JP2009517013A5 - - Google Patents

Info

Publication number

JP2009517013A5

JP2009517013A5 JP2008541798A JP2008541798A JP2009517013A5 JP 2009517013 A5 JP2009517013 A5 JP 2009517013A5 JP 2008541798 A JP2008541798 A JP 2008541798A JP 2008541798 A JP2008541798 A JP 2008541798A JP 2009517013 A5 JP2009517013 A5 JP 2009517013A5

Authority

JP

Japan

Prior art keywords

oligonucleotide

seq

gene

sequence

amplification

Prior art date

2006-11-23

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

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• 108091034117 Oligonucleotide Proteins 0.000 claims description 45
• 230000003321 amplification Effects 0.000 claims description 30
• 238000003199 nucleic acid amplification method Methods 0.000 claims description 30
• 239000000523 sample Substances 0.000 claims description 26
• 230000000295 complement effect Effects 0.000 claims description 22
• 238000001514 detection method Methods 0.000 claims description 12
• 239000003068 molecular probe Substances 0.000 claims description 5
• 239000002773 nucleotide Substances 0.000 claims 26
• 125000003729 nucleotide group Chemical group 0.000 claims 26
• 238000000034 method Methods 0.000 claims 18
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 16
• 241000712431 Influenza A virus Species 0.000 claims 13
• 101150105849 H5 gene Proteins 0.000 claims 12
• 108020004707 nucleic acids Proteins 0.000 claims 11
• 150000007523 nucleic acids Chemical class 0.000 claims 11
• 102000039446 nucleic acids Human genes 0.000 claims 11
• 101150098863 N1 gene Proteins 0.000 claims 10
• 238000006243 chemical reaction Methods 0.000 claims 9
• 239000003153 chemical reaction reagent Substances 0.000 claims 8
• 108090000623 proteins and genes Proteins 0.000 claims 5
• 108020004414 DNA Proteins 0.000 claims 4
• 102000004190 Enzymes Human genes 0.000 claims 4
• 108090000790 Enzymes Proteins 0.000 claims 4
• 230000001419 dependent effect Effects 0.000 claims 4
• 230000000694 effects Effects 0.000 claims 4
• 238000002372 labelling Methods 0.000 claims 4
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 3
• 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 3
• 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 3
• BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 claims 2
• 108091093088 Amplicon Proteins 0.000 claims 1
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
• 102100034343 Integrase Human genes 0.000 claims 1
• 101710203526 Integrase Proteins 0.000 claims 1
• 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
• 239000012472 biological sample Substances 0.000 claims 1
• 239000011541 reaction mixture Substances 0.000 claims 1
• 238000003757 reverse transcription PCR Methods 0.000 claims 1
• 230000002103 transcriptional effect Effects 0.000 claims 1
• 238000009396 hybridization Methods 0.000 description 6
• OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
• 239000006096 absorbing agent Substances 0.000 description 1
• 230000001939 inductive effect Effects 0.000 description 1
• 239000000126 substance Substances 0.000 description 1