KR102386108B1 - Abrasive composition for pet snack and pet snack composition comprising the same - Google Patents

Abstract

The present invention relates to a method for producing an abrasive composition for companion animal snacks, an abrasive composition for companion animal snacks prepared according to the method, and a companion animal snack composition comprising the same. The method includes the steps of: (a) heat-treating a pear pulverized solution, filtering the same to remove pear juice, and drying a by-product to obtain pear dreg powder; (b) pulverizing the pear dreg powder and then passing the same through a 30 mesh net to screen the same to a size that does not pass through a 60 mesh net so that sorted pear dreg powder is obtained; (c) reacting the sorted pear dreg powder with alcohol to obtain a precipitate; and (d) enzymatically treating the precipitate with Aspergillus niger-derived Rapidase, removing a supernatant from an enzymatically treated product, and drying the precipitate with hot air. Accordingly, compared to conventional companion animal snack compositions such as commercial cellulose dog gums or dog gums containing non-enzyme-treated pear dreg components, the present invention has remarkably excellent effects such as proper polishing of companion animal teeth, prevention and removal of plaque and tartar, and suppression of gingivitis. Also, natural ingredients are used, and thus the composition of the present invention is harmless when ingested.

Description

Abrasive composition for companion animal snack comprising pear pear enzyme-treated product, and companion animal snack composition comprising same

The present invention relates to an abrasive composition for a companion animal snack comprising an enzyme-treated pear pear, and a companion animal snack composition comprising the same, and more particularly, to an abrasive composition for a companion animal snack comprising an enzyme-treated pear basil-containing product And it relates to a companion animal snack composition comprising the same.

Stone cells, also called reinforcing cells or sclerenchymal cells, are shorter than fibrous cells and have a thick, irregular and very hard secondary cell wall. Stone cells are found in the tissues of fruits, especially in the flesh of the genus Pyrus. Stone cells are a type of lignification in which lignin is deposited on the cell wall within the tissue, and it is known that it serves as a frame for fixing the cell wall shape so that it does not change by strengthening the cell wall firmly.

The main component of stone cells in pear fruit was studied by Ranadive and Haard in Western pear a long time ago. It is known that 18 to 35% of lignin not found in the primary cell wall is contained. Also, the main units of lignin are vanillin and syringaldehyde.

On the other hand, dog gum, which is a representative snack for companion animals, is mainly manufactured from natural cowhide linings of animals, and is made as a toy and snack that can be chewed or eaten by companion animals such as dogs. In addition, as it has a tartar removal function, it can help dental health and relieve stress in companion animals. Such dog gum has been developed using various ingredients and various methods to manufacture such dog gum, out of the basic form of using cowskin as a material in the prior art.

In particular, companion animals have difficulty brushing their teeth frequently, so if you neglect this, gingivitis, plaque, and tartar may occur. Therefore, even if you do not brush your teeth separately, you can suppress the occurrence of gingivitis, plaque, and tartar just by consuming snacks such as dog gum on a regular basis. The development of companion animal snacks is required.

Korean Patent Publication No. 10-2020-0124533 Korean Patent Publication No. 10-2019-0006238

An object of the present invention is that effects such as proper grinding of animal teeth, prevention and removal of plaque and tartar, and suppression of gingivitis are significantly superior to conventional companion animal snack compositions or companion animal snack compositions comprising non-enzyme-treated pear pear components. , It is to provide an abrasive composition for companion animal snacks containing an enzyme-treated product of pear pear which is harmless even if the companion animal ingests the component of the companion animal snack composition when ingested by using natural materials as an abrasive component, and a companion animal snack composition comprising the same .

Another object of the present invention is to prepare the abrasive composition for companion animal snacks using pear pear without using a complicated process or harmful chemicals, selecting the pear pear material by particle size, and performing alcohol treatment and enzyme treatment for companion animal teeth Abrasive composition for companion animal snacks containing enzyme-treated pear pear which has significantly superior abrasive effect, gingivitis, plaque and tartar inhibitory effect compared to conventional commercial companion animal snack compositions or companion animal snack compositions containing non-enzyme-treated pear pear components An object of the present invention is to provide a method for preparing a method for manufacturing and a method for preparing a companion animal snack composition comprising the same.

According to one aspect of the present invention,

(a) heat-treating the crushed pear liquid, then filtering to remove the pear juice, and drying the by-product to obtain a pear pear powder;

(b) pulverizing the pear pear powder, and then selecting a size that passes through a 30 mesh mesh and does not pass through a 60 mesh mesh to obtain a screened pear pear powder;

(c) reacting the selected pear basil powder with alcohol to obtain a precipitate; and

(d) enzymatically treating the precipitate with Aspergillus niger-derived Rapidase, removing the supernatant from the enzyme-treated product, and drying the precipitate with hot air; A method of making the composition is provided.

In step (a), the heat treatment may be performed at 100 to 140 ℃.

In step (c), the alcohol may be a lower alcohol having 1 to 4 carbon atoms.

The alcohol may be an aqueous solution of ethanol having a concentration of 40 to 50%.

In step (d), the Aspergillus niger-derived rapidase (Rapidase) may be added in an amount of 0.01 to 0.15 wt% of the solid matter.

In step (d), the Aspergillus niger-derived rapid Dase (Rapidase) is cellulase (cellulase), hemicellulase (hemicellulase), beta glucanase (β-glucanse) and pectinase ( pectinase) may be a complex enzyme.

In step (d), the enzymatic treatment may be performed at 35 to 40° C. for 8 to 20 hours.

In step (d), the hot air drying may be performed at 50 to 70 ℃ for 10 to 20 hours.

According to another aspect of the present invention,

An abrasive composition for dog gum prepared according to the above manufacturing method is provided.

The abrasive composition for dog gum may have a total dietary fiber content of 75 to 85% (w/v).

The abrasive composition for dog gum may have an insoluble dietary fiber content of 65 to 80% (w/v).

The stone cell content may be 85 to 95% based on the total weight of the insoluble dietary fiber.

According to another aspect of the present invention, there is provided a dog gum composition comprising the abrasive composition for dog gum prepared according to the manufacturing method.

The dog gum composition may include 5 to 20 wt% of the abrasive composition for dog gum based on the total weight.

The dog gum composition may have an acidity of 0.90 to 0.96% based on citric acid.

The dog gum composition may have a hardness of 11000 to 15000 g/cm ² .

The dog gum composition may be for removing or inhibiting gingivitis, plaque or tartar.

The abrasive composition for a companion animal snack comprising the enzyme-treated pear containing stone cells of the present invention and the companion animal snack composition comprising the same are suitable for proper polishing of companion animal teeth, prevention and removal of plaque and tartar, suppression of gingivitis and The same effect is significantly superior to conventional commercial companion animal snack compositions or companion animal snack compositions containing non-enzyme-treated pear pear ingredients. there is.

Another method of the present invention for preparing an abrasive composition for companion animal snack comprising an enzyme-treated product of pear containing stone cells and a method for preparing a companion animal snack composition comprising the same is a complex composition of an abrasive composition for companion animal snack using pear A conventional commercial companion animal snack composition that does not use any process or harmful chemicals, selects pear materials by particle size, and performs alcohol treatment and enzyme treatment, and has the effect of suppressing tooth grinding, gingivitis, plaque and tartar in companion animals. However, it is significantly superior to the companion animal snack composition containing pear pear components that have not been treated with enzymes.

1 is a photograph of a dog gum composition according to Examples 1 to 3 and Comparative Example 1. FIG.
Figure 2 is a stereoscopic microscope photograph of the pear powder sample according to Experimental Example 1 after staining.
3 is a standard curve according to a color difference value and a photograph stained according to a stone cell concentration for examining stone cell purity in Experimental Example 1. FIG.
4 is a photograph of the fecal evaluation criteria according to Experimental Example 3.
5 is a gingival index (GI), plaque index (PI), and calculus index (CI) measurement results of an untreated control group that did not brush according to Experimental Example 3.
6 is a result of measuring the gingival index (GI), plaque index (PI), and calculus index (CI) in the teeth of the experimental beagle according to Experimental Example 3.
7 is a photograph showing the state of teeth of the beagle after 4 weeks of ingestion of the dog gum composition according to Experimental Example 3.
8 is a fecal evaluation result according to Experimental Example 3.

Since the present invention can apply various transformations and can have various embodiments, specific embodiments are illustrated in the drawings and described in detail in the detailed description. However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all modifications, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the present invention, if it is determined that a detailed description of a related known technology may obscure the gist of the present invention, the detailed description thereof will be omitted.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. The singular expression includes the plural expression unless the context clearly dictates otherwise. In the present application, terms such as “comprise” or “have” are intended to designate that a feature, number, step, operation, component, part, or combination thereof described in the specification exists, but one or more other features It should be understood that this does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof.

Hereinafter, a method for manufacturing an abrasive composition for companion animal snacks comprising the enzyme-treated pear pear of the present invention will be described.

First, the crushed pear liquid is heat-treated, filtered to remove the pear juice, and the by-product is dried to obtain a pear pear powder (step a).

The heat treatment is preferably performed at 100 to 140 °C, more preferably 110 to 130 °C, even more preferably 115 to 125 °C.

The filtration is preferably performed 2 to 3 times using a nonwoven fabric to remove the pear juice.

Thereafter, the pear pear powder is pulverized and passed through a 30 mesh mesh and screened to a size that does not pass through a 60 mesh mesh to obtain a screened pear pear powder (step b).

The screened pear powder is preferably selected to a size that passes through a 30 mesh network and does not pass through a 60 mesh network, more preferably a size that passes through a 30 mesh network and does not pass through a 50 mesh network, even more preferably Preferably, it can be used by selecting a size that passes through a 30 mesh network and does not pass through a 50 mesh network. When the size of such particles is selected, the grinding performance can be improved by increasing the density of stone cells.

If the size of the pear component is less than 30 mesh, the particle size of the pear component is large, and the surface of companion animal snacks such as dog gum is rough, which can damage the teeth or gums of the companion animal. can be lowered

In the present invention, the mesh unit was based on KS A 5101 (unit indicating the particle size by a standard sieve), and the mesh and micrometer (㎛) unit conversion table according to this is shown in Table 1 below.

Next, the selected pear basil powder is reacted with alcohol to obtain a precipitate (step c).

In step (c), the alcohol is preferably a lower alcohol having 1 to 4 carbon atoms, more preferably an aqueous ethanol solution having a concentration of 40 to 50%, and even more preferably 43 to 47% of the concentration. It may be an aqueous ethanol solution.

The reaction with the alcohol is preferably reacted with stirring at a speed of 100 to 140 rpm at 20 to 30 °C, and more preferably at 23 to 27 °C at a rate of 110 to 130 rpm while stirring to promote the reaction.

Finally, the precipitate is enzymatically treated with Aspergillus niger-derived rapidase (Rapidase), the supernatant is removed from the enzyme-treated product, and the precipitate is dried with hot air (step d).

The Aspergillus niger (Aspergillus niger)-derived rapidase (Rapidase) is cellulase (cellulase), hemicellulase (hemicellulase), beta glucanase (β-glucanse) and a complex comprising a pectinase (pectinase) is an enzyme

The Aspergillus niger-derived rapidase (Rapidase) is preferably added in an amount of 0.01 to 0.15 wt% of the solid, more preferably 0.05 to 0.13 wt%, even more preferably 0.08 to 0.12 It can be added in wt% content.

As the buffer solution, sodium acetate, sodium phosphate, 2-ethanesulfonic acid (PIPES), water, etc. may be used, and sodium acetate may be preferably used.

The enzyme treatment is preferably performed at 35 to 40 ℃ for 10 to 20 hours. In this case, the abrasive effect, gingivitis, plaque and tartar inhibitory effect can be increased when the abrasive composition for companion animal snack, which has the best enzyme activity and is an enzyme-treated product, is included in the companion animal snack composition.

The obtained enzyme-treated product is centrifuged to obtain a precipitate, which can be used as an abrasive composition for companion animal snacks.

The hot air drying is preferably performed at 50 to 70° C. for 10 to 20 hours, and more preferably at 55 to 65° C. for 13 to 18 hours.

The present invention provides an abrasive composition for a companion animal snack prepared according to the method for preparing the abrasive composition for a companion animal snack.

The abrasive composition for companion animal snacks may have a total dietary fiber content of 75 to 85% (w/v). Here, the total dietary fiber means the content including insoluble dietary fiber and soluble dietary fiber.

In addition, the abrasive composition for companion animal snacks may have an insoluble dietary fiber content of 65 to 80% (w/v), preferably 70 to 75% (w/v). Here, the soluble dietary fiber content is obtained by subtracting the insoluble dietary fiber content from the total dietary fiber content.

In addition, the content of stone cells in the total amount of insoluble dietary fiber may be 85 to 95%, preferably 90 to 93%. Here, stone cell may be defined as a component containing lignocellulose and ligne. When the stone cell content is within the above range, the polishing performance can be significantly improved when used as an abrasive composition for a companion animal snack composition.

The high content of stone cells in the total amount of insoluble dietary fiber in the abrasive composition for companion animal snacks of the present invention is because cellulose, hemicellulose, etc. other than stone cells are removed by the enzyme treatment.

The abrasive composition for companion animal snacks may have a water absorption capacity of 1.2 to 2 ml/g, preferably 1.4 to 1.6 ml/g, based on the lapse of 1 to 24 hours. This low water absorption power seems to be due to the high purity of the stone cells, and has the effect of preventing a decrease in formulation stability due to water absorption when preparing a companion animal snack composition.

The present invention provides a companion animal snack composition comprising the abrasive composition for a companion animal snack.

In the companion animal snack composition of the present invention, various materials that can be used as fillers for companion animal snacks, such as dog gum, may be compounded. For example, powder of cow skin, pork skin, and poultry powder may be used, and rice flour, wheat flour, milk powder, vegetable powder, etc. may be used.

In addition, when ingested, flavoring agents, sweeteners, food coloring, etc. can be added to increase the palatability of companion animals, preservatives can be added for storage, and vitamins or minerals can be added in consideration of nutrition. may be

The companion animal snack composition preferably contains 5 to 20 wt% of the abrasive composition for companion animal snack based on the total weight, and more preferably 7 to 15 wt%. If it is contained in less than 5 wt%, the polishing effect, gingivitis, plaque and tartar inhibitory effect may be reduced, and if it exceeds 20 wt%, it may cause a wound on the tooth surface or may cause irritation or injury to the gums.

The companion animal snack composition may have an acidity of 0.90 to 0.96%, preferably 0.91 to 0.93%, based on citric acid.

In addition, the companion animal snack composition may have a hardness of 11000 to 15000 g/cm ² , and more preferably 11500 to 14500 g/cm ² .

In the above acidity and hardness range, the companion animal snack composition can be stored for a long time, shape deformation can be minimized, and the companion animal's preference can be improved.

The companion animal snack composition may be used for removing or inhibiting gingivitis, plaque or tartar.

The content of fillers and abrasives included in the companion animal snack composition can be adjusted according to the age, size, species, and use of companion animals such as dogs and cats.

The present invention provides a method for preparing a companion animal snack composition comprising the method for preparing an abrasive composition for companion animal snack.

In the present invention, companion animal snacks include dog gum, sweets, feed, jelly, etc. for companion animals such as dogs and cats, and may include various foods and snacks that can manage the teeth of companion animals, including abrasives.

Hereinafter, examples of the present invention, that is, representative dog gum among companion animal snacks will be described, but the scope of the present invention is not limited thereto.

[Example]

Preparation Example 1: Preparation of pear powder

100 g of the ground pear liquid was heated at 120 ° C. for 3 hours under high pressure and filtered twice through a nonwoven fabric to prepare pear juice, and the remaining by-product, pear pear powder, was obtained, and the lightness was measured to be 60.2 - 65.4.

Preparation Example 2: Selected pear powder

After pulverizing the pear pear powder prepared in Preparation Example 1 with a mixer, only 30 to 60 mesh sizes in which stone cells are concentrated were selected to prepare a selective pear basil powder.

Preparation Example 3: Enzyme-treated abrasive composition for dog gum

The selected pear powder prepared according to Preparation Example 2 was treated with ethanol and enzyme treatment.

Specifically, in the ethanol treatment, 10 g of the sample was reacted in a 45% ethanol aqueous solution contained in a 1 liter Erlenmeyer flask at 25° C. at a speed of 120 rpm for 1 hour to dissolve stone cells and dietary fiber. Then, centrifugation was performed at 8,000 rpm for 10 minutes, and the supernatant was removed at 4°C. After washing twice with water, the remaining ethanol component was removed by centrifugation.

Next, the ethanol-treated screening pear powder was enzymatically treated. For the enzyme treatment, the Aspergillus niger-derived rapidase (Rapidase) was added to 0.1% by weight of the solid matter of cellulase, sodium acetate buffer (sodium acetate buffer) (0.01M, pH 5.0) ) was added, and the reaction was performed at 37 ° C. at 80 rpm for 15 hours, centrifuged, and then the supernatant was completely removed and dried at 60 ° C. for 15 hours.

Example 1: Preparation of dog gum composition comprising pear basil powder

A dog gum composition was prepared with the composition shown in Table 2 below, and 10 wt% of the pear baek powder of Preparation Example 1 was included as an abrasive component.

Example 2: Preparation of dog gum composition comprising selected pear basil powder

A dog gum composition was prepared under the same conditions as in Example 1, except that the selected pear baek powder of Preparation Example 2 was included as an abrasive component instead of the pear baek powder of Preparation Example 1.

Example 3: Preparation of dog gum composition comprising enzyme-treated abrasive for tartar removal

A dog gum composition was prepared under the same conditions as in Example 1, except that the enzyme-treated abrasive composition for tartar removal of Preparation Example 3 was included instead of the baebaek powder of Preparation Example 1.

Comparative Example 1: Commercial Cellulose Dog Gum

A composition containing cellulose gum was prepared as an abrasive composition for dog gum.

Ingredients Content (wt%) rice flour 60-70 Abrasives (cellulose gum or Preparation Examples 1-3) 10 Hydrolyzed Chicken 4-7 Hexametaphosphate 0.8~1.2 Potassium Sorbate 0.09~0.13 green tea extract 0.004~0.009 glycerin 9-11 propylene glycol 1-2 sorbitol 3-5 vitamin mixture dose mineral mixture 0.004~0.01 amino acid mixture 0.004~0.01 entire 100

Fig. 1 shows photographs of dog gum compositions prepared according to Examples 2, 3 and Comparative Example 1.

[Experimental example]

Experimental Example 1: Stone cell purity analysis

Dietary fiber was analyzed according to the method of Prosky et al. (1988). For the content of insoluble dietary fiber (IDF), 1 g of the sample was turbid in 50 ml of phosphate buffer (pH 6.0), heat-resistant α-amylase at 95° C., and protease at 60° C. and amyloglucosidase were sequentially enzymatically treated. After the enzyme-treated sample was filtered on the weighed diatomaceous earth, the filtered residue was washed with 75% ethanol, 95% ethanol and acetone, dried for 12 hours, and measured as a value excluding protein and ash content. In addition, for soluble dietary fiber (SDF), 95% ethanol at 60 ° C. was added to the filtrate filtered through diatomaceous earth at a ratio of 1:4, reacted for 1 hour, and then filtered again to measure the dry weight of the remaining residue. .

Total dietary fiber (TDF) content was calculated as the sum of insoluble and soluble dietary fiber content of the residue.

Stone cells can be defined as containing lignocellulose and lignin, and a staining method that can be distinguished from insoluble dietary fiber composed of simple cellulose or hemicellulose is required. Kwon et al. (2016). According to the method, Wiesner staining solution was composed of 2% phloroglucinol, 20% ethanol, and 20% hydrochloric acid (HCl), and was left for about 2 hours after preparation.

2% Phloroglucinol-HCl dyeing solution was added to the pear berry powder of Preparation Example 1, the screened pear basil powder of Preparation Example 2, and the enzyme-treated abrasive powder sample of Preparation Example 3, dyed at room temperature for 1 hour at 100rpm, and then 2.5 times under a stereoscopic microscope. The results of the enlarged observation are shown in FIG. 2 . According to this, stone cells (lignocellulose + lignin) have a red color, and other insoluble dietary fibers such as cellulose and hemicellulose are brown, so stone cells can be distinguished.

Meanwhile, for the quantification of stone cells, a-value was measured using a colorimeter, and the content of stone cells contained in the insoluble dietary fiber powder was quantified using a standard curve to which the concentration and redness value of stone cells were applied. .

For reference, the photo stained according to the concentration of stone cells and the standard curve to which the redness value is applied are shown in FIG. 3 , and the color difference values for each component content are shown in Table 3 below. Here, the stone cell concentration can be measured according to the value of a.

Concentration (mg/mL) L a b c 0 2.47±0.04 0.08±0.03 -1.09±0.08 1.09±0.09 One 4.66±0.02 2.95±0.06 -1.49±0.06 3.31±0.08 3 5.35±0.06 3.92±0.06 -1.65±0.05 4.25±0.07 5 8.03±0.05 7.97±0.09 -1.15±0.04 8.05±0.09 10 6.91±0.07 10.79±0.20 -0.93±0.09 10.83±0.21 20 7.02±0.16 16.23±0.01 -0.77±0.01 16.25±0.40 30 7.57±0.06 17.93±0.05 0.50±0.05 17.93±0.31 40 6.69±0.06 18.69±0.02 0.39±0.02 18.69±0.19 50 7.49±0.06 19.36±0.05 0.59±0.05 19.37±0.27 70 7.31±0.06 19.39±0.05 0.25±0.05 19.39±0.11 100 8.51±0.02 20.00±0.02 0.73±0.02 20.01±0.01

Accordingly, the measured results are shown in Table 4 below.

Kinds total dietary fiber
(wt%) Insoluble fiber content
(%) Stone cell content in insoluble dietary fiber
(%) Water absorption (ml/g) 1 h 3h 24h Preparation Example 1 64.38b 16.21c 25.20c 16.55a 17.93a 19.18a Preparation 2 69.74b 46.41b 66.67b 2.91bc 2.80bc 3.26b Preparation 3 78.08a 72.21a 92.26a 1.36e 1.430d 1.54d

According to this, it was found that the enzyme-treated abrasive for tartar removal of Preparation Example 3 had a high purity of 92% stone cells, and the pear berry powder of Preparation Example 1 had a relatively low stone cell content and 75% of dietary fiber. Moisture absorption power causing formulation instability during dog gum manufacturing was calculated according to Equation 1 below.

[Equation 1]

Water absorption capacity (SC)(ml/g)= (V _wet -V _dry )/W _dry

Here, V _wet is the volume of the hydrated sample, V _dry is the volume of the dry sample, and W _wet is the mass of the dry sample.

According to this, the pear baek powder of Preparation Example 1 having a relatively high soluble dietary fiber ratio and low stone cell content had a very high water absorption at 19 ml/g, and the selected pear baek powder of Preparation Example 2 was 3.2 ml/g, Preparation Example The enzyme-treated abrasive for tartar removal of No. 3 showed very low water absorption even after 24 hours at 1.5ml/g. As can be seen from the microscopic image, it seems to be due to the high purity of the red stone cells. When water absorption is low, it may have high formulation stability.

Experimental Example 2: Quality analysis of dog gum composition

For the dog gum compositions of Examples 2, 3, and Comparative Example 1, quality characteristics of length, thickness, mass, acidity, pH, and hardness were measured.

Specifically, after measuring the weight, length, and thickness of 10 dog gums in each experimental group, the average value was shown. For pH and acidity, 3 g of dog gum sample and 30 ml of distilled water were placed in 50 ml of falcon, heated in a water bath at 8° C. for 10 minutes, and then pulverized with a mixer. The pH was measured at ambient temperature using a pH meter with a glass electrode calibrated with pH 4.00 and 7.00 buffer, and the acidity was measured by adding 2 drops of phenolphthalein to the sample and NaOH consumed at the time when 0.1N NaOH was added to show red color. The amount (ml) was converted to citric acid and expressed as a percentage. Colorimeter (Hunter color, Spectrophotometer NS-800 RT Co., Ltd., SQC8 program) was used to measure the lightness (lightness, L* value), redness (a* value), yellowness (yellowness, b* value) and ΔE (color difference) were measured repeatedly three times, and then expressed as an average value. At this time, the L*, a*, and b* values of the standard white plate used for color correction were 86.31, -0.45 and 0.79, respectively. Hardness was measured using a texturemeter (500Nzwicki, MTM corperation, Germany). For the probe used at this time, hardness was measured under the conditions of start position speed 100 mm/min and pre-load speed 2 mm/s using a 6 mm diameter cylindrical probe (SMS P/6), and repeated experiments a total of 5 times. did

The quality characteristics analysis results for the dog gum compositions of Examples 2, 3, and Comparative Example 1 measured in this way are shown in Table 5 below, and the chromaticity analysis results are shown in Table 6 below.

division Length
(mm) thickness
(mm) weight
(g/piece) acidity
(%) pH Hardness
(g/cm ² ) Comparative Example 1 69.07a 18.23b 18.53a 0.77b 5.63b 11933b Example 2 66.63a 18.77b 18.95a 0.94a 5.84a 13900a Example 3 65.57b 20.7a 20.81a 0.92a 5.90a 11833b

division L* a* b* ΔE* Comparative Example 1 49.54c 4.39c 18.45b 50.45a Example 2 63.64a 7.07b 21.27ab 39.33c Example 3 55.12b 8.22a 21.99a 47.23b

According to this, the dog gum compositions of Examples 2 and 3 except for the cellulose dog gum of Comparative Example 1 had high acidity at 0.94% and 0.92%, respectively, and the hardness of the dog gum composition of Example 2 was 13900 g/cm ² , which was the hardest. and the dog gum compositions of Example 3 and Comparative Example 1 were similar. In the chromaticity of the sample, the brightness L value was 49.54 for the cellulose dog gum of Comparative Example 1, 63.64 for the dog gum of Example 2, and 55.12 for the dog gum of Example 3, and the redness a value was the cellulose dog gum of Comparative Example 1. 4.39, the dog gum of Example 2 was measured as 7.07, and the dog gum of Example 3 was measured as 8.22. The yellowness b value was 18.45 for the cellulose dog gum of Comparative Example 1, 21.27 for the dog gum of Example 2, and 21.99 for the dog gum of Example 3, respectively. The ΔE value, which means the color difference between the overall brightness and saturation, was 50.45 for the cellulose dog gum of Comparative Example 1, 39.33 for the dog gum of Example 2, and 47.23 for the dog gum of Example 3, which was the highest in the cellulose dog gum.

Experimental Example 3: Analysis of the ability of dog gum composition to inhibit the formation of calculus and plaque

After 1 week of acclimatization in the laboratory, a total of 12 experimental beagle animals were anesthetized and scaled to remove calculus, and were left untreated for 4 weeks. After that, 6 dogs were ingested one per day for 4 weeks using the commercial cellulose dog gum of Comparative Example 1 and 6 dogs using the dog gum composition of Example 3, and then the degree of gingivitis, plaque, tartar, and feces were investigated.

The residual calculus content was evaluated qualitatively by staining method. area of investigation Measurements were made at 3 sites per tooth (distal buccal, mesial buccal, and buccal) of the left and right upper third incisors, upper and lower canines, upper and lower third and fourth premolars, and upper and lower first molars. After the progress evaluation of gingivitis, plaque and tartar evaluation at week 0, gingivitis, plaque and calculus were removed under anesthesia and feces were evaluated twice a week under anesthesia, and gingivitis, plaque and calculus were evaluated at 2 weeks and 4 weeks. and fecal evaluation twice a week.

The clinical index evaluated at this time is the gingival index (GI), the plaque index (PI), and the calculus index (CI), and the evaluation criteria are as follows. The data obtained in all experiments were verified for significance with the Multiple Mann-Whitney test, and these values were expressed as mean ± SD. did.

* Assessment of gingival inflammation (GI)

The gingival inflammation status was scored using the Silness and Lφe Cingival Index as follows.

0: normal teeth

1: mild inflammation; Mild color change, slightly swollen, inflamed gingiva that does not bleed with mild irritation

2: moderate inflammation; Inflamed gums with redness, swelling, and bleeding with mild irritation

3: severe inflammation; Inflammatory condition of the gingiva that has progressed to the point of marked redness, swelling, ulceration, and spontaneous bleeding

Gingival Inflammation Index (GI) = Sum of Total Scores / Total Number of Teeth Inspected

Judgment: 0.1-1.0 (mild), 1.1-2.0 (moderate), 2.1-3.0 (severe)

* Plaque assessment (CI)

Supragingival plaque coverage and plaque thickness were evaluated using the Turesky-Quigley-Hein Plaque index. Each tooth surface was stained with a red colorant (Erythrosin, sultan, USA) and scored in the following way. The score is according to Table 7 below.

Plaque index (PI) = sum of (coverage * thickness) / total number of teeth examined

* Tartar Assessment (PI)

Supragingival calculus coverage and calculus thickness were scored using the Warrick and Gorrel method as follows. The score is according to Table 8.

Gingival Inflammation Index (GI) = Sum of Total Scores / Total Number of Teeth Inspected

Judgment: 0.1-1.0 (mild), 1.1-2.0 (moderate), 2.1-3.0 (severe)

* Fecal evaluation

Fecal evaluation was performed twice a week according to the Waltham Faeces Scoring system. The Waltham Faeces Scoring system evaluation criteria, which are fecal evaluation criteria, are shown in FIG. 4 .

The measurement results of the gingival index (GI), plaque index (PI), and calculus index (CI) of the untreated control group that did not consume dog gum are shown in FIG. 5 .

According to this, the gingivitis (GI) index was measured as 0.37 at week 0, 1.08 at week 2, and 1.23 at week 4. The plaque index (PI) was measured as 0 at week 0, 2.96 at week 2, and 5.53 at week 4. The calculus index (CI) was measured as 0 at week 0, 1.94 at week 2, and 3.00 at week 4. That is, it was confirmed that the gingival inflammation index, plaque index, and calculus index significantly increased in the 2nd and 4th weeks compared to the 0th week in the control group that did not consume dog gum.

On the other hand, the gingival index (GI), plaque index (PI), and calculus index (CI) were measured in the teeth of the experimental beagle that consumed the commercial cellulose dog gum of Comparative Example 1 and the dog gum composition of Example 3, and the results are shown in FIG. indicated.

According to this, in the gingival index, the GI of the control group for the second week was measured to be 1.08, the GI of the dog gum intake group of Example 3 was 0.95, and the commercial dog gum intake group of Comparative Example 1 was measured to be 1.17. At the 4th week, the GI of the control group was measured to be 1.23, the dog gum intake group of Example 3 was measured to be 0.97, and the commercial dog gum intake group of Comparative Example 1 was measured to be 1.29. The gingival inflammation index of the dog gum ingestion group of Example 3 was lowered at both the 2nd and 4th weeks, and in particular, a significant decrease was observed at the 4th week as compared to the hors d'oeuvre control group and the commercial dog gum intake group of Comparative Example 1.

In addition, the plaque index was measured to be 2.96 in the second-week odor control group, 3.59 in the dog gum intake group of Example 3, and 3.56 in the commercial dog gum intake group of Comparative Example 1. At the 4th week, it was measured as 5.53 for the control group, 5.11 for the dog gum intake group of Example 3, and 6.24 for the general gum. The commercial dog gum intake group of Comparative Example 1 and the dog gum intake group of Example 3 exhibited similar plaque index at the 2nd week, but at the 4th week, the dog gum intake group of Example 3 showed a tendency to have a low plaque index.

The tartar index was measured as 1.94 for the control group without odor at the 2nd week, 1.82 for the dog gum intake group of Example 3, and 3.30 for the commercial dog gum intake group of Comparative Example 1. At the 4th week, it was measured to be 3.00 for the control group, 2.09 for the dog gum intake group of Example 3, and 3.50 for the commercial dog gum intake group of Comparative Example 1. The dog gum intake group of Example 3 exhibited the lowest calculus index at both the 2nd and 4th weeks compared to the odorless control group and the commercial dog gum intake group of Comparative Example 1.

On the other hand, untreated, commercial dog gum composition of Comparative Example 1, and 4 weeks after ingestion of the dog gum composition of Example 3, a photograph showing the state of teeth of the beagle is shown in FIG. When compared with the naked eye, when the dog gum composition of Example 3 of the present invention was ingested, the condition of the teeth appeared to be as clean as possible.

In addition, the result of evaluating feces according to the Waltham Faeces Scoring system is shown in FIG. 8 . There was no significant difference between the control group, the group that consumed the dog gum composition of Example 3 and the commercial dog gum composition of Comparative Example 1.

In the above, although embodiments of the present invention have been described, those of ordinary skill in the art can add, change, delete or add components within the scope that does not depart from the spirit of the present invention described in the claims. The present invention may be variously modified and changed by, etc., and this will also be included within the scope of the present invention.

Claims (17)

(a) heat-treating the crushed pear liquid, then filtering to remove the pear juice, and drying the by-product to obtain a pear pear powder;
(b) pulverizing the pear pear powder, and then selecting a size that passes through a 30 mesh mesh and does not pass through a 60 mesh mesh to obtain a screened pear pear powder;
(c) reacting the selected pear basil powder with alcohol to obtain a precipitate; and
(d) enzymatically treating the precipitate with Aspergillus niger-derived rapidase (Rapidase), removing the supernatant from the enzyme-treated product, and drying the precipitate with hot air; including;
In step (d), the Aspergillus niger-derived rapid Dase (Rapidase) is cellulase (cellulase), hemicellulase (hemicellulase), beta glucanase (β-glucanse) and pectinase ( pectinase),
A method for producing an abrasive composition for dog gum, wherein the insoluble dietary fiber content is 65 to 80% (w/v), and the stone cell content is 85 to 95% based on the total weight of the insoluble dietary fiber. The method of claim 1,
In step (a), the heat treatment method for producing an abrasive composition for dog gum, characterized in that performed at 100 to 140 ℃. delete The method of claim 1,
The alcohol is a method of producing an abrasive composition for dog gum, characterized in that the 40 to 50% ethanol aqueous solution. The method of claim 1,
In step (d), the Aspergillus niger-derived rapidase (Rapidase) is a method for producing an abrasive composition for dog gum, characterized in that the addition of 0.01 to 0.15 wt% of the solid content. delete The method of claim 1,
In step (d), the enzyme treatment is a method for producing an abrasive composition for dog gum, characterized in that it is performed for 8 to 20 hours at 35 to 40 ℃. The method of claim 1,
In step (d), the hot air drying method for producing an abrasive composition for dog gum, characterized in that performed for 10 to 20 hours at 50 to 70 ℃. It is prepared according to any one of claims 1, 2, 4, 5, 7 and 8, and the insoluble dietary fiber content is 65 to 80% (w/v), and the An abrasive composition for dog gum having a stone cell content of 85 to 95% based on the total weight of insoluble dietary fiber. 10. The method of claim 9,
The abrasive composition for dog gum is an abrasive composition for dog gum, characterized in that the total dietary fiber content is 75 to 85% (w/v). delete delete It is prepared according to any one of claims 1, 2, 4, 5, 7 and 8, and the insoluble dietary fiber content is 65 to 80% (w/v), and the A dog gum composition comprising an abrasive composition for dog gum having a stone cell content of 85 to 95% based on the total weight of insoluble dietary fiber. 14. The method of claim 13,
The dog gum composition comprises 5 to 20 wt% of the abrasive composition for dog gum based on the total weight. 14. The method of claim 13,
The dog gum composition has an acidity of 0.90 to 0.96% based on citric acid. 14. The method of claim 13,
The dog gum composition has a hardness of 11000 to 15000 g/cm ² Dog gum composition, characterized in that. 14. The method of claim 13,
The dog gum composition is a dog gum composition, characterized in that it is for removing or inhibiting gingivitis, plaque or tartar.

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