠海市海之亿水产科技有限责任公司 | Shrimp fry cultivation method |
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Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715116A (en) * | 2012-07-10 | 2012-10-10 | 中国科学院广州生物医药与健康研究院 | Zebra fish breeding method |
| CN103004671A (en) * | 2013-01-11 | 2013-04-03 | 中国水产科学研究院黄海水产研究所 | Method for manually hatching palaemon carinicauda indoors and preferentially selecting larvas |
| CN103270918A (en) * | 2013-05-10 | 2013-09-04 | 常熟市银湖生态农业有限公司 | Rice and shrimp joint culturing ecological breeding system and method |
| CN103270918B (en) * | 2013-05-10 | 2014-11-05 | 常熟市银湖生态农业有限公司 | Rice and shrimp joint culturing ecological breeding system and method |
| CN103314908A (en) * | 2013-06-21 | 2013-09-25 | 太仓年年有渔农业科技有限公司 | Isolated breeding technique for cherax quadricarinatus eggs |
| CN103636541A (en) * | 2013-12-18 | 2014-03-19 | 上海市水产研究所 | In-vitro incubation method for fertilized eggs of macrobrachium rosenbergii |
| CN103960182A (en) * | 2014-04-28 | 2014-08-06 | 江苏省海洋水产研究所 | Method for establishing exopalaemon carinicauda family by applying in-vitro egg culture |
| CN105557590A (en) * | 2016-01-18 | 2016-05-11 | 琼海胜达种植养殖场 | Australian cherax quadricarinatus in-vitro hatching method |
| CN107412271B (en) * | 2017-06-16 | 2020-09-22 | 浙江省淡水水产研究所 | Disinfectant for shrimp eggs |
| CN107412271A (en) * | 2017-06-16 | 2017-12-01 | 浙江省淡水水产研究所 | Disinfectant for shrimp ovum |
| CN107361002A (en) * | 2017-09-21 | 2017-11-21 | 江苏盱眙满江红龙虾产业园有限公司 | A kind of Procambrus clarkii ovum in-vitro culture method |
| CN108605875A (en) * | 2018-05-04 | 2018-10-02 | 江苏螯龙水产养殖科技有限公司 | A kind of red huge legendary turtle huge legendary turtle shrimp oogenesis ex vivo approach |
| CN110250056A (en) * | 2019-07-10 | 2019-09-20 | 淮阴师范学院 | A method of improving Macrobrachium nipponensis fertilized eggs in-vitro hatching rate |
| CN110250056B (en) * | 2019-07-10 | 2021-09-24 | 淮阴师范学院 | Method for improving in vitro hatchability of fertilized eggs of macrobrachium nipponense |
| CN111328742A (en) * | 2020-03-03 | 2020-06-26 | 天津农学院 | Method for improving hatching rate of in-vitro culture of embryos of young shrimps with saw teeth |
| CN111328742B (en) * | 2020-03-03 | 2021-12-14 | 天津农学院 | Method for improving hatching rate of in-vitro culture of embryos of young shrimps with saw teeth |
| CN111758625A (en) * | 2020-06-29 | 2020-10-13 | 中国水产科学研究院淡水渔业研究中心 | Method for industrial in-vitro breeding of procambarus clarkii |
| CN111758626A (en) * | 2020-06-29 | 2020-10-13 | 中国水产科学研究院淡水渔业研究中心 | Method for controlling development of in-vitro fertilized egg embryo of procambarus clarkii |
| CN115005134A (en) * | 2022-06-14 | 2022-09-06 | 海南省菜篮农业与渔业发展有限公司 | In-vitro hatching method for freshwater shrimps |
| CN115088656A (en) * | 2022-07-13 | 2022-09-23 | 珠海市海之亿水产科技有限责任公司 | Shrimp fry cultivation method |
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Application publication date: 20111019 |