DK2145006T3 - Ekspressionssystem anvendt til antibiotikafri fremstilling af polypeptider - Google Patents

Ekspressionssystem anvendt til antibiotikafri fremstilling af polypeptider

Info

Publication number
DK2145006T3
DK2145006T3 DK08734603.7T DK08734603T DK2145006T3 DK 2145006 T3 DK2145006 T3 DK 2145006T3 DK 08734603 T DK08734603 T DK 08734603T DK 2145006 T3 DK2145006 T3 DK 2145006T3
Authority
DK
Denmark
Prior art keywords
expression system
antibiotic
glycerine
polypeptides
phosphate dehydrogenase
Prior art date
Application number
DK08734603.7T
Other languages
English (en)
Inventor
Celine Cadot
Ruth Schwerdtfeger
Bruno Winter
Tina Ploss
Original Assignee
Ab Enzymes Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ab Enzymes Gmbh filed Critical Ab Enzymes Gmbh
Application granted granted Critical
Publication of DK2145006T3 publication Critical patent/DK2145006T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK08734603.7T 2007-05-04 2008-03-12 Ekspressionssystem anvendt til antibiotikafri fremstilling af polypeptider DK2145006T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102007021001A DE102007021001A1 (de) 2007-05-04 2007-05-04 Expressionssystem zur antibiotikafreien Produktion von Polypeptiden
PCT/EP2008/001977 WO2008135113A1 (de) 2007-05-04 2008-03-12 Expressionssystem zur antibiotikafreien produktion von polypeptiden

Publications (1)

Publication Number Publication Date
DK2145006T3 true DK2145006T3 (da) 2011-07-18

Family

ID=39809652

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08734603.7T DK2145006T3 (da) 2007-05-04 2008-03-12 Ekspressionssystem anvendt til antibiotikafri fremstilling af polypeptider

Country Status (12)

Country Link
US (1) US8465946B2 (da)
EP (1) EP2145006B1 (da)
CN (1) CN101952439A (da)
AT (1) ATE509109T1 (da)
AU (1) AU2008249045B2 (da)
BR (1) BRPI0811501B1 (da)
CA (1) CA2686263C (da)
DE (1) DE102007021001A1 (da)
DK (1) DK2145006T3 (da)
MX (1) MX2009011934A (da)
WO (1) WO2008135113A1 (da)
ZA (1) ZA200907647B (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015091280A1 (en) * 2013-12-20 2015-06-25 Basf Se A process for production of a protein of interest in a microbial host organism
DK3556847T3 (da) 2015-12-11 2021-05-25 Wacker Chemie Ag Mikroorganismestamme og fremgangsmåde til antibiotikafri, fermentativ fremstilling af lavmolekylære substanser og proteiner
EP3926039A1 (en) 2020-06-17 2021-12-22 AB Enzymes GmbH Use of a nuclease for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth
MX2023015460A (es) 2021-06-24 2024-01-18 Basf Se Celula huesped de bacillus mejorada con proteina rema/remb alterada.
CN118202042A (zh) 2021-11-09 2024-06-14 Ab酶有限公司 改进的木聚糖酶
CN117487836B (zh) * 2023-12-29 2024-04-19 苏州优信合生技术有限公司 一种大肠杆菌无抗生素表达系统及其在高效表达目的基因中的应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK594084A (da) 1984-12-12 1986-06-13 Novo Industri As Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
EP0251579A3 (en) 1986-06-24 1989-03-22 Enterovax Research Pty. Ltd. Non-antibiotic marker system
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
RU2091487C1 (ru) 1987-02-27 1997-09-27 Гист-Брокейдс Н.В. Способ получения штамма bacillus, содержащего две копии последовательности днк, кодирующей фермент с гидролазной активностью
DD277467A1 (de) 1988-12-01 1990-04-04 Akad Wissenschaften Ddr Verfahren zur herstellung von extrazellulaeren enzymen, insbesondere von neutraler protease
DE4226528A1 (de) 1992-08-11 1994-02-17 Roehm Gmbh Batterien-Xylanase, Verfahren zu ihrer Herstellung sowie dafür geeigneter Bakterienstamm, Plasmid mit zugehörigem Strukturgen, sowie Backmittel und Backverfahren zur Herstellung von Brot und Backwaren unter Verwendung der Xylanase
DE69631118T2 (de) 1995-01-23 2004-07-01 Novozymes A/S Dna-integration durch transposition
WO2001090393A1 (en) 2000-05-24 2001-11-29 Novozymes A/S Method for increasing gene copy number in a host cell and resulting host cell
DE10309557A1 (de) 2003-03-04 2004-09-23 Henkel Kgaa Ein Translokationsenzym als Selektionsmarker
DE10360483B4 (de) 2003-12-22 2007-11-15 Scil Proteins Gmbh Expressionsvektor und dessen Verwendung

Also Published As

Publication number Publication date
EP2145006B1 (de) 2011-05-11
ZA200907647B (en) 2011-02-23
BRPI0811501B1 (pt) 2022-03-29
MX2009011934A (es) 2009-12-08
AU2008249045A1 (en) 2008-11-13
US20100248306A1 (en) 2010-09-30
US8465946B2 (en) 2013-06-18
ATE509109T1 (de) 2011-05-15
CA2686263A1 (en) 2008-11-13
DE102007021001A1 (de) 2008-11-06
BRPI0811501A2 (pt) 2021-06-01
WO2008135113A1 (de) 2008-11-13
WO2008135113A8 (de) 2009-01-15
CN101952439A (zh) 2011-01-19
AU2008249045B2 (en) 2013-05-16
EP2145006A1 (de) 2010-01-20
WO2008135113A4 (de) 2009-03-12
CA2686263C (en) 2014-04-29

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