DK2225393T3 - A method for hybridization of nucleic acids - Google Patents

A method for hybridization of nucleic acids Download PDF

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Publication number
DK2225393T3
DK2225393T3 DK08868164.8T DK08868164T DK2225393T3 DK 2225393 T3 DK2225393 T3 DK 2225393T3 DK 08868164 T DK08868164 T DK 08868164T DK 2225393 T3 DK2225393 T3 DK 2225393T3
Authority
DK
Denmark
Prior art keywords
oligonucleotide
probe
conjugate
oligocation
nucleic acid
Prior art date
Application number
DK08868164.8T
Other languages
Danish (da)
English (en)
Inventor
Patrick Erbacher
Nathalie Lenne
Original Assignee
Polyplus Transfection
Univ Strasbourg
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Polyplus Transfection, Univ Strasbourg, Centre Nat Rech Scient filed Critical Polyplus Transfection
Application granted granted Critical
Publication of DK2225393T3 publication Critical patent/DK2225393T3/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK08868164.8T 2007-12-27 2008-09-12 A method for hybridization of nucleic acids DK2225393T3 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US914507P 2007-12-27 2007-12-27
EP07025148A EP2075342A1 (en) 2007-12-27 2007-12-27 Method for hybridizing nucleic acids
PCT/IB2008/002789 WO2009083763A1 (en) 2007-12-27 2008-09-12 Method for hybridizing nucleic acids

Publications (1)

Publication Number Publication Date
DK2225393T3 true DK2225393T3 (en) 2015-05-26

Family

ID=39339663

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08868164.8T DK2225393T3 (en) 2007-12-27 2008-09-12 A method for hybridization of nucleic acids

Country Status (14)

Country Link
US (1) US8465920B2 (enExample)
EP (2) EP2075342A1 (enExample)
JP (1) JP2011507542A (enExample)
KR (1) KR20100124705A (enExample)
CN (1) CN101978071A (enExample)
AU (1) AU2008345460A1 (enExample)
BR (1) BRPI0821698A2 (enExample)
CA (1) CA2709183A1 (enExample)
DK (1) DK2225393T3 (enExample)
ES (1) ES2537625T3 (enExample)
IL (1) IL206542A0 (enExample)
NZ (1) NZ586373A (enExample)
RU (1) RU2010131166A (enExample)
WO (1) WO2009083763A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2631458T3 (es) 2010-03-04 2017-08-31 Interna Technologies B.V. Molécula de ARNmi definida por su fuente y sus usos terapéuticos en el cáncer asociado a la EMT
EP3369817A1 (en) 2010-07-06 2018-09-05 InteRNA Technologies B.V. Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma , or in diseases or conditions with activated braf pathway
EP2615171B1 (en) * 2010-09-10 2015-11-04 LSI Medience Corporation Method for inhibiting nucleic acid amplification using light and highly sensitive method for selective nucleic acid amplification
EP2474617A1 (en) 2011-01-11 2012-07-11 InteRNA Technologies BV Mir for treating neo-angiogenesis
US10174314B2 (en) 2011-12-22 2019-01-08 Interna Technologies B.V. MiRNA for treating head and neck cancer
KR102146523B1 (ko) * 2012-01-06 2020-08-20 조원창 표적 핵산의 향상된 증폭
US10201556B2 (en) 2012-11-06 2019-02-12 Interna Technologies B.V. Combination for use in treating diseases or conditions associated with melanoma, or treating diseases or conditions associated with activated B-raf pathway
US9410172B2 (en) * 2013-09-16 2016-08-09 General Electric Company Isothermal amplification using oligocation-conjugated primer sequences
DE102017123919A1 (de) 2017-10-13 2019-04-18 Gna Biosolutions Gmbh Verfahren und Vorrichtung zur Lyse von Mikroorganismen
AU2018358016A1 (en) 2017-11-03 2020-05-07 Interna Technologies B.V. MiRNA molecule, equivalent, antagomir, or source thereof for treating and/or diagnosing a condition and/or a disease associated with neuronal deficiency or for neuronal (re)generation
CN111936150A (zh) 2018-02-12 2020-11-13 因特尔纳技术有限公司 抗癌微小rna及其脂质制剂
KR20200045212A (ko) * 2018-10-22 2020-05-04 (주)바이오니아 옥타민 또는 옥타민 유도체가 결합된 프로브 및 이의 용도
CN113444778A (zh) * 2020-03-27 2021-09-28 广州达安基因股份有限公司 具有高杂交性能的寡核苷酸缀合物及其应用
IT202200026595A1 (it) 2022-12-22 2024-06-22 Fond Telethon Ets Nuovi inibitori di regolatori epigenetici

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1013770A1 (en) * 1998-12-23 2000-06-28 Université Louis Pasteur de Strasbourg Non-viral transfection vector
JP2008519053A (ja) * 2004-11-04 2008-06-05 アンディーノ,ラウル 小さい干渉RNA(siRNA)のポリアミン共役体の合成及びそれによって形成された共役体
AU2006325054B2 (en) 2005-12-15 2013-05-02 Centre National De La Recherche Scientifique (Cnrs) Cationic oligonucleotides, automated methods for preparing same and their uses

Also Published As

Publication number Publication date
RU2010131166A (ru) 2012-02-10
CA2709183A1 (en) 2009-07-09
EP2075342A1 (en) 2009-07-01
IL206542A0 (en) 2010-12-30
US8465920B2 (en) 2013-06-18
NZ586373A (en) 2012-09-28
KR20100124705A (ko) 2010-11-29
EP2225393B1 (en) 2015-02-25
EP2225393A1 (en) 2010-09-08
BRPI0821698A2 (pt) 2015-06-16
AU2008345460A1 (en) 2009-07-09
JP2011507542A (ja) 2011-03-10
ES2537625T3 (es) 2015-06-10
CN101978071A (zh) 2011-02-16
WO2009083763A1 (en) 2009-07-09
US20100311056A1 (en) 2010-12-09

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