CN102147416A - 稳定的缺血修饰白蛋白测定试剂盒 - Google Patents
稳定的缺血修饰白蛋白测定试剂盒 Download PDFInfo
- Publication number
- CN102147416A CN102147416A CN2011100217664A CN201110021766A CN102147416A CN 102147416 A CN102147416 A CN 102147416A CN 2011100217664 A CN2011100217664 A CN 2011100217664A CN 201110021766 A CN201110021766 A CN 201110021766A CN 102147416 A CN102147416 A CN 102147416A
- Authority
- CN
- China
- Prior art keywords
- reagent
- dtt
- dithiothreitol
- cobalt ions
- stable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010023015 ischemia-modified albumin Proteins 0.000 title abstract description 5
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims abstract description 75
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 74
- XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 claims abstract description 21
- 229910001429 cobalt ion Inorganic materials 0.000 claims abstract description 20
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 20
- 108010088751 Albumins Proteins 0.000 claims description 16
- 102000009027 Albumins Human genes 0.000 claims description 16
- 239000008213 purified water Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 12
- 208000028867 ischemia Diseases 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 230000004048 modification Effects 0.000 claims description 10
- 238000012986 modification Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 9
- 239000000470 constituent Substances 0.000 claims description 8
- 239000003381 stabilizer Substances 0.000 claims description 7
- RAXXELZNTBOGNW-UHFFFAOYSA-N 1H-imidazole Chemical compound C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims description 5
- 239000007983 Tris buffer Substances 0.000 claims description 5
- GFHNAMRJFCEERV-UHFFFAOYSA-L cobalt chloride hexahydrate Chemical compound O.O.O.O.O.O.[Cl-].[Cl-].[Co+2] GFHNAMRJFCEERV-UHFFFAOYSA-L 0.000 claims description 5
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 5
- 230000003647 oxidation Effects 0.000 abstract description 3
- 238000007254 oxidation reaction Methods 0.000 abstract description 3
- 230000002829 reductive effect Effects 0.000 abstract description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052760 oxygen Inorganic materials 0.000 abstract description 2
- 239000001301 oxygen Substances 0.000 abstract description 2
- 239000002736 nonionic surfactant Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 3
- 229910017052 cobalt Inorganic materials 0.000 description 2
- 239000010941 cobalt Substances 0.000 description 2
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 2
- 230000000302 ischemic effect Effects 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 229910052723 transition metal Inorganic materials 0.000 description 2
- 150000003624 transition metals Chemical class 0.000 description 2
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
稳定剂 | 浓度(%) | DTT剩余率(%) |
无 | 无 | 46.1 |
曲拉通 | 0.05 | 95.3 |
稳定剂 | 浓度(%) | DTT剩余率(%) |
无 | 无 | 46.1 |
聚氧化乙烯烷基酚醚 | 0.05 | 95.3 |
稳定剂 | 浓度(%) | DTT剩余率(%) |
无 | 无 | 46.1 |
十二烷基苯磺酸钠 | 0.05 | 95.3 |
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110021766.4A CN102147416B (zh) | 2011-01-19 | 2011-01-19 | 稳定的缺血修饰白蛋白测定试剂盒 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110021766.4A CN102147416B (zh) | 2011-01-19 | 2011-01-19 | 稳定的缺血修饰白蛋白测定试剂盒 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102147416A true CN102147416A (zh) | 2011-08-10 |
CN102147416B CN102147416B (zh) | 2015-04-22 |
Family
ID=44421788
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201110021766.4A Active CN102147416B (zh) | 2011-01-19 | 2011-01-19 | 稳定的缺血修饰白蛋白测定试剂盒 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102147416B (zh) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102507916A (zh) * | 2011-11-07 | 2012-06-20 | 宁波美康生物科技股份有限公司 | 缺血修饰白蛋白液体稳定试剂盒 |
CN103558397A (zh) * | 2013-11-04 | 2014-02-05 | 山东博科生物产业有限公司 | 一种缺血修饰白蛋白检测试剂及检测方法 |
CN103558398A (zh) * | 2013-11-04 | 2014-02-05 | 山东博科生物产业有限公司 | 一种抗肝素干扰的缺血修饰白蛋白检测试剂 |
CN103760357A (zh) * | 2013-11-04 | 2014-04-30 | 山东博科生物产业有限公司 | 一种缺血修饰白蛋白检测试剂盒 |
CN105021609A (zh) * | 2015-07-06 | 2015-11-04 | 广州金域医学检验中心有限公司 | 一种缺血修饰白蛋白检测试剂及其检测方法 |
CN105181692A (zh) * | 2015-09-30 | 2015-12-23 | 山东博科生物产业有限公司 | 一种缺血修饰白蛋白比色法检测试剂盒 |
CN106093017A (zh) * | 2016-04-28 | 2016-11-09 | 安徽伊普诺康生物技术股份有限公司 | 一种测定缺血修饰白蛋白的试剂盒及其制备方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1888907A (zh) * | 2006-08-03 | 2007-01-03 | 北京航天总医院 | 一种缺血修饰白蛋白的测定方法 |
CN1959377A (zh) * | 2006-05-31 | 2007-05-09 | 长沙颐康科技开发有限公司 | 缺血修饰白蛋白组合测定试剂及其使用方法 |
EP1857818A1 (en) * | 2006-05-15 | 2007-11-21 | DIGILAB BioVisioN GmbH | Diagnostic and therapeutic uses of peptides for pre-forms of type 2 diabetes and conditions associated therewith |
CN101458257A (zh) * | 2007-12-12 | 2009-06-17 | 上海复星医药(集团)股份有限公司 | 检测缺血修饰白蛋白的试剂盒及其制备方法 |
US20090220919A1 (en) * | 2008-02-28 | 2009-09-03 | Jen-Chang Yang | Dental Bleaching Gel Composition Containing Vegetative Enzymes |
-
2011
- 2011-01-19 CN CN201110021766.4A patent/CN102147416B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1857818A1 (en) * | 2006-05-15 | 2007-11-21 | DIGILAB BioVisioN GmbH | Diagnostic and therapeutic uses of peptides for pre-forms of type 2 diabetes and conditions associated therewith |
CN1959377A (zh) * | 2006-05-31 | 2007-05-09 | 长沙颐康科技开发有限公司 | 缺血修饰白蛋白组合测定试剂及其使用方法 |
CN1888907A (zh) * | 2006-08-03 | 2007-01-03 | 北京航天总医院 | 一种缺血修饰白蛋白的测定方法 |
CN101458257A (zh) * | 2007-12-12 | 2009-06-17 | 上海复星医药(集团)股份有限公司 | 检测缺血修饰白蛋白的试剂盒及其制备方法 |
US20090220919A1 (en) * | 2008-02-28 | 2009-09-03 | Jen-Chang Yang | Dental Bleaching Gel Composition Containing Vegetative Enzymes |
Non-Patent Citations (2)
Title |
---|
孙金芳: "缺血修饰白蛋白的检测及临床应用", 《医学综述》 * |
陈志晓等: "改良缺血修饰白蛋白测定方法(白蛋白-钴结合试验)的建立", 《检验医学》 * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102507916A (zh) * | 2011-11-07 | 2012-06-20 | 宁波美康生物科技股份有限公司 | 缺血修饰白蛋白液体稳定试剂盒 |
CN102507916B (zh) * | 2011-11-07 | 2013-11-06 | 宁波美康生物科技股份有限公司 | 缺血修饰白蛋白液体稳定试剂盒 |
CN103558397A (zh) * | 2013-11-04 | 2014-02-05 | 山东博科生物产业有限公司 | 一种缺血修饰白蛋白检测试剂及检测方法 |
CN103558398A (zh) * | 2013-11-04 | 2014-02-05 | 山东博科生物产业有限公司 | 一种抗肝素干扰的缺血修饰白蛋白检测试剂 |
CN103760357A (zh) * | 2013-11-04 | 2014-04-30 | 山东博科生物产业有限公司 | 一种缺血修饰白蛋白检测试剂盒 |
CN103558397B (zh) * | 2013-11-04 | 2015-06-10 | 山东博科生物产业有限公司 | 一种缺血修饰白蛋白检测试剂及检测方法 |
CN103760357B (zh) * | 2013-11-04 | 2016-02-03 | 山东博科生物产业有限公司 | 一种缺血修饰白蛋白检测试剂盒 |
CN105021609A (zh) * | 2015-07-06 | 2015-11-04 | 广州金域医学检验中心有限公司 | 一种缺血修饰白蛋白检测试剂及其检测方法 |
CN105181692A (zh) * | 2015-09-30 | 2015-12-23 | 山东博科生物产业有限公司 | 一种缺血修饰白蛋白比色法检测试剂盒 |
CN106093017A (zh) * | 2016-04-28 | 2016-11-09 | 安徽伊普诺康生物技术股份有限公司 | 一种测定缺血修饰白蛋白的试剂盒及其制备方法 |
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CN102147416B (zh) | 2015-04-22 |
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Address after: 610000 No. 101 Tian Xin Road, hi tech Zone, Sichuan, Chengdu Applicant after: SICHUAN XINJIAN KANGCHENG BIOLOGICAL CO., LTD. Address before: 610000 Sichuan province Chengdu city Chengdu high tech Zone Tianchen Road No. 88 Applicant before: Sichuan Province Xincheng Biological Technology Co., Ltd. |
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Free format text: CORRECT: APPLICANT; FROM: SICHUAN XINCHENG BIOTECHNOLOGY CO., LTD. TO: SICHUAN XINJIANKANGCHENG BIOLOG TECHNOLOGY CO., LTD. |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Stable ischemia modified albumin testing kit Effective date of registration: 20161214 Granted publication date: 20150422 Pledgee: Chengdu technology transfer (Group) Co., Ltd. Pledgor: SICHUAN XINJIAN KANGCHENG BIOLOGICAL CO., LTD. Registration number: 2016990001096 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20171222 Granted publication date: 20150422 Pledgee: Chengdu technology transfer (Group) Co., Ltd. Pledgor: SICHUAN XINJIAN KANGCHENG BIOLOGICAL CO., LTD. Registration number: 2016990001096 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right |