CN102010802A - Concentrated multienzyme cleaning solution and preparation method thereof - Google Patents
Concentrated multienzyme cleaning solution and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a concentrated multienzyme cleaning solution and a preparation method thereof, wherein the concentrated multienzyme cleaning solution is prepared from the following raw materials in parts by weight: 1 to 5 parts of organic preservative, 200 to 400 parts of surfactant, 20 to 65 parts of enzyme stabilizer, 40 to 80 parts of propylene glycol, 30 to 80 parts of triethanolamine, 10 to 60 parts of sodium sulfite, 0.1 to 5 parts of calcium chloride, 20 to 60 parts of sodium citrate, 10 to 65 parts of citric acid, 0.1 to 1 part of antifoaming agent, 30 to 80 parts of bio-enzyme, and appropriate deionized water; and the method for preparing the concentrated multienzyme cleaning solution comprises the following steps: dissolving the preservative into the propylene glycol, then adding the surfactant, the triethanolamine and the antifoaming agent into the obtained mixture so as to obtain a solution A; heating the deionized water, and dissolving the enzyme stabilizer, the sodium sulfite, the calcium chloride, the sodium citrate and the citric acid into the deionized water, then cooling the obtained mixture so as to obtain a solution bio-enzyme B; and finally, mixing the solution A and the solution B so as to obtain the finished product of the concentrated multienzyme cleaning solution. The concentrated multienzyme cleaning solution and the preparation method thereof have the advantages: the enzyme stabilizer is adopted to effectively protect the activity of the bio-enzyme, and used with the surfactant and the preservative, so that the obtained finished product is stable in performance, long in storage time and strong in capacity of decomposing biological tissues.
Description
Technical field
The present invention relates to a kind of washings, especially relate to a kind of medical concentrated type multienzyme washings, the invention still further relates to the preparation method of this washings.
Background technology
Some instruments that hospital uses, checking tool etc. need to reuse, these utensils can be infected with patient's body fluid and tissue behind the contact patient, if clean not thoroughly, not only influence sterilisation effect, cause iatrogenic infection and is affected the work-ing life of apparatus.The cutter that the slaughterhouse is used, loading transportation means, staff's working suit etc. all is reusable, if the cleaning to animal tissues, blood is not enough, not only influence the environmental health in slaughterhouse, and can provide the chance of propagating for take a disease the altogether cause of disease of disease of people and animals.Above-mentioned these special articles all rely on multienzyme detergent to clean at present.
In the washing composition production, the application of the enzyme of biologically active in Betengent product is commonplace.Amylase, cellulase, the lipase used finally by single proteolytic enzyme of initial stage, or with the synergistic application of two or more enzyme be that the over-all properties of washing composition improves, quality product improves and made huge contribution.Because enzyme itself is protein, have nutritive value, very easily utilized or be subjected to the influence of extraneous physics, chemical factor and sex change, thereby lose activity by microorganism.Simultaneously, various enzymes are in active condition in liquid washing agent, proteolytic enzyme can decompose other enzyme of destruction, even decomposition of protein enzyme self, therefore other enzyme and proteolytic enzyme are in same system, biologically active labile also can utilize reasons such as degraded or proteolytic enzyme self degraded to make the storage life shorter because of the protein volatility or by microorganism even contain the liquid washing agent of single proteolytic enzyme, influences washing effect.And,, be beneficial to storage though do not disturb mutually between each enzyme with the multienzyme detergent that solid exists, and to earlier the solid particulate dissolving that contains multienzyme be scattered in the water when using, discharge enzyme, produce activity, use comparatively inconvenience like this; In addition, in dissolution process, the water-soluble bad of some enzymes arranged, can influence the performance of enzymic activity, also can influence washing effect.
Summary of the invention
The object of the present invention is to provide a kind of stable performance, the shelf lives is long, washing effect is good concentrated type multienzyme washings, the present invention also provides the preparation method of this washings.
For achieving the above object, the present invention can take following technical proposals:
Concentrated type multienzyme washings of the present invention, it is by 1～5 part of organic anti-corrosive agent, 200～400 parts in tensio-active agent, 20～65 parts of enzyme stabilizers, 40～80 parts of propylene glycol, 30～80 parts of trolamines, 10～60 parts of S-WATs, 0.1～5 part in calcium chloride, 20～60 parts of Trisodium Citrates, 10～65 parts of citric acids, 0.1～1 part of defoamer, 30～80 parts of biological enzymes, deionized water is formulated in right amount; Described organic anti-corrosive agent be Tegosept M or/and ethyl p-hydroxybenzoate or/and propylben; Described tensio-active agent be polyoxyethylenated alcohol sodium sulfate or/and the cocamidopropyl propyl amide amine oxide or/and cocoanut fatty acid diethanolamide or/and the dodecanamide propyl amine oxide or/and alkyl glycoside; Described enzyme stabilizers is that Sodium Benzoate is or/and sodium acetate; Described defoamer is that organosilicon is or/and sucrose ester; Described biological enzyme be proteolytic enzyme or/and lipase or/and cellulase or/and amylase.
The preparation method of concentrated type multienzyme washings of the present invention is:
The first step is dissolved in the organic anti-corrosive agent in the propylene glycol, adds tensio-active agent, trolamine and defoamer again, and it is standby to make solution A;
In second step, the deionized water heating after enzyme stabilizers, S-WAT, calcium chloride, Trisodium Citrate and citric acid dissolving, being cooled to room temperature, adds biological enzyme again, and it is standby to make solution B;
In the 3rd step, will can obtain concentrated type multienzyme washings finished product after solution A and the solution B mixing.
After getting an amount of this concentrated cleaning solution thin up during use, the machine washing hand washing all can, easy to use, be easy to rinsing.
The invention has the advantages that and utilize enzyme stabilizers Sodium Benzoate, sodium acetate effectively to protect biological enzyme activity; prevent that the mutual decomposition between the biological enzyme from destroying; the synergy that cooperates kinds of surface promoting agent, sanitas again; make Betengent product stable performance of the present invention, shelf lives long, the ability of decomposing organism tissue is strong.The multiple high-activity bio-enzyme that contains in the concentrated cleaning solution of the present invention, act synergistically during washing, can rapidly, effectively decompose human secretion and biological tissue, both reduced the high maintenance service of instruments, can reach better sterilisation effect again, avoid because of cleaning big workload of thoroughly not bringing and the phenomenon that subinfection occurs again.This washings is suitable for cleaning cutter in various medicine equipments, hospital surgery sheet, the slaughterhouse and loading appliance, working suit etc., for the operating scissors of easily leaving over material in the endoscope that is easy to stop up, the crack, mosquito forceps etc. better cleaning effect is arranged also.
2 kinds of multienzyme washing lotions (A and B) that the sales volume that washings products and marketing of the present invention is buied is bigger adopt kapillary identification method and standard soiled cloth method to carry out the washing effect contrast, and experimental result is as follows:
1, kapillary identification method
(1), get product 1mL of the present invention and commercially available prod A and each 1mL of B and be positioned over respectively in the 500mL beaker, in beaker, add ordinary tap water respectively and be diluted to 400 times; In addition, in the beaker of 1 500mL, add the 400mL ordinary tap water as blank;
(2), getting 200 kapillaries altogether, to draw the fresh rabbit blood of equivalent standby;
(3), the kapillary of the fresh rabbit blood of absorption in the step (2) is divided into identical 4 parts of quantity, promptly 50 every part, put into the beaker that step (1) is prepared respectively;
(4), the kapillary in the described beaker of step (3) was washed 10 minutes with ultrasonic cleaner respectively;
(5), respectively handle triplicate, estimate washing effect with mean number.
The wash result of this experiment is: the present invention cleans 50 of pipe number average out to, and the clean pipe of commercially available prod A is counted 19 of average out to, and the clean pipe of commercially available prod B is counted 13 of average out to, and several 0 of the clean pipe of blank.
2, standard soiled cloth method
(1), get product 1mL of the present invention and commercially available prod A and each 1mL of B and be positioned over respectively in the 500mL beaker, in beaker, add ordinary tap water respectively and be diluted to 400 times; In addition, in the beaker of 1 500mL, add the 400mL ordinary tap water as blank;
(2), the cotton calico is cut into 4 of the rectangle cloths of the wide 5cm of long 10cm, rectangle cotton calico is immersed in the fresh rabbit blood, takes out after 5 minutes and dry;
(3), be soaked with rabbit blood in the step (2) and dry after rectangle cotton calico be cut into two with every respectively, the square of the wide 5cm of promptly long 5cm, and in the beaker that step (1) is prepared, put into two cloths respectively;
(4), the described beaker of step (3) was washed in ultrasonic cleaner 10 minutes;
(5), the cotton calico of the wide 5cm of two block length 5cm in each beaker is dried respectively, then, washing effect relatively;
(6), respectively handle triplicate, with mean number as estimating foundation.
The washing effect evaluation method: thoroughly to clean be 3 minutes to bloodstain on the cotton, and to clean fully be 2 minutes to bloodstain on the cotton, on the cotton bloodstain with clean before not too big difference be 1 minute.
The washing effect of this experiment is: the present invention must be divided into 3 fens, and commercially available prod B must be divided into 2 fens, and commercially available prod A and blank are 1 minute.
In addition, the various surgical instruments of once using in the laboratory with washings cleaning experiment zootomy of the present invention, washing effect is fine.
Embodiment
Embodiment 1:
Concentrated type multienzyme washings of the present invention is by raw material Tegosept M 1 gram, ethyl p-hydroxybenzoate 0.5 gram, polyoxyethylenated alcohol sodium sulfate 55 grams, cocoanut fatty acid diethanolamide 200 grams, Sodium Benzoate 50 grams, propylene glycol 55 grams, trolamine 60 grams, S-WAT 44.5 grams, calcium chloride 5 grams, Trisodium Citrate 60 grams, citric acid 65 grams, organosilicon 0.05 gram, sucrose ester (SE-3) 0.5 gram, proteolytic enzyme 12 grams, lipase 9 grams, amylase 5 grams, cellulase 4 grams, deionized water 373.45 grams are formulated.Concrete preparation method is:
Tegosept M and ethyl p-hydroxybenzoate are dissolved in the propylene glycol, add trolamine then, polyoxyethylenated alcohol sodium sulfate, cocoanut fatty acid diethanolamide, organosilicon, sucrose ester (SE-3), it is standby to make solution A;
Deionized water is heated to 60 ℃～70 ℃, adds Sodium Benzoate, S-WAT, calcium chloride, Trisodium Citrate, citric acid is cooled to room temperature, adds proteolytic enzyme more successively, lipase, amylase and cellulase are made solution B;
To can obtain concentrated type multienzyme washings finished product after solution A and the solution B mixing.
Embodiment 2:
Concentrated type multienzyme washings of the present invention is by raw material ethyl p-hydroxybenzoate 1 gram, polyoxyethylenated alcohol sodium sulfate 60 grams, dodecanamide propyl amine oxide 180 grams, cocamidopropyl propyl amide amine oxide 50 grams, sodium acetate 15 grams, Sodium Benzoate 30 grams, propylene glycol 40 grams, trolamine 80 grams, S-WAT 20 grams, calcium chloride 1 gram, Trisodium Citrate 35 grams, citric acid 45 grams, organosilicon 1 gram, protease 30 gram, lipase 18 grams, amylase 4 grams, cellulase 12 grams, deionized water 378 grams are formulated.Concrete preparation method is:
Ethyl p-hydroxybenzoate is dissolved in the propylene glycol, adds trolamine then, polyoxyethylenated alcohol sodium sulfate, the dodecanamide propyl amine oxide, the cocamidopropyl propyl amide amine oxide, organosilicon, it is standby to make solution A;
Deionized water is heated to 60 ℃～70 ℃, the dissolving sodium acetate, Sodium Benzoate, S-WAT, calcium chloride, Trisodium Citrate, citric acid is cooled to room temperature, adds proteolytic enzyme more successively, lipase, amylase, cellulase is made solution B;
To can obtain concentrated type multienzyme washings finished product after solution A and the solution B mixing.
Embodiment 3:
Concentrated type multienzyme washings of the present invention is by raw material Tegosept M 1.5 grams, propylben 3 grams, polyoxyethylenated alcohol sodium sulfate 45 grams, alkyl glycoside 150 grams, cocamidopropyl propyl amide amine oxide 105 grams, sodium acetate 55 grams, propylene glycol 50 grams, trolamine 55 grams, S-WAT 35 grams, calcium chloride 0.8 gram, Trisodium Citrate 50 grams, citric acid 18 grams, sucrose ester (SE-3) 0.8 gram, proteolytic enzyme 20 grams, lipase 23 grams, amylase 14 grams, cellulase 10 grams, deionized water 363.9 grams are formulated.Concrete preparation method is:
Tegosept M, propylben are dissolved in the propylene glycol, add trolamine successively, polyoxyethylenated alcohol sodium sulfate, alkyl glycoside, the cocamidopropyl propyl amide amine oxide, sucrose ester (SE-3), it is standby to make solution A;
Deionized water is heated to 60 ℃～70 ℃, dissolves sodium acetate successively, S-WAT, calcium chloride, Trisodium Citrate, citric acid is cooled to room temperature, adds proteolytic enzyme more successively, lipase, amylase, cellulase is made solution B;
To can obtain concentrated type multienzyme washings finished product after solution A and the solution B mixing.
Embodiment 4:
Concentrated type multienzyme washings of the present invention is by raw material ethyl p-hydroxybenzoate 0.5 gram, propylben 1.5 grams, alkyl glycoside 100 grams, cocoanut fatty acid diethanolamide 150 grams, dodecanamide propyl amine oxide 80 grams, sodium acetate 38 grams, propylene glycol 72 grams, trolamine 70 grams, S-WAT 25 grams, calcium chloride 2.5 grams, Trisodium Citrate 20 grams, citric acid 30 grams, sucrose ester (SE-1) 0.1 gram, proteolytic enzyme 24 grams, lipase 10 grams, amylase 5 grams, cellulase 2 grams, deionized water 369.4 grams are formulated.Concrete preparation method is:
Ethyl p-hydroxybenzoate and propylben are dissolved in the propylene glycol, add trolamine successively, alkyl glycoside, cocoanut fatty acid diethanolamide, the dodecanamide propyl amine oxide, sucrose ester (SE-1), it is standby to make solution A;
Deionized water is heated to 60 ℃～70 ℃, dissolves sodium acetate successively, S-WAT, calcium chloride, Trisodium Citrate, citric acid is cooled to room temperature, adds proteolytic enzyme successively, lipase, amylase, cellulase is made solution B;
To can obtain concentrated type multienzyme washings finished product after solution A and the solution B mixing.
Embodiment 5:
Concentrated type multienzyme washings of the present invention is by raw material Tegosept M 0.5 gram, ethyl p-hydroxybenzoate 0.5 gram, propylben 1.5 grams, alkyl glycoside 120 grams, dodecanamide propyl amine oxide 80 grams, sodium acetate 65 grams, propylene glycol 65 grams, trolamine 65 grams, S-WAT 60 grams, calcium chloride 3.5 grams, Trisodium Citrate 40 grams, citric acid 58 grams, organosilicon 0.4 gram, sucrose ester (SE-3) 0.2 gram, protease 30 gram, lipase 26 grams, amylase 16 grams, cellulase 8 grams, deionized water 360.4 grams are formulated.Concrete preparation method is:
With Tegosept M, ethyl p-hydroxybenzoate and propylben are dissolved in the propylene glycol, add trolamine then successively, alkyl glycoside, and the dodecanamide propyl amine oxide, organosilicon, sucrose ester (SE-3), it is standby to make solution A;
Deionized water is heated to 60 ℃～70 ℃, dissolves S-WAT successively, calcium chloride, sodium acetate, Trisodium Citrate, citric acid; Be cooled to room temperature, add proteolytic enzyme successively, lipase, amylase, cellulase is made solution B;
To can obtain concentrated type multienzyme washings finished product after solution A and the solution B mixing.
Embodiment 6:
Concentrated type multienzyme washings of the present invention is by raw material Tegosept M 5 grams, dodecanamide propyl amine oxide 220 grams, alkyl glycoside 180 grams, Sodium Benzoate 20 grams, propylene glycol 80 grams, trolamine 30 grams, S-WAT 10 grams, calcium chloride 0.1 gram, Trisodium Citrate 20 grams, citric acid 10 grams, organosilicon 0.1 gram; Sucrose ester (SE-3) 0.3 gram, proteolytic enzyme 26 grams, lipase 15 grams, amylase 10 grams, cellulase 8 grams, deionized water 365.5 grams are formulated.Concrete preparation method is:
Tegosept M is dissolved in the propylene glycol, adds trolamine then successively, the dodecanamide propyl amine oxide, alkyl glycoside, organosilicon, sucrose ester (SE-3), it is standby to make solution A;
Deionized water is heated to 60 ℃～70 ℃, dissolves S-WAT successively, Sodium Benzoate, calcium chloride, Trisodium Citrate, citric acid is cooled to room temperature, adds proteolytic enzyme successively, lipase, amylase, cellulase is made solution B;
To can obtain concentrated type multienzyme washings finished product after solution A and the solution B mixing.
Claims (2)
1. concentrated type multienzyme washings, it is characterized in that: it is by 1～5 part of organic anti-corrosive agent, 200～400 parts in tensio-active agent, 20～65 parts of enzyme stabilizers, 40～80 parts of propylene glycol, 30～80 parts of trolamines, 10～60 parts of S-WATs, 0.1～5 part in calcium chloride, 20～60 parts of Trisodium Citrates, 10～65 parts of citric acids, 0.1～1 part of defoamer, 30～80 parts of biological enzymes, deionized water is formulated in right amount; Described organic anti-corrosive agent be Tegosept M or/and ethyl p-hydroxybenzoate or/and propylben; Described tensio-active agent be polyoxyethylenated alcohol sodium sulfate or/and the cocamidopropyl propyl amide amine oxide or/and cocoanut fatty acid diethanolamide or/and the dodecanamide propyl amine oxide or/and alkyl glycoside; Described enzyme stabilizers is that Sodium Benzoate is or/and sodium acetate; Described defoamer is that organosilicon is or/and sucrose ester; Described biological enzyme be proteolytic enzyme or/and lipase or/and cellulase or/and amylase.
2. concentrated type multienzyme washings preparation method according to claim 1, it is characterized in that: it comprises the steps:
The first step is dissolved in the organic anti-corrosive agent in the propylene glycol, adds tensio-active agent, trolamine and defoamer again, and it is standby to make solution A;
In second step, the deionized water heating after enzyme stabilizers, S-WAT, calcium chloride, Trisodium Citrate and citric acid dissolving, being cooled to room temperature, adds biological enzyme again, and it is standby to make solution B;
In the 3rd step, will can obtain concentrated type multienzyme washings finished product after solution A and the solution B mixing.
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