RU2010131166A - Способ гибридизации нуклеиновых кислот - Google Patents
Способ гибридизации нуклеиновых кислот Download PDFInfo
- Publication number
- RU2010131166A RU2010131166A RU2010131166/10A RU2010131166A RU2010131166A RU 2010131166 A RU2010131166 A RU 2010131166A RU 2010131166/10 A RU2010131166/10 A RU 2010131166/10A RU 2010131166 A RU2010131166 A RU 2010131166A RU 2010131166 A RU2010131166 A RU 2010131166A
- Authority
- RU
- Russia
- Prior art keywords
- nucleic acid
- oligonucleotide
- conjugate
- target nucleic
- oligocationic
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title claims abstract 21
- 102000039446 nucleic acids Human genes 0.000 title claims abstract 21
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract 21
- 238000000034 method Methods 0.000 title claims abstract 19
- 238000009396 hybridization Methods 0.000 title 1
- 239000012634 fragment Substances 0.000 claims abstract 7
- 125000005647 linker group Chemical group 0.000 claims abstract 5
- 239000003550 marker Substances 0.000 claims abstract 4
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical group NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 claims abstract 4
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical group NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 claims abstract 4
- 238000001514 detection method Methods 0.000 claims abstract 3
- WSNMPAVSZJSIMT-UHFFFAOYSA-N COc1c(C)c2COC(=O)c2c(O)c1CC(O)C1(C)CCC(=O)O1 Chemical compound COc1c(C)c2COC(=O)c2c(O)c1CC(O)C1(C)CCC(=O)O1 WSNMPAVSZJSIMT-UHFFFAOYSA-N 0.000 claims abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 claims abstract 2
- 239000005700 Putrescine Substances 0.000 claims abstract 2
- 229940063673 spermidine Drugs 0.000 claims abstract 2
- PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical group NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 claims abstract 2
- 238000004458 analytical method Methods 0.000 claims 8
- 239000000523 sample Substances 0.000 claims 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- 239000000427 antigen Substances 0.000 claims 2
- 102000036639 antigens Human genes 0.000 claims 2
- 108091007433 antigens Proteins 0.000 claims 2
- 229960002685 biotin Drugs 0.000 claims 2
- 235000020958 biotin Nutrition 0.000 claims 2
- 239000011616 biotin Substances 0.000 claims 2
- 150000001841 cholesterols Chemical class 0.000 claims 2
- 230000002209 hydrophobic effect Effects 0.000 claims 2
- 239000002245 particle Substances 0.000 claims 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 238000010839 reverse transcription Methods 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- 230000002194 synthesizing effect Effects 0.000 claims 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 1
- 108091028664 Ribonucleotide Proteins 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000005547 deoxyribonucleotide Substances 0.000 claims 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 1
- 239000000834 fixative Substances 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000001668 nucleic acid synthesis Methods 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 238000003753 real-time PCR Methods 0.000 claims 1
- 239000002336 ribonucleotide Substances 0.000 claims 1
- 125000002652 ribonucleotide group Chemical group 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US914507P | 2007-12-27 | 2007-12-27 | |
| EP07025148A EP2075342A1 (en) | 2007-12-27 | 2007-12-27 | Method for hybridizing nucleic acids |
| EP07025148.3 | 2007-12-27 | ||
| US61/009,145 | 2007-12-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2010131166A true RU2010131166A (ru) | 2012-02-10 |
Family
ID=39339663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2010131166/10A RU2010131166A (ru) | 2007-12-27 | 2008-09-12 | Способ гибридизации нуклеиновых кислот |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US8465920B2 (enExample) |
| EP (2) | EP2075342A1 (enExample) |
| JP (1) | JP2011507542A (enExample) |
| KR (1) | KR20100124705A (enExample) |
| CN (1) | CN101978071A (enExample) |
| AU (1) | AU2008345460A1 (enExample) |
| BR (1) | BRPI0821698A2 (enExample) |
| CA (1) | CA2709183A1 (enExample) |
| DK (1) | DK2225393T3 (enExample) |
| ES (1) | ES2537625T3 (enExample) |
| IL (1) | IL206542A0 (enExample) |
| NZ (1) | NZ586373A (enExample) |
| RU (1) | RU2010131166A (enExample) |
| WO (1) | WO2009083763A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011108930A1 (en) | 2010-03-04 | 2011-09-09 | Interna Technologies Bv | A MiRNA MOLECULE DEFINED BY ITS SOURCE AND ITS DIAGNOSTIC AND THERAPEUTIC USES IN DISEASES OR CONDITIONS ASSOCIATED WITH EMT |
| EP2591106A1 (en) | 2010-07-06 | 2013-05-15 | InteRNA Technologies B.V. | Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway |
| CN103080310A (zh) * | 2010-09-10 | 2013-05-01 | 三菱化学美迪恩斯株式会社 | 使用光的核酸的扩增抑制方法和高灵敏度的选择性核酸扩增方法 |
| EP2474617A1 (en) | 2011-01-11 | 2012-07-11 | InteRNA Technologies BV | Mir for treating neo-angiogenesis |
| WO2013095132A1 (en) | 2011-12-22 | 2013-06-27 | Interna Technologies B.V. | Mirna for treating head and neck cancer |
| KR102146523B1 (ko) * | 2012-01-06 | 2020-08-20 | 조원창 | 표적 핵산의 향상된 증폭 |
| WO2014072357A1 (en) | 2012-11-06 | 2014-05-15 | Interna Technologies B.V. | Combination for use in treating diseases or conditions associated with melanoma, or treating diseases or conditions associated with activated b-raf pathway |
| US9410172B2 (en) * | 2013-09-16 | 2016-08-09 | General Electric Company | Isothermal amplification using oligocation-conjugated primer sequences |
| DE102017123919A1 (de) | 2017-10-13 | 2019-04-18 | Gna Biosolutions Gmbh | Verfahren und Vorrichtung zur Lyse von Mikroorganismen |
| CN111566212A (zh) | 2017-11-03 | 2020-08-21 | 因特尔纳技术有限公司 | miRNA分子，等同物，安塔够妙或其来源用于治疗和/或诊断与神经元缺陷相关的病症和/或疾病或用于神经元生成和/或再生 |
| JP2021513508A (ja) | 2018-02-12 | 2021-05-27 | インテアールエヌエー テクノロジーズ ビー．ヴイ．ＩｎｔｅＲＮＡ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｂ．Ｖ． | 抗がんマイクロｒｎａ及びその脂質製剤 |
| KR20200045212A (ko) | 2018-10-22 | 2020-05-04 | (주)바이오니아 | 옥타민 또는 옥타민 유도체가 결합된 프로브 및 이의 용도 |
| CN113444778A (zh) | 2020-03-27 | 2021-09-28 | 广州达安基因股份有限公司 | 具有高杂交性能的寡核苷酸缀合物及其应用 |
| IT202200026595A1 (it) | 2022-12-22 | 2024-06-22 | Fond Telethon Ets | Nuovi inibitori di regolatori epigenetici |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1013770A1 (en) * | 1998-12-23 | 2000-06-28 | Université Louis Pasteur de Strasbourg | Non-viral transfection vector |
| CA2581869A1 (en) * | 2004-11-04 | 2006-05-18 | Raul Andino | Syntheses of polyamine conjugates of small interfering rnas (si-rnas) and conjugates formed thereby |
| BRPI0619932A2 (pt) | 2005-12-15 | 2011-10-25 | Centre Nat Rech Scient | moléculas de oligonucleotìdeo-oligocátion, método para a obtenção das mesmas, reagentes de fosforamidita, método para uso em biologia e diagnóstico, e, composições farmacêuticas |
-
2007
- 2007-12-27 EP EP07025148A patent/EP2075342A1/en not_active Withdrawn
-
2008
- 2008-09-12 EP EP08868164.8A patent/EP2225393B1/en active Active
- 2008-09-12 RU RU2010131166/10A patent/RU2010131166A/ru not_active Application Discontinuation
- 2008-09-12 AU AU2008345460A patent/AU2008345460A1/en not_active Abandoned
- 2008-09-12 US US12/735,261 patent/US8465920B2/en active Active
- 2008-09-12 BR BRPI0821698-3A patent/BRPI0821698A2/pt not_active IP Right Cessation
- 2008-09-12 DK DK08868164.8T patent/DK2225393T3/en active
- 2008-09-12 JP JP2010540181A patent/JP2011507542A/ja active Pending
- 2008-09-12 CA CA2709183A patent/CA2709183A1/en not_active Abandoned
- 2008-09-12 ES ES08868164.8T patent/ES2537625T3/es active Active
- 2008-09-12 WO PCT/IB2008/002789 patent/WO2009083763A1/en not_active Ceased
- 2008-09-12 NZ NZ586373A patent/NZ586373A/xx not_active IP Right Cessation
- 2008-09-12 CN CN2008801227617A patent/CN101978071A/zh active Pending
- 2008-09-12 KR KR1020107015189A patent/KR20100124705A/ko not_active Withdrawn
-
2010
- 2010-06-22 IL IL206542A patent/IL206542A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IL206542A0 (en) | 2010-12-30 |
| AU2008345460A1 (en) | 2009-07-09 |
| US8465920B2 (en) | 2013-06-18 |
| CA2709183A1 (en) | 2009-07-09 |
| CN101978071A (zh) | 2011-02-16 |
| BRPI0821698A2 (pt) | 2015-06-16 |
| EP2075342A1 (en) | 2009-07-01 |
| WO2009083763A1 (en) | 2009-07-09 |
| JP2011507542A (ja) | 2011-03-10 |
| EP2225393A1 (en) | 2010-09-08 |
| EP2225393B1 (en) | 2015-02-25 |
| NZ586373A (en) | 2012-09-28 |
| DK2225393T3 (en) | 2015-05-26 |
| US20100311056A1 (en) | 2010-12-09 |
| KR20100124705A (ko) | 2010-11-29 |
| ES2537625T3 (es) | 2015-06-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20130603 |