CN102715116B - Zebra fish breeding method - Google Patents
Zebra fish breeding method Download PDFInfo
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- CN102715116B CN102715116B CN2012102370273A CN201210237027A CN102715116B CN 102715116 B CN102715116 B CN 102715116B CN 2012102370273 A CN2012102370273 A CN 2012102370273A CN 201210237027 A CN201210237027 A CN 201210237027A CN 102715116 B CN102715116 B CN 102715116B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to a zebra fish breeding method. The method comprises the steps of closing a circulating water switch of a fish tank needing for feeding before feeding for 20-30 minutes; feeding incubated parva or proper solid food into the fish tank; and after feeding for 20 minutes, turning on the closed circulating water switch. The method can ensure the daily growth health of zebra fish, meet the growth rules of the zebra fish, and ensure the high-quality survival and growth of the zebra fish.
Description
Technical field
The invention belongs to the animal feeding field, particularly relate to the raising method of a kind of zebra fish.
Background technology
Zebra fish (Danio rerio claims zebra fish again) is subordinate to Cyprinidae, short hundredweight Buddhist nun fish belongs to, and is commonly called as Blue Streak fish, flower bar fish, zebra load Buddhist nun fish, for originating in a kind of low profile thermal hairtail in waters, ground such as India, Bangladesh.Zebra fish and mouse, rat isotype biota ratio, its have individual little, be easy to raise, therefore characteristics such as observation are utilized zebra fish to do experimental study to have that experimental cost is low, experimental period short, are easy to characteristics such as the breeding cycle is short, egg laying amount is high, in vitro fertilization.
The optimum growth of zebra fish and breeding temperature are below or above this temperature between 24-30 ℃, the breeding of zebra fish and embryonic development all can't normally be carried out.Because zebra fish can be dead when 11 ℃ of left and right sides, so its more sad winter under natural conditions in most of area at home.Zebra fish belongs to ominivore-fish.Under the condition of artificial feeding, the young is food with the nauplius of rubbing broken yolk, wheel animalcule, halogen worm with the hands, adult then can throw something and feed ordinary sheet feed stuff for pet fish, nematode, red worm, fairy shrimp etc.In the daily management of zebra fish, zebra fish is not very high to requirements in water quality, is advisable with alkalescent, pH value 7-8.Can culture with running water in the laboratory, but used running water is wanted aeration 1d at least.Answer residual bait and ight soil in the sucking-off fish jar every day, and change water about 1/3 at least, or use equipment for filtering water.
Zebra fish needs oxygen hardly in its incubation period, but along with it is constantly grown up, and the demand of oxygen is increased gradually.Zebra fish is owing to have higher accretion rate, so the amount of oxygen that its unit volume consumes will be higher than other large-scale fish.In the process that culture in the laboratory, oxygenation is conducive to the growth of zebra fish in the water body.Aspect feeding, throw something and feed 2-3 every day, and the parent population that is breeding can add on this basis and feeds once, and each feeding volume is eaten up in 5m in zebra fish and is advisable.Zebra fish is less demanding to bait, no matter is that artificial synthetic diet or various fresh food are all passable.But in order to guarantee that zebra fish can grow and breed preferably, best multiple bait is in conjunction with throwing something and feeding.For the zebra fish that is breeding, need every day especially and throw something and feed at least the once fresh food such as fairy shrimp.As long as the raising condition is suitable, zebra fish is generally seldom fallen ill.
If but condition of water quality is bad or to change water not diligent, zebra fish also bacterial infection can occur, is embodied in symptoms such as mashed tail, gill rot, mashed fin, desquamation, perpendicular squama and dermohemia inflammation.The zebra fish of falling ill in time be chosen, and carries out dipping with the potassium permanganate dilution.If carry out toxicology or pharmacological experiment with zebra fish, then in time eliminate sick fish, can impact experimental result because heal with medicine.In order to deal with emergency case, to notice that before the experiment beginning many some zebra fishs of breed are standby, to prevent influencing the experiment progress because eliminating sick fish.In daily breeding process, used daily apparatus such as fishnet etc. will wash earlier after using up, then with liquor potassic permanganate or the sterilization of 84 medicining liquid dippings, in order to using next time.For fish jar, preferably can clean once by every two weeks.The characteristic of zebra fish, the build of morphological feature zebra fish is spindle, and the adult fish body is about 4-6cm, and the side has and the similar vertically Beijing enamel ware vitta line of zebra, and the back is olive colour, on anal fin and tail fin the striped similar to the side is arranged also.
Above-mentioned method of the prior art all exists the survival rate of raising zebra fish low, have in addition can only survive 50%, for the experiment of using zebra fish, cost increases greatly, even the influence experiment progress that needs zebra fish to carry out.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of zebra fish raising method on duty for the day, this method comprises the steps:
In morning, evening:
1. before feeding fish 20-30 minute, close the cycle water switch of the fish jar that needs feeding;
2. select corresponding shrimp hatching cylinder, gas stone is taken out from the hatching cylinder, died 5-10 minute.
The time of dying is unsuitable long, and long meeting makes shrimp dead because of anoxic, and can make the discharge of shrimp not smooth because a large amount of shrimps accumulates in the discharge outlet place, dies and is advisable in time 5-10 minute;
3. receive shrimp: after waiting to hatch shrimp in the cylinder and sinking to the bottom, disconnect the heating rod power supply, collect shrimp (current are little, and the shrimp of Liu Chuing abandons not at first, remove the shrimp water in the dry screen net as far as possible) with fine screen mesh, with system water shrimp is dissolved in 1 liter of beaker, stir evenly; Wait that the shrimp ovum (black particle) of not hatching all sinks to the bottom, and behind the shrimp shell with fine screen mesh removing water surface (careful operation, dynamics are crossed the shrimp ovum that the bottom is rolled in conference);
4. feed fish: the shrimp that will remove behind the shrimp shell is poured in the wash bottle, is diluted to suitable concentration and feeds fish.
Note the shrimp ovum of beaker bottom not being poured in the wash bottle, may stop up dead because of digestive system behind the edible shrimp ovum of fish;
5. boiling water: after feeding 20-30 minute, with the cycle water switch opens of closing before;
6. wash cylinder; Clean hatching cylinder and heating rod with pure water;
7. claim shrimp ovum and salt: the shrimp ovum 3-5 grams per liter that scale is good, sea salt 13-16 grams per liter, shrimp ovum add pure water and soak, ventilation dissolving behind 1 liter of pure water of sea salt adding;
8. incubate shrimp: after cleaning, with oneself sea salt through dissolving, (residue of beaker bottom discard need not), the shrimp ovum that soaked is poured in the hatching cylinder, adds suitable pure water, and heating rod, gas stone, temperature probe are installed, connect the heating rod power supply, begin to incubate shrimp, treat to feed next time fish and use;
9. cleaning; Used utensil cleaning is finished cleaning desktop, pond, ground;
At noon:
1. before feeding fish 20-30 minute, close the cycle water switch of the fish jar that needs feeding;
2. an amount of food is dropped in the fish jar;
3. after feeding 20-30 minute, with the cycle water switch opens of closing before;
In the said method,
Preferably, zebra fish need feed the morning, noon and afternoon every day and be respectively for three times: 8:30, and 12:30,16:30,
Preferably, the incubation condition of shrimp is 29 ℃, continuous culture 24 hours;
Preferably, before the shrimp ovum will be used up, in advance the canned shrimp ovum in the refrigerator is taken out, after room temperature is placed 12 hours, use;
Preferably, will be with rubber gloves when cleaning incubator, careful hand is scratched;
Preferably, clean and will note heating rod not being smashed when laying heating rod;
Preferably, after feeding fish and finishing 20-30 minute, open the cycle water switch after, check the water inlet pipe in the fish jar, water pipe should be more than liquid level at least 1 centimeters, current are advisable with the maximum stream flow that does not produce bubble;
Preferably, in the said method, scale shrimp and salt: shrimp ovum 3 or 5 grams per liters, sea salt 13 or 16 grams per liters.
The food that feed fish noon is commercially available zebra fish feed, perhaps Chang Gui zebra fish food, as comprise the protein greater than 40wt%, greater than 3.5wt% crude fat, moisture is lower than 4%, calcium 0.9%～1.6%, phosphorus 0.8%～1.4%, and add in right amount to the effective material of gonad development, as VE, VA, VD, trace element zinc etc.
Method of the present invention can guarantee the health of the daily growth of zebra fish, meets the growth rhythm of zebra fish, can guarantee the high-quality survival of zebra fish and growth.
Embodiment
In order to understand the present invention, further specify the present invention with embodiment below, but do not limit the present invention.
The raising on duty for the day of embodiment 1 zebra fish
The morning:
1. at 8:10, close the cycle water switch of the fish jar that needs feeding;
2. the hatching cylinder of select time correspondence takes out gas stone from the hatching cylinder, dies 5 minutes time;
3. receive shrimp: after waiting to hatch shrimp in the cylinder and sinking to the bottom, disconnect the heating rod power supply, collect shrimp (current are little, and the shrimp of Liu Chuing abandons not at first) with fine screen mesh, remove the shrimp water in the dry screen net as far as possible, with system water shrimp is dissolved in 1 liter of beaker, stir evenly; Wait that the shrimp ovum (black particle) of not hatching all sinks to the bottom, and clean except (careful operation, dynamics are crossed the shrimp ovum that the bottom is rolled in conference) behind the shrimp shell of water surface with fine screen mesh;
4.8:30 feed fish: the shrimp that will remove behind the shrimp shell is poured in the wash bottle, is diluted to suitable concentration and feeds fish, notes the shrimp ovum of beaker bottom not being poured in the wash bottle, may be because of digestive system obstruction death behind the edible shrimp ovum of fish;
5. boiling water: at feeding after 20 minutes, with the cycle water switch opens of closing before;
6. wash cylinder; Clean hatching cylinder and heating rod with pure water;
7. shrimp ovum 3 grams per liters that scale is good, sea salt 13 grams per liters, shrimp ovum add pure water and soak, and ventilation was dissolved after sea salt added 1 liter of pure water;
8. incubate shrimp: after cleaning, with oneself sea salt through dissolving, (residue of beaker bottom discard need not), the shrimp ovum that soaked is poured in the hatching cylinder, add suitable pure water, heating rod, gas stone, temperature probe are installed, and connect the heating rod power supply: the incubation condition of shrimp is 29 ℃, continuous culture 24 hours.
9. cleaning; Used utensil cleaning is finished cleaning desktop, pond, ground;
Noon:
1. at 12:10, close the cycle water switch of the fish jar that needs feeding;
2.12:30, an amount of food is dropped in the fish jar, food is tropical fish feeding conventional food;
3. at feeding after 20 minutes, with the cycle water switch opens of closing before;
Afternoon:
1. at 16:10, close the cycle water switch of the fish jar that needs feeding;
2. ensuing operating procedure is with the operating procedure in the morning, and just asynchronism(-nization) is fed fish at 16:30; Feed behind the fish and to open cycle water in 20 minutes.
The raising on duty for the day of embodiment 2 zebra fishs
The morning:
1. at 8:00, close the cycle water switch of the fish jar that needs feeding;
2. the hatching cylinder of select time correspondence takes out gas stone from the hatching cylinder, dies 10 minutes time;
3. receive shrimp: after waiting to hatch shrimp in the cylinder and sinking to the bottom, disconnect the heating rod power supply, collect shrimp (current are little, and the shrimp of Liu Chuing abandons not at first) with fine screen mesh, remove the shrimp water in the dry screen net as far as possible, with system water shrimp is dissolved in 1 liter of beaker, stir evenly; Wait that the shrimp ovum (black particle) of not hatching all sinks to the bottom, and clean except (careful operation, dynamics are crossed the shrimp ovum that the bottom is rolled in conference) behind the shrimp shell of water surface with fine screen mesh;
4.8:30 feed fish: the shrimp that will remove behind the shrimp shell is poured in the wash bottle, is diluted to suitable concentration and feeds fish, notes the shrimp ovum of beaker bottom not being poured in the wash bottle, may be because of digestive system obstruction death behind the edible shrimp ovum of fish;
5. boiling water: at feeding after 30 minutes, with the cycle water switch opens of closing before;
6. wash cylinder; Clean hatching cylinder and heating rod with pure water;
7. shrimp ovum 4 grams per liters that scale is good, sea salt 15 grams per liters, shrimp ovum add pure water and soak, and ventilation was dissolved after sea salt added 1 liter of pure water;
8. incubate shrimp: after cleaning, with oneself sea salt through dissolving, (residue of beaker bottom discard need not), the shrimp ovum that soaked is poured in the hatching cylinder, add suitable pure water, heating rod, gas stone, temperature probe are installed, and connect the heating rod power supply: the incubation condition of shrimp is 29 ℃, continuous culture 24 hours.
9. cleaning; Used utensil cleaning is finished cleaning desktop, pond, ground;
Noon:
1. at 12:00, close the cycle water switch of the fish jar that needs feeding;
2.12:30, an amount of food is dropped in the fish jar, food is tropical fish feeding conventional food;
3. at feeding after 25 minutes, with the cycle water switch opens of closing before;
Afternoon:
1. at 16:00, close the cycle water switch of the fish jar that needs feeding;
2. ensuing operating procedure is with the operating procedure in the morning, and just asynchronism(-nization) is fed fish at 16:30; Feed behind the fish and to open cycle water in 25 minutes.
By the method for above-described embodiment 1 and embodiment 2, the zebra fish well-grown of cultivation, survival rate is up to 98% within 5 days, and the growth conditions of zebra fish is unaffected.The conventional cultural method of comparing, the soft granular bait for fish of the different bores made of feeding paramecium, fairy shrimp, eel, bait powder for example wants high through the zebra fish survival rate of the clean red worm of sterilizing etc., and the state of zebra fish is active.
Claims (6)
1. zebra fish raising method on duty for the day, this method comprises the steps:
In morning, evening:
1) before feeding fish 20-30 minute, closes the cycle water switch of the fish jar that needs feeding;
2) select corresponding shrimp hatching cylinder, gas stone is taken out from the hatching cylinder, died 5-10 minute;
3) receive shrimp: after waiting to hatch shrimp in the cylinder and sinking to the bottom, disconnect the heating rod power supply, collect shrimp with fine screen mesh, with system water shrimp is dissolved in 1 liter of beaker, stir evenly; Wait that the shrimp ovum of not hatching all sinks to the bottom, and remove the shrimp shell of water surface with fine screen mesh after;
4) feed fish: the shrimp that will remove behind the shrimp shell is poured in the wash bottle, is diluted to suitable concentration and feeds fish;
5) boiling water: after feeding 20-30 minute, with the cycle water switch opens of closing before;
6) wash cylinder; Clean hatching cylinder and heating rod with pure water;
7) claim shrimp ovum and salt: the shrimp ovum 3-5 grams per liter that scale is good, sea salt 13-16 grams per liter, shrimp ovum add pure water and soak, ventilation dissolving behind 1 liter of pure water of sea salt adding;
8) incubate shrimp: after cleaning, with oneself sea salt through dissolving, the shrimp ovum that soaked is poured in the hatching cylinder, adds suitable pure water, and heating rod, gas stone, temperature probe are installed, and connects the heating rod power supply, begins to incubate shrimp, treats to feed next time fish and uses;
9) cleaning; Used utensil cleaning is finished cleaning desktop, pond, ground;
At noon:
1) before feeding fish 20-30 minute, closes the cycle water switch of the fish jar that needs feeding;
2) an amount of food is dropped in the fish jar;
3) after feeding 20-30 minute, with the cycle water switch opens of closing before;
2. method according to claim 1, wherein zebra fish need feed morning, noon and afternoon three times every day, and the time is respectively: 8:30,12:30,16:30.
3. method according to claim 1, the incubation condition of its Prawn is 29 ℃, continuous culture 24 hours.
4. method according to claim 1 before its Prawn ovum will be used up, be taken out the canned shrimp ovum in the refrigerator in advance, uses after room temperature is placed 12 hours;
5. method according to claim 1, after wherein feeding fish and finishing 20-30 minute, open the cycle water switch after, check the water inlet pipe in the fish jar, water pipe should be more than liquid level at least 1 centimeters, current are advisable with the maximum stream flow that does not produce bubble.
6. method according to claim 1, its Prawn ovum 3 or 5 grams per liters, sea salt 13 or 16 grams per liters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102370273A CN102715116B (en) | 2012-07-10 | 2012-07-10 | Zebra fish breeding method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012102370273A CN102715116B (en) | 2012-07-10 | 2012-07-10 | Zebra fish breeding method |
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| CN102715116A CN102715116A (en) | 2012-10-10 |
| CN102715116B true CN102715116B (en) | 2013-08-07 |
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| CN109089970A (en) * | 2018-06-25 | 2018-12-28 | 扬州大学 | A kind of improved shrimps hatching and collection method |
| CN109220912B (en) * | 2018-09-28 | 2021-09-17 | 广东工业大学 | Zebra fish breed conservation culture method |
| CN110063275B (en) * | 2019-03-04 | 2020-05-22 | 中国环境科学研究院 | Zebra fish staged culture method |
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| US7503283B2 (en) * | 2004-10-24 | 2009-03-17 | Nathaniel Abraham | Aquatic egg collection research system and related devices for implementing the system |
| CN101213950B (en) * | 2008-01-16 | 2011-10-26 | 淮阴师范学院 | Procambarus clarki ovum in-vitro breeding technique |
| CN101720684B (en) * | 2008-11-03 | 2012-02-08 | 中国水产科学研究院淡水渔业研究中心 | Method for in vitro incubation of macrobrachium nipponense |
| CN201499527U (en) * | 2009-06-12 | 2010-06-09 | 北京爱生科技发展有限公司 | Multi-functional water purifying box in zebra fish and model animal breeding and development system |
| CN201467813U (en) * | 2009-06-12 | 2010-05-19 | 北京爱生科技发展有限公司 | Cultivation and fish roe collection box used in zebrafish and model animal cultivation and development system |
| CN101904311B (en) * | 2010-08-10 | 2012-10-17 | 广西壮族自治区水产研究所 | Method and device for hatching prawn larva |
| CN102217567A (en) * | 2011-06-01 | 2011-10-19 | 中国水产科学研究院淡水渔业研究中心 | In-vitro culture technology for fertilized ova of beautiful white shrimps |
| CN102283153B (en) * | 2011-07-14 | 2012-11-21 | 中国水产科学研究院淡水渔业研究中心 | Batch production fry rearing technique for exopalaemon modestus |
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