CN103417460A - Shampoo - Google Patents

Abstract

The invention discloses an efficient sterilizing shampoo, which belongs to the field of hygienic products, relates to the special shampoo, and aims to provide the efficient and safe sterilizing shampoo product processed by adopting modern biotechnology products. The ingredient of the shampoo mainly comprises surfactant, foam booster, tackifier, conditioner, pigment, and essence. The main technical characteristics are that cheap, efficient, safe, and natural sterilizing agent being subjected to modern biotechnology process is added. The added sterilizing agent manufactured by the modern biotechnology process of pure natural fermentation raw materials has the function of anticorrosion, and has the characteristics of safety and high efficiency; therefore, preservative is not required to be added to the shampoo.

Description

A kind of shampoo

Technical field: the invention belongs to field of hygiene products, relate to special shampoo.

Background technology:

At sex pheromones such as hospitals, assemble in more environment, sex pheromone also can be gathered on hair.Dust on hair and dirt are the good environments of microbial growth, especially the more suitable microbial growth of root of hair temperature and humidity.Once being attached on hair, sex pheromone just likely obtains a large amount of breedings.Shampoo can be removed the dirt of hair, can remove a certain amount of microorganism simultaneously, but our common shampoo does not possess sterilizing function, so can't thoroughly remove and pathogenic microbe killing.Therefore may bring threat to our health.

Shampoo in the market seldom has sterilizing function.In shampoo, generally add antiseptic and prevent the putrid and deteriorated of shampoo at present, these antiseptic have certain danger to the mankind's health, may certain stimulation be arranged to skin simultaneously.

Summary of the invention:

The purpose of this invention is to provide a kind of highly effective and safe, adopt the sterilization shampoo product of modern biotechnology product processing.

Product composition of the present invention comprises deionized water, polyethylene glycol stearate diester, conditioner, suds booster, surfactant, antibacterial, essence.

Technical characteristics of the present invention be added have inexpensive, highly effective and safe, the antibacterial through modern biotechnology processing of All Pure Nature.Antibacterial is bacillus subtilis (Bacillus subtilis) fermentation broth extract, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 16th, 2012, the preservation address is positioned at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3, (postcode 100101), deposit number is CGMCCNo.6012.

Fungus characteristic is as follows:

1. somatic cells is Gram-positive living in direct rod shape, spore or that near-end is given birth to.

2. colony characteristics is that white is circular, protuberance, and smooth surface, opaque, moistening, provoke adhesion.

3. can utilize various saccharides, as glucose, galactose, xylose, cellobiose, cottonseed sugar etc.; Produce amylase, protease, can gelatin hydrolysate, V.P, the C.I. 13020. positive.

4. the suitableeest cultivation temperature is 37 ℃, optimum pH 7.0.

This bacterium classification status is as follows: antibacterial territory (Bacteria); Firmicutes (Firmicutes); Bacillus cereus guiding principle (Bacillales); Bacillus cereus order (Bacillaceae); Bacillaceae (Bacillus); Bacillus (Bacillus); Bacillus subtilis strain (Bacillus subtilis).

In the present invention, the preparation method of fermentation broth extract is as follows:

The seed culture medium of bacterial strain is broth bouillon, Carnis Bovis seu Bubali cream 2g wherein, peptone 8g, NaCl 5g, water 1000ml, pH value 7.0～7.2, high pressure steam sterilization.

The seed liquor condition of culture: cultivation temperature is 33 ℃, and shaking speed is 140 rev/mins, and fermentation time is 13 hours.

Inoculum concentration is 8%, is conducive to the formation of antibacterial substance.

The fermentation fermentation medium consists of: glucose 0.3%, and peptone 1%, NaCl 0.5%, MgSO ₄.7H ₂O0.7%, KH ₂PO ₄0.7%, pH8.0.

Fermentation culture conditions is: cultivation temperature is 33 ℃, and shaking speed is 140 rev/mins, and fermentation time is about 24 hours.

Extracting the antibacterial substance method from fermentation liquid is organic solvent precipitation method.

At first by the fermentation liquid high speed centrifugation, temperature 0-4 ℃, rotating speed is 8000 rev/mins, centrifugal ten minutes, retains supernatant.

(methanol: ethanol=1:6), fully dissolve and be placed in there-necked flask, 40 degrees centigrade of temperature are controlled in water-bath, and mixing speed is 60rpm, and the time is 5 hours, collect organic facies, adopt vacuum distillation method to remove organic solvent to add the equal-volume organic solvent in supernatant.Vapo(u)rizing temperature 30-60 ℃, distill 20 minutes, and organic solvent is removed.The collecting precipitation material.Obtain antibacterial.

The crude extract of Fungicidal substance, with after water dissolution, concentration is the 1%(mass volume ratio).Lysate can kill common pathogenic bacterium effectively.For example not only can effectively kill escherichia coli, Salmonella, the pathogenic microorganism that golden yellow common coccus etc. are common, and can kill the common coccus of methicillin-resistant staphylococcus.

This bactericidal liquid has high safety, in the emergency toxicology experiment, we gavage 5 groups of 200 mouse by this bactericidal liquid, and the mouse of experimental group none occur dead, and bacillus subtilis is present in occurring in nature widely, many important industrial bacterial strains are exactly bacillus subtilis.Substantially can conclude that this bactericidal liquid is safe for user, can relievedly use.After adding it to shampoo, find that it still has good bactericidal activity.

Method for making of the present invention is simple, deionized water is added in reactor, its temperature is heated to 70 ℃, add successively polyethylene glycol stearate diester (1～5%), conditioner (1～5%), suds booster (3～10%) is stirred to dissolving, then add surfactant (9～20%) to stir, speed of agitator is unsuitable too high, can be controlled in 20～30 rev/mins, cool the temperature to 40 ℃, with citric acid, the pH value of solution is adjusted to 6.5～7.0, the essence that adds antibacterial (0.1～1%) and 0.0001-0.0005%, stir, speed of agitator can be controlled in 20～30 rev/mins, by the feed liquid be stirred, undertaken degassed, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, the bubble that removal forms in the stirring and dissolving process, reduce the air content of material, mixed liquor is carried out to packing, can obtain shampoo.Ratio accounts for the percentage by weight of product gross weight for each component herein.

The surfactant that the present invention adds is: dodecanol sodium sulfate; Sodium Alcohol Ether Sulphate etc.

Suds booster is coconut monoethanolamide, cocoanut oil diethan olamide etc.Can play foam enhancing, the effect of steady bubble, tackify.

Select polyquaternium-7 for conditioner.

Citric acid is appropriate, for regulating pH value.

All the other compositions are deionized water.Essence used can add according to the odor type of product, makes product have different odor types, meets the needs of different consumers.

Beneficial effect:

The present invention, not only realized that people remove the dirt on hair, makes straight smooth, the anti-dandruff function of hair, and possessed the function of good sterilization and sterilization, particularly can kill the characteristic of methicillin-resistant staphylococcus aureus.The emergency toxicology experiment has also verified the antibacterial added in this fancy soap people and animals to be had to very high safety.Scientist has found methicillin-resistant staphylococcus aureus in 1961, find that at present it spreads all over the world, and becomes important infectious bacteria, has brought great threat to people's health.The production of the antibacterial added in shampoo, utilize fermentation raw material with low cost, adopts modern biotechnology, and technique is simple, with low cost.Make the present invention, become a kind of hair washing effective, safety, cheapness, shampoo with high content of technology.

The antibacterial processed through modern biotechnology by the pure natural fermentation raw material added in the present invention has the antiseptical function, has characteristics safely and efficiently.In shampoo without adding again antiseptic.

Specific embodiment:

Induction mutation of bacterium

Take the ultraviolet mutagenesis method: take CICC10023 as starting strain, make bacteria suspension, coat on the culture medium plate, cultivate in 37 ℃ of incubators after ultra-vioket radiation 90s.

Bacterial screening

Choose 30 single bacterium colonies of looking strong from the 90s plate, be inoculated on inclined-plane, in 37 ℃ of incubators, cultivate 24 hours 0--4 ℃ of stored refrigerated; With original strain in contrast, adopt the conventional herd breeding condition, sodium carboxymethyl cellulose is as carbon source and Proteolytic enzyme circle method screening cellulase-producing and the strong bacterial strain of protease, screening obtains cellulase-producing and strong bacterial strain bacillus subtilis (Bacillus subtilis) L1 of protease, this bacterial strain cellulase-producing energy force rate original strain has improved 25%, produces protease energy force rate original strain and has improved 52%.By this strain, in the center preservation of Chinese common micro-organisms culture presevation administration committee's common micro-organisms, deposit number is CGMCC No 6012.

This product bacillus subtilis used (Bacillus subtilis) strain, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 16th, 2012, the preservation address is positioned at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3, (postcode 100101), deposit number is CGMCC No6012.

Research shows by experiment: antibacterial have good inhibition escherichia coli, staphylococcus aureus, etc. the characteristics of pathogenic bacterium.

Below in conjunction with specific embodiment, the invention will be further described, but be not limited to embodiment.

Embodiment 1

Prepare 100 kilograms of products, the 30kg deionized water is added in reactor, its temperature is heated to 70 ℃, add successively the 2kg polyethylene glycol stearate diester, 2kg polyquaternium-7, the 3kg coconut monoethanolamide, the 3kg cocoanut oil diethan olamide is stirred to dissolving, then adding dodecanol sodium sulfate and the 30kg concentration that 15kg concentration is 30% is that 28% Sodium Alcohol Ether Sulphate stirs, speed of agitator is unsuitable too high, can be controlled in 20～30 rev/mins, cool the temperature to 40 ℃, with citric acid, the pH value of solution is adjusted to 6.5, add the 1kg antibacterial, 0.0001kg rose essence, deionized water is supplied weight, stir, speed of agitator can be controlled in 20～30 rev/mins, by the feed liquid be stirred, undertaken degassed, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, the bubble that removal forms in the stirring and dissolving process, reduce the air content of material, mixed liquor is carried out to packing, can obtain shampoo.

Embodiment 2

Prepare 100 kilograms of products, the 30kg deionized water is added in reactor, its temperature is heated to 70 ℃, add successively the 1kg polyethylene glycol stearate diester, 1kg polyquaternium-7, the 5kg coconut monoethanolamide, adding dodecanol sodium sulfate and the 20kg concentration that 20kg concentration is 30% is that 28% Sodium Alcohol Ether Sulphate stirs, speed of agitator is unsuitable too high, can be controlled in 20～30 rev/mins, cool the temperature to 40 ℃, with citric acid, the pH value of solution is adjusted to 7.0, add the 1kg antibacterial, 0.0001kg rose essence, deionized water is supplied weight, stir, speed of agitator can be controlled in 20～30 rev/mins, by the feed liquid be stirred, undertaken degassed, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, the bubble that removal forms in the stirring and dissolving process, reduce the air content of material, mixed liquor is carried out to packing, can obtain shampoo.

Product of the present invention adopts conventional microorganism detection bactericidal effect, and result of the test shows, product of the present invention can be eliminated 90% above microorganism, and bactericidal effect is obvious.Escherichia coli, Salmonella, golden yellow common coccus are selected in microorganism.

Claims (7)

1. a shampoo, comprise polyethylene glycol stearate diester, conditioner, suds booster, surfactant, essence, it is characterized in that described shampoo contains antibacterial, and described antibacterial is bacillus subtilis CGMCC6012 fermentation broth extract.

2. shampoo according to claim 1, is characterized in that described antibacterial part by weight is 0.1～1%.

3. shampoo according to claim 1, is characterized in that described suds booster is at least one in coconut monoethanolamide, cocoanut oil diethan olamide.

4. shampoo according to claim 1, is characterized in that described surfactant is at least one in dodecanol sodium sulfate or Sodium Alcohol Ether Sulphate.

5. shampoo according to claim 1, is characterized in that described surfactant is at least one in dodecanol sodium sulfate or Sodium Alcohol Ether Sulphate.

6. shampoo according to claim 1, is characterized in that described conditioner is polyquaternium-7.

7. the preparation method of shampoo according to claim 1, its preparation method is as follows: deionized water adds in reactor, is heated to 70 ℃, the polyethylene glycol stearate diester that adds 1-5%, the suds booster of the conditioner 3-10% of 1-5%, stir, the surfactant that adds 9-20%, stir, be cooled to 40 ℃, regulate pH value to 6.5-7.0, the essence that adds 0.1-1% antibacterial and 0.0001-0.0005%, stir, degassed, packing.