CN101978071A - 用于杂交核酸的方法 - Google Patents

用于杂交核酸的方法 Download PDF

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Publication number
CN101978071A
CN101978071A CN2008801227617A CN200880122761A CN101978071A CN 101978071 A CN101978071 A CN 101978071A CN 2008801227617 A CN2008801227617 A CN 2008801227617A CN 200880122761 A CN200880122761 A CN 200880122761A CN 101978071 A CN101978071 A CN 101978071A
Authority
CN
China
Prior art keywords
nucleic acid
oligonucleotide
target nucleic
molecule
oligocation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2008801227617A
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English (en)
Chinese (zh)
Inventor
N·勒纳
P·埃尔巴彻
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Université de Strasbourg
Polyplus Transfection SA
Original Assignee
Université de Strasbourg
Polyplus Transfection SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Université de Strasbourg, Polyplus Transfection SA filed Critical Université de Strasbourg
Publication of CN101978071A publication Critical patent/CN101978071A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2008801227617A 2007-12-27 2008-09-12 用于杂交核酸的方法 Pending CN101978071A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US914507P 2007-12-27 2007-12-27
US61/009,145 2007-12-27
EP07025148A EP2075342A1 (en) 2007-12-27 2007-12-27 Method for hybridizing nucleic acids
EP07025148.3 2007-12-27
PCT/IB2008/002789 WO2009083763A1 (en) 2007-12-27 2008-09-12 Method for hybridizing nucleic acids

Publications (1)

Publication Number Publication Date
CN101978071A true CN101978071A (zh) 2011-02-16

Family

ID=39339663

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2008801227617A Pending CN101978071A (zh) 2007-12-27 2008-09-12 用于杂交核酸的方法

Country Status (14)

Country Link
US (1) US8465920B2 (enExample)
EP (2) EP2075342A1 (enExample)
JP (1) JP2011507542A (enExample)
KR (1) KR20100124705A (enExample)
CN (1) CN101978071A (enExample)
AU (1) AU2008345460A1 (enExample)
BR (1) BRPI0821698A2 (enExample)
CA (1) CA2709183A1 (enExample)
DK (1) DK2225393T3 (enExample)
ES (1) ES2537625T3 (enExample)
IL (1) IL206542A0 (enExample)
NZ (1) NZ586373A (enExample)
RU (1) RU2010131166A (enExample)
WO (1) WO2009083763A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113423842A (zh) * 2018-10-22 2021-09-21 柏业公司 具有与之结合的八胺或八胺衍生物的探针及其用途
WO2021189574A1 (zh) * 2020-03-27 2021-09-30 广州达安基因股份有限公司 具有高杂交性能的寡核苷酸缀合物及其应用

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3214174B1 (en) 2010-03-04 2019-10-16 InteRNA Technologies B.V. A mirna molecule defined by its source and its diagnostic and therapeutic uses in diseases or conditions associated with emt
EP2591106A1 (en) 2010-07-06 2013-05-15 InteRNA Technologies B.V. Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway
JP5795317B2 (ja) * 2010-09-10 2015-10-14 株式会社Ｌｓｉメディエンス 光を用いた核酸の増幅抑制方法および高感度な選択的核酸増幅方法
EP2474617A1 (en) 2011-01-11 2012-07-11 InteRNA Technologies BV Mir for treating neo-angiogenesis
EP3369818B1 (en) 2011-12-22 2021-06-09 InteRNA Technologies B.V. Mirna for treating head and neck cancer
KR102146523B1 (ko) * 2012-01-06 2020-08-20 조원창 표적 핵산의 향상된 증폭
US10201556B2 (en) 2012-11-06 2019-02-12 Interna Technologies B.V. Combination for use in treating diseases or conditions associated with melanoma, or treating diseases or conditions associated with activated B-raf pathway
US9410172B2 (en) * 2013-09-16 2016-08-09 General Electric Company Isothermal amplification using oligocation-conjugated primer sequences
DE102017123919A1 (de) 2017-10-13 2019-04-18 Gna Biosolutions Gmbh Verfahren und Vorrichtung zur Lyse von Mikroorganismen
AU2018358016A1 (en) 2017-11-03 2020-05-07 Interna Technologies B.V. MiRNA molecule, equivalent, antagomir, or source thereof for treating and/or diagnosing a condition and/or a disease associated with neuronal deficiency or for neuronal (re)generation
WO2019155094A1 (en) 2018-02-12 2019-08-15 Interna Technologies B.V. Anticancer microrna and lipid formulations thereof
IT202200026595A1 (it) 2022-12-22 2024-06-22 Fond Telethon Ets Nuovi inibitori di regolatori epigenetici
WO2026133078A1 (en) 2024-12-17 2026-06-25 Fondazione Telethon Ets Therapeutic strategy for treating copper-mediated pathologies

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007069092A2 (en) * 2005-12-15 2007-06-21 Centre National De La Recherche Scientifique (Cnrs) Cationic oligonucleotides, automated methods for preparing same and their uses

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1013770A1 (en) * 1998-12-23 2000-06-28 Université Louis Pasteur de Strasbourg Non-viral transfection vector
JP2008519053A (ja) * 2004-11-04 2008-06-05 アンディーノ，ラウル 小さい干渉ＲＮＡ（ｓｉＲＮＡ）のポリアミン共役体の合成及びそれによって形成された共役体

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007069092A2 (en) * 2005-12-15 2007-06-21 Centre National De La Recherche Scientifique (Cnrs) Cationic oligonucleotides, automated methods for preparing same and their uses

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113423842A (zh) * 2018-10-22 2021-09-21 柏业公司 具有与之结合的八胺或八胺衍生物的探针及其用途
WO2021189574A1 (zh) * 2020-03-27 2021-09-30 广州达安基因股份有限公司 具有高杂交性能的寡核苷酸缀合物及其应用
US12331355B2 (en) 2020-03-27 2025-06-17 Daan Gene Co., Ltd. Oligonucleotide conjugate with high hybridization performance and use thereof

Also Published As

Publication number Publication date
NZ586373A (en) 2012-09-28
AU2008345460A1 (en) 2009-07-09
KR20100124705A (ko) 2010-11-29
US8465920B2 (en) 2013-06-18
US20100311056A1 (en) 2010-12-09
JP2011507542A (ja) 2011-03-10
ES2537625T3 (es) 2015-06-10
IL206542A0 (en) 2010-12-30
DK2225393T3 (en) 2015-05-26
RU2010131166A (ru) 2012-02-10
EP2225393A1 (en) 2010-09-08
BRPI0821698A2 (pt) 2015-06-16
CA2709183A1 (en) 2009-07-09
EP2075342A1 (en) 2009-07-01
WO2009083763A1 (en) 2009-07-09
EP2225393B1 (en) 2015-02-25

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Application publication date: 20110216