CN101213950B - Procambarus clarki ovum in-vitro breeding technique - Google Patents
Procambarus clarki ovum in-vitro breeding technique Download PDFInfo
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- CN101213950B CN101213950B CN2008100192345A CN200810019234A CN101213950B CN 101213950 B CN101213950 B CN 101213950B CN 2008100192345 A CN2008100192345 A CN 2008100192345A CN 200810019234 A CN200810019234 A CN 200810019234A CN 101213950 B CN101213950 B CN 101213950B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention put forwards an excised cultivation technology of procambarus clakii eggs according to developmental biology principle. The key points of the technology are the separation between parent prawns and eggs of berried prawns, the classification of the eggs, and respectively artificial hatching of the eggs. The steps of the invention are as follows: firstly, the berried prawns are sterilized; secondly, the parent prawns and the eggs are separated; thirdly, the eggs in different development stages are classified; fourthly, nidation of the eggs; fifthly, the hatching of the eggs. The invention has a high egg hatching rate, uniform sized young hatching prawns and a high commodity value. The cultivation technology can effectively prevent the young prawns from eating each other; therefore the survival rate of the young prawns is improved. Because the berried prawns do not need to be reared, cultivation cost is reduced and the transportation of prawn eggs is more convenient.
Description
Technical field
The present invention relates to aquaculture technology, particularly relate to the Procambarus clarki ovum in-vitro breeding method.
Background technology
(formal name used at school claims " Ke Shi crayfish " again to Procambius clarkii, is commonly called as " lobster ", " cray ", hereinafter to be referred as " lobster ".) lobster, since being made into ticbit by the people's cooking, the demand in market is increasing.Particularly in the last few years, wild lobster was owing to caught in a large number, and resource is petered out, and had become the key factor of restriction lobster industry development.In order to address the above problem, the aquatic science and technology personnel study the lobster artificial propagation techniques, and cultivation, the shrimp son who comprises the shrimp seedling in interior survives growth and become shrimp to breed or the like the aspect content.At present, the breeding method of shrimp seedling is the wild shrimp with ovums that will gather, throws in the wild environment of simulation, treat the hatching of shrimp ovum after, closeer shrimp and young shrimp are separated, separately young shrimp is cultured then.Find in the practice that this cultural technique exists some defectives, as: because shrimp ovum brooding time is asynchronous, it is asynchronous to make that young shrimp grows, thereby causes young shrimp specification uneven, has influenced the commodity price of shrimp seedling; Because lobster has the nature that kill and devour mutually, can reduce the survival rate of young shrimp again.
Summary of the invention
The present invention is according to Developmental Biology principle and the applicant achievement in research to the Procambius clarkii embryo development procedure, a kind of Procambarus clarki ovum in-vitro breeding method has been proposed, purpose is to overcome the weak point on the existing lobster artificial culture technology, to improve the incubation rate of shrimp ovum.
Technical solution of the present invention:
Key technology of the present invention is that shrimp with ovums is implemented separating of close shrimp and ovum, hatches respectively according to the residing developmental stage of shrimp ovum.The step of the inventive method is:
(1) to the shrimp with ovums disinfection: shrimp with ovums is placed in the thimerosal soaked 10-30 minute; Or be placed in the aseptic river, clean, soaked 10-20 minute;
(2) implementing close shrimp separates with ovum: separate the shrimp ovum from close shrimp belly lightly with aseptic nipper;
(3) according to the residing different developmental phases of shrimp ovum, the prawn ovum carries out classification;
(4) make the shrimp egg implantation: the shrimp ovum at the same level that will exsomatize is put down gently on the aseptic substrate seedbed in sterile chamber, slowly pours into container, submergence shrimp ovum with aseptic river;
(5) artificial incubation shrimp ovum; Enforcement is to the regulation and control of envirment factor, continuation is soaked the shrimp ovum with aseptic river, soaking depth 1-100cm, the acid-base value of adjusting river at any time is neutrality, pH value 6.8-8.2, water temperature is controlled at 15 ℃-28 ℃, illumination every day 6-12 hour, intensity of illumination 100-4000Lux (lux) is through 10-15 after day, the shrimp ovum is just hatched into young shrimp, and the incubation rate of shrimp ovum is 40%-75%.
Beneficial effect of the present invention:
(1) the incubation rate height of shrimp ovum of the present invention;
(2) the young shrimp neat specification that hatches of the present invention, the commodity value height; And can prevent from effectively to kill and devour phenomenon between young shrimp mutually, also improved the survival rate of young shrimp;
(3) the present invention compared with prior art, has reduced the cost of propagating lobster artificially because need not to feed shrimp with ovums;
(4) transportation of prawn ovum of the present invention is more more convenient than shrimp with ovums, even can carry, and does not have the restriction of traffic condition.
Embodiment
Embodiment one: shrimp with ovums is placed in the Eusol of 0.5mg/L and soaked 10 minutes, take the shrimp ovum of shrimp with ovums belly gently with aseptic nipper, the shrimp ovum that will be in the same developmental stage is placed on the seedbed, river submergence shrimp ovum with pH value 6.8 through sterilizing, submergence is 5cm, and river temperature is 15 degree, intensity of illumination 1000Lux, illumination 6 hours, cultivated through 10 days, the shrimp egg hatching rate is 41.2%.
Embodiment two: shrimp with ovums is placed in the Eusol of 0.5mg/L and soaked 15 minutes, take the shrimp ovum of shrimp with ovums belly gently with aseptic nipper, the shrimp ovum that will be in the same developmental stage is placed on the seedbed, river submergence shrimp ovum with pH value 8.2 through sterilizing, submergence is 50cm, and river temperature is 20 degree, intensity of illumination 500Lux illumination 8 hours, cultivated through 10 days, the shrimp egg hatching rate is 53%.
Embodiment three: shrimp with ovums is placed in the Eusol of 0.5mg/L and soaked 30 minutes, take the shrimp ovum of shrimp with ovums belly gently with aseptic nipper, the shrimp ovum that will be in the same developmental stage is placed on the seedbed, river submergence shrimp ovum with pH value 7.2 through sterilizing, submergence is the 100cm place, and river temperature is 25 degree, intensity of illumination 4000Lux illumination 12 hours, cultivated through 10 days, the shrimp egg hatching rate is 67.2%.
Embodiment four: shrimp with ovums is placed in the Eusol of 0.5mg/L and soaked 25 minutes, take the shrimp ovum of shrimp with ovums belly gently with aseptic nipper, the shrimp ovum that will be in the same developmental stage is placed on the seedbed, with the river submergence shrimp ovum of the pH value 7.5 through sterilizing, submergence is 30cm, and river temperature is 22 degree, intensity of illumination 2000Lux, illumination 8 hours was cultivated through 12 days, and the shrimp egg hatching rate is 74.2%.
The technology of the present invention makes full use of natural conditions and artificial regulatory combines, and has brought up the envirment factor that the shrimp ovum is hatched into the shrimp son, can bring up to more than 80% at the incubation rate of 9-10 month shrimp ovum.
Claims (3)
1. Procambarus clarki ovum in-vitro breeding method is characterized in that: this method is implemented separating of close shrimp and oophyte to shrimp with ovums, carries out the hatching of ovum more separately, the steps include:
(1) to the shrimp with ovums disinfection;
(2) implementing close shrimp separates with oophyte;
(3) the prawn ovum carries out classification;
(4) make the shrimp egg implantation;
(5) hatching shrimp ovum;
It is described that disinfection is shrimp with ovums to be placed in the thimerosal soaked 10-30 minute to shrimp with ovums; Or be placed in the aseptic river, clean, soaked 10-20 minute;
The close shrimp of described enforcement separates with oophyte, is to peel off the shrimp ovum from close shrimp belly lightly with aseptic nipper;
Described prawn ovum carries out classification, is different shrimp ovum of developmental stage is carried out classification;
The described shrimp egg implantation that makes is that the same level shrimp ovum under peeling off is put down gently on the aseptic substrate seedbed in sterile chamber, slowly pours into container, submergence shrimp ovum with aseptic river;
Described hatching shrimp ovum is to continue to soak the shrimp ovum with aseptic river, soaking depth 1-100cm, and the acid-base value of adjusting river is neutral, pH value 6.8-8.2, water temperature is controlled at 15 ℃-28 ℃, illumination every day 6-12 hour, intensity of illumination 100-4000Lux, after day, the shrimp ovum is just hatched into young shrimp through 10-15.
2. Procambarus clarki ovum in-vitro breeding method according to claim 1 is characterized in that: described disinfection is shrimp with ovums to be placed in the thimerosal soaked 15-20 minute to shrimp with ovums; Or be placed in the aseptic river, clean, soaked 15-20 minute;
Described continuation is soaked the shrimp ovum with aseptic river, soaking depth 10-50cm, and pH value 7.2-7.8,
Water temperature is at 18-25 ℃, and illumination 8-10 hour, intensity of illumination 500-2000Lux, after day, the shrimp ovum is just hatched into young shrimp through 10-15.
3. Procambarus clarki ovum in-vitro breeding method according to claim 1 is characterized in that: described disinfection is shrimp with ovums to be placed in the thimerosal soaked 25 minutes to shrimp with ovums; Described continuation is soaked the shrimp ovum with aseptic river, soaking depth 30cm, and pH value 7.5, water temperature be at 22 ℃, illumination 8 hours, intensity of illumination 2000Lux, after day, the shrimp ovum is just hatched into young shrimp through 10-15.
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| CN2008100192345A CN101213950B (en) | 2008-01-16 | 2008-01-16 | Procambarus clarki ovum in-vitro breeding technique |
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| CN101213950A CN101213950A (en) | 2008-07-09 |
| CN101213950B true CN101213950B (en) | 2011-10-26 |
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Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101503687B (en) * | 2008-11-11 | 2010-12-08 | 山东大学 | Procambarus clarki astacidin antibacterial peptide gene, coded antibacterial peptide thereof and use |
| CN101946740B (en) * | 2010-10-14 | 2012-12-12 | 淮阴师范学院 | Single hatching method of in vitro shrimp eggs of procambarus clarkii and isolated hatching inner bed thereof |
| CN102715116B (en) * | 2012-07-10 | 2013-08-07 | 中国科学院广州生物医药与健康研究院 | Zebra fish breeding method |
| CN103314908A (en) * | 2013-06-21 | 2013-09-25 | 太仓年年有渔农业科技有限公司 | Isolated breeding technique for cherax quadricarinatus eggs |
| CN103960182B (en) * | 2014-04-28 | 2016-03-09 | 江苏省海洋水产研究所 | A kind ofly apply the method that shrimp ovum cultured in vitro sets up Exopalaemon carinicauda family |
| CN107361002A (en) * | 2017-09-21 | 2017-11-21 | 江苏盱眙满江红龙虾产业园有限公司 | A kind of Procambrus clarkii ovum in-vitro culture method |
| CN107568122A (en) * | 2017-09-21 | 2018-01-12 | 江苏盱眙满江红龙虾产业园有限公司 | A kind of Procambius clarkii embryonated egg in-vitro hatching method |
| CN108184736B (en) * | 2018-03-06 | 2021-12-21 | 中国水产科学研究院淡水渔业研究中心 | Nondestructive sampling method and application of procambarus clarkii living body |
| CN108432675B (en) * | 2018-03-06 | 2021-06-01 | 中国水产科学研究院淡水渔业研究中心 | Application of eriocheir sinensis living body nondestructive sampling method |
| CN108575839A (en) * | 2018-03-14 | 2018-09-28 | 和县明信水产养殖专业合作社 | A method of transporting juvenile crab using the compound bud bed dry method of wheat-rye grass |
| CN110326568A (en) * | 2019-08-15 | 2019-10-15 | 中国水产科学研究院淡水渔业研究中心 | A kind of Procambius clarkii early stage shrimp ovum in-vitro breeding method |
| CN111304145B (en) * | 2020-03-03 | 2022-06-10 | 天津农学院 | Method for improving in vitro culture hatchability of macrobrachium rosenbergii embryos |
| CN111758625A (en) * | 2020-06-29 | 2020-10-13 | 中国水产科学研究院淡水渔业研究中心 | Method for industrial in-vitro breeding of procambarus clarkii |
| CN111758626A (en) * | 2020-06-29 | 2020-10-13 | 中国水产科学研究院淡水渔业研究中心 | Method for controlling development of in-vitro fertilized egg embryo of procambarus clarkii |
| CN113133421A (en) * | 2021-04-30 | 2021-07-20 | 广东海兴农集团有限公司 | Method for in vitro hatching of embryos of red swamp crayfish |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101002545A (en) * | 2006-12-28 | 2007-07-25 | 江苏宝龙集团有限公司 | Industrial artificial propagation method of procambarus clarkii |
Non-Patent Citations (4)
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| JP特开2001-120110A 2001.05.08 |
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