CN101952439A - 无抗生素状态下产生多肽的表达系统 - Google Patents

无抗生素状态下产生多肽的表达系统 Download PDF

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Publication number
CN101952439A
CN101952439A CN2008800147494A CN200880014749A CN101952439A CN 101952439 A CN101952439 A CN 101952439A CN 2008800147494 A CN2008800147494 A CN 2008800147494A CN 200880014749 A CN200880014749 A CN 200880014749A CN 101952439 A CN101952439 A CN 101952439A
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China
Prior art keywords
dna
sequence
glycerol
host cell
polypeptide
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Pending
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CN2008800147494A
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English (en)
Chinese (zh)
Inventor
斯爱琳·卡多特
啼娜·普劳斯
璐斯·史维尔德福哲
布如诺·文因特
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AB Enzymes GmbH
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AB Enzymes GmbH
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Application filed by AB Enzymes GmbH filed Critical AB Enzymes GmbH
Publication of CN101952439A publication Critical patent/CN101952439A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN2008800147494A 2007-05-04 2008-03-12 无抗生素状态下产生多肽的表达系统 Pending CN101952439A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102007021001.0 2007-05-04
DE102007021001A DE102007021001A1 (de) 2007-05-04 2007-05-04 Expressionssystem zur antibiotikafreien Produktion von Polypeptiden
PCT/EP2008/001977 WO2008135113A1 (de) 2007-05-04 2008-03-12 Expressionssystem zur antibiotikafreien produktion von polypeptiden

Publications (1)

Publication Number Publication Date
CN101952439A true CN101952439A (zh) 2011-01-19

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ID=39809652

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2008800147494A Pending CN101952439A (zh) 2007-05-04 2008-03-12 无抗生素状态下产生多肽的表达系统

Country Status (12)

Country Link
US (1) US8465946B2 (de)
EP (1) EP2145006B1 (de)
CN (1) CN101952439A (de)
AT (1) ATE509109T1 (de)
AU (1) AU2008249045B2 (de)
BR (1) BRPI0811501B1 (de)
CA (1) CA2686263C (de)
DE (1) DE102007021001A1 (de)
DK (1) DK2145006T3 (de)
MX (1) MX2009011934A (de)
WO (1) WO2008135113A1 (de)
ZA (1) ZA200907647B (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117487836A (zh) * 2023-12-29 2024-02-02 苏州优信合生技术有限公司 一种大肠杆菌无抗生素表达系统及其在高效表达目的基因中的应用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105849267A (zh) * 2013-12-20 2016-08-10 巴斯夫欧洲公司 在宿主微生物中产生目的蛋白质的方法
ES2855992T3 (es) 2015-12-11 2021-09-27 Wacker Chemie Ag Cepa de microorganismos y procedimiento para la producción fermentativa exenta de antibióticos de sustancias y proteínas de bajo peso molecular
EP3926039A1 (de) 2020-06-17 2021-12-22 AB Enzymes GmbH Verwendung einer nuklease zur verringerung der viskosität und/oder zur verhinderung eines viskositätsanstiegs einer fermentationsbrühe
BR112023027016A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus compreendendo uma proteína reguladora da matriz extracelular a alterada e/ou uma proteína reguladora da matriz extracelular b alterada, método para produzir um composto de interesse, e, proteínas reguladoras das matrizes extracelulares a e b alteradas
CA3234382A1 (en) 2021-11-09 2023-05-19 Tina Ploss Improved xylanases

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK594084A (da) 1984-12-12 1986-06-13 Novo Industri As Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
EP0251579A3 (de) 1986-06-24 1989-03-22 Enterovax Research Pty. Ltd. Markiersystem ohne Antibiotika
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
JP2637532B2 (ja) 1987-02-27 1997-08-06 ギスト ブロカデス ナームローゼ フェンノートチャップ 原核微生物の染色体ｄｎａにおける安定した遺伝子増幅
DD277467A1 (de) 1988-12-01 1990-04-04 Akad Wissenschaften Ddr Verfahren zur herstellung von extrazellulaeren enzymen, insbesondere von neutraler protease
DE4226528A1 (de) 1992-08-11 1994-02-17 Roehm Gmbh Batterien-Xylanase, Verfahren zu ihrer Herstellung sowie dafür geeigneter Bakterienstamm, Plasmid mit zugehörigem Strukturgen, sowie Backmittel und Backverfahren zur Herstellung von Brot und Backwaren unter Verwendung der Xylanase
DE69631118T2 (de) 1995-01-23 2004-07-01 Novozymes A/S Dna-integration durch transposition
US6762040B2 (en) 2000-05-24 2004-07-13 Novozymes A/S Method for increasing gene copy number in a host cell and resulting host cell
DE10309557A1 (de) 2003-03-04 2004-09-23 Henkel Kgaa Ein Translokationsenzym als Selektionsmarker
DE10360483B4 (de) 2003-12-22 2007-11-15 Scil Proteins Gmbh Expressionsvektor und dessen Verwendung

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117487836A (zh) * 2023-12-29 2024-02-02 苏州优信合生技术有限公司 一种大肠杆菌无抗生素表达系统及其在高效表达目的基因中的应用
CN117487836B (zh) * 2023-12-29 2024-04-19 苏州优信合生技术有限公司 一种大肠杆菌无抗生素表达系统及其在高效表达目的基因中的应用

Also Published As

Publication number Publication date
ATE509109T1 (de) 2011-05-15
WO2008135113A4 (de) 2009-03-12
WO2008135113A8 (de) 2009-01-15
EP2145006B1 (de) 2011-05-11
DE102007021001A1 (de) 2008-11-06
CA2686263A1 (en) 2008-11-13
BRPI0811501A2 (pt) 2021-06-01
MX2009011934A (es) 2009-12-08
US8465946B2 (en) 2013-06-18
ZA200907647B (en) 2011-02-23
DK2145006T3 (da) 2011-07-18
BRPI0811501B1 (pt) 2022-03-29
US20100248306A1 (en) 2010-09-30
EP2145006A1 (de) 2010-01-20
WO2008135113A1 (de) 2008-11-13
AU2008249045A1 (en) 2008-11-13
AU2008249045B2 (en) 2013-05-16
CA2686263C (en) 2014-04-29

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Application publication date: 20110119