CN102089420B - 改进的气体可渗透性细胞培养装置及使用方法 - Google Patents

改进的气体可渗透性细胞培养装置及使用方法 Download PDF

Info

Publication number
CN102089420B
CN102089420B CN200980126878.7A CN200980126878A CN102089420B CN 102089420 B CN102089420 B CN 102089420B CN 200980126878 A CN200980126878 A CN 200980126878A CN 102089420 B CN102089420 B CN 102089420B
Authority
CN
China
Prior art keywords
gas
compartment
gas compartment
opening
support
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN200980126878.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN102089420A (zh
Inventor
约翰·R·威尔逊
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wilson Wolf Manufacturing LLC
Original Assignee
Wilson Wolf Manufacturing LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wilson Wolf Manufacturing LLC filed Critical Wilson Wolf Manufacturing LLC
Publication of CN102089420A publication Critical patent/CN102089420A/zh
Application granted granted Critical
Publication of CN102089420B publication Critical patent/CN102089420B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/24Gas permeable parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/40Manifolds; Distribution pieces

Landscapes

  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN200980126878.7A 2008-07-08 2009-07-08 改进的气体可渗透性细胞培养装置及使用方法 Active CN102089420B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7896608P 2008-07-08 2008-07-08
US61/078,966 2008-07-08
PCT/US2009/049944 WO2010006055A2 (en) 2008-07-08 2009-07-08 Improved gas permeable cell culture device and method of use

Publications (2)

Publication Number Publication Date
CN102089420A CN102089420A (zh) 2011-06-08
CN102089420B true CN102089420B (zh) 2014-08-13

Family

ID=41507707

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200980126878.7A Active CN102089420B (zh) 2008-07-08 2009-07-08 改进的气体可渗透性细胞培养装置及使用方法

Country Status (7)

Country Link
US (1) US8518692B2 (enExample)
EP (1) EP2297295A4 (enExample)
JP (1) JP5395171B2 (enExample)
CN (1) CN102089420B (enExample)
AU (1) AU2009268666B2 (enExample)
CA (1) CA2730104A1 (enExample)
WO (1) WO2010006055A2 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007511205A (ja) 2003-10-08 2007-05-10 ウィルソン ウォルフ マニュファクチャリング コーポレイション 気体透過性物質を利用する細胞を培養する方法及び装置
US7745209B2 (en) 2005-07-26 2010-06-29 Corning Incorporated Multilayered cell culture apparatus
CN101611133A (zh) * 2006-12-07 2009-12-23 威尔森沃尔夫制造公司 高效装置及培养细胞的方法
US8518692B2 (en) 2008-07-08 2013-08-27 Wilson Wolf Manufacturing Corporation Gas permeable cell culture device and method of use
US8778669B2 (en) 2009-07-22 2014-07-15 Corning Incorporated Multilayer tissue culture vessel
US9005550B2 (en) 2012-10-29 2015-04-14 Corning Incorporated Multi-layered cell culture vessel with manifold grips
US9790465B2 (en) 2013-04-30 2017-10-17 Corning Incorporated Spheroid cell culture well article and methods thereof
US10260038B2 (en) 2013-05-10 2019-04-16 Whitehead Institute For Biomedical Research Protein modification of living cells using sortase
KR102470508B1 (ko) * 2014-10-29 2022-11-24 코닝 인코포레이티드 관류 생물반응기 플랫폼
KR102460969B1 (ko) 2014-10-29 2022-10-31 코닝 인코포레이티드 세포 배양 인서트
EP3212761A1 (en) 2014-10-29 2017-09-06 Corning Incorporated Microwell design and fabrication for generation of cell culture aggregates
WO2016103485A1 (ja) * 2014-12-26 2016-06-30 株式会社サンプラテック 培養フラスコ
CN105062889B (zh) * 2015-07-30 2017-11-17 哈尔滨医科大学 一种细胞挥发物收集培养器
JP2018033387A (ja) * 2016-08-31 2018-03-08 株式会社アステック 細胞培養容器
JP2018143210A (ja) * 2017-03-08 2018-09-20 株式会社アステック 細胞培養容器
WO2018195014A1 (en) 2017-04-19 2018-10-25 Neubiser Richard Bioreactor for biological material
US11857970B2 (en) 2017-07-14 2024-01-02 Corning Incorporated Cell culture vessel
JP7195302B2 (ja) 2017-07-14 2022-12-23 コーニング インコーポレイテッド 3d培養のための細胞培養容器及び3d細胞の培養方法
CN111051494B (zh) 2017-07-14 2024-03-29 康宁股份有限公司 用于手动或自动培养基交换的3d细胞培养容器
JP7171696B2 (ja) 2018-07-13 2022-11-15 コーニング インコーポレイテッド 相互接続されたウェルを有するマイクロプレートを備えた流体デバイス
PL3649229T3 (pl) 2018-07-13 2021-12-06 Corning Incorporated Naczynia do hodowli komórkowych ze stabilizującymi urządzeniami
CN111032851B (zh) 2018-07-13 2024-03-29 康宁股份有限公司 具有包含液体介质传递表面的侧壁的微腔皿
WO2021081170A1 (en) * 2019-10-24 2021-04-29 Octane Biotech Inc. Cell culture chamber with improved cell-contacting surfaces
KR102417445B1 (ko) * 2019-11-29 2022-07-06 주식회사 아모그린텍 세포배양장치

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693537A (en) * 1994-06-28 1997-12-02 Wilson; John R. Compartmentalized tissue culture flask
US6562616B1 (en) * 1999-06-21 2003-05-13 The General Hospital Corporation Methods and devices for cell culturing and organ assist systems
US20050148068A1 (en) * 2003-12-31 2005-07-07 Lacey William J. Cell cultivating flask
US20070026516A1 (en) * 2005-07-26 2007-02-01 Martin Gregory R Multilayered cell culture apparatus
WO2008073314A2 (en) * 2006-12-07 2008-06-19 Wilson Wolf Manufacturing Corporation Highly efficient devices and methods for culturing cells

Family Cites Families (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3459176A (en) 1966-06-24 1969-08-05 Beckman Instruments Inc Apparatus and method of sampling a dialyzable component of blood
GB1376131A (en) 1971-02-09 1974-12-04 Nat Res Dev Improvements in and relating to cell culture systems
DE2128744C3 (de) 1971-06-09 1979-03-29 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V., 3400 Goettingen Verfahren zur Massenkultivation von Zellen und Geweben
US3839155A (en) 1972-07-27 1974-10-01 Merck & Co Inc Cell and vaccine production
US3870602A (en) 1973-04-30 1975-03-11 California Lab Ind Inc Gas permeable sterile culture bottle
US3941661A (en) 1974-01-16 1976-03-02 The Curators Of The University Of Missouri Roller culture bottle insert
US4228243A (en) 1978-07-13 1980-10-14 Toray Industries, Inc. Cell culture propagation apparatus
US4296205A (en) 1980-02-22 1981-10-20 Verma Dharmvir S Cell culture and continuous dialysis flask and method
US4317886A (en) 1980-08-11 1982-03-02 Becton, Dickinson And Company Multiple interior surface roller bottle
US4435508A (en) 1981-11-20 1984-03-06 Gabridge Michael G Tissue culture vessel
DE3409501A1 (de) 1984-03-15 1985-10-24 Sandoz-Patent-GmbH, 7850 Lörrach Verfahren zur kultivierung von zellen
US4748124A (en) 1984-10-30 1988-05-31 E. I. Du Pont De Nemours And Company Compartmentalized cell-culture device and method
US4668632A (en) 1985-02-06 1987-05-26 Vxr, Inc. Sparger and apparatus for and method of growing cells
US4654308A (en) 1985-06-19 1987-03-31 La Corporation De L'ecole Polytechnique Bioreactor
US4661455A (en) 1986-01-16 1987-04-28 Dorr-Oliver Incorporated Membrane cell culturing device
US4937194A (en) 1986-05-12 1990-06-26 Baxter International Inc. Method for metering nutrient media to cell culture containers
US4829002A (en) 1986-05-12 1989-05-09 Baxter International Inc. System for metering nutrient media to cell culture containers and method
US4734373A (en) 1986-06-24 1988-03-29 Bartal Arie H Apparatus for enhancing cell growth, preservation and transport
US4847462A (en) 1986-11-06 1989-07-11 American Fluoroseal Corporation Method and apparatus for making fluorocarbon film plastic bags using a laser
US4945203A (en) 1986-11-06 1990-07-31 American Fluoroseal Corporation Method and apparatus for making fluorocarbon film plastic bags using a laser
US4717668A (en) 1986-12-12 1988-01-05 Baxter Travenol Laboratories, Inc. Plastic roller bottle for suspension cultures
US5047347A (en) 1987-08-17 1991-09-10 Cline Martin J Gas permeable culture flask and method for culturing mammalian cells
US4839292B1 (en) 1987-09-11 1994-09-13 Joseph G Cremonese Cell culture flask utilizing membrane barrier
US4829004A (en) 1987-11-24 1989-05-09 The University Of Michigan Roller bottle system
US4824787A (en) 1987-12-09 1989-04-25 In Vitro Scientific Products, Inc. Roller bottle for tissue culture growth
US5153131A (en) 1990-12-11 1992-10-06 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration High aspect reactor vessel and method of use
US5026650A (en) 1988-06-30 1991-06-25 The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration Horizontally rotated cell culture system with a coaxial tubular oxygenator
CA1307225C (en) 1988-07-19 1992-09-08 David W. Armstrong Cell culture bioreactor
DE68926421T2 (de) 1988-08-20 1996-09-12 Nitto Denko Corp Verfahren zur Entfernung von gelösten Gasen aus einer Flüssigkeit
US4939151A (en) 1988-10-31 1990-07-03 Baxter International Inc. Adherent cell culture flask
US5068195A (en) 1988-11-02 1991-11-26 Du Pont Merck Pharmaceutical Company Dot matrix membrane cell expansion and maintenance vessel
US4960706A (en) 1989-03-27 1990-10-02 Baxter International, Inc. Static oxygenator for suspension culture of animal cells
US4912058A (en) 1989-04-27 1990-03-27 Becton, Dickinson And Company Roller bottle
US5240854A (en) 1989-06-05 1993-08-31 Berry Eric S Continuous high-density cell culture system
US4937196A (en) 1989-08-18 1990-06-26 Brunswick Corporation Membrane bioreactor system
US5173225A (en) 1990-05-07 1992-12-22 Bowolfe, Inc. Method for making ultra-thin dense skin membrane
EP0471947A1 (en) 1990-06-29 1992-02-26 Sekisui Chemical Co., Ltd. Culture bag
FR2666094A1 (fr) 1990-08-22 1992-02-28 Cyclopore Sa Bouteille de culture en masse de cellules et procede pour son assemblage.
US5139951A (en) 1990-10-10 1992-08-18 Costar Corporation Culture device having a detachable cell or tissue growth surface
US5650325A (en) 1990-11-29 1997-07-22 Spielmann; Richard Apparatus having a rotatable stack of parallel trays with culturing surfaces on opposite sides for liquid/gas exchange
CA2055966C (en) 1990-12-19 1995-08-01 Oresta Natalia Fedun Cell culture insert
CA2062100C (en) 1991-03-05 2000-10-31 Hirotoshi Fujisawa Disc cartridge
GB9112836D0 (en) 1991-06-14 1991-07-31 Medical Res Council Production of monoclonal antibodies
US5310676A (en) 1991-11-15 1994-05-10 A/S Nunc Cell cultivating device
DE4214157C1 (enExample) 1992-04-29 1993-08-05 Degussa Ag, 6000 Frankfurt, De
GB2268187A (en) 1992-07-01 1994-01-05 Univ Hull Cell culture vessels
DE4229325C2 (de) 1992-09-02 1995-12-21 Heraeus Instr Gmbh Kulturgefäß für Zellkulturen
DE4229334C2 (de) 1992-09-02 1995-12-21 Heraeus Instr Gmbh Rollflaschenähnliches Kulturgefäß für Zellkulturen
US5330908A (en) 1992-12-23 1994-07-19 The United States Of America As Represented By The Administrator, National Aeronautics And Space Administration High density cell culture system
US5324428A (en) 1993-07-19 1994-06-28 Spectrum Medical Industries, Inc. Disposable dialysis apparatus
AT401476B (de) 1993-08-20 1996-09-25 Sy Lab Vertriebsges M B H Thermokammer
US5686301A (en) 1993-09-02 1997-11-11 Heraeus Instruments Gmbh Culture vessel for cell cultures
US5702941A (en) 1993-09-09 1997-12-30 Synthecon, Inc. Gas permeable bioreactor and method of use
US5437998A (en) 1993-09-09 1995-08-01 Synthecon, Inc. Gas permeable bioreactor and method of use
WO1995008385A1 (en) 1993-09-22 1995-03-30 Pierce Chemical Company Device and method for dialyzing samples
FR2710492B1 (fr) 1993-09-29 1997-08-01 Compac Int Inc Sac pour la culture de blanc de champignon.
US5534423A (en) 1993-10-08 1996-07-09 Regents Of The University Of Michigan Methods of increasing rates of infection by directing motion of vectors
DE69513114T2 (de) 1994-02-10 2000-04-13 Kao Corp., Tokio/Tokyo Verfahren zur herstellung von acyloxybenzolsulfonsäure oder einem salz davon
US5707869A (en) 1994-06-28 1998-01-13 Wolf; Martin L. Compartmentalized multiple well tissue culture plate
US5714384A (en) 1994-06-28 1998-02-03 Wilson; John R. Compartmentalized tissue culture bag
US5527705A (en) 1994-09-12 1996-06-18 Becton, Dickinson And Company Roller bottle for trans-membrane co-culture of cells and method for its use
US5935847A (en) 1994-10-28 1999-08-10 Baxter International Inc. Multilayer gas-permeable container for the culture of adherent and non-adherent cells
US6297046B1 (en) 1994-10-28 2001-10-02 Baxter International Inc. Multilayer gas-permeable container for the culture of adherent and non-adherent cells
DE19504958C1 (de) 1995-02-15 1995-11-02 Heraeus Instr Gmbh Verfahren für die Kultivierung von Zellen auf einem Träger, Vorrichtung zur Durchführung des Verfahrens und Verwendung der Vorrichtung
BE1009306A5 (fr) 1995-04-28 1997-02-04 Baxter Int Bioreacteur.
CA2175626C (en) 1995-05-18 1999-08-03 Timothy A. Stevens Tissue culture flask
US5985653A (en) 1995-06-07 1999-11-16 Aastrom Biosciences, Inc. Incubator apparatus for use in a system for maintaining and growing biological cells
DE19529099C2 (de) 1995-08-08 1997-12-04 Forschungszentrum Juelich Gmbh Vorrichtung zur Serien-Kultivierung von Mikroorganismen bzw. Zellen in begasten Flüssigkeitssäulen
DE69612861T2 (de) 1995-08-31 2001-08-30 Ashby Scientific Ltd., Coalville Verfahren und vorrichtung zur kultivierung von biologischem material
US5686304A (en) 1995-12-15 1997-11-11 Avecor Cardiovascular, Inc. Cell culture apparatus and method
US5866419A (en) 1996-09-16 1999-02-02 Meder; Martin G. Roller bottle
US5876604A (en) 1996-10-24 1999-03-02 Compact Membrane Systems, Inc Method of gasifying or degasifying a liquid
US6306491B1 (en) 1996-12-20 2001-10-23 Gore Enterprise Holdings, Inc. Respiratory aids
US5817509A (en) * 1997-03-19 1998-10-06 Becton Dickinson And Company Culture vessel assembly
US5928936A (en) 1997-04-09 1999-07-27 Huntington Medical Research Institutes Cell culture container that self-seals after cannula penetration made of porous sheets
DE19719751A1 (de) 1997-05-10 1998-11-12 Augustinus Dr Med Bader Vorrichtung zum Züchten und/oder Behandeln von Zellen
US5914154A (en) 1997-05-30 1999-06-22 Compact Membrane Systems, Inc. Non-porous gas permeable membrane
US5783075A (en) 1997-06-23 1998-07-21 Spectrum Medical Laboratories, Inc. Disposable dialyzer apparatus
JP3761676B2 (ja) 1997-07-11 2006-03-29 株式会社メニコン 細胞培養用容器
US6190913B1 (en) 1997-08-12 2001-02-20 Vijay Singh Method for culturing cells using wave-induced agitation
US6596532B1 (en) 1997-12-12 2003-07-22 BIOMéRIEUX, INC. Device for isolation and surface culture of microorganisms from bulk fluids
GB9803362D0 (en) 1998-02-17 1998-04-15 Cellon Sa A cell culture vessel
US5989913A (en) 1998-07-02 1999-11-23 Charles Daniel Anderson Culture vessel for growing or culturing cells, cellular aggregates, tissues and organoids and methods for using the same
CA2348493C (en) 1998-10-28 2010-08-24 Ashby Scientific Limited Textured and porous silicone rubber
GB9912641D0 (en) 1999-05-28 1999-07-28 Ashby Scient Ltd Textured and porous silicone rubber
US6410309B1 (en) 1999-03-23 2002-06-25 Biocrystal Ltd Cell culture apparatus and methods of use
US6455310B1 (en) 1999-03-23 2002-09-24 Biocrystal Ltd. Cell culture apparatus and method for culturing cells
DE19915611A1 (de) 1999-04-07 2000-11-16 Augustinus Bader Rollhalterung
US7560274B1 (en) 1999-05-28 2009-07-14 Cellon S.A. Culture chamber
EP1203075B1 (en) 1999-06-21 2007-09-12 The General Hospital Corporation Cell culture systems and methods for organ assist devices
US6569675B2 (en) 2000-06-16 2003-05-27 Corning Incorporated Cell cultivating flask and method for using the cell cultivating flask
US20030077816A1 (en) 2000-12-27 2003-04-24 Richard Kronenthal Bioreactor and method for using
US20020155594A1 (en) 2001-03-27 2002-10-24 Hsieh Helen V. Method and apparatus for culturing cells
KR20020088848A (ko) 2001-05-21 2002-11-29 (주)코아바이오텍 세포배양관 및 이를 이용한 대량 세포배양기
US20040072347A1 (en) 2001-07-27 2004-04-15 Gerold Schuler Generation of fully mature and stable dendritic cells from leukaphereses products for clinical applications
AU2002367057B2 (en) 2001-12-21 2007-01-11 Organogenesis Inc. Chamber with adjustable volume for cell culture and organ assist
US7033823B2 (en) 2002-01-31 2006-04-25 Cesco Bioengineering, Inc. Cell-cultivating device
US7507579B2 (en) 2002-05-01 2009-03-24 Massachusetts Institute Of Technology Apparatus and methods for simultaneous operation of miniaturized reactors
US7229820B2 (en) 2002-06-13 2007-06-12 Wilson Wolf Manufacturing Corporation Apparatus and method for culturing and preserving tissue constructs
US20040029266A1 (en) 2002-08-09 2004-02-12 Emilio Barbera-Guillem Cell and tissue culture device
WO2004020065A2 (en) 2002-08-28 2004-03-11 Mt Technologies, Inc. Microfluidic affinity system using polydimethylsiloxane and a surface modification process
US20040067585A1 (en) 2002-10-07 2004-04-08 Yu-Chi Wang Cell cultivation surface and method of making the same
US20050032205A1 (en) 2003-08-05 2005-02-10 Smith Sidney T. In vitro cell culture employing a fibrin network in a flexible gas permeable container
JP2007511205A (ja) 2003-10-08 2007-05-10 ウィルソン ウォルフ マニュファクチャリング コーポレイション 気体透過性物質を利用する細胞を培養する方法及び装置
JP4649224B2 (ja) 2005-02-09 2011-03-09 株式会社カネカ 付着性細胞の培養方法および培養装置
US9014438B2 (en) 2005-08-17 2015-04-21 Koninklijke Philips N.V. Method and apparatus featuring simple click style interactions according to a clinical task workflow
JP5659448B2 (ja) 2006-08-23 2015-01-28 株式会社フコク 培養容器及び同容器を用いた細胞培養方法
US7897379B2 (en) 2007-02-26 2011-03-01 Corning Incorporated Device and method for reducing bubble formation in cell culture
US20090160975A1 (en) 2007-12-19 2009-06-25 Ncr Corporation Methods and Apparatus for Improved Image Processing to Provide Retroactive Image Focusing and Improved Depth of Field in Retail Imaging Systems
US8518692B2 (en) 2008-07-08 2013-08-27 Wilson Wolf Manufacturing Corporation Gas permeable cell culture device and method of use

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693537A (en) * 1994-06-28 1997-12-02 Wilson; John R. Compartmentalized tissue culture flask
US6562616B1 (en) * 1999-06-21 2003-05-13 The General Hospital Corporation Methods and devices for cell culturing and organ assist systems
US20050148068A1 (en) * 2003-12-31 2005-07-07 Lacey William J. Cell cultivating flask
US20070026516A1 (en) * 2005-07-26 2007-02-01 Martin Gregory R Multilayered cell culture apparatus
WO2008073314A2 (en) * 2006-12-07 2008-06-19 Wilson Wolf Manufacturing Corporation Highly efficient devices and methods for culturing cells

Also Published As

Publication number Publication date
AU2009268666B2 (en) 2014-11-13
US20100055774A1 (en) 2010-03-04
EP2297295A2 (en) 2011-03-23
CA2730104A1 (en) 2010-01-14
AU2009268666A1 (en) 2010-01-14
WO2010006055A3 (en) 2010-03-04
US8518692B2 (en) 2013-08-27
JP2011527578A (ja) 2011-11-04
JP5395171B2 (ja) 2014-01-22
EP2297295A4 (en) 2013-11-27
WO2010006055A2 (en) 2010-01-14
CN102089420A (zh) 2011-06-08

Similar Documents

Publication Publication Date Title
CN102089420B (zh) 改进的气体可渗透性细胞培养装置及使用方法
US12264332B2 (en) Highly efficient gas permeable devices and methods for culturing cells
AU2019240734B2 (en) Cell culture methods and devices utilizing gas permeable materials
EP2277986B1 (en) Multilayer cell culture vessel
AU2013200127A1 (en) Highly efficient gas permeable devices and methods for culturing cells

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant