CN102050843A - 一种从茶叶中提取生物碱、儿茶素的方法 - Google Patents
一种从茶叶中提取生物碱、儿茶素的方法 Download PDFInfo
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- CN102050843A CN102050843A CN201010552546XA CN201010552546A CN102050843A CN 102050843 A CN102050843 A CN 102050843A CN 201010552546X A CN201010552546X A CN 201010552546XA CN 201010552546 A CN201010552546 A CN 201010552546A CN 102050843 A CN102050843 A CN 102050843A
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- China
- Prior art keywords
- tealeaves
- catechin
- ethanolic soln
- alkaloid
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 title claims abstract description 38
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 235000005487 catechin Nutrition 0.000 title claims abstract description 38
- 229950001002 cianidanol Drugs 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 34
- 229930013930 alkaloid Natural products 0.000 title claims abstract description 28
- 150000003797 alkaloid derivatives Chemical class 0.000 title claims abstract description 28
- 241001122767 Theaceae Species 0.000 title abstract 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 39
- 238000005227 gel permeation chromatography Methods 0.000 claims abstract description 21
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- 239000012507 Sephadex™ Substances 0.000 claims abstract description 8
- 238000002203 pretreatment Methods 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 7
- 239000012141 concentrate Substances 0.000 claims description 17
- 239000000706 filtrate Substances 0.000 claims description 14
- 239000000284 extract Substances 0.000 claims description 11
- 238000004587 chromatography analysis Methods 0.000 claims description 9
- XELZGAJCZANUQH-UHFFFAOYSA-N methyl 1-acetylthieno[3,2-c]pyrazole-5-carboxylate Chemical group CC(=O)N1N=CC2=C1C=C(C(=O)OC)S2 XELZGAJCZANUQH-UHFFFAOYSA-N 0.000 claims description 9
- 229920002684 Sepharose Polymers 0.000 claims description 6
- 238000005374 membrane filtration Methods 0.000 claims description 5
- 238000003809 water extraction Methods 0.000 claims description 4
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- 238000000926 separation method Methods 0.000 abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 8
- 238000001914 filtration Methods 0.000 abstract description 5
- 239000003960 organic solvent Substances 0.000 abstract description 5
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- RYYVLZVUVIJVGH-UHFFFAOYSA-N trimethylxanthine Natural products CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 15
- YAPQBXQYLJRXSA-UHFFFAOYSA-N theobromine Chemical compound CN1C(=O)NC(=O)C2=C1N=CN2C YAPQBXQYLJRXSA-UHFFFAOYSA-N 0.000 description 11
- LCCDINSFSOALJK-UHFFFAOYSA-N 1,3,4-trimethylpurine-2,6-dione Chemical compound O=C1N(C)C(=O)N(C)C2(C)N=CN=C21 LCCDINSFSOALJK-UHFFFAOYSA-N 0.000 description 6
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- 238000004440 column chromatography Methods 0.000 description 6
- 238000004108 freeze drying Methods 0.000 description 5
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- 238000011084 recovery Methods 0.000 description 5
- 229960004559 theobromine Drugs 0.000 description 5
- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 description 4
- 229960001948 caffeine Drugs 0.000 description 4
- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 description 4
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- WMBWREPUVVBILR-WIYYLYMNSA-N (-)-Epigallocatechin-3-o-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-WIYYLYMNSA-N 0.000 description 3
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- WMBWREPUVVBILR-UHFFFAOYSA-N GCG Natural products C=1C(O)=C(O)C(O)=CC=1C1OC2=CC(O)=CC(O)=C2CC1OC(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-UHFFFAOYSA-N 0.000 description 3
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- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
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- 235000013361 beverage Nutrition 0.000 description 2
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- 239000012467 final product Substances 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
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- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- CPJSUEIXXCENMM-UHFFFAOYSA-N phenacetin Chemical compound CCOC1=CC=C(NC(C)=O)C=C1 CPJSUEIXXCENMM-UHFFFAOYSA-N 0.000 description 2
- PFTAWBLQPZVEMU-UKRRQHHQSA-N (-)-epicatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-UKRRQHHQSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 description 1
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- 208000001953 Hypotension Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229960001138 acetylsalicylic acid Drugs 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
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- 238000004458 analytical method Methods 0.000 description 1
- 239000000730 antalgic agent Substances 0.000 description 1
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- 235000006708 antioxidants Nutrition 0.000 description 1
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- 230000001882 diuretic effect Effects 0.000 description 1
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- 235000019253 formic acid Nutrition 0.000 description 1
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- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
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- 239000000463 material Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229960003893 phenacetin Drugs 0.000 description 1
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- 230000004223 radioprotective effect Effects 0.000 description 1
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
Claims (10)
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CN 201010552546 CN102050843B (zh) | 2010-11-22 | 2010-11-22 | 一种从茶叶中提取生物碱、儿茶素的方法 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108014517A (zh) * | 2017-12-20 | 2018-05-11 | 泰州医药城国科化物生物医药科技有限公司 | 一种利用多糖基介质对原小檗碱型生物碱富集纯化的方法 |
CN113150039A (zh) * | 2021-05-13 | 2021-07-23 | 襄阳嘉达生物科技有限公司 | 一种从绿茶中提取生物碱的方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1557841A (zh) * | 2004-01-14 | 2004-12-29 | 中国农业科学院茶叶研究所 | 从茶叶中综合提取茶多糖、茶多酚、茶氨酸、咖啡碱的方法 |
CN1562995A (zh) * | 2004-04-02 | 2005-01-12 | 胡绍海 | 高纯茶多酚和单体儿茶素的制备工艺及其装置 |
CN1872172A (zh) * | 2005-05-31 | 2006-12-06 | 卡夫食品研发公司 | 从可可壳提取的富多酚组合物 |
CN101386614A (zh) * | 2008-10-24 | 2009-03-18 | 集美大学 | 树脂吸附法制备表没食子儿茶素没食子酸酯的方法 |
CN101508690A (zh) * | 2009-03-17 | 2009-08-19 | 林峰 | 一种从茶叶中同时提取茶多酚、茶多糖、咖啡因的新方法 |
CN101665460A (zh) * | 2009-08-07 | 2010-03-10 | 南京中医药大学 | 一种新结构类型化合物莲子心新碱及其分离鉴定方法 |
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2010
- 2010-11-22 CN CN 201010552546 patent/CN102050843B/zh not_active Expired - Fee Related
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1557841A (zh) * | 2004-01-14 | 2004-12-29 | 中国农业科学院茶叶研究所 | 从茶叶中综合提取茶多糖、茶多酚、茶氨酸、咖啡碱的方法 |
CN1562995A (zh) * | 2004-04-02 | 2005-01-12 | 胡绍海 | 高纯茶多酚和单体儿茶素的制备工艺及其装置 |
CN1872172A (zh) * | 2005-05-31 | 2006-12-06 | 卡夫食品研发公司 | 从可可壳提取的富多酚组合物 |
CN101386614A (zh) * | 2008-10-24 | 2009-03-18 | 集美大学 | 树脂吸附法制备表没食子儿茶素没食子酸酯的方法 |
CN101508690A (zh) * | 2009-03-17 | 2009-08-19 | 林峰 | 一种从茶叶中同时提取茶多酚、茶多糖、咖啡因的新方法 |
CN101665460A (zh) * | 2009-08-07 | 2010-03-10 | 南京中医药大学 | 一种新结构类型化合物莲子心新碱及其分离鉴定方法 |
Non-Patent Citations (4)
Title |
---|
HIROSHI HORITA.: "Extraction and purification of catechins from tea", 《PROCEEDING OF INTERNATIONAL SYMPOSIUM ON TEA SCIENCE》, 29 August 1991 (1991-08-29), pages 625 - 629 * |
李平等: "茶叶茶多酚、咖啡碱提取及超滤-吸附综合分离纯化过程研究", 《科研与开发》, vol. 19, no. 11, 20 November 1999 (1999-11-20), pages 21 - 24 * |
车红荣: "茶叶茶多酚的乙醇水溶液提取过程、离子沉淀-絮凝纯化新工艺研究", 《西北大学硕士学位论文》, 1 June 2001 (2001-06-01), pages 1 - 37 * |
高永贵: "茶叶抗氧化剂的制备方法综述", 《茶叶》, vol. 22, no. 2, 10 June 1996 (1996-06-10) * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108014517A (zh) * | 2017-12-20 | 2018-05-11 | 泰州医药城国科化物生物医药科技有限公司 | 一种利用多糖基介质对原小檗碱型生物碱富集纯化的方法 |
CN113150039A (zh) * | 2021-05-13 | 2021-07-23 | 襄阳嘉达生物科技有限公司 | 一种从绿茶中提取生物碱的方法 |
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