CN103405378A - Hand washing solution - Google Patents
Hand washing solution Download PDFInfo
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- CN103405378A CN103405378A CN2013102864390A CN201310286439A CN103405378A CN 103405378 A CN103405378 A CN 103405378A CN 2013102864390 A CN2013102864390 A CN 2013102864390A CN 201310286439 A CN201310286439 A CN 201310286439A CN 103405378 A CN103405378 A CN 103405378A
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- washing solution
- hand washing
- handwashing liquid
- antibacterial
- glycerol
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Abstract
The invention belongs to the field of sanitary products and aims to provide a sterilizing hand washing solution product which is efficient and safe and is processed by adopting a modern biotechnological product. The sterilizing hand washing solution product comprises the following components: a surfactant, glycerol, an auxiliary agent and de-ionized water; the hand washing solution contains a sterilizing agent; the sterilizing agent is a bacillus subtilis fermentation solution extractive; the weight ratio of the sterilizing agent is 0.1%-1%. A preparation method of the hand washing solution comprises the following steps: adding the de-ionized water into a reaction kettle and heating the de-ionized water to 65 DEG C; adding the glycerol and the surfactant; agitating uniformly; reducing the temperature to 40 DEG C and adjusting the pH (Potential of Hydrogen) value to 6.5-7.0; adding the sterilizing agent, an essence and the auxiliary agent; agitating, degassing and packaging. According to the hand washing solution provided by the invention, a dirt removing function of the hand washing solution is realized and the hand washing solution also has good sterilization and disinfection functions; meanwhile, the safety is very high; the sterilizing agent added in the hand washing solution has an anticorrosion function and has the characteristics of safety and high efficiency, so that a preservative does not need to be added into the hand washing solution.
Description
Technical field
The invention belongs to field of hygiene products, relate to a kind of special handwashing liquid.
Background technology
Wanting to protect from infection, is at first the article of avoiding contact stain to cross, but in our Working Life, some article can't be avoided contacting.So we must form the custom of often washing one's hands.The articles for washing of selling in the market, be mainly to take decontamination as main, and major part does not have the function of sterilization.
On market, also occurred that some have the handwashing liquid of disinfecting and sterilizing functions.Be mainly to have added some disinfectant, make handwashing liquid have certain bactericidal effect.These disinfectant, some is not bery safe to people, if rinsing is unclean, people's skin is had to certain stimulation.Some disinfectant, expensive, be unfavorable for reducing production costs and increase the competitiveness of product.At present, on market, be starved of a kind of dirt-removing power strong, sterilizing power is strong, safety, cheap handwashing liquid.
Summary of the invention
The purpose of this invention is to provide a kind of highly effective and safe, adopt the disinfection hand cleanser product of modern biotechnology product processing.
Component of the present invention comprises surfactant, glycerol, antibacterial, adjuvant, deionized water.
Technical characteristics of the present invention be added have inexpensive, highly effective and safe, the antibacterial through modern biotechnology processing of All Pure Nature.Antibacterial is bacillus subtilis (Bacillus subtilis) fermentation broth extract, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 16th, 2012, the preservation address is positioned at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3, (postcode 100101), deposit number are CGMCC No.6012.
Fungus characteristic is as follows:
1. somatic cells is Gram-positive living in direct rod shape, spore or that near-end is given birth to.
2. colony characteristics is that white is circular, protuberance, and smooth surface, opaque, moistening, provoke adhesion.
3. can utilize various saccharides, as glucose, galactose, xylose, cellobiose, cottonseed sugar etc.; Produce amylase, protease, can gelatin hydrolysate, V.P, the C.I. 13020. positive.
4. the suitableeest cultivation temperature is 37 ℃, optimum pH 7.0.
This bacterium classification status is as follows: antibacterial territory (Bacteria); Firmicutes (Firmicutes); Bacillus cereus guiding principle (Bacillales); Bacillus cereus order (Bacillaceae); Bacillaceae (Bacillus); Bacillus (Bacillus); Bacillus subtilis strain (Bacillus subtilis).
In the present invention, the preparation method of fermentation broth extract is as follows:
The seed culture medium of bacterial strain is broth bouillon, Carnis Bovis seu Bubali cream 2g wherein, peptone 8g, NaCl5g, water 1000ml, pH value 7.0～7.2, high pressure steam sterilization.
The seed liquor condition of culture: cultivation temperature is 33 ℃, and shaking speed is 140 rev/mins, and fermentation time is 13 hours.
Inoculum concentration is 8%, is conducive to the formation of antibacterial substance.
The fermentation fermentation medium consists of: glucose 0.3%, peptone 1%, NaCl0.5%, MgSO
4.7H
2O0.7%, KH
2PO
40.7%, pH8.0.
Fermentation culture conditions is: cultivation temperature is 33 ℃, and shaking speed is 140 rev/mins, and fermentation time is about 24 hours.
From fermentation liquid, extracting the antibacterial substance method, it is organic solvent precipitation method.
At first by the fermentation liquid high speed centrifugation, temperature 0-4 ℃, rotating speed are 8000 rev/mins, centrifugal ten minutes, retain supernatant.
(methanol: ethanol=1:6), fully dissolving is placed in there-necked flask, and 40 degrees centigrade of temperature are controlled in water-bath, and mixing speed is 60rpm, and the time is 5 hours, collects organic facies, adopts vacuum distillation method to remove organic solvent in supernatant, to add the equal-volume organic solvent.Vapo(u)rizing temperature 30-60 ℃, distilled 20 minutes, and organic solvent is removed.The collecting precipitation material.Obtain antibacterial.
The crude extract of Fungicidal substance, with after water dissolution, concentration is the 1%(mass volume ratio).Lysate can kill common pathogenic bacterium effectively.For example not only can effectively kill escherichia coli, Salmonella, the pathogenic microorganism that golden yellow common coccus etc. are common, and can kill the common coccus of methicillin-resistant staphylococcus.
Method for making of the present invention is simple, deionized water adds in reactor, its temperature is heated to 65 ℃, then add successively glycerol (8～23%), surfactant (18～30%), speed of agitator is unsuitable too high, can be controlled in 20～30 rev/mins, after stirring, cool the temperature to 40 ℃, with citric acid, the pH value of solution is adjusted to 6.5～7.0, antibacterial (0.1～1%), essence is a small amount of, adjuvant (1～5%), stir, speed of agitator can be controlled in 20～30 rev/mins, carry out the feed liquid be stirred degassed, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removal forms in the stirring and dissolving process bubble, reduce the air content of material, mixed liquor is carried out to packing, can obtain handwashing liquid.Ratio accounts for the percentage by weight of product gross weight for each component herein.
Surfactant is sodium lauryl sulphate, Cortex cocois radicis oil base diglycollic amide, fatty alcohol-polyoxyethylene ether, betanin, Sodium Alcohol Ether Sulphate or its mixture.
Adjuvant is polyethylene glycol stearate diester etc.
Essence used can add according to the odor type of product, makes product have different odor types, meets the needs of different consumers.
The invention has the beneficial effects as follows: the present invention has not only realized the function of handwashing liquid removal dirt, and the function of good sterilization and sterilization, particularly can kill the characteristic of methicillin-resistant staphylococcus aureus.The emergency toxicology experiment has also verified the antibacterial added in this detergent people and animals to be had to very high safety.Scientist has found methicillin-resistant staphylococcus aureus in 1961, find that at present it spreads all over the world, and becomes important infectious bacteria, has brought great threat for people's health.The production of the antibacterial added in handwashing liquid, utilize fermentation raw material with low cost, adopts modern biotechnology, and technique is simple, with low cost.Make the present invention, become a kind of sterilizing power strong, safety, cheapness, handwashing liquid with high content of technology.
That in the present invention, adds has the antiseptical function by the pure natural fermentation raw material through the antibacterial that modern biotechnology processes, and has characteristics safely and efficiently.In handwashing liquid without adding again antiseptic.
The specific embodiment
Induction mutation of bacterium
Take the ultraviolet mutagenesis method: the CICC10023 of take is starting strain, makes bacteria suspension, coats on the culture medium plate, in 37 ℃ of incubators, cultivates after ultra-vioket radiation 90s.
Bacterial screening
From the 90s plate, choose 30 single bacterium colonies of looking strong, be inoculated on inclined-plane, in 37 ℃ of incubators, cultivated 0--4 ℃ of stored refrigerated 24 hours; With original strain in contrast, adopt the conventional herd breeding condition, sodium carboxymethyl cellulose is as carbon source and Proteolytic enzyme circle method screening cellulase-producing and the strong bacterial strain of protease, screening obtains cellulase-producing and strong bacterial strain bacillus subtilis (Bacillus subtilis) L1 of protease, this bacterial strain cellulase-producing energy force rate original strain has improved 25%, produces protease energy force rate original strain and has improved 52%.In the center preservation of Chinese common micro-organisms culture presevation administration committee's common micro-organisms, deposit number is CGMCC No6012 by this strain.
This product bacillus subtilis used (Bacillus subtilis) strain, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 16th, 2012, the preservation address is positioned at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3, (postcode 100101), deposit number are CGMCC No6012.
Research shows by experiment: antibacterial have good inhibition escherichia coli, staphylococcus aureus, etc. the characteristics of pathogenic bacterium.
The invention will be further described below in conjunction with specific embodiment, but be not limited to embodiment.
Embodiment 1
Prepare 100 kilograms of products, the 50kg deionized water is added in reactor, its temperature is heated to 65 ℃, then add successively 9kg glycerol, the 9kg sodium lauryl sulphate, the 5kg fatty alcohol-polyoxyethylene ether, the 5kg Sodium Alcohol Ether Sulphate, speed of agitator is unsuitable too high, can be controlled in 20～30 rev/mins, after stirring, cool the temperature to 40 ℃, with citric acid, the pH value of solution is adjusted to 6.5～7.0, 0.2kg antibacterial, 0.0001kg rose essence, the 2kg polyethylene glycol mono stearate, insufficient section deionized water polishing, stir, speed of agitator can be controlled in 20～30 rev/mins, carry out the feed liquid be stirred degassed, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removal forms in the stirring and dissolving process bubble, reduce the air content of material, mixed liquor is carried out to packing, can obtain handwashing liquid.
Product of the present invention adopts conventional microorganism detection bactericidal effect, and result of the test shows, product bactericidal effect of the present invention reaches more than 85%, and bactericidal effect is obvious.Escherichia coli, Salmonella, golden yellow common coccus are selected in microorganism.
Embodiment 2
Prepare 100 kilograms of products, the 60kg deionized water is added in reactor, its temperature is heated to 65 ℃, then add successively 12kg glycerol, the 9kg sodium lauryl sulphate, the 6kg fatty alcohol-polyoxyethylene ether, the 5kg Sodium Alcohol Ether Sulphate, 25 rev/mins of speeds of agitator, after stirring, cool the temperature to 40 ℃, with citric acid, the pH value of solution is adjusted to 6.5～7.0, 0.6kg antibacterial, 0.0003kg rose essence, the 1kg polyethylene glycol mono stearate, insufficient section deionized water polishing, stir, speed of agitator can be controlled in 20～30 rev/mins, carry out the feed liquid be stirred degassed, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removal forms in the stirring and dissolving process bubble, reduce the air content of material, mixed liquor is carried out to packing, can obtain handwashing liquid.
Claims (5)
1. a handwashing liquid, comprise surfactant, glycerol, adjuvant, deionized water, it is characterized in that, contains antibacterial in described handwashing liquid, and described antibacterial is the fermentation broth extract of bacillus subtilis CGMCC6012.
2. handwashing liquid according to claim 1, is characterized in that, described antibacterial part by weight is 0.1～1%.
3. handwashing liquid preparation method according to claim 1, it is characterized in that, comprise the steps: that deionized water adds in reactor, be heated to 65 ℃, add glycerol, surfactant, stir, be cooled to 40 ℃, pH value is adjusted to 6.5～7.0, adds antibacterial, essence and adjuvant, stirs, degassed, packing.
4. handwashing liquid preparation method according to claim 3, is characterized in that, described adjuvant is polyethylene glycol stearate diester.
5. handwashing liquid preparation method according to claim 3, prepare 100 kilograms of products, the 50kg deionized water is added in reactor, its temperature is heated to 65 ℃, then add successively 9kg glycerol, the 9kg sodium lauryl sulphate, the 5kg fatty alcohol-polyoxyethylene ether, the 5kg Sodium Alcohol Ether Sulphate, speed of agitator is unsuitable too high, can be controlled in 20～30 rev/mins, after stirring, cool the temperature to 40 ℃, with citric acid, the pH value of solution is adjusted to 6.5～7.0, 0.2kg antibacterial, 0.0001kg rose essence, the 2kg polyethylene glycol mono stearate, insufficient section deionized water polishing, stir, speed of agitator can be controlled in 20～30 rev/mins, carry out the feed liquid be stirred degassed, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removal forms in the stirring and dissolving process bubble, reduce the air content of material, mixed liquor is carried out to packing, can obtain handwashing liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102864390A CN103405378A (en) | 2012-07-14 | 2013-07-09 | Hand washing solution |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210245408 CN103142455A (en) | 2012-07-14 | 2012-07-14 | Hand sanitizer |
| CN201210245408.6 | 2012-07-14 | ||
| CN2013102864390A CN103405378A (en) | 2012-07-14 | 2013-07-09 | Hand washing solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103405378A true CN103405378A (en) | 2013-11-27 |
Family
ID=48540974
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210245408 Pending CN103142455A (en) | 2012-07-14 | 2012-07-14 | Hand sanitizer |
| CN2013102864390A Pending CN103405378A (en) | 2012-07-14 | 2013-07-09 | Hand washing solution |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210245408 Pending CN103142455A (en) | 2012-07-14 | 2012-07-14 | Hand sanitizer |
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|---|---|
| CN (2) | CN103142455A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450320A (en) * | 2014-12-08 | 2015-03-25 | 成都锦汇科技有限公司 | Rose-flavor liquid soap |
| CN104498232A (en) * | 2014-12-08 | 2015-04-08 | 成都锦汇科技有限公司 | Cool type rose fragrance hand-washing solution |
| CN108498447A (en) * | 2018-03-15 | 2018-09-07 | 广州佳静健康产业有限公司 | A kind of plant enzyme cleaning bubble liquid preparation |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507703A (en) * | 2008-03-12 | 2009-08-19 | 北京绿岛缘蜂产品有限公司 | High-efficiency sterilization hands washing liquid |
-
2012
- 2012-07-14 CN CN 201210245408 patent/CN103142455A/en active Pending
-
2013
- 2013-07-09 CN CN2013102864390A patent/CN103405378A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507703A (en) * | 2008-03-12 | 2009-08-19 | 北京绿岛缘蜂产品有限公司 | High-efficiency sterilization hands washing liquid |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450320A (en) * | 2014-12-08 | 2015-03-25 | 成都锦汇科技有限公司 | Rose-flavor liquid soap |
| CN104498232A (en) * | 2014-12-08 | 2015-04-08 | 成都锦汇科技有限公司 | Cool type rose fragrance hand-washing solution |
| CN108498447A (en) * | 2018-03-15 | 2018-09-07 | 广州佳静健康产业有限公司 | A kind of plant enzyme cleaning bubble liquid preparation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103142455A (en) | 2013-06-12 |
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Application publication date: 20131127 |