CN102793654A - Cleansing milk - Google Patents
Cleansing milk Download PDFInfo
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- CN102793654A CN102793654A CN2012102133776A CN201210213377A CN102793654A CN 102793654 A CN102793654 A CN 102793654A CN 2012102133776 A CN2012102133776 A CN 2012102133776A CN 201210213377 A CN201210213377 A CN 201210213377A CN 102793654 A CN102793654 A CN 102793654A
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Abstract
The invention discloses cleansing milk. The cleansing milk comprises a surfactant, a humectant, a nutrient, an emollient, an essence and a bacteriacide, and is characterized in that the bacteriacide is bacillus subtilis (CGMCC 6012) fermentation liquor extract. By the cleansing mile, the dirt on the skin can be removed; moreover, the cleansing milk has good sterilization and disinfection functions; and particularly, the invention is characterized in that the cleansing milk can kill methicillin-resistant staphylococcus aureus. The bacteriacide which is added into the cleansing milk is produced by using fermentation raw materials with low cost and applying a modern biotechnology, the process is simple, and the cost is low. Therefore, the cleansing milk has high bactericidal power and technical content, and is safe and cheap. In addition, the bacteriacide which is added into the cleansing milk and is obtained by processing pure natural fermentation raw materials by the modern biotechnology also has an anticorrosive function, and has the characteristics of safety and high efficiency.
Description
Technical field: the present invention relates to field of hygiene products, particularly relate to a kind of special cleansing milk.
Background technology:
Cleansing milk is exactly to adopt the mode of washing to clean the emulsion form product of human body face.The basis of cleansing milk at present commonly used is water and surfactant.Add additives such as essence, wetting agent, antiseptic, antioxidant with stable prod, give its fragrance.
People's perspiration has formed dirt with scurf of dust in air and human body etc.On dirt, assembled a large amount of microorganisms, some are wherein just arranged possibly be the source of disease microorganism, and these health to people are brought certain threat.Yet,, can not kill the facial antibacterial of people effectively though the most cleansing milk in market can play good cleaning effect.Facial antibacterial can cause people to form acne, also might cause some diseases simultaneously.In the cleansing milk, generally add antiseptic and prevent the putrid and deteriorated of bath gel at present, these antiseptic have certain danger to human beings'health, simultaneously skin are had certain stimulation.
Summary of the invention:
The purpose of this invention is to provide a kind of highly effective and safe, adopt the cleansing milk product of modern biotechnology product processing.
Prescription of the present invention mainly contains: surfactant, wetting agent, nutrient, emollient, essence, antibacterial.
Major technique characteristic of the present invention be added have inexpensive, highly effective and safe, all-natural is through the antibacterial of modern biotechnology processing.Antibacterial is bacillus subtilis (Bacillus subtilis) fermentation broth extract; This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 16th, 2012; The preservation address is positioned at the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3; (postcode 100101), deposit number are CGMCCNo.6012.
Fungus characteristic is following:
1. somatic cells is life or the living Gram-positive of near-end in direct rod shape, the spore.
2. colony characteristics is that white is circular, protuberance, and smooth surface, opaque, moistening, provoke adhesion.
3. can utilize various saccharides, like glucose, galactose, xylose, cellobiose, cottonseed sugar etc.; Produce amylase, protease, can gelatin hydrolysate, V.P, C.I. 13020. are positive.
4. 37 ℃ of the righttest cultivation temperature, optimum pH 7.0.
This bacterium classification status is following: antibacterial territory (Bacteria); Firmicutes (Firmicutes); Bacillus cereus guiding principle (Bacillales); Bacillus cereus order (Bacillaceae); Bacillaceae (Bacillus); Bacillus (Bacillus); Bacillus subtilis strain (Bacillus subtilis).
The method for preparing of fermentation broth extract is following among the present invention:
The seed culture medium of bacterial strain is a broth bouillon, Carnis Bovis seu Bubali cream 2g wherein, peptone 8g, NaCl 5g, water 1000ml, pH value 7.0 ~ 7.2, high pressure steam sterilization.
The seed liquor condition of culture: cultivation temperature is 33 ℃, and shaking speed is 140 rev/mins, and fermentation time is 13 hours.
Inoculum concentration is 8%, helps the formation of antibacterial substance.
The fermentation fermentation medium consists of: glucose 0.3%, and peptone 1%, NaCl 0.5%, MgSO
4.7H
2O0.7%, KH
2PO
40.7%, pH8.0.
Fermentation culture conditions is: cultivation temperature is 33 ℃, and shaking speed is 140 rev/mins, and fermentation time is about 24 hours.
From fermentation liquid, extracting the antibacterial substance method is organic solvent precipitation method.
At first with the fermentation liquid high speed centrifugation, temperature 0-4 ℃, rotating speed is 8000 rev/mins, centrifugal ten minutes, keeps supernatant.
(methanol: ethanol=1:6), fully dissolving is placed in the there-necked flask, and 40 degrees centigrade of temperature are controlled in water-bath, and mixing speed is 60rpm, and the time is 5 hours, collects organic facies, adopts vacuum distillation method to remove organic solvent to add the equal-volume organic solvent in the supernatant.Vapo(u)rizing temperature 30-60 ℃, distilled 20 minutes, organic solvent is removed.The collecting precipitation material.Promptly get antibacterial.
The crude extract of Fungicidal substance, behind water dissolution, concentration is 1% (mass volume ratio).Lysate can kill common pathogenic bacterium effectively.For example not only can kill escherichia coli effectively, Salmonella, the pathogenic microorganism that golden yellow common coccus etc. are common, and can kill the common coccus of methicillin-resistant staphylococcus.
This bactericidal liquid has high safety; In the emergency toxicology experiment, we irritate 5 groups of 200 mouse of clothes with this bactericidal liquid, and the mouse of experimental group none occur dead; And bacillus subtilis is present in occurring in nature widely, and many important industrial bacterial strains are exactly bacillus subtilis.Basically can conclude that this bactericidal liquid is safe for user, can relievedly use.After adding it to cleansing milk, find that it still has good bactericidal activity.
Method for making of the present invention is simple; With deionized water, surfactant (5 ~ 20%), wetting agent (3 ~ 6%), nutrient (0.5 ~ 3%), emollient (0.5 ~ 3%), antibacterial (0.1 ~ 1%) and essence; Join in the agitated reactor; Stir, regulate PH, can obtain cleansing cream with high-efficiency sterilization through the degassing, fill.The adding proportion of each material is the percentage ratio of gross weight.
Concrete technology is following: deionized water is added in the agitated reactor, its temperature is heated to 70 ℃, add the cocamido propyl betaine of regulation ratio then successively, trialkyl methyl oxidation amine; Coconut monoethanolamide, glycerol, water-soluble modified silicone oil stirs, and speed of agitator is unsuitable too high; Can be controlled in 20 ~ 30 rev/mins, cool the temperature to 40 ℃, the pH value of solution is transferred to 6.5-7.0, add collagen protein with citric acid; Antibacterial, essence, insufficient section stirs with the deionized water polishing, and speed of agitator can be controlled in 20 ~ 30 rev/mins; The feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removes the bubble that in the stirring and dissolving process, forms; Reduce the air content of material, mixed liquor is carried out packing, can obtain cleansing milk.
Surfactant, this product preferred surfactants has fatty alcohol-polyoxyethylene ether, Oleum Cocois single ethanol amide, cocamido propyl betaine, one or more in cocamidopropyl propyl amide amine oxide, the trialkyl methyl oxidation amine etc.
Glycerol can prevent that water content reduces in the skin, and can improve the tolerance to cold of product as wetting agent, the variation that can not produce form when product is used.
Nutrient is a collagen protein.
Emollient comprises water-soluble modified silicone oil, cation-modified silicone oil, one or more of amino-modified silicone oil.
Citric acid is an amount of, is used to regulate pH value.
Used essence can add according to the odor type of product, makes product have different odor types, satisfies the needs of different consumers.
Beneficial effect:
The present invention has not only realized the dirt on the cleansing milk removal skin, and has possessed good antibacterial and disinfectant function, particularly can kill the characteristic of methicillin-resistant staphylococcus aureus.The emergency toxicology experiment has also verified the antibacterial that adds in this cleansing milk people and animals to be had very high safety.Scientist has found methicillin-resistant staphylococcus aureus in 1961, find that at present it spreads all over the world, and becomes important infectious bacteria, has brought great threat for people's health.The production of the antibacterial that adds in the cleansing milk utilizes fermentation raw material with low cost, adopts modern biotechnology, and technology is simple, and is with low cost.Make the present invention, it is strong to become a kind of sterilizing power, safety, cheapness, cleansing milk with high content of technology.
That adds among the present invention has the antiseptical function by the pure natural fermentation raw material through the antibacterial that modern biotechnology processes, and has characteristics safely and efficiently.Need not to add again antiseptic in the cleansing milk.
Specific embodiment:
Below in conjunction with specific embodiment the present invention is described further, but is not limited to embodiment.
Induction mutation of bacterium
Take the ultraviolet mutagenesis method: with CICC10023 is starting strain, processes bacteria suspension, coats on the culture medium plate, cultivates in 37 ℃ of incubators behind the ultra-vioket radiation 90s.
Bacterial screening
Choose 30 single bacterium colonies of looking strong from the 90s plate, be inoculated on the inclined-plane, in 37 ℃ of incubators, cultivated 0--4 ℃ of stored refrigerated 24 hours; With original strain as contrast; Adopt conventional selection-breeding condition; Sodium carboxymethyl cellulose is as carbon source and Proteolytic enzyme circle method screening cellulase-producing and the strong bacterial strain of protease; Screening obtains cellulase-producing and strong bacterial strain bacillus subtilis (Bacillus subtilis) L1 of protease, and this bacterial strain cellulase-producing can improve 25% by the force rate original strain, produces protease and can improve 52% by the force rate original strain.In the common micro-organisms center preservation of Chinese common micro-organisms culture presevation administration committee, deposit number is CGMCC No 6012 with this strain.
The used bacillus subtilis of this product (Bacillus subtilis) strain; This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 16th, 2012; The preservation address is positioned at the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3; (postcode 100101), deposit number are CGMCC No6012.
Show through experimentation: antibacterial have good inhibition escherichia coli, staphylococcus aureus, etc. the characteristics of pathogenic bacterium.
Embodiment 1, preparation 100kg product.
The 50kg deionized water is added in the agitated reactor, its temperature is heated to 70 ℃, add the 5kg cocamido propyl betaine then successively, 2kg trialkyl methyl oxidation amine; The 2kg coconut monoethanolamide, 4kg glycerol adds the water-soluble modified silicone oil of 3kg then and stirs, and speed of agitator is unsuitable too high; Can be controlled in 25 rev/mins, cool the temperature to 40 ℃, the pH value of solution is transferred to 6.5, collagen protein 1kg with citric acid; Add the 0.2kg antibacterial, 0.001kg essence, insufficient section stirs with the deionized water polishing, and speed of agitator can be controlled in 25 rev/mins; The feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removes the bubble that in the stirring and dissolving process, forms; Reduce the air content of material, mixed liquor is carried out packing, can obtain washing milk.
Get 10% concentration product of the present invention, adopt conventional microorganism detection bactericidal effect, result of the test shows that product bactericidal effect of the present invention reaches more than 90%, and bactericidal effect is obvious.Escherichia coli, Salmonella, golden yellow common coccus are selected in microorganism.
Claims (5)
1. a cleansing milk comprises surfactant, wetting agent, nutrient, emollient, essence, antibacterial, it is characterized in that, said antibacterial is bacillus subtilis CGMCC 6012 fermentation broth extracts.
2. according to the said cleansing milk of claim 1, the percentage by weight that it is characterized in that said antibacterial is 0.1 ~ 1%.
3. a method for preparing the said cleansing milk of claim 1 is characterized in that, this method for preparing is following: deionized water is added in the agitated reactor; Be heated to 70 ℃, add percentage by weight and be respectively 5 ~ 20% surfactant, 20 ~ 30 rev/mins of 3 ~ 6% wetting agents, 0.5 ~ 3% emollient, speed of agitator; Be cooled to 40 ℃; PH value transfers to 6.5-7.0, and the adding percentage by weight is 0.5 ~ 3% nutrient, the antibacterial of 0.1-1%, essence, and insufficient section is used the deionized water polishing; Stir the degassing, fill.
4. according to the method for preparing of the said cleansing milk of claim 3, it is characterized in that said surfactant is at least a in fatty alcohol-polyoxyethylene ether, Oleum Cocois single ethanol amide, cocamido propyl betaine, cocamidopropyl propyl amide amine oxide or the trialkyl methyl oxidation amine.
5. according to the method for preparing of the said cleansing milk of claim 3, it is characterized in that said emollient is at least a in water-soluble modified silicone oil, cation-modified silicone oil or the amino-modified silicone oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102133776A CN102793654A (en) | 2012-06-24 | 2012-06-24 | Cleansing milk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102133776A CN102793654A (en) | 2012-06-24 | 2012-06-24 | Cleansing milk |
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| Publication Number | Publication Date |
|---|---|
| CN102793654A true CN102793654A (en) | 2012-11-28 |
Family
ID=47193127
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012102133776A Pending CN102793654A (en) | 2012-06-24 | 2012-06-24 | Cleansing milk |
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|---|---|
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104586741A (en) * | 2015-02-05 | 2015-05-06 | 上海昊淼生物科技有限公司 | Bubble enzyme facial mask |
| CN104644490A (en) * | 2014-06-10 | 2015-05-27 | 莫治玲 | Facial cleaner |
| CN112516068A (en) * | 2020-12-15 | 2021-03-19 | 福建洛东生物技术有限公司 | Application of bacillus subtilis in antibacterial, deodorizing and cleaning products |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507700A (en) * | 2008-03-12 | 2009-08-19 | 北京绿岛缘蜂产品有限公司 | High-efficiency sterilization shower cream |
| CN101507702A (en) * | 2008-03-12 | 2009-08-19 | 北京绿岛缘蜂产品有限公司 | High-efficiency sterilization cleansing facial milk |
-
2012
- 2012-06-24 CN CN2012102133776A patent/CN102793654A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507700A (en) * | 2008-03-12 | 2009-08-19 | 北京绿岛缘蜂产品有限公司 | High-efficiency sterilization shower cream |
| CN101507702A (en) * | 2008-03-12 | 2009-08-19 | 北京绿岛缘蜂产品有限公司 | High-efficiency sterilization cleansing facial milk |
Non-Patent Citations (1)
| Title |
|---|
| 白雪: "水嫩冬季", 《现代养生》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104644490A (en) * | 2014-06-10 | 2015-05-27 | 莫治玲 | Facial cleaner |
| CN104586741A (en) * | 2015-02-05 | 2015-05-06 | 上海昊淼生物科技有限公司 | Bubble enzyme facial mask |
| CN104586741B (en) * | 2015-02-05 | 2018-09-11 | 上海昊淼生物科技有限公司 | A kind of bubble ferment facial mask |
| CN112516068A (en) * | 2020-12-15 | 2021-03-19 | 福建洛东生物技术有限公司 | Application of bacillus subtilis in antibacterial, deodorizing and cleaning products |
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Application publication date: 20121128 |
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