CN101946631A - Bud pressing method for grifola frondosa - Google Patents
Bud pressing method for grifola frondosa Download PDFInfo
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- CN101946631A CN101946631A CN 201010251549 CN201010251549A CN101946631A CN 101946631 A CN101946631 A CN 101946631A CN 201010251549 CN201010251549 CN 201010251549 CN 201010251549 A CN201010251549 A CN 201010251549A CN 101946631 A CN101946631 A CN 101946631A
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- mycelia
- flower bud
- scarfing
- bacterium bag
- original hase
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Abstract
The invention provides a bud pressing method for grifola frondosa. The method comprises the following steps of: inoculation and culture of hyphae, cutting, recovery of hyphae, primary bud pressing, secondary bud pressing and growth period. The method can obviously raise the ratio of forming anlages of the grifola frondosa, and improve the quality of the formed anlages.
Description
Affiliated technical field:
The present invention relates to field of edible fungus culture, relate in particular to a kind of promote grifola frondosus pocket type annually cultivating original hase to form and differentiation urge the flower bud method.
Background technology:
Grifola frondosus has peculiar fragrance and mouthfeel, more receive much concern because of its outstanding anticancer health-care function, utilize grifola frondosus to make the health products of high-quality (patent No. 200410083062) at present, its edible medicinal is worth the recognition and acceptance that is subjected to people day by day, has vast market prospect future.At present, China only Qingyuan County, Zhejiang Province and Qianxi County, Hebei province utilizes natural conditions to set up the production base of scale, and the scientific research institutions of various places carry out small-scale experimental planting test.Experiment in cultivation shows grifola frondosus original hase formation rate less than 80%, and original hase forms obstacle and become the bottleneck that the grifola frondosus artificial cultivation must be broken through.
Summary of the invention:
Technical problem to be solved by this invention is to provide a kind of flower bud method of urging of grifola frondosus to form problem on obstacle with solution present stage original hase, improves the tame output of grifola frondosus, and assurance grifola frondosus anniversary bag is planted and achieved success.
Grifola frondosus anniversary bag of the present invention is planted two-period form and is urged flower bud technology to comprise following step:
A) mycelium inoculation and cultivation: mycelium inoculation is in the bacterium bag, and then the bacterium bag being placed temperature is that 22～24 ℃, relative moisture are 60%, CO
2Concentration is that cultivate in the darkroom of 1000～2000ppm; Behind the long saturating bacterium bag of mycelia again after-ripening 20～25d be beneficial to the mycelia maturation;
This stage should guarantee abundant after-ripening of mycelia and cell age unanimity, urges the flower bud fruiting conforming to guarantee the later stage, and will make regular check on the bacterium bag of choosing microbial contamination between culture period.
B) scarfing: the bacterium bag of physiological ripening is shifted out culturing room, select the dense white place of mycelia, bacterium bag surface carries out disinfection, and cuts the mouth of diameter 1～1.5cm, degree of depth 1cm then with the blade of sterilizing, and cuts out mycelium;
Must equating scarfing surface, remove unnecessary mycelium behind the scarfing, in order to avoid the bacterium and the mould of the excessive generation contaminative of later stage cultivation humidity.
C) mycelia recovers: mycelia begins to recover behind the scarfing, and mycelia progressively covers scarfing, and mycelia clenches and make progress protuberance; The condition that controls environment temperature: 23～25 ℃, humidity: 96～99%;
This, mycelia clenched and protuberance upwards in stage, was the critical period that original hase forms, and will guarantee the every day three times of water spray earthward this moment, and lucifuge is handled, and ventilates and interior circulation minimizing as far as possible, is beneficial to the fast quick-recovery of mycelia.
D) once urge flower bud: the mycelia kink after the recovery forms the small embossment of diameter a 3～5mm gradually, and the formation grifola frondosus original hase that constantly upwards grows; Keeping temperature behind the mycelia recovery covering scarfing charge level is 22～24 ℃, and humidity keeps 98～100%, ventilation, lucifuge processing;
Need not give illumination this period, because the very fast differentiation of original hase has a strong impact on later stage output under illumination condition.The scarfing place forms a lot of little original hases, is fused to together gradually after the growth.Treat that the original hase diameter reaches about 3cm, once urge the flower bud stage to finish, enter the fertility chamber and carry out two sections and urge flower bud to handle.
E) secondary is urged flower bud: will once urge flower bud bacterium bag later to choose carrying out two sections into the fertility chamber and urge flower bud to handle; Reduce temperature to 19～22 ℃, humidity 95～99% increases illumination; The alveole edge for the treatment of the former primary surface of grifola frondosus grows linen projection gradually, and differentiates stigma one by one rapidly, and whole original hase urges flower bud to finish for two sections as coral at this moment;
Original hase is very fast under the stimulation of the temperature difference and illumination becomes grey black by white, and along with the growth change of inner cell, original hase is grown rapidly and become big, and the depression that suffers setback on the surface presents the form of brain shape body; Secrete flaxen liquid, toughness in the punishment of brain shape body depression alveole subsequently; Must keep the weak yellow liquid in the nest to disperse naturally, forbid that artificial way removes otherwise cause the original hase maldevelopment by it.The stigma of former primary surface of coral phase is the basis that Grifola Frondosa sporophore " blade " forms, and the developmental state of stigma directly causes the quality of Grifola Frondosa sporophore.
F) vegetative period: this stage keeps 19～21 ℃ of temperature, and humidity 90～98% reduces space CO
2Concentration is below 1000ppm.
Accelerate because of the growing of original hase, cell metabolism this period, and the humidity in space is difficult to satisfy the growth needs of original hase.The micro-spray system humidification in using industrialized cultivation house, can use agroatomizer directly to spray water at this moment to the grifola frondosus original hase.But note to make a large amount of waters on the original hase, keep moistening getting final product.Original hase is divided into fan-shaped blade gradually, and color shoals and is canescence.When treating that fine aperture appears in the grifola frondosus vacuum side of blade, maturation is described, but timely collecting.
Adopt two-period form of the present invention to urge flower bud technology can significantly improve the grifola frondosus original hase and form ratio, the original hase formation rate significantly improves, and reaches more than 95%.And the original hase formation time concentrates on scarfing and handles back 15d～25d, is convenient to the later stage unified management.This process has effectively improved original hase and has formed product, quality, and biology efficient reaches 40%, the sporophore shape grace.This technology is that grifola frondosus high yield, high-quality production are taken a firm foundation.
Embodiment
Grifola frondosus anniversary bag provided by the invention is planted two-period form and is urged flower bud technology concrete steps as follows:
1, mycelium inoculation and cultivation: postvaccinal Grifolas frondosa germ bag is placed 22～24 ℃ of temperature, relative moisture 60%, CO
2Concentration 1000～2000ppm, cultivate in the darkroom.Cultivate 10d, bacterial classification is sprouted and is covered bacterium bag upper limb, and whether can check for the first time has living contaminants.Cultivate 22d, mycelia long to the bacterium bag half, can check the bacterium of mixing for the 2nd time.Cultivate 40d, the long saturating bacterium bag of mycelia can carry out the 3rd assorted bacterium and check.Need after-ripening 20～25d to be beneficial to the mycelia maturation behind the long saturating bacterium bag of mycelia.Cultivation stage should guarantee abundant after-ripening of mycelia and cell age unanimity, urges flower bud fruiting uniformity to guarantee the later stage.
2, scarfing: the bacterium bag of physiological ripening is shifted out culturing room carry out scarfing and handle.Select the dense white place of mycelia,, afterwards cut the circular port of diameter 1～1.5cm, degree of depth 1cm, cut out mycelium with the blade of sterilizing earlier with 75% alcohol wipe bacterium bag surface sterilization.Must equating scarfing surface, remove unnecessary mycelium behind the scarfing, in order to avoid the bacterium and the mould of the excessive generation contaminative of later stage cultivation humidity.Bacterium bag scarfing position and scarfing blade must cause grifola frondosus product, quality to descend otherwise urge Lei Shiyi to produce yellow water through 75% alcohol wipe surface sterilization.
3, mycelia recovers: the bacterium bag behind the scarfing should change cultivating chamber at once over to, and overlong time scarfing surface moisture scatters and disappears influences original hase formation.Progressively scarfing is covered after mycelia recovers, and the mycelia around the scarfing is also obviously in vain in other places.This, mycelia clenched and protuberance upwards in period, was the critical period that original hase forms.The temperature control that focuses on of these stage environment condition regulation and control is preserved moisture.The environmental condition temperature is set: 23～25 ℃, humidity: 96～99%, ventilate and interior circulation minimizing as far as possible.Every day three times is water spray earthward, and lucifuge is handled, and is beneficial to the fast quick-recovery of mycelia.
4, once urge flower bud: mycelium is twisted together mutually upwards to grow and is formed the grifola frondosus original hase.Mycelia recover to cover and to reduce temperature to 22～24 ℃ behind the scarfing charge level, and humidity keeps 98～100%, suitably increases ventilation.Need not give illumination this period, because the very fast differentiation of original hase has a strong impact on later stage output under illumination condition.The scarfing place forms a lot of little original hases, is fused to together gradually after the growth.Treat that the original hase diameter reaches about 3cm, once urge the flower bud stage to finish, enter the fertility chamber and carry out two sections and urge flower bud to handle.
5, secondary is urged flower bud: will once urge flower bud bacterium bag later to choose carrying out two sections into the fertility chamber and urge flower bud to handle.Reduce temperature to 19～22 ℃, humidity 95～99% increases illumination.Original hase is very fast under the stimulation of the temperature difference and illumination becomes grey black by white.Along with the growth change of inner cell, original hase is grown rapidly and is become big, and the depression that suffers setback on the surface presents the form of brain shape body.Secrete flaxen liquid, toughness in the punishment of brain shape body depression alveole subsequently.Must keep the weak yellow liquid in the nest to disperse naturally, forbid that artificial way removes otherwise cause the original hase maldevelopment by it.Alveole edge at the former primary surface of grifola frondosus grows linen projection gradually, and differentiates stigma one by one rapidly, and whole original hase urges flower bud to finish for two sections as coral at this moment.The stigma of former primary surface of coral phase is the basis that Grifola Frondosa sporophore " blade " forms, and the developmental state of stigma directly causes the quality of Grifola Frondosa sporophore.
6, vegetative period: this stage should keep 19～21 ℃ of temperature, reduces space CO as far as possible
2Below the concentration 1000ppm.Accelerate because of the growing of original hase, cell metabolism this period, and the humidity in space is difficult to satisfy the growth needs of original hase.The micro-spray system humidification in using industrialized cultivation house, can use agroatomizer directly to spray water at this moment to the grifola frondosus original hase.But note to make a large amount of waters on the original hase, keep moistening getting final product.Original hase is divided into fan-shaped blade gradually, and color shoals and is canescence.When treating that fine aperture appears in the grifola frondosus vacuum side of blade, maturation is described, but timely collecting.
Embodiment one:
With the Grifolas frondosa germ strain No.1 (preservation of DSMZ of Edible Fungus Inst., Shanghai Academy of Agriculture, preserving number: 2597) be example:
Conventional method is carried out mycelium inoculation, in the cultural hypha stage Grifolas frondosa germ bag is placed 22 ℃ of temperature, relative moisture 60%, CO then
2Concentration 1000～2000ppm, cultivate in the darkroom.Cultivate 10d, 22d, 40d carry out the living contaminants inspection respectively.Need after-ripening 20d to be beneficial to the mycelia maturation behind the long saturating bacterium bag of mycelia.The bacterium bag of physiological ripening is shifted out culturing room to carry out scarfing and handles.Select the dense white place of mycelia,, afterwards cut the circular port of diameter 1～1.5cm, degree of depth 1cm, cut out mycelium with the blade of sterilizing earlier with 75% alcohol wipe bacterium bag surface sterilization.Progressively scarfing is covered after mycelia recovers, and the mycelia around the scarfing is also obviously in vain in other places.The temperature control that focuses on of these stage environment condition regulation and control is preserved moisture.The environmental condition temperature is set: 23 ℃, humidity: 96～99%, ventilate and interior circulation minimizing as far as possible.Every day three times is water spray earthward, and lucifuge is handled, and is beneficial to the fast quick-recovery of mycelia.Mycelia recover to cover and to reduce temperature to 22 ℃ behind the scarfing charge level, and humidity keeps suitably increasing ventilation more than 98%.Need not give illumination this period.The scarfing place forms a lot of little original hases, is fused to together gradually after the growth.Treat that the original hase diameter reaches about 3cm, once urge the flower bud stage to finish.After enter the fertility chamber and carry out two sections and urge flower bud to handle: reduce temperature to 21 ℃, humidity 95～99% increases illumination.Original hase is very fast under the stimulation of the temperature difference and illumination becomes grey black by white, and back original hase differentiation presents the form of brain shape body, and brain shape body alveole edge grows linen projection gradually, and differentiates stigma one by one rapidly, urges flower bud to finish for two sections at this moment.Stigma is the basis that Grifola Frondosa sporophore " blade " forms, and the developmental state of stigma directly causes the quality of Grifola Frondosa sporophore.Stage No. 1 strain growth phase of grifola frondosus should keep 19 ℃ of temperature, reduces space CO as far as possible
2Below the concentration 1000ppm.The micro-spray system spraying of this moment in using industrialized cultivation house, can use agroatomizer directly to spray water to the grifola frondosus original hase.When treating that fine aperture appears in the grifola frondosus vacuum side of blade, maturation is described, but timely collecting.
Embodiment two:
With No. 2, Grifola frondosa strain (be the preservation of DSMZ of Edible Fungus Inst., Shanghai Academy of Agriculture, preserving number: 2584) be example:
The Grifolas frondosa germ bag is placed 22 ℃ of temperature, relative moisture 60%, CO
2Concentration 1000～2000ppm, cultivate in the darkroom.Cultivate 10d, 22d, 40d carry out the living contaminants inspection respectively.Need after-ripening 25d to be beneficial to the mycelia maturation behind the long saturating bacterium bag of mycelia.The bacterium bag of physiological ripening is shifted out culturing room to carry out scarfing and handles.Select the dense white place of mycelia,, afterwards cut the circular port of diameter 1～1.5cm, degree of depth 1cm, cut out mycelium with the blade of sterilizing earlier with 75% alcohol wipe bacterium bag surface sterilization.Progressively scarfing is covered after mycelia recovers, and the mycelia around the scarfing is also obviously in vain in other places.The temperature control that focuses on of these stage environment condition regulation and control is preserved moisture.The environmental condition temperature is set: 24 ℃, humidity: 96～99%, ventilate and interior circulation minimizing as far as possible.Every day three times is water spray earthward, and lucifuge is handled, and is beneficial to the fast quick-recovery of mycelia.Mycelia recover to cover and to reduce temperature to 23 ℃ behind the scarfing charge level, and humidity keeps suitably increasing ventilation more than 98%.Need not give illumination this period.The scarfing place forms a lot of little original hases, is fused to together gradually after the growth.Treat that the original hase diameter reaches about 3cm, once urge the flower bud stage to finish.After enter the fertility chamber and carry out two sections and urge flower bud to handle: reduce temperature to 22 ℃, humidity 95～99% increases illumination.Original hase is very fast under the stimulation of the temperature difference and illumination becomes grey black by white, and back original hase differentiation presents the form of brain shape body, and brain shape body alveole edge grows linen projection gradually, and differentiates stigma one by one rapidly, urges flower bud to finish for two sections at this moment.Stigma is the basis that Grifola Frondosa sporophore " blade " forms, and the developmental state of stigma directly causes the quality of Grifola Frondosa sporophore.Stage No. 2 strain growth phases of grifola frondosus should keep 20 ℃ of temperature, reduces space CO as far as possible
2Below the concentration 1000ppm.The micro-spray system spraying of this moment in using industrialized cultivation house, can use agroatomizer directly to spray water to the grifola frondosus original hase.When treating that fine aperture appears in the grifola frondosus vacuum side of blade, maturation is described, but timely collecting.
Claims (1)
- A grifola frondosus urge the flower bud method, it is characterized in that comprising following step:A) mycelium inoculation and cultivation: mycelium inoculation is in the bacterium bag, and then the bacterium bag being placed temperature is that 22～24 ℃, relative moisture are 60%, CO 2Concentration is that cultivate in the darkroom of 1000～2000ppm; Behind the long saturating bacterium bag of mycelia again after-ripening 20～25d be beneficial to the mycelia maturation;B) scarfing: the bacterium bag of physiological ripening is shifted out culturing room, select the dense white place of mycelia, bacterium bag surface carries out disinfection, and cuts the mouth of diameter 1～1.5cm, degree of depth 1cm then with the blade of sterilizing, and cuts out mycelium;C) mycelia recovers: mycelia begins to recover behind the scarfing, and mycelia progressively covers scarfing, and mycelia clenches and make progress protuberance; The condition that controls environment temperature: 23～25 ℃, humidity: 96～99%;D) once urge flower bud: the mycelia kink after the recovery forms the small embossment of diameter a 3～5mm gradually, and the formation grifola frondosus original hase that constantly upwards grows; Keeping temperature behind the mycelia recovery covering scarfing charge level is 22～24 ℃, and humidity keeps 98～100%, ventilation, lucifuge processing;E) secondary is urged flower bud: will once urge flower bud bacterium bag later to choose carrying out two sections into the fertility chamber and urge flower bud to handle; Reduce temperature to 19～22 ℃, humidity 95～99% increases illumination; The alveole edge for the treatment of the former primary surface of grifola frondosus grows linen projection gradually, and differentiates stigma one by one rapidly, and whole original hase urges flower bud to finish for two sections as coral at this moment;F) vegetative period: this stage keeps 19～21 ℃ of temperature, reduces space CO 2Concentration is below 1000ppm; Original hase is divided into fan-shaped blade gradually, and color shoals; When treating that fine aperture appears in the grifola frondosus vacuum side of blade, maturation is described, but timely collecting.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106605525A (en) * | 2016-08-10 | 2017-05-03 | 上海雪榕生物科技股份有限公司 | Control method of maitake production environment |
| CN106797805A (en) * | 2017-03-17 | 2017-06-06 | 北京市农业技术推广站 | A kind of chestnut mushroom exempts from soil fruiting cultivation technique |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101715696A (en) * | 2009-12-18 | 2010-06-02 | 上海市农业科学院 | Factory cultivation method of maitake |
| CN101731097A (en) * | 2009-12-18 | 2010-06-16 | 上海市农业科学院 | Grifola frondosus liquid strain and method for cultivating grifola frondosus by using liquid strain |
-
2010
- 2010-08-12 CN CN 201010251549 patent/CN101946631A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101715696A (en) * | 2009-12-18 | 2010-06-02 | 上海市农业科学院 | Factory cultivation method of maitake |
| CN101731097A (en) * | 2009-12-18 | 2010-06-16 | 上海市农业科学院 | Grifola frondosus liquid strain and method for cultivating grifola frondosus by using liquid strain |
Non-Patent Citations (2)
| Title |
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| 《农村实用技术》 20080430 陈亚光等 灰树花熟料袋栽技术 , 第04期 2 * |
| 《浙江食用菌》 20100630 吴银华等 玉米芯栽培灰树花试验初报 第18卷, 第3期 2 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106605525A (en) * | 2016-08-10 | 2017-05-03 | 上海雪榕生物科技股份有限公司 | Control method of maitake production environment |
| CN106797805A (en) * | 2017-03-17 | 2017-06-06 | 北京市农业技术推广站 | A kind of chestnut mushroom exempts from soil fruiting cultivation technique |
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Open date: 20110119 |