ES2537625T3 - Método para hibridar ácidos nucleicos - Google Patents

Método para hibridar ácidos nucleicos Download PDF

Info

Publication number
ES2537625T3
ES2537625T3 ES08868164.8T ES08868164T ES2537625T3 ES 2537625 T3 ES2537625 T3 ES 2537625T3 ES 08868164 T ES08868164 T ES 08868164T ES 2537625 T3 ES2537625 T3 ES 2537625T3
Authority
ES
Spain
Prior art keywords
primers
conjugate
different
oligonucleotide
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08868164.8T
Other languages
English (en)
Spanish (es)
Inventor
Nathalie Lenne
Patrick Erbacher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite de Strasbourg
Polyplus Transfection SA
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite de Strasbourg
Polyplus Transfection SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Universite de Strasbourg, Polyplus Transfection SA filed Critical Centre National de la Recherche Scientifique CNRS
Application granted granted Critical
Publication of ES2537625T3 publication Critical patent/ES2537625T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES08868164.8T 2007-12-27 2008-09-12 Método para hibridar ácidos nucleicos Active ES2537625T3 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US914507P 2007-12-27 2007-12-27
EP07025148A EP2075342A1 (en) 2007-12-27 2007-12-27 Method for hybridizing nucleic acids
EP07025148 2007-12-27
US9145P 2007-12-27
PCT/IB2008/002789 WO2009083763A1 (en) 2007-12-27 2008-09-12 Method for hybridizing nucleic acids

Publications (1)

Publication Number Publication Date
ES2537625T3 true ES2537625T3 (es) 2015-06-10

Family

ID=39339663

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08868164.8T Active ES2537625T3 (es) 2007-12-27 2008-09-12 Método para hibridar ácidos nucleicos

Country Status (14)

Country Link
US (1) US8465920B2 (enExample)
EP (2) EP2075342A1 (enExample)
JP (1) JP2011507542A (enExample)
KR (1) KR20100124705A (enExample)
CN (1) CN101978071A (enExample)
AU (1) AU2008345460A1 (enExample)
BR (1) BRPI0821698A2 (enExample)
CA (1) CA2709183A1 (enExample)
DK (1) DK2225393T3 (enExample)
ES (1) ES2537625T3 (enExample)
IL (1) IL206542A0 (enExample)
NZ (1) NZ586373A (enExample)
RU (1) RU2010131166A (enExample)
WO (1) WO2009083763A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011108930A1 (en) 2010-03-04 2011-09-09 Interna Technologies Bv A MiRNA MOLECULE DEFINED BY ITS SOURCE AND ITS DIAGNOSTIC AND THERAPEUTIC USES IN DISEASES OR CONDITIONS ASSOCIATED WITH EMT
EP2591106A1 (en) 2010-07-06 2013-05-15 InteRNA Technologies B.V. Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway
CN103080310A (zh) * 2010-09-10 2013-05-01 三菱化学美迪恩斯株式会社 使用光的核酸的扩增抑制方法和高灵敏度的选择性核酸扩增方法
EP2474617A1 (en) 2011-01-11 2012-07-11 InteRNA Technologies BV Mir for treating neo-angiogenesis
WO2013095132A1 (en) 2011-12-22 2013-06-27 Interna Technologies B.V. Mirna for treating head and neck cancer
KR102146523B1 (ko) * 2012-01-06 2020-08-20 조원창 표적 핵산의 향상된 증폭
WO2014072357A1 (en) 2012-11-06 2014-05-15 Interna Technologies B.V. Combination for use in treating diseases or conditions associated with melanoma, or treating diseases or conditions associated with activated b-raf pathway
US9410172B2 (en) * 2013-09-16 2016-08-09 General Electric Company Isothermal amplification using oligocation-conjugated primer sequences
DE102017123919A1 (de) 2017-10-13 2019-04-18 Gna Biosolutions Gmbh Verfahren und Vorrichtung zur Lyse von Mikroorganismen
CN111566212A (zh) 2017-11-03 2020-08-21 因特尔纳技术有限公司 miRNA分子，等同物，安塔够妙或其来源用于治疗和/或诊断与神经元缺陷相关的病症和/或疾病或用于神经元生成和/或再生
JP2021513508A (ja) 2018-02-12 2021-05-27 インテアールエヌエー テクノロジーズ ビー．ヴイ．ＩｎｔｅＲＮＡ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｂ．Ｖ． 抗がんマイクロｒｎａ及びその脂質製剤
KR20200045212A (ko) 2018-10-22 2020-05-04 (주)바이오니아 옥타민 또는 옥타민 유도체가 결합된 프로브 및 이의 용도
CN113444778A (zh) 2020-03-27 2021-09-28 广州达安基因股份有限公司 具有高杂交性能的寡核苷酸缀合物及其应用
IT202200026595A1 (it) 2022-12-22 2024-06-22 Fond Telethon Ets Nuovi inibitori di regolatori epigenetici

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1013770A1 (en) * 1998-12-23 2000-06-28 Université Louis Pasteur de Strasbourg Non-viral transfection vector
CA2581869A1 (en) * 2004-11-04 2006-05-18 Raul Andino Syntheses of polyamine conjugates of small interfering rnas (si-rnas) and conjugates formed thereby
BRPI0619932A2 (pt) 2005-12-15 2011-10-25 Centre Nat Rech Scient moléculas de oligonucleotìdeo-oligocátion, método para a obtenção das mesmas, reagentes de fosforamidita, método para uso em biologia e diagnóstico, e, composições farmacêuticas

Also Published As

Publication number Publication date
IL206542A0 (en) 2010-12-30
AU2008345460A1 (en) 2009-07-09
US8465920B2 (en) 2013-06-18
CA2709183A1 (en) 2009-07-09
CN101978071A (zh) 2011-02-16
RU2010131166A (ru) 2012-02-10
BRPI0821698A2 (pt) 2015-06-16
EP2075342A1 (en) 2009-07-01
WO2009083763A1 (en) 2009-07-09
JP2011507542A (ja) 2011-03-10
EP2225393A1 (en) 2010-09-08
EP2225393B1 (en) 2015-02-25
NZ586373A (en) 2012-09-28
DK2225393T3 (en) 2015-05-26
US20100311056A1 (en) 2010-12-09
KR20100124705A (ko) 2010-11-29

Similar Documents

Publication Publication Date Title
ES2537625T3 (es) Método para hibridar ácidos nucleicos
JP4644370B2 (ja) 標的ハイブリダイゼーション検出のための、プローブおよびクランプのバイナリー組成物ならびに方法
AU2007306019B2 (en) Double-stranded probes for the fluorescent detection of nucleic acids
US20040229253A1 (en) Compositions and methods for multiplex analysis of polynucleotides
US20220090195A1 (en) Structure and application of double-stranded oligonucleotide nucleic acid probe
HUE032212T2 (en) Polymer chain reaction detection system using oligonucleotides containing phosphorothioate group
US20160010152A1 (en) Dual Probe:Antiprobe Compositions for DNA and RNA Detection
ES2776427T3 (es) Método de amplificación de ADN basado en invasión de cadena
ES2925313T3 (es) Método para la detección de secuencias de ácido nucleico diana
CN100577816C (zh) 一种荧光标记的寡核苷酸探针及其应用
US20080051295A1 (en) Method Of Detecting Nucleic Acid Using Amplification On An Array
JPWO2012036111A1 (ja) 多検体解析のためのプライマー、プローブおよび方法
CN101796185B (zh) 扩增甲基化核酸或非甲基化核酸的方法
JP5597939B2 (ja) 部分競合型プローブを用いた標的塩基配列の検出方法
ES2644839T3 (es) Procedimiento, composiciones y kits para determinar el virus de la inmunodeficiencia humana (VIH)
WO2023116408A1 (zh) 一种核酸探针及其使用方法
CN119876355B (zh) 用于实时荧光定量pcr的组合物及其应用
US12331355B2 (en) Oligonucleotide conjugate with high hybridization performance and use thereof
US20110136105A1 (en) Methods of quantifying nucleic acids
ES2946232A1 (es) SISTEMA DE DETECCION DE VARIANTES DE SARS-CoV-2 MEDIANTE RT-qPCR
AU4283696A (en) Self-quenching fluorescence probe and method