RU2010131166A - Способ гибридизации нуклеиновых кислот - Google Patents
Способ гибридизации нуклеиновых кислот Download PDFInfo
- Publication number
- RU2010131166A RU2010131166A RU2010131166/10A RU2010131166A RU2010131166A RU 2010131166 A RU2010131166 A RU 2010131166A RU 2010131166/10 A RU2010131166/10 A RU 2010131166/10A RU 2010131166 A RU2010131166 A RU 2010131166A RU 2010131166 A RU2010131166 A RU 2010131166A
- Authority
- RU
- Russia
- Prior art keywords
- nucleic acid
- oligonucleotide
- conjugate
- target nucleic
- oligocationic
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title claims abstract 21
- 102000039446 nucleic acids Human genes 0.000 title claims abstract 21
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract 21
- 238000000034 method Methods 0.000 title claims abstract 19
- 238000009396 hybridization Methods 0.000 title 1
- 239000012634 fragment Substances 0.000 claims abstract 7
- 125000005647 linker group Chemical group 0.000 claims abstract 5
- 239000003550 marker Substances 0.000 claims abstract 4
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical group NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 claims abstract 4
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical group NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 claims abstract 4
- 238000001514 detection method Methods 0.000 claims abstract 3
- WSNMPAVSZJSIMT-UHFFFAOYSA-N COc1c(C)c2COC(=O)c2c(O)c1CC(O)C1(C)CCC(=O)O1 Chemical compound COc1c(C)c2COC(=O)c2c(O)c1CC(O)C1(C)CCC(=O)O1 WSNMPAVSZJSIMT-UHFFFAOYSA-N 0.000 claims abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 claims abstract 2
- 239000005700 Putrescine Substances 0.000 claims abstract 2
- 229940063673 spermidine Drugs 0.000 claims abstract 2
- PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical group NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 claims abstract 2
- 238000004458 analytical method Methods 0.000 claims 8
- 239000000523 sample Substances 0.000 claims 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- 239000000427 antigen Substances 0.000 claims 2
- 102000036639 antigens Human genes 0.000 claims 2
- 108091007433 antigens Proteins 0.000 claims 2
- 229960002685 biotin Drugs 0.000 claims 2
- 235000020958 biotin Nutrition 0.000 claims 2
- 239000011616 biotin Substances 0.000 claims 2
- 150000001841 cholesterols Chemical class 0.000 claims 2
- 230000002209 hydrophobic effect Effects 0.000 claims 2
- 239000002245 particle Substances 0.000 claims 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 238000010839 reverse transcription Methods 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- 230000002194 synthesizing effect Effects 0.000 claims 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 1
- 108091028664 Ribonucleotide Proteins 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000005547 deoxyribonucleotide Substances 0.000 claims 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 1
- 239000000834 fixative Substances 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000001668 nucleic acid synthesis Methods 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 238000003753 real-time PCR Methods 0.000 claims 1
- 239000002336 ribonucleotide Substances 0.000 claims 1
- 125000002652 ribonucleotide group Chemical group 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US914507P | 2007-12-27 | 2007-12-27 | |
| US61/009,145 | 2007-12-27 | ||
| EP07025148A EP2075342A1 (en) | 2007-12-27 | 2007-12-27 | Method for hybridizing nucleic acids |
| EP07025148.3 | 2007-12-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2010131166A true RU2010131166A (ru) | 2012-02-10 |
Family
ID=39339663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2010131166/10A RU2010131166A (ru) | 2007-12-27 | 2008-09-12 | Способ гибридизации нуклеиновых кислот |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US8465920B2 (enExample) |
| EP (2) | EP2075342A1 (enExample) |
| JP (1) | JP2011507542A (enExample) |
| KR (1) | KR20100124705A (enExample) |
| CN (1) | CN101978071A (enExample) |
| AU (1) | AU2008345460A1 (enExample) |
| BR (1) | BRPI0821698A2 (enExample) |
| CA (1) | CA2709183A1 (enExample) |
| DK (1) | DK2225393T3 (enExample) |
| ES (1) | ES2537625T3 (enExample) |
| IL (1) | IL206542A0 (enExample) |
| NZ (1) | NZ586373A (enExample) |
| RU (1) | RU2010131166A (enExample) |
| WO (1) | WO2009083763A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3214174B1 (en) | 2010-03-04 | 2019-10-16 | InteRNA Technologies B.V. | A mirna molecule defined by its source and its diagnostic and therapeutic uses in diseases or conditions associated with emt |
| EP2591106A1 (en) | 2010-07-06 | 2013-05-15 | InteRNA Technologies B.V. | Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway |
| JP5795317B2 (ja) * | 2010-09-10 | 2015-10-14 | 株式会社Ｌｓｉメディエンス | 光を用いた核酸の増幅抑制方法および高感度な選択的核酸増幅方法 |
| EP2474617A1 (en) | 2011-01-11 | 2012-07-11 | InteRNA Technologies BV | Mir for treating neo-angiogenesis |
| EP3369818B1 (en) | 2011-12-22 | 2021-06-09 | InteRNA Technologies B.V. | Mirna for treating head and neck cancer |
| KR102146523B1 (ko) * | 2012-01-06 | 2020-08-20 | 조원창 | 표적 핵산의 향상된 증폭 |
| US10201556B2 (en) | 2012-11-06 | 2019-02-12 | Interna Technologies B.V. | Combination for use in treating diseases or conditions associated with melanoma, or treating diseases or conditions associated with activated B-raf pathway |
| US9410172B2 (en) * | 2013-09-16 | 2016-08-09 | General Electric Company | Isothermal amplification using oligocation-conjugated primer sequences |
| DE102017123919A1 (de) | 2017-10-13 | 2019-04-18 | Gna Biosolutions Gmbh | Verfahren und Vorrichtung zur Lyse von Mikroorganismen |
| AU2018358016A1 (en) | 2017-11-03 | 2020-05-07 | Interna Technologies B.V. | MiRNA molecule, equivalent, antagomir, or source thereof for treating and/or diagnosing a condition and/or a disease associated with neuronal deficiency or for neuronal (re)generation |
| WO2019155094A1 (en) | 2018-02-12 | 2019-08-15 | Interna Technologies B.V. | Anticancer microrna and lipid formulations thereof |
| KR20200045212A (ko) | 2018-10-22 | 2020-05-04 | (주)바이오니아 | 옥타민 또는 옥타민 유도체가 결합된 프로브 및 이의 용도 |
| CN113444778A (zh) | 2020-03-27 | 2021-09-28 | 广州达安基因股份有限公司 | 具有高杂交性能的寡核苷酸缀合物及其应用 |
| IT202200026595A1 (it) | 2022-12-22 | 2024-06-22 | Fond Telethon Ets | Nuovi inibitori di regolatori epigenetici |
| WO2026133078A1 (en) | 2024-12-17 | 2026-06-25 | Fondazione Telethon Ets | Therapeutic strategy for treating copper-mediated pathologies |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1013770A1 (en) * | 1998-12-23 | 2000-06-28 | Université Louis Pasteur de Strasbourg | Non-viral transfection vector |
| JP2008519053A (ja) * | 2004-11-04 | 2008-06-05 | アンディーノ，ラウル | 小さい干渉ＲＮＡ（ｓｉＲＮＡ）のポリアミン共役体の合成及びそれによって形成された共役体 |
| KR101388320B1 (ko) | 2005-12-15 | 2014-04-22 | 뽈리쁠뤼스-트랑스펙씨옹 | 양이온성 올리고뉴클레오티드, 이를 제조하기 위한 자동화방법 및 이의 용도 |
-
2007
- 2007-12-27 EP EP07025148A patent/EP2075342A1/en not_active Withdrawn
-
2008
- 2008-09-12 US US12/735,261 patent/US8465920B2/en active Active
- 2008-09-12 BR BRPI0821698-3A patent/BRPI0821698A2/pt not_active IP Right Cessation
- 2008-09-12 ES ES08868164.8T patent/ES2537625T3/es active Active
- 2008-09-12 KR KR1020107015189A patent/KR20100124705A/ko not_active Withdrawn
- 2008-09-12 AU AU2008345460A patent/AU2008345460A1/en not_active Abandoned
- 2008-09-12 CN CN2008801227617A patent/CN101978071A/zh active Pending
- 2008-09-12 JP JP2010540181A patent/JP2011507542A/ja active Pending
- 2008-09-12 CA CA2709183A patent/CA2709183A1/en not_active Abandoned
- 2008-09-12 EP EP08868164.8A patent/EP2225393B1/en active Active
- 2008-09-12 NZ NZ586373A patent/NZ586373A/xx not_active IP Right Cessation
- 2008-09-12 RU RU2010131166/10A patent/RU2010131166A/ru not_active Application Discontinuation
- 2008-09-12 WO PCT/IB2008/002789 patent/WO2009083763A1/en not_active Ceased
- 2008-09-12 DK DK08868164.8T patent/DK2225393T3/en active
-
2010
- 2010-06-22 IL IL206542A patent/IL206542A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NZ586373A (en) | 2012-09-28 |
| AU2008345460A1 (en) | 2009-07-09 |
| KR20100124705A (ko) | 2010-11-29 |
| US8465920B2 (en) | 2013-06-18 |
| US20100311056A1 (en) | 2010-12-09 |
| JP2011507542A (ja) | 2011-03-10 |
| ES2537625T3 (es) | 2015-06-10 |
| CN101978071A (zh) | 2011-02-16 |
| IL206542A0 (en) | 2010-12-30 |
| DK2225393T3 (en) | 2015-05-26 |
| EP2225393A1 (en) | 2010-09-08 |
| BRPI0821698A2 (pt) | 2015-06-16 |
| CA2709183A1 (en) | 2009-07-09 |
| EP2075342A1 (en) | 2009-07-01 |
| WO2009083763A1 (en) | 2009-07-09 |
| EP2225393B1 (en) | 2015-02-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20130603 |