CN110050735A - A kind of dwarf clam introduces a fine variety mating system - Google Patents

A kind of dwarf clam introduces a fine variety mating system Download PDF

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Publication number
CN110050735A
CN110050735A CN201910328714.8A CN201910328714A CN110050735A CN 110050735 A CN110050735 A CN 110050735A CN 201910328714 A CN201910328714 A CN 201910328714A CN 110050735 A CN110050735 A CN 110050735A
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China
Prior art keywords
clam
dwarf
larva
accelerating
water
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Inventor
秦贞奎
杨祖晶
王浩
黄晓婷
邢强
曾启繁
陆维
包振民
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Ocean University of China
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Ocean University of China
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

Mating system is introduced a fine variety the present invention provides dwarf clam, success realize dwarf clam at home introduce a fine variety breeding, and it can be successfully realized the control of dwarf clam breeding cycle, and period control was at two months or so, it is suitable with the most fast speed of growth of dwarf clam under field conditions (factors).The present invention realizes the quickly large batch of breeding of dwarf clam, and can guarantee the acquisition of each developmental stage material of dwarf clam.In addition, the survival rate of dwarf clam of the present invention is high, and the growth rate of larva and adult is very fast compared with the dwarf clam under natural conditions.Running through entire breeding cycle, temperature maintains always higher 23 DEG C or so, to guarantee the fast-growth development of dwarf clam;The Rapid development for reducing by 2~3 DEG C in accelerating phase temperature simultaneously to stimulate sexual gland, and temperature is unlikely to too low influence growth rate, while increasing oxygenation capacity and reducing cultivation density to promote the development of close shellfish sexual gland.

Description

A kind of dwarf clam introduces a fine variety mating system
Technical field
The invention belongs to marine organisms raising technology field, be related to a kind of dwarf clam introduces a fine variety mating system.
Background technique
Dwarf clam (Mulinia lateralis) NATURAL DISTRIBUTION belongs to soft in North America the Atlantic coast and Caribbean sea area Body animal door, curtain clam mesh, clam section are that the shellfish that dwells is buried in the small-sized bivalve of one kind.It has development breeding cycle short (2 months left sides It is right), it is small, early development shell is transparent the features such as.Crown gear animal belonging to mollusk, it is big main dynamic as on the earth three One of species group, which represent primary animal types in ocean.But corresponding terrestrial animal has mature model animal system System, such as mouse, drosophila, zebra fish, mollusk is so far without model organism;And dwarf clam is state as the potentiality of mode shellfish Recognized by the society of border.
The research of dwarf clam can trace back to the fifties in last century, however, due to lacking effective artificial breeding technique, Research object be to fish based on wild population, and it is domestic just without its NATURAL DISTRIBUTION, also there are no domestic artificial breeding dwarf The report of clam.Currently, team where inventor for the first time introduces a fine variety dwarf clam to the country from the U.S., and solve dwarf clam at home Artificial breeding becomes top priority, this is for pushing the further investigation of dwarf clam and representative entire mollusk biology to grind Study carefully meaning all with positive important, this also will be helpful to the foundation of ocean model shellfish platform, and crown gear in the world is filled up in realization Animal still lacks the blank in model organism field.
Summary of the invention
The object of the present invention is to provide a kind of artificial breeding method of dwarf clam, realize dwarf clam at home introduce a fine variety with it is numerous It educating, the present invention can also breed dwarf clam with rapid scale, each developmental stage of dwarf clam larva and bodies are obtained, thus Overcome the domestic limitation that difficulty is obtained without dwarf clam NATURAL DISTRIBUTION and material.
The artificial breeding method of dwarf clam provided by the present invention, comprises the following steps that
1) parent's accelerating:
The adult dwarf clam for selecting the long 3~5mm of shell is placed in the accelerating pond disinfected in advance and carries out gonad maturity accelerating;
Wherein parent culture density is 500 pieces/m in accelerating pond2,
20~22 DEG C of the water temperature in accelerating pond, seawater salinity 28~30 ‰, pH8.0~8.2;
During parent's accelerating, feeds mix Micro Algae three times daily, feeding volume 5~60,000/ml;Furthermore every morning 1/8 yolk is fed, is uniformly sprinkling upon in pond after stranding is broken, feeds the ocean rhodotorula of 20ppm, the EM bacterium Replacement Battalion of 5ppm at night It supports;
2) it hastens parturition hatching:
It selects the full female male parent of gonadal maturation to dry in the shade, ice block cooling dries in the shade after 1.5h through 27 DEG C of water temperatures Stimulation produces essence ovulation, carries out artificial insemination;Hatched after the completion of insemination, hatches 23~24 DEG C of water temperature, hatching density controls not Higher than 50/ml water body;
During hatching by the way of ventilation, inflated size control is just risen bubble in the water surface
3) larvae cultivation:
After hatching 14~17h, the D-shaped larva to float is collected with the screen to filtrates of 400 mesh, is placed in new by disinfection In container, 23~24 DEG C of seawater that filtering is added are cultivated;It is 3~10/ml that D-shaped deutovum, which cultivates density, and temperature is not To be more than 26 DEG C, guarantee 2/3 quantity of exchanged water daily;
In D type period, using chrysophyceae as open-mouthed bait, initial 0.1~0.2 ten thousand/ml of bait throwing in density arrives shell in development After the period of top, mixing feeds chrysophyceae, Chaetoceros muelleri, Platymonas helgolandica var, Nitzschia closterlum and salt algae;As larva sends out It educates, gradually increases daily ration, feeding quantity to 0.5~10,000/ml, while gradually decreasing ratio of the chrysophyceae in mixed algae;
4) larval metamorphosis adheres to: at 23~24 DEG C of water temperature, dwarf clam larva passes through cultivation in 10~14 days, larva face by It gradually falls off, foot and water pipe gradually appear, and part larva becomes crawling creeping from swimming, and life style is also from swimming life to burying Life of dwelling changes;Fine sand is launched at this time as attachment base, original depth 0.5cm;With development, sand gradually thickeies;
5) juvenile mollusk and adult are cultivated:
Attachment base launch 3~4 days, larva gradually completes metamorphosis, start battalion bury life of dwelling, at this time i.e. enter juvenile mollusk with Adulthood;Keep density in 20,000/m2It being cultivated hereinafter, being transferred in the circulatory system in the long 1~2mm of shell, temperature stablizes 22~ 23℃;Bait is aided with salt algae, chrysophyceae and ocean rhodotorula, is fed density 6~10 based on Micro Algae (diatom, angle hair, flat algae) It ten thousand/ml, feeds three times day.When equal filial generations dwarf clam is at body shell 3~5mm of length, the new accelerating stage can be put into.
The present invention provides the mating system of introducing a fine variety of dwarf clam, successfully realize dwarf clam at home introduce a fine variety breeding, and The control of dwarf clam breeding cycle can be successfully realized, and period control is at two months or so, with dwarf clam in natural item The most fast speed of growth under part is suitable.The present invention realizes the quickly large batch of breeding of dwarf clam, and can guarantee that dwarf clam is each The acquisition of a developmental stage material.In addition, the survival rate of dwarf clam of the present invention is high, children compared with the dwarf clam under natural conditions The growth rate of worm and adult is very fast.Running through entire breeding cycle, temperature maintains always higher 23 DEG C or so, to protect Demonstrate,prove the fast-growth development of dwarf clam;The Rapid development for reducing by 2~3 DEG C in accelerating phase temperature simultaneously to stimulate sexual gland, and Temperature is unlikely to too low influence growth rate, while increasing oxygenation capacity and reducing cultivation density to promote the hair of close shellfish sexual gland It educates;In the high temperature seawater stimulation that link of hastening parturition is dried in the shade using ice block cooling with 27 DEG C, realize that dwarf clam discharges smart ovum within 1h; Dwarf clam is promoted to be rapidly completed the development of larval stage using 24 DEG C of water temperature in the larvae cultivation stage, under the conditions of this temperature Survival rate of larvae is high, the speed of growth is fast;While in order to improve the survival rate of dwarf clam larva, in conjunction with the beach life cycle of dwarf clam Border, the salinity that the present invention cultivates seawater used maintain 28~30 ‰, are lower than Qingdao sea area normal seawater salinity 30~32 ‰;? Larval metamorphosis phase and adult cultivate the phase, and the present invention launches fine sand as what attachment base carried out dwarf clam under simulating natural condition and buries life of dwelling Environment living, increases the distortion ratio and adult survival ability of dwarf clam, while with growth and development, increasing the thickness of attachment base to protect Demonstrate,prove the smooth latent sand of dwarf clam;Since dwarf clam is with respect to other shellfish development fast speeds, bait feeding amount and type are timely Variation, especially in two nodes of the secondary shell of length and settlement and metamorphosis, is replenished in time nutrition.
Detailed description of the invention
Fig. 1: dwarf's clam adult morphologic observation figure of the long 3~5mm of shell;
Fig. 2: dwarf's clam recirculation system figure;
Fig. 3: dwarf's clam morphologic observation figure of gonadal maturation;
Fig. 4: the misty sperm figure of dwarf clam discharge white after heating of drying in the shade;
Fig. 5: granular ovum figure is discharged in dwarf clam after heating of drying in the shade;
Fig. 6: dwarf clam larva hatches figure in 15L glass jar;
Fig. 7: D type larva period, the morphologic observation figure of dwarf clam;
Fig. 8: when 18d is arrived in development, the morphologic observation figure of dwarf clam.
Specific embodiment
The present invention is described further for concrete operations by the following examples.
1 parent's accelerating of embodiment and hatching of hastening parturition
The adult for selecting 500 pieces of a length of 3~5mm of shell of dwarf's clam second generation group or so carries out artificial breeding, is chosen Dwarf's clam adult is as shown in Figure 1.
1, parent's accelerating:
The dwarf's clam individual that will be singled out is put into parent's accelerating pond, is cultivated using recirculated water, as shown in Fig. 2, or It is the common circulatory system used for aquiculture;Concrete operations are as follows:
Parent's accelerating pond: first washing (including tracheae, gas stone and Chi Sai) with fresh water for pond, then fill it up with fresh water, In addition 84 medicining liquid dipping at least six hour of 10ml, after carefully scrubbed with fresh water 2 times, then rinsed again with seawater, Finally plus 4/5 or so seawater, lead to upper oxygen.
The dispensing of attachment base (sand): dwarf clam is embedded type shellfish, in order to simulate its natural benthic environment, present invention choosing Taking fine sand is attachment base (being derived from Huang Island Golden Beach), and sand is launched before first with 60~100 mesh net filtrations, removed larger miscellaneous Matter, then medicining liquid dipping sterilizes, and then rinses multipass with fresh water, and until water quality becomes clear, sand is uniformly layered on bottom of pond, About 2cm~3cm is thick, and sand has been spread, can be by pond topped up with water.
The control of condition of water quality: cultivating seawater used and be derived from the Qingdao sea area Mai Dao sand filter water, and cultivating seawater parameter used is Depending on biological nature and living environment in conjunction with dwarf clam, dwarf clam is that beach buries the shellfish that dwells, while being distributed in low latitudes sea again Area, living environment belong to warm water less salt sea area, thus accelerating water temperature be 20~22 DEG C (slightly below 22~23 DEG C of temperature of cultivation, Not only can star gonad development, but also development will not be caused too slow because of temperature is too low), salinity used in breeding seawater is 28~30 ‰ (being lower than Qingdao sea area seawater salinity 30~32 ‰), pH8.0~8.2.
Bait feeding: dwarf clam is omnivorousness shellfish, cultivates bait used referring to shellfish culture, while increasing fermentation bait Albumen, amino acid, B family vitamin, unsaturated fatty acid are supplemented, mainly includes diatom, flat algae, chrysophyceae, Chaetoceros, red ferment Mother, EM bacterium and yolk.Specifically, feeding and cultivating Micro Algae three times for one day, based on diatom, other Micro Algaes, injected volume are mixed For 5~60,000/ml, ordinary laboratory bait cultivation density about feeds 15~20L up to million/ml;Increase at night and throws It feeding 20ppm and rhodotorula 20ppm is concentrated, 5ppm EM bacterium specifically need to also be two hours after feeding, and water sampling observes water colour, if there is Bait remnants are more, can suitably reduce, otherwise increase.It should guarantee sufficient nutrition, pay attention to observation defecation situation again, keep away Exempt from overeating and indigestion.Furthermore 1/8 yolk is fed every morning, it is broken with the stranding of 300 mesh thin,tough silk, uniformly it is sprinkling upon pond In, the nutrition such as supplement lecithin.
Change water, inhale bottom and change sand: a water is changed in pond for two days, changes water before morning bait throwing in, and quantity of exchanged water is 1/3~ 1/2, water can be sucked out with water pipe, and add seawater temperature control (preventing excessive temperature differentials) in pond before changing water every time, Accelerate the ripening later period reduction quantity of exchanged water, reduces stimulation to the greatest extent;Every morning, the excrement accumulated on sand was sucked out with water pipe;Sand two weeks Left and right is changed once, can stop changing sand in the later period of accelerating the ripening.
In the present invention, the accelerating time of dwarf clam continues 20~30 days, and dwarf's clam body shell is long up to 7~10mm, dwarf The gonad development of clam is full completely, it can be observed that apparent red in female individuals, male is observed that milky Or it is light yellow, as shown in figure 3, dwarf clam can put into the reproductive stage of next step at this time.Succeed for the first time in the present invention Ground realizes the artificial repening of dwarf clam at home, compared to the maturation of natural conditions, the present invention realize dwarf clam sexual gland at Ripe time point it is flexible controllable, and relative to the inhomogeneity developed under natural conditions, the present invention can be real by artificial regulatory The sexal maturity of existing dwarf clam scale, the batch for helping to realize smart ovum obtain.
2, it hastens parturition hatching:
Parent's selects: the gonadal maturation that selecting step 1 is accelerated the ripening, full parent dwarf clam parent, according to reality It tests and needs Selection parent quantity, pay attention to distinguishing male and female.
Hasten parturition: institute's application method of hastening parturition is the temperature-raising method that dries in the shade, and determines that the time of drying in the shade is in combination with the habit of dwarf clam 1.5h and ocean temperature used of laying eggs are 27 DEG C.Operate as follows: the dwarf's clam male and female individual that will be singled out is placed on clean dried Environment dry in the shade, ice bag is put around, covers black polybag, and close light.After general 1.5h, 27 DEG C of filtering is added Seawater after general 0.5~1h, when dwarf's clam water pipe is frequent flexible, has essence and ovum respectively on the right side of male and female dwarf clam Water pipe discharge, it is generally the case that sperm drainage can prior to oviposition.The production essence amount of dwarf clam is larger, white mist is presented, such as Fig. 4 institute Show;The first egg laying amount of dwarf's clam individual is 1 X 105~5 X 105It is a, the graininess of lightpink is presented, as shown in Figure 5.
Insemination: after smart ovum is discharged in dwarf clam, first taking ovum sample to be observed, and general form of choosing is more rounded, yolk Relatively uniform full high-quality ovum is inseminated, it is not recommended that inseminating if high-quality ovum proportion is lower than 50%, because Subsequent survival rate is relatively low.After sperm is added, first it is stirred to guarantee that sperm is evenly distributed in water.It waits after 1~2min, Microscopy is sampled, there are 3~5 sperms around a general ovum.If sperm is larger, precipitated with 500 mesh thin,tough silk Method washes ovum.In addition, as time goes by, the fertility of smart ovum is gradually reduced, completed within 2 hours as far as possible.
Hatching: being transferred to 15L 50L glass jar for fertilized eggs and cultivate after insemination, in conjunction with the habit of dwarf clam, The present invention is hatched using 24 DEG C of filtering seas, it is ensured that dwarf's clam Rapid development, general density domination is in 50/ml hereinafter, herein Between usually do not have to change water.It is particularly noteworthy that the ovum of dwarf clam is demersal egg, it should be stirred inflation during hatching and supply Oxygen, preventing from sinking to the bottom extruding influences hatching, and inflated size control is just risen bubble in the water surface, as shown in Figure 6.
In the present invention, successfully realize that dwarf clam introduces a fine variety the hatching of hastening parturition behind the country for the first time, with dwarf clam under natural conditions It compares, success rate of hastening parturition is up to 90% or more.In the present invention, dwarf clam can successfully carry out early embryonic development, and general 20 ~30min dwarf clam fertilized eggs complete the discharge of polar body, and 40~50min completes First cleavage, and rate of fertilization and cleavage rates can Up to 95% or more, 14~17h can smoothly hatch to D-shaped larva period, and hatching rate is up to 70% or more.In addition, for completing The close shellfish of breeding can continue to be put into restorative cultivation in the circulatory system, initial 3d, and bait is kept to 1/3, restore normally to urge later Ripe link can put into breeding after 20~30d again.
Embodiment 2: larvae cultivation:
Select excellent: by hatching process, second-rate larva and the fertilized eggs developed not successfully can all sink to glass jar bottom Portion.The D-shaped embryophore of dwarf clam is long to be generally less than 60um, so selecting the screen to filtrate of 400 mesh to collect the larva to float, and will It is transferred in the new glass jar by disinfection, and 24 DEG C of seawater of filtering are added, finish writing label, the whole sunk to the bottom discards.This It is outer that excellent tool is selected to need with sterilizing in advance
Larvae cultivation: select it is excellent after, into the larvae cultivation stage, larva individual is small around here, and accommodative ability of environment is poor, To water quality requirement height, it is necessary to conscientiously manage.Ingesting, giving birth to for room temperature, water temperature, the variation of water colour and larva will be observed daily Long situation, whether inflation normal and larvae development period.It cultivates density and is generally 3~10/milliliter, water temperature does not exceed 26 DEG C, salinity is 28~30 ‰, guarantees daily 2/3 quantity of exchanged water, periodically clear bottom and replacement glass jar.Dwarf clam is as omnivorousness shellfish Class, bait are opened based on Isochrysis galbana, Chaetoceros muelleri, Platymonas helgolandica var, Nitzschia closterlum, salt algae in D type period Begin, using proof gold algae as open-mouthed bait, initial 0.1~0.2 ten thousand/ml of bait throwing in density, after developing to shell top period, mixing is thrown Hello.With larvae development, daily ration, feeding quantity is gradually increased to 0.5~10,000/ml, while gradually decreasing ratio of the chrysophyceae in mixed algae Example, day throw 4 times.In addition it is also necessary to supplement, rhodotorula is concentrated in 2ppm and 2ppm EM bacterium supplements the nutrients as nutrition bait, is mentioned The survival rate of high larva.Generally on shell top, period dwarf clam has the higher death rate, uses 300 mesh screens instead at this time and is changed Water, and often to replace glass jar.With larvae development, the mesh size for changing water need to be gradually increased.
The dispensing of attachment base: under 24 DEG C of water temperature, bait sufficiency, dwarf clam larva passes through cultivation in 10~14 days, Larva face gradually falls off, and foot and water pipe gradually appear, and part larva becomes crawling creeping from swimming, and life style is also by swimming Swimming life changes to life of dwelling is buried.Need to launch fine sand at this time as attachment base, original depth 0.5cm.It is husky with development Son gradually thickeies, it is notable that sand need to be washed and be disinfected after the screening of 100 mesh screens, and removing as far as possible may Existing biology and organic dirt.
Under breeding condition provided by the present invention, dwarf clam is successful from D type larval stage (shown in Fig. 7) development and fast Speed completes the development of larval stage, and initially enters larval metamorphosis period in 10~14d, uses fine sand as attachment base. In the present invention, 30%~50% larva can smoothly pass through the survival of shell top larva period to when metamorphosis from D-shaped larva period Phase, at first 3 days of larvae development, the long speed of growth of shell was up to 5um/d;One week later, the long speed of growth of shell was reachable 15um/d;At following 5 days, the speed of growth was reduced, and is maintained at 5um/d, and shell is grown in 190~220um or so at this time, i.e., will be into Enter period of metamorphosis
2, juvenile mollusk and adult are cultivated:
Attachment base launch 3~4 days, larva gradually completes metamorphosis, start battalion bury life of dwelling, at this time i.e. enter juvenile mollusk with Adulthood.Suitable Density is kept, control is in 20,000/m2 hereinafter, density need to be reduced accordingly as juvenile mollusk is grown, generally in naked eyes It can be seen that when (shell long 1~2mm), need to reduce density or be transferred in the circulatory system cultivating, temperature is stablized at 22~23 DEG C or so. Furthermore in the circulatory system, it should be noted that adjust water flow size, water flow is excessive to rush juvenile mollusk, into the circulatory system. Bait is aided with salt algae, chrysophyceae, ocean rhodotorula and EM bacterium, is generally fed density 6 based on Micro Algae (diatom, angle hair, flat algae) It~10 ten thousand/ml, feeds three times day.In the present invention, dwarf clam can smoothly complete metamorphosis, and juvenile mollusk development and adult are raw It is long, wherein the larva 50%~70% in abnormal period can complete metamorphosis, switch to bury life of dwelling from life of swimming, in 18d When, dwarf's clam juvenile mollusk shell length can be grown to 600~800um;In 25d, 1~2mm can be grown to, as shown in Figure 8;About 30~40d When, dwarf clam adult about shell grows to 3~5mm, can be selected from offspring at this time the cooling of a certain amount of dwarf clam cultivate into Enter the breeding cycle of next round.

Claims (5)

1. a kind of artificial breeding method of dwarf clam, comprises the following steps that
1) parent's accelerating:
The adult dwarf clam for selecting the long 3~5mm of shell is placed in the accelerating pond disinfected in advance and carries out gonad maturity accelerating;
2) it hastens parturition hatching:
It selects the full female male parent of gonadal maturation to dry in the shade, ice block cooling dries in the shade after 1.5h through 27 DEG C of water temperatures stimulations Essence ovulation is produced, artificial insemination is carried out;Hatched after the completion of insemination, hatches 23~24 DEG C of water temperature, hatching density control is not higher than 50/ml water body;
3) larvae cultivation:
After hatching 14~17h, the D-shaped larva to float is collected with the screen to filtrate of 400 mesh, is placed in the new container by disinfection In, 23~24 DEG C of seawater that filtering is added are cultivated;It is 3~10/ml that D-shaped deutovum, which cultivates density, and temperature not surpass 26 DEG C are crossed, guarantees 2/3 quantity of exchanged water daily;
In D type period, using chrysophyceae as open-mouthed bait, initial 0.1~0.2 ten thousand/ml of bait throwing in density, when shell top is arrived in development After phase, mixing feeds chrysophyceae, Chaetoceros muelleri, Platymonas helgolandica var, Nitzschia closterlum and salt algae;With larvae development, by Cumulative plus daily ration, feeding quantity gradually decreases ratio of the chrysophyceae in mixed algae to 0.5~10,000/ml;
4) larval metamorphosis adheres to: at 23~24 DEG C of water temperature, dwarf clam larva passes through cultivation in 10~14 days, and larva face gradually takes off It falls, foot and water pipe gradually appear, and part larva becomes crawling creeping from swimming, and life style is also from swimming life to burying life of dwelling Transformation living;Fine sand is launched at this time as attachment base, original depth 0.5cm;With development, sand gradually thickeies;
5) juvenile mollusk and adult are cultivated:
It is launched 3~4 days in attachment base, larva gradually completes metamorphosis, starts battalion and buries life of dwelling, enters juvenile mollusk and adult at this time Stage;Keep density in 20,000/m2It is cultivated hereinafter, being transferred in the circulatory system in the long 1~2mm of shell, temperature is stablized 22~23 ℃;Bait is aided with salt algae, chrysophyceae and ocean rhodotorula based on Micro Algae, feeds density 6~100,000/ml, day feeds three It is secondary;When equal filial generations dwarf clam is at body shell 3~5mm of length, the new accelerating stage can be put into.
2. the method as described in claim 1, which is characterized in that described 1) in accelerating pond parent culture density be 500 pieces/ m2
3. the method as described in claim 1, which is characterized in that described 1) in 20~22 DEG C of water temperature of accelerating pond, seawater salt Spend 28~30 ‰, pH8.0~8.2.
4. the method as described in claim 1, which is characterized in that described 1) in during parent's accelerating, feed daily three times Mix Micro Algae, feeding volume 5~60,000/ml;Furthermore it feeds 1/8 yolk every morning, is uniformly sprinkling upon in pond after stranding is broken, evening On feed the ocean rhodotorula of 20ppm, the EM bacterium of 5ppm supplements the nutrients.
5. the method as described in claim 1, which is characterized in that described 5) in Micro Algae be diatom, angle hair or flat algae.
CN201910328714.8A 2019-04-23 2019-04-23 A kind of dwarf clam introduces a fine variety mating system Pending CN110050735A (en)

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Publication number Priority date Publication date Assignee Title
CN111557258A (en) * 2020-06-17 2020-08-21 中国海洋大学 Artificial temperature accumulation and ripening method for bay scallop breeding shells

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