CN101507824B - High-efficiency sterilization clothes disinfector - Google Patents
High-efficiency sterilization clothes disinfector Download PDFInfo
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- CN101507824B CN101507824B CN200810101769A CN200810101769A CN101507824B CN 101507824 B CN101507824 B CN 101507824B CN 200810101769 A CN200810101769 A CN 200810101769A CN 200810101769 A CN200810101769 A CN 200810101769A CN 101507824 B CN101507824 B CN 101507824B
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Abstract
The invention discloses an efficient bactericidal clothes disinfectant. The invention aims to provide a bactericidal clothes disinfectant product which is efficient and safe and is processed by adopting modern biotechnology. Main components of the disinfectant are surfactant, bactericide, thickening stabilizer, essence and deionized water. The disinfectant has good functions of sterilizing and disinfecting, and is simple in process and low in cost through the modern biotechnology. The clothes disinfectant has the advantages of strong sterilizing capability, safety, low price and high technical content.
Description
The invention belongs to field of hygiene products, relate to special clothes disinfector.
Background technology:
The clothes disinfector of selling on the market mainly contains the function of removing the medicated clothing dirt.Along with people's is to the more and more attention of health, and the clothes disinfector with disinfection function receives the welcome in market.
At present, the clothes disinfector of selling on the market is normally by several kinds of surfactants, and the adding disinfectant processes.Mostly the disinfectant that adds is many chlorine class, peroxide, and this series products existence is inappropriate for colour clothes, and penetrating odor is arranged, and metal is had characteristics such as corrosion.These disinfectant, some is not really safe to people, if rinsing is unclean, people's skin is had certain stimulation.At present, it is strong to be starved of a kind of sterilizing power on the market, safety, cheap clothes disinfector.
Summary of the invention:
The purpose of this invention is to provide a kind of highly effective and safe, adopt the sterilization clothes disinfector product of modern biotechnology product processing.
Component of the present invention comprises surfactant, antibacterial, thickening stabilizing agent, essence, deionized water.
Major technique characteristic of the present invention be added have inexpensive, highly effective and safe, all-natural is through the antibacterial of modern biotechnology processing.The used antibacterial of the present invention is the inventor's an invention achievement.Antibacterial is bacillus subtilis LD-8 (Bacillus subtilis LD-8) fermentation broth extract; Culture presevation is in China Microbial Culture Preservation Commission common micro-organisms center; Datun Road, Chaoyang District, Beijing City, preservation address; Preservation date December in 2007 18 days, culture presevation numbering CGMCC 2294.
Fungus characteristic is following:
1. somatic cells is life or the living Gram-positive of near-end in direct rod shape, the spore.
2. colony characteristics is that white is circular, protuberance, and smooth surface, opaque, moistening, provoke adhesion.
3. can utilize various saccharides, like glucose, galactose, xylose, cellobiose, cottonseed sugar etc.; Produce amylase, protease, can gelatin hydrolysate, V.P, C.I. 13020. are positive.
4. 37 ℃ of the righttest cultivation temperature, optimum pH 7.0.
This bacterium classification status is following: antibacterial territory (Bacteria); Firmicutes (Firmicutes); Bacillus cereus guiding principle (Bacillales); Bacillus cereus order (Bacillaceae); Bacillaceae (Bacillus); Bacillus (Bacillus); Bacillus subtilis strain (Bacillus subtilis).
The method for preparing of fermentation broth extract is following among the present invention:
The seed culture medium of bacterial strain is a broth bouillon, Carnis Bovis seu Bubali cream 3g wherein, peptone 10g, NaCl 5g, water 1000ml, pH value 7.0-7.2, high pressure steam sterilization.
The seed liquor condition of culture: cultivation temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is 10～14 hours.
Inoculum concentration is 6%～10% o'clock, helps the formation of antibacterial substance.
The fermentation fermentation medium of optimizing consists of: sucrose 0.3%, and peptone 1%, NaCl 0.5%, MgSO
4.7H
2O0.7%, KH
2PO
40.7%, pH8.0.
The fermentation culture conditions of optimizing is: cultivation temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is about 24 hours.
From fermentation liquid, extracting the antibacterial substance method is organic solvent precipitation method.Available organic solvent is acetone, methanol, ethanol.
At first with fermentation liquid, add high speed centrifuge, temperature 0-4 ℃, rotating speed is 10000 rev/mins, centrifugal ten minutes, remove deposition, and keep supernatant.
With the supernatant that obtains, add isopyknic methanol, the temperature of system is reduced to-5 ℃ rapidly, preserved 2 hours for-5 ℃.Collect organic facies, adopt vacuum distillation method to remove organic solvent.Vapo(u)rizing temperature 30-60 ℃, distilled 20 minutes, organic solvent is removed.The collecting precipitation material.This promptly is the Fungicidal substance that we obtain.
The crude extract of Fungicidal substance, behind water dissolution, concentration is 1% (mass volume ratio).Lysate can kill common pathogenic bacterium effectively.For example not only can kill escherichia coli effectively, Salmonella, the pathogenic microorganism that golden yellow common coccus etc. are common, and can kill the common coccus of methicillin-resistant staphylococcus.
This bactericidal liquid has high safety; In the emergency toxicology experiment, we irritate 5 groups of 200 mouse of clothes with this bactericidal liquid, and the mouse of experimental group none occur dead; And bacillus subtilis is present in occurring in nature widely, and many important industrial bacterial strains are exactly bacillus subtilis.Basically can conclude that this bactericidal liquid is safe for user, can relievedly use.After adding it to clothes disinfector, find that it still has good bactericidal activity.
Method for making of the present invention is simple, adds at first deionized water (65～85%) to be added in the agitated reactor man-hour, and its temperature is heated to 70 ℃, adds surfactant (10～15%) then successively; Thickening stabilizing agent (3～10%) after stirring, cools the temperature to 40 ℃; With citric acid the pH value of solution is transferred to 6.5～7.0, add antibacterial (2～10%), add essence; Stir, speed of agitator can be controlled in 20～30 rev/mins, and the feed liquid that stirs is outgased; Adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removes the bubble that in the stirring and dissolving process, forms; Reduce the air content of material, mixed liquor is carried out packing, can obtain high-efficiency sterilization clothes disinfector.Ratio is the percentage by weight of each ingredients constitute product gross weight here.
Surfactant is quaternary cationic surfactants such as tetra-alkyl ammonium chloride.
Thickening stabilizing agent is one or more combination such as xanthan gum, carrageenan, sodium alginate.
Used essence can add according to the odor type of product, makes product have different odor types, satisfies the needs of different consumers.
Beneficial effect:
The present invention has possessed good antibacterial and disinfectant function, particularly can kill the characteristic of methicillin-resistant staphylococcus aureus.The emergency toxicology experiment has also verified the antibacterial that adds in this clothes disinfector people and animals to be had very high safety.Scientist has found methicillin-resistant staphylococcus aureus in 1961, find that at present it spreads all over the world, and becomes important infectious bacteria, has brought great threat for people's health.The production of the antibacterial that adds in the clothes disinfector utilizes fermentation raw material with low cost, adopts modern biotechnology, and technology is simple, and is with low cost.Make the present invention, it is strong to become a kind of sterilizing power, safety, cheapness, clothes disinfector with high content of technology.
That adds among the present invention has the antiseptical function by the pure natural fermentation raw material through the antibacterial that modern biotechnology processes, and has characteristics safely and efficiently.Need not to add again antiseptic in the liquid detergent.
Specific embodiment:
Below in conjunction with specific embodiment the present invention is described further, but is not limited to embodiment.
Embodiment 1
Prepare 100 kilograms of products, the 50kg deionized water is added in the agitated reactor, its temperature is heated to 70 ℃, add the 10kg tetra-alkyl ammonium chloride then successively; The 5kg xanthan gum after stirring, cools the temperature to 40 ℃, with citric acid the pH value of solution is transferred to 6.5; Add the 5kg antibacterial, add 0.0001% rose essence, deionized water polishing weight stirs, and speed of agitator can be controlled in 25 rev/mins; The feed liquid that stirs is outgased, adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removes the bubble that in the stirring and dissolving process, forms; Reduce the air content of material, mixed liquor is carried out packing, can obtain high-efficiency sterilization clothes disinfector.
100 kilograms of products of embodiment 2 preparations,
The 75kg deionized water is added in the agitated reactor, its temperature is heated to 70 ℃, add the 15kg tetra-alkyl ammonium chloride then successively, 5kg xanthan carrageenan; After stirring, cool the temperature to 40 ℃, the pH value of solution is transferred to 7.0, add the 5kg antibacterial with citric acid; Add the 0.0001kg rose essence, stir, speed of agitator can be controlled in 20～30 rev/mins, and the feed liquid that stirs is outgased; Adopt vacuum outgas, negative pressure can be controlled in 5Mpa, removes the bubble that in the stirring and dissolving process, forms; Reduce the air content of material, mixed liquor is carried out packing, can obtain high-efficiency sterilization clothes disinfector.
Get 9% concentration product of the present invention, adopt conventional microorganism detection bactericidal effect, result of the test shows that product bactericidal effect of the present invention reaches more than 90%, and bactericidal effect is obvious.Escherichia coli, Salmonella, golden yellow common coccus are selected in microorganism.
Claims (5)
1. a clothes disinfector comprises surfactant, antibacterial, thickening stabilizing agent, essence and deionized water, it is characterized in that,
Said surfactant is quaternary cationic surfactants such as tetra-alkyl ammonium chloride;
Said antibacterial is bacillus subtilis LD-8 (Bacillus subtilis LD-8) CGMCC2294 fermentation broth extract, and culture presevation is in China Microbial Culture Preservation Commission common micro-organisms center, and the fermentation broth extract method for preparing is:
The seed culture medium of bacterial strain is a broth bouillon, Carnis Bovis seu Bubali cream 3g wherein, peptone 10g, NaCl 5g, water 1000ml, pH value 7.0 ~ 7.2, high pressure steam sterilization;
The seed liquor condition of culture: cultivation temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is 10 ~ 14 hours;
The fermentation medium of optimizing consists of: sucrose 0.3%, and peptone 1%, NaCl 0.5%, and MgSO4.7H2O 0.7%, and KH2PO4 0.7%, pH8.0;
The fermentation culture inoculum concentration is 6% ~ 10%;
The fermentation culture conditions of optimizing is: cultivation temperature is 37 ℃, and shaking speed is 140 rev/mins, and fermentation time is about 24 hours;
From fermentation liquid, extract antibacterial substance through organic solvent precipitation method, available organic solvent is acetone, methanol, ethanol;
With fermentation liquid, add high speed centrifuge, temperature 0-4 ℃, rotating speed is 10000 rev/mins, centrifugal ten minutes, remove deposition, keep supernatant;
With the supernatant that obtains, add isopyknic methanol, the temperature of system is reduced to-5 ℃ rapidly, preserved 2 hours for-5 ℃; Collect organic facies, adopt vacuum distillation method to remove organic solvent, vapo(u)rizing temperature 30-60 ℃; Distilled 20 minutes, and organic solvent was removed the collecting precipitation material;
Said thickening stabilizing agent is one or more combination such as xanthan gum, carrageenan, sodium alginate.
2. according to the said clothes disinfector of claim 1, the percentage by weight that it is characterized in that said antibacterial is 0.1-1%.
3. according to the said clothes disinfector method for preparing of claim 1, its preparation method is following: deionized water adds in the agitated reactor, is heated to 70 ℃, adds the surfactant that percentage by weight is respectively 10-15%; The thickening stabilizing agent of 3-10% stirs, and is cooled to 40 ℃; PH value transfers to 6.5-7.0, adds the 2-10% antibacterial, essence; Stir the degassing, packing ratio.
4. according to the said clothes disinfector method for preparing of claim 3, it is characterized in that surfactant is a tetra-alkyl ammonium chloride.
5. according to the said clothes disinfector method for preparing of claim 3, it is characterized in that stabilizing agent is at least a in xanthan gum, carrageenan, the sodium alginate.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810101769A CN101507824B (en) | 2008-03-12 | 2008-03-12 | High-efficiency sterilization clothes disinfector |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810101769A CN101507824B (en) | 2008-03-12 | 2008-03-12 | High-efficiency sterilization clothes disinfector |
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| CN101507824A CN101507824A (en) | 2009-08-19 |
| CN101507824B true CN101507824B (en) | 2012-09-05 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994256B (en) * | 2016-05-27 | 2018-12-07 | 贵州大学 | Film and preparation method thereof is inhaled in the HZSND resistance of prevention and control rice hoppers |
| CN106075500B (en) * | 2016-06-23 | 2019-07-05 | 刘敏 | A kind of fungicide and preparation method thereof for β-Ultrasound Room instrument |
| CN108902144A (en) * | 2018-07-05 | 2018-11-30 | 安徽羽洁家纺有限公司 | A kind of dedicated bactericidal agent of natural feather |
| CN117918351A (en) * | 2024-01-18 | 2024-04-26 | 广东星帮尼科技股份有限公司 | Quaternary ammonium salt disinfectant, preparation method and application thereof in clothes cleaning |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1432284A (en) * | 2002-01-18 | 2003-07-30 | 福建泰禾生化科技股份有限公司 | High-efficiency biological germicide and its prepn process |
| CN1766091A (en) * | 2005-09-23 | 2006-05-03 | 中国农业大学 | Bacillus subtilis and its uses |
| CN101041846A (en) * | 2007-01-29 | 2007-09-26 | 中国科学院等离子体物理研究所 | Preparation method and usage for novel lipopeptide type biosurfactant surfactin |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1432284A (en) * | 2002-01-18 | 2003-07-30 | 福建泰禾生化科技股份有限公司 | High-efficiency biological germicide and its prepn process |
| CN1766091A (en) * | 2005-09-23 | 2006-05-03 | 中国农业大学 | Bacillus subtilis and its uses |
| CN101041846A (en) * | 2007-01-29 | 2007-09-26 | 中国科学院等离子体物理研究所 | Preparation method and usage for novel lipopeptide type biosurfactant surfactin |
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