ES2537625T3 - Método para hibridar ácidos nucleicos - Google Patents

Método para hibridar ácidos nucleicos Download PDF

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Publication number
ES2537625T3
ES2537625T3 ES08868164.8T ES08868164T ES2537625T3 ES 2537625 T3 ES2537625 T3 ES 2537625T3 ES 08868164 T ES08868164 T ES 08868164T ES 2537625 T3 ES2537625 T3 ES 2537625T3
Authority
ES
Spain
Prior art keywords
primers
conjugate
different
oligonucleotide
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08868164.8T
Other languages
English (en)
Spanish (es)
Inventor
Nathalie Lenne
Patrick Erbacher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique
Université de Strasbourg
Polyplus Transfection SA
Original Assignee
Centre National de la Recherche Scientifique
Université de Strasbourg
Polyplus Transfection SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique, Université de Strasbourg, Polyplus Transfection SA filed Critical Centre National de la Recherche Scientifique
Application granted granted Critical
Publication of ES2537625T3 publication Critical patent/ES2537625T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES08868164.8T 2007-12-27 2008-09-12 Método para hibridar ácidos nucleicos Active ES2537625T3 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US9145P 1995-12-22
US914507P 2007-12-27 2007-12-27
EP07025148 2007-12-27
EP07025148A EP2075342A1 (en) 2007-12-27 2007-12-27 Method for hybridizing nucleic acids
PCT/IB2008/002789 WO2009083763A1 (en) 2007-12-27 2008-09-12 Method for hybridizing nucleic acids

Publications (1)

Publication Number Publication Date
ES2537625T3 true ES2537625T3 (es) 2015-06-10

Family

ID=39339663

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08868164.8T Active ES2537625T3 (es) 2007-12-27 2008-09-12 Método para hibridar ácidos nucleicos

Country Status (14)

Country Link
US (1) US8465920B2 (enExample)
EP (2) EP2075342A1 (enExample)
JP (1) JP2011507542A (enExample)
KR (1) KR20100124705A (enExample)
CN (1) CN101978071A (enExample)
AU (1) AU2008345460A1 (enExample)
BR (1) BRPI0821698A2 (enExample)
CA (1) CA2709183A1 (enExample)
DK (1) DK2225393T3 (enExample)
ES (1) ES2537625T3 (enExample)
IL (1) IL206542A0 (enExample)
NZ (1) NZ586373A (enExample)
RU (1) RU2010131166A (enExample)
WO (1) WO2009083763A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3214174B1 (en) 2010-03-04 2019-10-16 InteRNA Technologies B.V. A mirna molecule defined by its source and its diagnostic and therapeutic uses in diseases or conditions associated with emt
EP2591106A1 (en) 2010-07-06 2013-05-15 InteRNA Technologies B.V. Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway
JP5795317B2 (ja) * 2010-09-10 2015-10-14 株式会社Ｌｓｉメディエンス 光を用いた核酸の増幅抑制方法および高感度な選択的核酸増幅方法
EP2474617A1 (en) 2011-01-11 2012-07-11 InteRNA Technologies BV Mir for treating neo-angiogenesis
EP3369818B1 (en) 2011-12-22 2021-06-09 InteRNA Technologies B.V. Mirna for treating head and neck cancer
KR102146523B1 (ko) * 2012-01-06 2020-08-20 조원창 표적 핵산의 향상된 증폭
US10201556B2 (en) 2012-11-06 2019-02-12 Interna Technologies B.V. Combination for use in treating diseases or conditions associated with melanoma, or treating diseases or conditions associated with activated B-raf pathway
US9410172B2 (en) * 2013-09-16 2016-08-09 General Electric Company Isothermal amplification using oligocation-conjugated primer sequences
DE102017123919A1 (de) 2017-10-13 2019-04-18 Gna Biosolutions Gmbh Verfahren und Vorrichtung zur Lyse von Mikroorganismen
AU2018358016A1 (en) 2017-11-03 2020-05-07 Interna Technologies B.V. MiRNA molecule, equivalent, antagomir, or source thereof for treating and/or diagnosing a condition and/or a disease associated with neuronal deficiency or for neuronal (re)generation
WO2019155094A1 (en) 2018-02-12 2019-08-15 Interna Technologies B.V. Anticancer microrna and lipid formulations thereof
KR20200045212A (ko) 2018-10-22 2020-05-04 (주)바이오니아 옥타민 또는 옥타민 유도체가 결합된 프로브 및 이의 용도
CN113444778A (zh) 2020-03-27 2021-09-28 广州达安基因股份有限公司 具有高杂交性能的寡核苷酸缀合物及其应用
IT202200026595A1 (it) 2022-12-22 2024-06-22 Fond Telethon Ets Nuovi inibitori di regolatori epigenetici
WO2026133078A1 (en) 2024-12-17 2026-06-25 Fondazione Telethon Ets Therapeutic strategy for treating copper-mediated pathologies

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1013770A1 (en) * 1998-12-23 2000-06-28 Université Louis Pasteur de Strasbourg Non-viral transfection vector
JP2008519053A (ja) * 2004-11-04 2008-06-05 アンディーノ，ラウル 小さい干渉ＲＮＡ（ｓｉＲＮＡ）のポリアミン共役体の合成及びそれによって形成された共役体
KR101388320B1 (ko) 2005-12-15 2014-04-22 뽈리쁠뤼스-트랑스펙씨옹 양이온성 올리고뉴클레오티드, 이를 제조하기 위한 자동화방법 및 이의 용도

Also Published As

Publication number Publication date
NZ586373A (en) 2012-09-28
AU2008345460A1 (en) 2009-07-09
KR20100124705A (ko) 2010-11-29
US8465920B2 (en) 2013-06-18
US20100311056A1 (en) 2010-12-09
JP2011507542A (ja) 2011-03-10
CN101978071A (zh) 2011-02-16
IL206542A0 (en) 2010-12-30
DK2225393T3 (en) 2015-05-26
RU2010131166A (ru) 2012-02-10
EP2225393A1 (en) 2010-09-08
BRPI0821698A2 (pt) 2015-06-16
CA2709183A1 (en) 2009-07-09
EP2075342A1 (en) 2009-07-01
WO2009083763A1 (en) 2009-07-09
EP2225393B1 (en) 2015-02-25

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