KR101998148B1 - Handwash composition using a fermentation solution of pear and lactic acid bacterium - Google Patents

Abstract

The present invention relates to a hand wash composition using a fermented broth and a lactic acid fermentation broth. More particularly, the present invention relates to a hand wash composition using a fermentation broth of lactic acid bacteria, A fermented lactic acid fermentation broth fermented by fermentation and a composition for cleaning a raw material from a natural origin are comprised of an effective ingredient and a hand wash composition using a lactic acid fermentation broth.
The present invention relates to a fermentation broth comprising 5 to 10% by weight of a lactic acid fermentation broth obtained by inoculating a fermented lactic acid bacterium in a broth or culture broth containing a distillate juice separated from a flesh pulp and cultivating the fermented broth, and 90 to 95% by weight of a cleaning composition as an active ingredient .

Description

TECHNICAL FIELD [0001] The present invention relates to a hand wash composition using a fermented broth and a lactic acid fermentation liquid,

The present invention relates to a hand wash composition using a fermented broth and a lactic acid fermentation broth. More particularly, the present invention relates to a hand wash composition using a fermentation broth of lactic acid bacteria, A fermented lactic acid fermentation broth fermented by fermentation and a composition for cleaning a raw material from a natural origin are comprised of an effective ingredient and a hand wash composition using a lactic acid fermentation broth.

In recent years, as the number of days of high dust concentration in the air has increased due to the yellow dust from China, people are increasingly interested in the health and hygiene fields. Therefore, the consumption of hand wash for washing fine dust and dirt in the hands is gradually increasing have.

An average of 10,000 to 10 million germs are inhabited by humans, and they are most likely to come into contact with the outside of the body, so they are susceptible to germs, By simply washing your hands with soap or hand wash, you can prevent various diseases.

Generally, Hand Wash is composed of a synthetic type surfactant composed of an active ingredient and a cleaning type which is to wash the dirt by the surface activity of the foam and the washing of the water, and isopropanol, isopropyl alcohol and the like, so that the hands are not washed, rubbed or rubbed, It is classified as a disinfection type which is killed and antibacterial. At this time, the ingredients of the hand wash are generally harmful to the human body because synthetic surfactants, artificial pigments and animal-derived ingredients are added to stimulate the skin.

Synthetic surfactants are collectively referred to as Sodium Lauryl Sulfate, Sodium Laureth Sulfate, Ammonium Lauryl Sulfate, and preservatives. They are excellent in detergency and are excellent in shampoo, laundry detergent, kitchen detergent, Soaps, bodywashes, etc. However, if it remains on the skin, it easily penetrates into the human body and destroys the skin wall. As a result, the moisture and sebum in the skin wash away and dry the skin, thereby causing atopy, allergic dermatitis, acne, swelling and wrinkles . ≪ / RTI >

The sodium lauryl sulfate and sodium laureth sulfate are anionic surfactants. They are cheap, spread the solution more easily in water, and are excellent in foaming power. They are effective for dandruff and seborrheic dermatitis because of high washing power for detergent, toothpaste, shampoo, Or mucous membranes, causing bacterial infections. Special attention should be given to people with atopic, sensitive skin or allergies.

Therefore, it is essential to always rinse cleanly so that the synthetic surfactant does not remain in the hands. Handwashes containing synthetic surfactants, animal-derived raw materials, preservatives, artificial coloring agents, etc., should be used by patients with sensitive skin, There is no problem.

A study on the adverse effects of synthetic surfactants on the human body has been published, and the alternative is EM (Effective Microorganism) detergent.

EM refers to 80 kinds of microorganisms such as yeast, yeast, lactobacillus, photosynthetic bacteria, actinomycetes and the like, and produces an effective amount of physiologically active substances such as odor elimination, water purification, prevention of oxidation of metal and food, It has the specificity of decomposing mold bacteria, viruses and mites into harmless substances. Therefore, the use of EM instead of conventional synthetic chemicals is being used and researched in various fields such as detergent, shampoo, soap, lactose, fertilizer and environment .

In order to solve the problems of the prior art by using EM, Korean Patent Laid-Open No. 10-2007-0003956 discloses a useful microorganism group containing lactic acid bacteria group, yeast group, photosynthetic bacterial group and antioxidant substances produced by the useful microorganism group A method for producing a detergent is proposed, which comprises mixing a concentrate into clay (aged), aging and then firing the ceramics powder as a raw material.

The cleaning agent applying the above fermentation technique and the manufacturing method thereof are used for fermenting and aging a raw material by a fermenting microorganism group containing a lactic acid bacteria group, a yeast group and a photosynthetic bacterial group of a tuberous anaerobic lactic acid bacteria, It is an invention that emphasizes the effect of purifying the sewage water by the proliferation of the beneficial microorganisms in the washed polluted water without maintaining the washing effect of the fine dust, but maintaining the soaking water in the soap.

Therefore, there is a need for a cleaning composition capable of harmlessly cleaning micro-dusts adhering to the body with a naturally derived raw material of vegetable origin excluding chemical additives, and capable of completely antibacterizing bacteria in fine dust.

 DISCLOSURE OF THE INVENTION The present invention has been proposed in order to solve the above-mentioned problems, and it is an object of the present invention to provide a method of replacing a chemical additive harmful to human body such as a synthetic surfactant, artificial coloring matter and animal- It is suitable for use in infants, sensitive skin and skin patients, and aims to improve the detergency and antibacterial power of existing handwashes.

In addition, it is intended to improve the soothing, whitening, deodorizing and moisturizing effect of the skin by adding a raw material derived from natural sources such as bamboo, gyeonggyeong, aloe and chanthoxylum extract to the mixture before lactic acid fermentation.

In order to accomplish the above object, the present invention provides a hand wash composition using a fermentation broth comprising a fermented lactic acid bacterium obtained by inoculating a fermented lactic acid bacterium in a broth containing fermented broth separated from embryogenic broth, 5 to 10% by weight of the fermentation broth and 90 to 95% by weight of the cleaning composition are composed of the active ingredient.

The mixture before lactic acid fermentation may be composed of 85 to 90% by weight of purified water, 2 to 5% by weight of EM (Effective Microorganism) activity, 2 to 4% by weight of molasses, 1 to 3% by weight of sugar, and 1 to 3% .

The lactic acid bacteria pre-fermentation mixture and distilled juice are added at a weight ratio of 5: 1, stirred and fermented at 32 to 37 ° C for 45 to 47 days.

The lactic acid fermentation broth has antimicrobial activity from E. coli and Staphylococcus aureus, and is excellent in skin soothing effect and moisturizing effect.

The cleaning composition comprises 62 to 66% by weight of purified water, 6 to 8% by weight of sodium cocoyl apple amino acid, 6 to 8% by weight of coco betaine, 3 to 5% by weight of glycerin, 3 to 4.5% 3 to 4.5 wt% of Ganoderma lucidum extract, 3 to 4.5 wt% of Ganoderma lucidum, 1 to 3 wt% of butylene glycol, 0.4 to 0.6 wt% of 1,2 hexanediol, 0.4 to 0.6 wt% of allantoin, 0.3 to 1 wt% , And 0.05 to 0.15% by weight of tea tree leaf oil.

The ginseng extract may be obtained by using purified water, ethanol, butylene glycol, or a mixed solvent thereof.

The in-line extract may be obtained by using purified water, ethanol, butylene glycol, or a mixed solvent thereof.

As described above, the hand wash composition according to the present invention can be used as a synthetic surfactant contained in conventional handwashes, such as sodium lauryl sulfate and sodium laureth sulfate, a plant-derived natural surfactant such as sodium cocoyl apple amino acid and cocoa betaine It has an effect suitable for cleansing in the hands of infants, skin patients and sensitive skin people.

In addition, the natural surfactant is added to the fermentation broth of lactic acid bacteria, and the antioxidative effect of the activated lactic acid bacteria is excellent for removing the active oxygen of the skin, and the antibacterial activity against the E. coli and the Staphylococcus aureus pathogens And when the washed sewage flows into the natural environment, the biodegradability of the lactic acid fermentation broth is degraded by the microorganisms, thereby reducing the water pollution, and there is an environment-friendly effect, and there is no organic solvent, It is harmless to the human body.

In addition, natural surfactant and fermentation broth of lactic acid bacteria are added as an effective ingredient and natural raw materials such as pear, gyeonggyeong, aloe, and clove extract are added in order to improve the washing power of the hands, and also to improve antibacterial, sedative, whitening, .

FIG. 1 is an experimental photograph showing the washing power of a hand wash composition using a fermentation broth of lactic acid bacteria prepared by applying cosmetics to skin.
FIG. 2 is a micrograph showing the application of fine dust to nude mice similar to the skin, enlarging the nude mouse skin,
FIG. 3 is a photograph showing the micrograph of the nude mouse skin after applying fine dust on the skin of the nude mouse and washing it with a hand wash composition using a fermented broth and a lactic acid fermentation broth,
FIG. 4 is a photograph showing a micrograph of an enlarged front and rear of a skin after applying fine dust to the skin of a nude mouse, washing it with a third product,
FIG. 5 is a photograph showing a micrograph of a nude mouse applied with fine dust on its skin, cleansed with purified water,
FIG. 6 is a photograph of a phase contrast microscope obtained by inoculating lactic acid bacteria into a medium containing a banana, tomato, apple, and embryo juice, and observing the growth state.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, a hand wash composition using a fermentation broth of lactic acid bacteria according to the present invention will be described in detail with reference to the accompanying drawings.

The Applicant has found that lactic acid bacteria have antimicrobial activity against E. coli and Staphylococcus aureus strains with skin protection, antioxidative effect, skin soothing effect, moisturizing effect, and particularly, effective antibacterial activity (Effective Micro-organism) Based on the results of studies that have the effect of strengthening the skin, it was decided to proceed the experiment to effectively propagate the beneficial lactic acid bacteria to the skin and apply it as an effective ingredient of the hand wash composition.

The above-described hand wash composition largely consists of 5 to 10% by weight of the lactic acid fermentation broth and 90 to 95% by weight of the cleaning composition as active ingredients.

The mixture before fermentation with lactic acid bacteria is composed of 85 to 90% by weight of purified water, 2 to 5% by weight of EM (Effective Microorganism) activity, 2 to 4% by weight of molasses, 1 to 3% by weight of sugar and 1 to 3% by weight of sun salt.

Lactic acid bacteria of the EM (Effective Microorganism) activity added to the mixture before lactic acid bacteria fermentation may be selected from the group consisting of Lactobacillus acidophilus, Lactobacillus rhamnosus and Bifidobacterium bifidum, , And the experiment was conducted by selecting the same LOT number and pH 3.37 lactobacillus based on the test report in order to confirm that it is an activated lactic acid bacteria suitable for the experiment. I decided to do it.

Experimental Example 1 [Observation of lactic acid bacterial activity according to fermentation additives]

1. Experimental Method

In order to observe the activity of the lactic acid bacteria in detail in this Experimental Example 1, the photographs were taken using a phase difference microscope (Iponacology Corp., ODEO-2222), and the magnification of the microscope was set at 17X magnification 10X: 1,080X, 2X: 2,161X, Microscope slides, forceps, cutter knives, tweezers, and sterile swabs.

2. Experimental Procedure

The fermentation additive is to be used as food for lactobacillus. It is used to wash banana, tomato, apple, and pear respectively. Each banana, tomato, apple, and pear is pressurized and juiced so that the lactobacillus can be effectively cultured. .

Fermentation was carried out for 37 days at 37 ° C (temperature based on liquid) for a maximum of 47 days on a stainless steel and teflon fermentation mixed tank at a ratio of 5: 1 by weight before fermentation with lactic acid bacteria fermentation.

3. Experimental Results

Table 1 below shows the results of measuring the activity of lactic acid bacteria according to different fermentation additives for a maximum of 47 days at intervals of 15 days and measuring the number of lactic acid bacteria.

May 24 (early days)

June 8 (around the 15th)
June 23 (around the 30th)
July 10 (around the 47th)
banana

1.2 × 10 ⁶ CFU / mL
2.1 x 10 < ⁶ > CFU / mL
3.4 × 10 ⁶ CFU / mL
5.6 x 10 ⁶ CFU / mL
tomato

1.2 × 10 ⁶ CFU / mL
2.2 x 10 < ⁶ > CFU / mL
3.2 x 10 < ⁶ > CFU / mL
6.8 x 10 ⁶ CFU / mL
Apple

1.2 × 10 ⁶ CFU / mL
3.2 x 10 < ⁶ > CFU / mL
4.1 × 10 ⁶ CFU / mL
6.1 × 10 ⁶ CFU / mL
ship

1.3 x 10 < ⁶ > CFU / mL
3.5 x 10 < ⁶ > CFU / mL
5.2 × 10 ⁶ CFU / mL
8.2 x 10 < ⁶ > CFU / mL

As shown in Table 1 above, fermentation for 47 days resulted in a larger number of lactic acid bacteria in the order of pear, tomato, apple, and banana. At this time, as shown in FIG. 4, the number of viable cells was the largest at 8.2 × 10 ⁶ CFU / mL, and actinomycetes were actively observed.

 Therefore, in the present invention, it was decided to use the fermentation additive as the bait according to the result of Experiment 1, and lactic acid bacteria were inoculated into the culture medium containing the nutrient juice separated from the fermentation broth using Naju's special product, Fermented and added to the cleaning composition, and a cleaning experiment was conducted in Experiment 2.

Experimental Example 2 [Experiment of fine dust cleaning of nude mice]

Experimental Example 2 is an experiment for evaluating detergency by applying fine dust to a nude mouse having a skin-like surface by using the hand wash composition of the present invention and washing the same.

1. Experimental Method

The fine dust was put into a high-temperature high-pressure sterilizer and sterilized at 121 ° C for 15 minutes. A small amount of fine dust was removed with a cotton swab and applied to the skin surface of nude mice.

2. Experimental apparatus and materials

The present invention hand wash products, third party hand wash products, fine dust, nude mice. (DZ-1D, ~ 500g max), Sterile, Test tube diameter 12mm, Purified water production device (Daion Science, LIMITE400), High temperature high pressure sterilizer (Dong-A Science, DA-4C ).

The cleaning composition of the hand wash product according to the present invention comprises 62 to 66% by weight of purified water, 6 to 8% by weight of sodium cocoyl apple amino acid, 6 to 8% by weight of coco betaine, 3 to 5% by weight of glycerin, To 4 wt.% Of atelocollagen, 3 to 4.5 wt.% Of Centella asiatica extract, 3 to 4.5 wt.% Of Ginkgo extract, 1 to 3 wt.% Of butylene glycol, 0.4 to 0.6 wt.% Of 1,2 hexanediol, 0.3 to 1% by weight of lemon oil, and 0.05 to 0.15% by weight of tea tree leaf oil.

The third party hand wash product may be selected from the group consisting of purified water, glycerin, lauric acid, potassium azide, myristic acid, stearic-11, laurylbetaine, ethanolamine, propylene glycol, tetrasodium dimethylacetate, Tylene, butylene glycol, and perfume.

3. Experimental Procedure

(1) Draw a 20 x 20 mm square on the surface of the nude mouse and clean the surface cleanly.

(2) Observe whether the surface has been cleaned or cleaned with a microscope. In this case, observe various parts to confirm that the surface is uniform.

(3) Experiment the above procedure with purified water (test), developed product, and control sample, respectively.

4. Experimental Formula

Assuming that the coated area of the nude mouse skin surface is divided by 10 * 10 and the applied area is 100, check the area after washing with a given sample.

Calculation formula: (100 - Residual area of fine dust)

----------------------------------------- × 100%

Total fine dust application area

5. Experimental results

(1) Fine dust cleaning ability of the hand wash product of the present invention

As shown in FIG. 3, the area of the initial fine dust applied was 100, and the area cleaned after washing with the pumping amount of the hand cleaner No. 1 was 100%.

(2) Fine dust cleaning ability of other hand wash products

As shown in FIG. 3, the area of the initial fine dust applied was 100, and the cleaned area after washing with the pumping amount of the hand cleaner No. 1 was 89%.

(3) Purified water fine dust cleaning power

The area of initial fine dust applied was 100, and the area cleaned after washing with purified water was 40%.

Therefore, the cleaning effect of the hand wash product, the third party hand wash product, and the purification procedure of the present invention was found as a result of the fine dust cleaning test of the skin surface of the nude mouse, and the hand wash product of the present invention had a cleaning power of 100% Respectively.

Experimental Example 3 [Antibacterial Experiment]

Experimental Example 3 was conducted in order to evaluate the antimicrobial effect of the present sample (hand wash composition) according to the test standard ASTM E2315-16.

1. Experimental Method

Experiments were carried out by inoculating the test strains E. coli (Gram negaitive. ATCC 25922) and S. aureus (Gram positive, ATCC 6538).

The media and reagents used were Brain heart infusion broth (DIFCO, USA), Tryptic soy agar (DIFCO, USA), D / E neutralizing broth (DIFCO, USA) and sterile physiological saline.

The experimental strain is inoculated into the present sample (hand wash composition) and cultured at 34 to 36 DEG C for 18 to 24 hours.

2. Preparation of experimental bacteria

The cultured broth was transferred to a fresh medium and cultured. The broth was diluted in sterilized saline to a concentration of 1 to 9.9 10 ⁸ CFU / mL and used as an experimental broth.

3. Experimental Procedure

(1) To 20 mL of the present sample (hand wash composition), 0.2 mL of the test bacterial solution was added, mixed, and left at room temperature for 3 minutes.

(2) Initial dilution was carried out using a D / E neutralizing broth.

(3) The neutralized test solution was diluted step by step and dispensed at 1 mL per 2 Petri dishes.

(4) Prepared Tryptic soy agar at 45 to 50 ° C was dispensed in a Petri dish at 15 to 25 mL, and solidified at room temperature.

(5) The solidified Petri dish was inverted and cultured at 35 DEG C for 24 to 48 hours.

(6) The experiment was repeated twice for each strain, and the number of the live cells was measured using sterile physiological saline solution.

4. Experimental Formula

(1) Number of live cells

N = C x D

N: number of viable cells, C: number of colonies (average number of two colonies), D:

(2) Reduction rate

LR = mean log (microbial population) - mean log (surviving test population)

5. Experimental results

division After 3 minutes Number of living cells (CFU / mL) Log value mean log
Control (initial)
5.6 × 10 ⁶

6.75
6.75
Test group 1
<20

<1.30

<1.30

Test group 2

<20
<1.30

As shown in Table 2, E. coli was found to have an initial number of inoculated bacteria of 5.6 × 10 ⁶ CFU / mL, and after 3 minutes, the number of bacteria was observed to be <20 CFU / mL in two repeated tests.

division After 3 minutes Number of living cells (CFU / mL) Log value mean log
Control (initial)
2.4 × 10 ⁶

6.38
6.38
Test group 1
<20

<1.30

<1.30

Test group 2

<20
<1.30

As a result of the antibacterial activity of S. aureus, the initial number of inoculated bacteria was 2.4 × 10 ⁶ CFU / mL, and after 3 minutes, the number of bacteria was observed to be 20 CFU / mL in two repeated tests.

division
Number of living cells (CFU / mL)
After 3 minutes Average number of live bacteria LR * (log reduction) E coli 6.75 <1.30 > 5.45 S. aureus 6.38 1.30 5.08

As shown in Table 4, the log reduction values after 3 minutes for the samples (hand wash composition) under the test conditions were> 5.45 and 5.08 for E coli and S. aureus, respectively.

Log reduction Percent (%) log reduction 1 or more over 90 2 or more 99% or more 3 or more 99.9% or more 4 or more 99.99% or more 5 or more 99.999% or more

Table 5 is an interpretation of the results. Referring to Table 5, the antibacterial activity of the hand wash composition according to the present invention was found to be 99.999% or more, as shown in Table 4 below.

Therefore, the present invention has the effect of completely washing micro-dusts from the hands while antibacterial microbes in the micro-dust can be perfectly cleaned with the fermentation broth of lactic acid bacteria utilizing Naju, which is a special product of this region.

In addition, synthetic surfactants such as sodium lauryl sulphate and laureth sulphate, which are contained in conventional handwashes, have been replaced by vegetable natural surfactants such as sodium cocoyl apple amino acid extracted from plants and cocoa bean, It has an effective effect to be cleansed by hand. It has natural surfactant and lactic acid fermentation broth as an effective ingredient and additionally added natural raw materials such as pear, gyeonggyeong, aloe, , Whitening, irritation, deodorization and moisturizing effect.

The present invention may be embodied in many other forms without departing from the spirit or essential characteristics thereof. The foregoing embodiments are merely illustrative in all respects and should not be construed as being limited by what is described in the detailed description of the specification. Modifications and variations that fall within the equivalent range of the claims are all within the scope of the present invention.

Claims (7)

5 to 10% by weight of a lactic acid fermentation broth fermented by inoculating a fermented lactic acid bacterium in a medium or culture containing the fermentation broth obtained by fermenting the fermented broth and the fermented broth, and 90 to 95% by weight of the cleaning composition,
The cleaning composition comprises 62 to 66% by weight of purified water, 6 to 8% by weight of sodium cocoyl apple amino acid, 6 to 8% by weight of coco betaine, 3 to 5% by weight of glycerin, 3 to 4.5% 3 to 4.5 wt% of Ganoderma lucidum extract, 3 to 4.5 wt% of Ganoderma lucidum, 1 to 3 wt% of butylene glycol, 0.4 to 0.6 wt% of 1,2 hexanediol, 0.4 to 0.6 wt% of allantoin, 0.3 to 1 wt% , And 0.05 to 0.15% by weight of tea tree leaf oil. The method according to claim 1,
Characterized in that the mixture before fermentation with lactic acid bacteria is composed of 85 to 90% by weight of purified water, 2 to 5% by weight of EM (Effective Microorganism) activity, 2 to 4% by weight of molasses, 1 to 3% by weight of sugar, and 1 to 3% And a hand wash composition using the lactic acid fermentation broth. 3. The method of claim 2,
Wherein the fermentation broth is fermented at 32 to 37 DEG C for 45 to 47 days by adding the pre-fermentation broth and the pre-fermentation broth at a ratio of 5: 1 by weight and stirring. delete delete The method according to claim 1,
Wherein the extract of Ganoderma lucidum is obtained from purified water, ethanol, butylene glycol, or a mixed solvent thereof. The method according to claim 1,
Wherein the centilla extract is obtained by using purified water, ethanol, butylene glycol, or a mixed solvent thereof, and a hand wash composition using the fermented broth of lactic acid bacteria.

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