CN102078485A - Preparation method and application of compound dogwood preparation - Google Patents

Abstract

The invention belongs to the technical field of medicine product research and development, and relates to a preparation method and application of a compound dogwood preparation. The preparation method comprises the steps of: selecting 9-12g of dogwood, 6-9g of acanthopanax root, 6-9g of eucommia bark, 6-gg of ginseng, 3-6g of lucid ganoderma, 3-6g of astragalus membranaceus, 3-6g of rhodiola rosea, 3-6g of mushroom, 2-4g of poria cocos, 2-4g of umbellate pore fungus, 2-4g of yam, 2-4g of hawthorn and 2-4g of Chinese date, with high quality, and cleaning, drying with microwave, crushing into 50-mu powder, adding water for boiling and extracting 3 times, mixing extract solutions, filtering to obtain filtrate, concentrating the filtrate into an extract, uniformly mixing in the proportion of the extract to agar to starch of 4:1:16, pelleting with a 10-mu sieve, and drying and then screening with a 14-mu sieve to obtain the compound dogwood preparation. The product has certain functions of adjusting and promoting the kidney function of the fancy carps, and is beneficial to the industrialized production.

Description

A kind of preparation method and its usage of compound recipe Fructus Corni preparation

Technical field

Medical product research and development technical field under the present invention.The present invention relates to a kind of preparation method and its usage of compound recipe Fructus Corni preparation.Product of the present invention has certain adjusting facilitation to the renal function of Cyprinus carpio L., and product of the present invention is beneficial to suitability for industrialized production.

Background technology

Advantage such as that Chinese herbal feed additive has is natural, economic, efficient, noresidue, no Drug resistance and side effect are little, and have the dual function of medical and trophism concurrently, the drawback of no antibiotics and chemicals, be widely used in recent years in the aquaculture of aquatic animal, in order to prevent disease, growth promotion with improve aquatic product quality.Contain the various active composition in the Chinese herbal medicine, the scalable immunity function of animal.Therefore mainly can to improve the animal body immunologic function relevant with it to it is generally acknowledged Chinese herbal medicine prevent disease and growth promoting function.The feed additive of Fish has shortcomings such as easy thawing, poor stability at present, and a part can not be eaten by fish, and utilization rate is low, and waste is serious.Agar (Agar) claims agar again, is the hydrophilic macromolecule polysaccharide that extracts from the Bostrychia montagnei guiding principle, has good gelling and stability, be widely used in aspects such as food, medicine, biological engineering, but the application in the fish feed additive is not reported.Blood urea nitrogen (BUN) is the main dead end product of protein metabolism, and under the normal situation of renal function, the variation of serum urea nitrogen remains in certain scope.When blood serum urea nitrogen concentration raises, decreased renal function is described.In this test, compare with matched group, the Cyprinus carpio L. serum urea nitrogen content of the Chinese medicine formula of throwing something and feeding is downward trend.Illustrate that Chinese medicine formula does not have Toxicity of Kidney and the renal function of Cyprinus carpio L. is had certain adjusting facilitation.

Summary of the invention

1, a kind of preparation method of compound recipe Fructus Corni preparation is characterized in that the preparation method of said preparation comprises following process:

Step (one): select high-quality Fructus Corni 9g-12g, Radix Et Caulis Acanthopanacis Senticosi 6g-9g, Cortex Eucommiae 6g-9g, Radix Ginseng 6g-9g, Ganoderma 3g-6g, Radix Astragali 3g-6g, Radix Rhodiolae 3g-6g, Lentinus Edodes 3g-6g, Poria 2g-4g, Polyporus 2g-4g, Rhizoma Dioscoreae 2g-4g, Fructus Crataegi 2g-4g, Fructus Jujubae 2g-4g and clean, microwave drying, pulverizing are 50 order powder;

Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0h-6.0h, heated and boiled is extracted 2.0h-3.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h-2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h-1.5h, merge extractive liquid, filters, and gets filtrate;

Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Fructus Corni preparation.

2, the purposes of a kind of compound recipe Fructus Corni preparation as claimed in claim 1 is characterised in that its renal function to Cyprinus carpio L. has certain adjusting facilitation.

According to the present invention, " % " among the present invention is percentage by weight.

The specific embodiment

The preparation method and its usage that relates to a kind of compound recipe Fructus Corni preparation of the present invention comprises following examples, and the following examples can further specify the present invention, but do not limit the present invention in any way.

Embodiment 1:

Step (one): select high-quality Fructus Corni 9g, Radix Et Caulis Acanthopanacis Senticosi 6g, Cortex Eucommiae 6g, Radix Ginseng 6g, Ganoderma 3g, Radix Astragali 3g, Radix Rhodiolae 3g, Lentinus Edodes 3g, Poria 2g, Polyporus 2g, Rhizoma Dioscoreae 2g, Fructus Crataegi 2g, Fructus Jujubae 2g and clean, microwave drying, pulverizing is 50 order powder;

Step (two): the powder in the step () is added water boil extract 3 times, add 6 times of water gagings first and soak 4.0h, heated and boiled is extracted 2.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h, merge extractive liquid,, filter, get filtrate;

Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Fructus Corni preparation.

Embodiment 2:

Step (one): select high-quality Fructus Corni 12g, Radix Et Caulis Acanthopanacis Senticosi 9g, Cortex Eucommiae 9g, Radix Ginseng 9g, Ganoderma 6g, Radix Astragali 6g, Radix Rhodiolae 6g, Lentinus Edodes 6g, Poria 4g, Polyporus 4g, Rhizoma Dioscoreae 4g, Fructus Crataegi 4g, Fructus Jujubae 4g and clean, microwave drying, pulverizing are 50 order powder;

Step (two): the powder in the step () is added water boil extract 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h, add for the second time 4 times of water gaging heated and boiled and extract 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid,, filter, get filtrate;

Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Fructus Corni preparation.

Pharmacological evaluation

Compound recipe Fructus Corni preparation is to the adjusting facilitation experiment of Cyprinus carpio L. renal function

1 materials and methods

1.1 material

1.1.1 compound recipe Fructus Corni preparation

Select high-quality Fructus Corni 12g, Radix Et Caulis Acanthopanacis Senticosi 9g, Cortex Eucommiae 9g, Radix Ginseng 9g, Ganoderma 6g, Radix Astragali 6g, Radix Rhodiolae 6g, Lentinus Edodes 6g, Poria 4g, Polyporus 4g, Rhizoma Dioscoreae 4g, Fructus Crataegi 4g, Fructus Jujubae 4g also cleans, microwave drying, pulverizing is 50 order powder, powder is added water boil to be extracted 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h, add for the second time 4 times of water gaging heated and boiled and extract 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid,, filter, filtrate, filtrate is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Fructus Corni preparation.

1.1.2 experiment feedstuff

By compound recipe Fructus Corni preparation: the mixed uniform preparation of normal feedstuff=3: 5 is a compound recipe Fructus Corni preparation experiment feedstuff, does blank with normal feedstuff.Experiment normal feedstuff prescription sees Table 1.

Table 1 experiment is filled a prescription with normal feedstuff

1.2 method

1.2.1 experiment is raised with Cyprinus carpio L.

Experiment uses Cyprinus carpio L. Cyprinus carpio L. available from Aquatic product market, Weihai, body weight (41.1 ± 5.0) g, and long (10.27 ± 0.48) cm of body, the male and female dual-purpose is supported in indoor glass aquarium before the experiment beginning temporarily, with commercial feed it is satiated with food and throws something and feeds.As source of recycled water, 24h continues aeration aerating, keeps dissolved oxygen in water amount 5.0mg/L with the tap water of abundant aeration dechlorination, and water temperature is controlled at (21 ± 1) ℃, raises and train to begin after 7 days to test.Experiment is divided into 2 groups with Cyprinus carpio L. at random by body weight, and experimental group is with compound recipe Fructus Corni preparation experiment feedstuff feeding, the matched group normal feedstuff of throwing something and feeding, every group 20 tail, establish 3 parallel.Experiment periods every other day feeding volume is 2%～3% of a fish body constitution amount, and regularly throw something and feed during 7:00 and during 19:00 every day, and be 15 days experimental period.

1.2.2 measure the collection of sample

Off-test began jejunitas 24h in preceding 1 day, got 5 tail fishes at random from each aquarium, got blood with disposable syringe tail vein, left standstill 2h under 4 ℃, and the centrifugal 15min of 3000r/min draws supernatant and prepares serum then, is put in-4 ℃ of refrigerators and preserves standby.

1.2.3 the mensuration of serum urea nitrogen

Take out the serum of freezing preservation, adopt the Fearon reaction assay serum urea nitrogen (BUN) of BUN test kit (bio-engineering research institute, lot number 20101101 are built up in Nanjing).

2 results

Compound recipe Fructus Corni preparation sees Table 2 to the influence of Cyprinus carpio L. serum urea nitrogen.

Table 2 compound recipe Fructus Corni preparation is to the influence of Cyprinus carpio L. serum urea nitrogen

Annotate: compare * P＜005 with matched group.

By table 2 as seen, compound recipe Fructus Corni preparation can significantly reduce the content of Cyprinus carpio L. serum urea nitrogen.

Claims (2)

1. the preparation method of a compound recipe Fructus Corni preparation is characterized in that the preparation method of said preparation comprises following process:

Step (one): select high-quality Fructus Corni 9g-12g, Radix Et Caulis Acanthopanacis Senticosi 6g-9g, Cortex Eucommiae 6g-9g, Radix Ginseng 6g-9g, Ganoderma 3g-6g, Radix Astragali 3g-6g, Radix Rhodiolae 3g-6g, Lentinus Edodes 3g-6g, Poria 2g-4g, Polyporus 2g-4g, Rhizoma Dioscoreae 2g-4g, Fructus Crataegi 2g-4g, Fructus Jujubae 2g-4g and clean, microwave drying, pulverizing are 50 order powder;

Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0h-6.0h, heated and boiled is extracted 2.0h-3.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h-2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h-1.5h, merge extractive liquid, filters, and gets filtrate;

Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: agar: starch=4: 1: 16 ratio mixings, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Fructus Corni preparation.

2. the purposes of a kind of compound recipe Fructus Corni preparation as claimed in claim 1 is characterised in that its renal function to Cyprinus carpio L. has certain adjusting facilitation.