CN108040740A - A kind of Cordceps militaris potting solid Industry Cultivation integration system and application - Google Patents

A kind of Cordceps militaris potting solid Industry Cultivation integration system and application Download PDF

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Publication number
CN108040740A
CN108040740A CN201711160993.9A CN201711160993A CN108040740A CN 108040740 A CN108040740 A CN 108040740A CN 201711160993 A CN201711160993 A CN 201711160993A CN 108040740 A CN108040740 A CN 108040740A
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China
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cordceps militaris
basin
along
basin body
potting
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侯绪营
张劲松
王小印
洪春艳
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WULUMUQI RESEARCH INSTITUTE OF EDIBLE MUSHROOMS
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WULUMUQI RESEARCH INSTITUTE OF EDIBLE MUSHROOMS
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Abstract

The present invention discloses a kind of Cordceps militaris potting solid Industry Cultivation integration system and application, the cultivation of Cordceps militaris is combined with the cultivation of potting mobile three-dimensional, by using the requirement of the particular technology of Cordceps militaris itself culture medium and cultivation matrix, the potting device of offer, in applicable Cordceps militaris culture cultivation, coordinated using basin body and basin lid male and female button so that basin body and basin lid effectively prevent extraneous air to be directly entered cordyceps culturing medium environment, secondly the ventilating mechanism in basin lid advantageously ensures that the temperature of cordyceps culturing medium in basin, humidity is so as to ensureing the balance of Cordceps militaris growing environment, traditional edible mushroom domestic culture device is effectively overcome since ingress of air or solution matter easily aoxidize, change colour so as to cause edible mushroom, deformation and it is dull, the technical issues of so as to seriously affect the nutrition of Cordceps militaris and mouthfeel.It realizes small, pollution-free, the easy moisturizing of Cordceps militaris floor space, realizes Cordceps militaris from professional production to the transformation of amateur production.

Description

A kind of Cordceps militaris potting solid Industry Cultivation integration system and application
Technical field
The present invention relates to the technical field of edible fungus culturing, in particular to the technical fields of edible Cordceps militaris bowl cultivation.
Background technology
Cordceps militaris is the abbreviation of northern Chinese caterpillar Fungus, is also Cordyceps militaris or pupa grass, is the medicinal fungi that bacterium combines.It mainly gives birth to Grow the northern area in China.Cordceps militaris contains abundant protein, 18 kinds of amino acid, 17 kinds of trace elements, 12 kinds of dimension lifes Element：A、B1、B2、B6、B12, C, D, E equal size higher than mushroom class, be first-class excellent tonic product.
Domestic and international many experts are to nourishing Northern cordyceps chemical analysis, the research of pharmacology and clinical effectiveness, substantially in Magnificent cordyceps sinensis is identical, mainly has and protects lung kidney-nourishing, and hemostasis and phlegm adjusts physical function, and strengthen immunity cures mainly pulmonary tuberculosis hemoptysis, sun It withers seminal emission, soreness of waist and knee joint, beauty and skin care, psychasthenia, diabetes rise white blood cell, anti-cancer, anticancer, to Chemotherapy also very Greatly.The effect of Cordceps militaris medicinal efficacy of artificial cultivation is with wild Cordceps militaris is very nearly the same, some performances are better than wild Cordceps militaris. With social development, the reinforcement of the health care consciousness of people, the edible value and medical value that artificial cordyceps have are deep by vast The welcome of consumer will have a vast market prospect.
Cordyceps militaris cultivation method is usually used bed cultivation, outdoor plantation, the plantation of material bag disjunctor or bottle and plants at present, is planted using bed Or the cultivation of the edible mushroom of outdoor plantation belongs to open place, such planting type is not easy to be mended in the course of cultivation Water, affected by environment big, yield is unstable；Secondly it is easy to pollute, be subject to disease pest infringement, biological transformation ratio is low, sick in order to prevent Insect pest to mushroom applying pesticides, will form pollution, reduce product quality；In addition it is big to take up an area face, is unfavorable for extensive and factory Change cultivation.It can be seen that traditional Cordceps militaris is cultivated, such as bottle is planted, plastic bag cultivation be it is simple provide carrier to cultivate raw material, sell with Based on cordyceps product, marketing method is single, is unfavorable for the popularization and sale of Cordceps militaris.Therefore there is an urgent need for a kind of floor space is small, nothing Pollution, easy moisturizing, biological transformation ratio be high, can on a large scale and factory culture, and the training of the Cordceps militaris with new cultivation mode Educate technology.
The content of the invention
It is cultivated for the Cordceps militaris of objective reality in the prior art using bed cultivation, outdoor plantation, the plantation of material bag disjunctor or bottle Plant, be not easy to carry out moisturizing in the course of cultivation, affected by environment big, yield is unstable, while existing cultivation system easily pollute, Disease pest infringement is subject to, biological transformation ratio is low, in order to prevent pest and disease damage, to cordyceps sinensis applying pesticides, will form pollution, reduce production Quality；In addition it is big to take up an area face, it is impossible to realize extensive and factory culture the state of the art.The present invention intends to provide one kind Cordceps militaris potting solid Industry Cultivation integration system and application, so as to fulfill Cordceps militaris cultivate cultivation in floor space it is small, without dirt Dye, easy moisturizing, biological transformation ratio are high, can realize high yield and high quality factory culture on a large scale, obtain significant technique effect.
The present invention provides a kind of Cordceps militaris potting solid Industry Cultivation integration system, and the culture utilization potting of Cordceps militaris is moved Dynamic formula stereo cultivation is combined, and is placed in by using cordyceps culturing medium in potting stereoscopic cultivating device, is realized and is carried out in factory Scale cultivates the high-quality guarantee of Cordceps militaris high yield fruiting.The present situation of the invention for effectively overcoming the difficult culture of Cordceps militaris, using Cordceps militaris certainly The requirement of the particular technology of body culture medium and cultivation matrix, a kind of creative basin for being suitable for Cordceps militaris solid Industry Cultivation of offer Device is planted, in applicable Cordceps militaris culture cultivation, is coordinated using basin body and basin lid male and female button so that basin body and basin lid have Effect prevents extraneous air to be directly entered cordyceps culturing medium environment, and secondly the ventilating mechanism in basin lid advantageously ensures that northern worm in basin The epidemic disaster of careless culture medium is so as to ensure the balance of Cordceps militaris growing environment, and in addition ventilating mechanism top is higher by basin lid surface and sets It puts, the equal flush in ventilating mechanism top is set, and does not influence basin body ventilation also so that the solid heap of the present apparatus stacks to put, and is protected Card cultivates the quality of Cordceps militaris, is set on basin body and increases layer, facilitated the cultivation of different height Cordceps militaris, and grown according to Cordceps militaris Cultivating techniques need, and design had both met laboratory Cordceps militaris and cultivated, while effectively overcome traditional edible mushroom domestic culture dress Put due to ingress of air or solution matter easily aoxidize, so as to cause edible mushroom discoloration, deformation and it is dull, so as to seriously affect north The technical issues of nutrition of cordyceps sinensis and mouthfeel.
Specifically, the present invention provides a kind of Cordceps militaris potting solid Industry Cultivation integration system, specific integration system includes Below：
(1) culture medium is prepared：Culture medium contains dry without the wheat, clear water and the nutrient solution that go mouldy；The weight of wheat and water Than for 1:(1.3-2.0)；Nutrient solution contains glucose, protein adhesive, carbonic acid potassium dihydrogen, magnesium sulfate, vitamin B2, vitamin B12。
(2) feed：Nutrient solution is added in after wheat is proportionally mixed with clear water, loading is stirred evenly and connects Cordceps militaris strain Basin body device in, and on the basin body device for connect Cordceps militaris strain cover a polybag.
(3) high pressure sterilization：High pressure sterilization is carried out, is placed on after being cooled down in desinfection chamber and prepares inoculation.
(4) transfer room sterilizes：The disinfection of indoor vacant room and disinfection in kind, are inoculated with after disinfection, bacterium solution need to use sterile water into The weight ratio of row dilution, bacterium solution and sterile water is 2:5.
(5) culturing room sterilizes：Culturing room's carrying out disinfection for 3-4 days before culture medium is moved into.
(6) cultural hypha：Culture medium is moved into the culturing room of thorough disinfection, the strain being inoculated with is emitted on shelf On, in the bacterium germination stage into trip temperature and the adjusting of illumination.
(7) management of flower bud and fructification：Flower bud goes out during stroma, control scattering illumination and night light application time, room Interior epidemic disaster and the ventilation frequency.
Further, the present invention provides full and accurate Cordceps militaris potting solid Industry Cultivation integrated approach system, specific conglomerate System includes following：
(1) culture medium is prepared：Culture medium contains dry without the wheat, clear water and the nutrient solution that go mouldy；Nutrient solution contains following Raw materials by weight meter is prepared, glucose 66.18%-79.1%, protein adhesive 8.72%-13.08%, carbon Acid dihydride potassium 8.72%-13.08%, magnesium sulfate 3.42%-7.62%, vitamin B20.01%-0.02%, vitamin B120.01%-0.02%.
(2) feed：By wheat and clear water according to weight ratio 1:Nutrient solution is added in after (1.3-2.0) mixing, stirs evenly dress In the basin body device for entering to connect Cordceps militaris strain, and a polybag is covered on the basin body device for connect Cordceps militaris strain.
(3) high pressure sterilization：Cold air is emptied, pressure starts to calculate sterilization time, thermometer 120- when being raised to 1.5 kilograms Stove is removed after being kept for 30-40 minutes at 125 degree, starts to cool down, naturally rings to slowly open air bleeding valve during 1 kilogram of pressure, is arranged It boils after to pressure back to zero, to be opened slowly when boiling at once, at least cooling down 20 minutes takes the dish out of the pot, and is placed on after being cooled down in desinfection chamber and prepares to connect Kind.
(4) transfer room sterilizes：Indoor vacant room disinfection and disinfection in kind；3*5 square metres of the careat of transfer room, four is careful Envelope, the door of transfer room must have surge chamber；Not into progress Interior Space room disinfection before raw material, when using spray lysol 2 small first, lysol is every Cubic meter 15ml is watered 500ml, and the culture medium to have sterilized is moved into after then being sterilized 30-60 minutes with 10A ozone machines；Material object disappears Poison into before inoculation, replaces work clothes in surge chamber, cap, mask, and shoe cover will be connect in the basin body device of Cordceps militaris strain Bacterium basin body is taken again with disinfectant wash clean into transfer room, into transfer room after electronic inoculator is started, alcohol is allowed to spray 2-3 point Clock pours out residual alcohol, then multiple with sterile water wash pipeline, and strain is added in after clean；Northern worm is moved into after finished in next step Careless culturing room is cultivated, and must keep completely black light culture within first 7 days；Be inoculated with after disinfection, bacterium solution need to use sterile water into The weight ratio of row dilution, bacterium solution and sterile water is 2:5.
(5) culturing room sterilizes：Culturing room carries out thorough disinfection in 3-4 days before culture medium is moved into ozone machine.
(6) cultural hypha：Culture medium is moved into the culturing room of thorough disinfection, the basin for the Cordceps militaris strain being inoculated with In body device bacterium basin discharge on the top of the shelf, the bacterium germination stage preceding 4-5 days indoor temperatures control between 15-18 DEG C, daily into Row ventilation by forced draft 30 minutes；It can be appreciated that strain is sprouted after 2 days, cover within 5-7 days bacterium, improve temperature and be adjusted between 18-22 DEG C, Humid control carries out ventilation by forced draft 2 times 30 minutes daily between 65-70%, is adjusted according to indoor actual conditions are cultivated；Through The culture of 10-15 days is crossed, Chinese caterpillar fungus hypha covers with full basin；Give daily 20-24 it is small when illumination, by the scattering illumination of 4-5 days, When surface switchs to reddish yellow, mycelia nutrient growth is ripe.
(7) management of flower bud and stroma：Flower bud goes out during stroma, daily scatter illumination be no less than 8 it is small when, at night will also With solar radiation 16 it is small when；20-22 DEG C of indoor temperature reduces 5-8 DEG C at night；Indoor air humidity is controlled 70%；It is logical daily Gas 2-3 times, ventilation 20-40 minutes, twist together into after mycelia switchs to crocus by white and successively cima shape protuberance former base every time, When secreting yellow droplet, the preparation of long stroma of Cordyceps militaris is just carried out；Go out the humidity of stroma management, cordyceps sinensis go out before 6-7 days, Room temperature is controlled at 18-20 DEG C, and later room temperature improves 2 DEG C, but no more than 25 DEG C；Sufficient scattering light, but it is unable to direct light According to；Humidity is increased to 85%, when Cordceps militaris planar projection forms small rice grain former base, ventilation supplement fresh air；Cordyceps sinensis grows 2 After centimetre is long, indoor humidity is increased to 90%, wall water spray in space strengthens ventilating management to improve humidity；The long 3 centimeters of height of grass Afterwards, illumination be reduced to daily 10-12 it is small when, when 6-4 is small, temperature difference 3-5 degree, between humidity 85%-90%；It gives within 3-5 days before harvesting Give 20 it is small when illumination Cordceps militaris color and luster it is good, ventilating management can harvest after 50-60 days.
In the present invention, the potting device of the basin body device for connecing Cordceps militaris strain, i.e. Cordceps militaris solid Industry Cultivation of use Including basin body and basin lid, basin body top set it is horizontal along one, it is horizontal set along one it is vertical along one, it is vertical orthogonal to be set along one with horizontal stroke along one Put, basin lid bottom set it is horizontal along two, it is horizontal set along two it is vertical along two, indulge along two with horizontal along two orthogonal settings, it is horizontal along one with Horizontal stroke is adapted along two and sets, and indulges and is set along one and vertical be adapted along two, indulges along two upper ends and set top layer, groove is set on top layer, Mounting ring is set in groove, and mounting ring one end is arranged on outside basin lid, and the mounting ring other end is arranged in basin lid, and mounting ring is equipped with Ventilating mechanism, in ventilating mechanism, mounting ring lower end is equipped with air hole bottom cover, is equipped between air hole bottom cover and mounting ring ventilative Film, mounting ring upper end are equipped with air hole upper cover, and air hole upper cover side sets the bottom of upper air hole, ventilating mechanism top and basin body Portion's flush is set.
In the present invention, support ring preferentially is set along base using in air hole bottom cover, support ring is adapted with mounting ring and sets It puts, ventilated membrane is arranged between support ring and mounting ring, and support column, the height and support ring of support column are set in air hole bottom cover Height it is identical.
It is preferential that setting between basin body and basin lid is used to increase layer in the present invention, increase layer upper end and protrusion one, protrusion one are set It is set with vertical be adapted along two, increases layer lower end and groove one is set, groove one and vertical be adapted along one are set, groove one and protrusion One is adapted setting.
In the present invention, preferential use increases the height of layer as 3cm-10cm.
In the present invention, the preferential lower air hole using on air hole bottom cover is along air hole bottom cover geometric center circumferentially array It sets, along air hole upper cover axis, circumferentially array is set the upper air hole on air hole on lid.
In the present invention, preferentially set using the base of upper air hole higher than the upper surface of basin lid.
It it is preferentially 12 using the quantity of upper air hole and lower air hole in the present invention.
It is preferential that groove is respectively provided with using the surrounding of top layer in the present invention.
It is preferential that through hole is set along one using horizontal stroke in the present invention, it is horizontal that the protrusion being adapted with through hole, through hole are set along two Quantity to be more than two, through hole is arranged on the both sides of basin body.
In the present invention, transparent material is preferentially used using arc surface, basin body and basin lid using base in basin body, basin body uses Cube, cuboid, cylinder, Elliptic Cylinder shape.
The present invention preferentially selects fresh nothing to go mouldy, free from insect pests, the wheat without pesticide and other chemicals is as raw material.
The preferential dispensing for selecting nutrient solution of the invention, each material component matches by weight percentage is：Glucose 72.64%th, protein adhesive 10.90%, carbonic acid potassium dihydrogen 10.90%, magnesium sulfate 5.52%, vitamin B20.02%th, vitamin B120.02%.
Cordceps militaris of the present invention is the technical foundation of aerobic fungi, using the basin of the Cordceps militaris solid Industry Cultivation of above-mentioned offer Plant device, it can be ensured that grown in scarlet caterpiller fungus mycelium and go out in stroma growth course to be required to fully ensure that the ring of ventilation Border is allowed to keep good growth conditions.Scarlet caterpiller fungus mycelium growth phase respiratory capacity is less, and room ventilation number can suitably subtract It is few, but to ensure to cultivate room air freshness, CO2Concentration maintains 0.05%~0.03%；Go out during stroma to need more The frequency is largely aerated, to exclude CO in bacterium basin2Concentration ensures the normal development of stroma.If cultivate chamber ventilated not It is good, when gas concentration lwevel is excessive, easily lead to the death of prematurity stroma.This is to have no to be suitble to north in currently available technology field Cordyceps sinensis realizes the basin body of potting or is suitable for realizing the device of potting cultivating system.
The present invention takes into full account that Cordceps militaris belongs to middle cryogenic thermostat type mushroom, three-dimensional according to the Cordceps militaris potting of above-mentioned offer Industry Cultivation integrated approach system is applicable in the potting device provided, and scarlet caterpiller fungus mycelium growth phase temperature range is 15-28 DEG C, optimum temperature is 15-20 DEG C；Stroma growth phase temperature range is 8-25 DEG C, and optimum temperature is 18-22 DEG C.
In the present invention, Cordceps militaris scattered daily during flower bud goes out stroma illumination be no less than 8 it is small when, still need at night With solar radiation 16 it is small when, 20-22 DEG C of indoor temperature, at night reduce 5-8 DEG C；Indoor air humidity is controlled 70%；It is logical daily Gas 2-3 times, ventilation 20-40 minutes, it is former to twist together into after mycelia switchs to crocus by white and successively cima shape protuberance every time Base when secreting yellow droplet, just carries out the preparation of long stroma of Cordyceps militaris.
In the present invention, Cordceps militaris is cultivated 50-60 days or so, during the long 6-10cm high of grass, sees that careless discoloration is shallow, culture medium dehydration receipts Contracting, with hand getting when, are easy to wheat separation, mean that Cordceps militaris to the maturity period, can be picked.
By implementing technical scheme, following advantageous effect can be reached：
(1) cultivation of Cordceps militaris is combined to form Industry Cultivation integration system by the present invention with three-dimensional potting, Cordceps militaris training In requirement technical characteristic with of the present invention bacterium basin of the management phase to humiture and gas of cultural hypha, flower bud and stroma in educating It is adapted, can ensure that the three-dimensional interior environment stacked in cultivating process in basin carries out good cycling with culture room environmental, make basin Interior interior environment reaches unanimity with culture room environmental, ensures the demand of Cordceps militaris epidemic disaster and aerobic conditions, while can be effective It prevents the intrusion of novel bacteria and dust, reduces cultivation pollution rate, while ensureing product quality, solid cultivation efficiently solves Occupation of land problem is conducive to the production of extensive and factory culturing.
(2) present invention to the Cordceps militaris cultivated under potting solid Industry Cultivation integration system by carrying out sensory evaluation and sound The technical controlling means for answering face method and function test etc. comprehensive and verification, obtain optimal parameter in Cordceps militaris cultivating process：Bacterium germination Phase temperature is 15-18 DEG C, strain sprouting phase temperature is 18-22 DEG C, flower bud goes out stroma phase temperature and is 20-22 DEG C, goes out son Seat phase temperature is 18-20 DEG C；It is obtained by further preferred verification experimental verification, the bacterium germination phase temperature of Cordceps militaris is 16.5 DEG C, Strain sprouts that phase temperature is 20 DEG C, flower bud goes out that stroma phase temperature is 21 DEG C, to go out stroma phase temperature be 19 DEG C.
(3) Cordceps militaris potting solid Industry Cultivation of the invention integrates cultivation system and meets the ecological, environmental protective that country advocates, The production of bacterium basin will not can be caused environment pollution as plastic bag cultivation.It is the polypropylene material for meeting medical standard that the present invention, which selects, Material, and it is reusable, it will not cause environment pollution.
(4) Cordceps militaris potting solid Industry Cultivation of the invention, which integrates cultivation system, only needs operating personnel to be put into basin body Culture medium of Cordyceps militaris cultivates cordyceps sinensis in factory after inoculation and grows to a certain size, to peasant or can dissipate under suitable culture environment Yang Hu families are sold, it is not necessary to be sold again when cordyceps sinensis maturation, this opens new sale mould for the popularization and application of Cordceps militaris Formula.
(5) Cordceps militaris potting solid Industry Cultivation of the invention, which integrates cultivation system, realizes Cordceps militaris professional production to non- The leap of professional production can allow and the interested citizen of Cordceps militaris can voluntarily be cultivated, and the full mistake of the growth for experiencing Cordceps militaris Journey enjoys green, safe, delicious good product.
Description of the drawings
Fig. 1 is shown as the structure diagram of the present invention.
Fig. 2 is shown as basin body structure schematic diagram provided by the invention.
Fig. 3 is shown as basin lid structure diagram provided by the invention.
Fig. 4 is shown as breather hole cover structure diagram provided by the invention.
Fig. 5 is shown as provided by the invention and increases layer structure schematic diagram.
Fig. 6 is shown as use state diagram provided by the invention.
Fig. 7 is shown as bacterium germination phase temperature and strain sprouts phase temperature to being trained under potting solid Industry Cultivation integration system The Cordceps militaris sensory evaluation response surface graph educated.
Fig. 8 is shown as bacterium germination phase temperature and flower bud goes out stroma phase temperature under potting solid Industry Cultivation integration system The Cordceps militaris sensory evaluation response surface graph of cultivation.
Fig. 9 is shown as bacterium germination phase temperature and goes out stroma phase temperature to being cultivated under potting solid Industry Cultivation integration system Cordceps militaris sensory evaluation response surface graph.
Figure 10 is shown as strain sprouting phase temperature and flower bud goes out stroma phase temperature and potting solid Industry Cultivation is integrated The Cordceps militaris sensory evaluation response surface graph cultivated under system.
Figure 11 is shown as strain and sprouts phase temperature and go out stroma phase temperature to potting solid Industry Cultivation integration system The Cordceps militaris sensory evaluation response surface graph of lower cultivation.
Figure 12 is shown as flower bud and goes out stroma phase temperature and go out stroma phase temperature to potting solid Industry Cultivation conglomerate The lower Cordceps militaris sensory evaluation response surface graph cultivated of system.
In figs. 1-6：1-basin body, 11-horizontal along one, 111-through hole, 12-it is vertical along one, 13-interior base, 2-basin Lid, 21-horizontal along two, 211-protrusion, 22-vertical along two, 23-top layer, 24-groove, 241-mounting ring, 3-ventilating machine Structure, 31-air hole bottom cover, 311-lower air hole, 312-support column, 313-support ring, 32-ventilated membrane, 33-ventilative Hole upper cover, 331-upper air hole, 4-increase layer, 41-groove one, 42-protrusion one.
Specific embodiment
In the present invention, for ease of description, in the potting device of Cordceps militaris solid Industry Cultivation, each component it is opposite The description of position relationship is with reference to the accompanying drawings 1 Butut mode to be described, such as：The position relationships such as upper and lower, left and right be according to Come according to the Butut direction of attached drawing 1 definite, the basin body device of Cordceps militaris strain is connect in the present invention also referred to as " bacterium basin ".
The ventilated membrane (32) that the present invention uses can be bought or be customized by public channel.
Wheat that the present invention uses, glucose, protein adhesive, carbonic acid potassium dihydrogen, magnesium sulfate, vitamin B2, vitamin B12Deng Can be bought by public channel, the Cordceps militaris strain employed in technique can by public's channel buy obtain production bacterium or from Row cultivates screening and obtains production strain.
What all material, reagent and the instrument selected in the present invention were all well known in the art, but the reality of the present invention is not limited It applies, other some reagents well known in the art and equipment are applied both to the implementation of implementation below of the present invention.
Embodiment one：
A kind of Cordceps militaris potting solid Industry Cultivation integration system, the technical solution of specific integration system is including following：
(1) culture medium is prepared：Culture medium contains dry without the wheat, clear water and the nutrient solution that go mouldy；Nutrient solution contains following Raw materials by weight meter is prepared：Glucose 66.18%-79.1%, protein adhesive 8.72%-13.08%, carbon Acid dihydride potassium 8.72%-13.08%, magnesium sulfate 3.42%-7.62%, vitamin B20.01%-0.02%, vitamin B120.01%-0.02%.
(2) feed：By wheat and clear water according to weight ratio 1:Nutrient solution is added in after (1.3-2.0) mixing, stirs evenly dress In the basin body device for entering to connect Cordceps militaris strain, and a polybag is covered on the basin body device for connect Cordceps militaris strain.
(3) high pressure sterilization：Cold air is emptied, pressure starts to calculate sterilization time, thermometer 120- when being raised to 1.5 kilograms Stove is removed after being kept for 30-40 minutes at 125 degree, starts to cool down, naturally rings to slowly open air bleeding valve during 1 kilogram of pressure, is arranged It boils after to pressure back to zero, to be opened slowly when boiling at once, at least cooling down 20 minutes takes the dish out of the pot, and is placed on after being cooled down in desinfection chamber and prepares to connect Kind.
(4) transfer room sterilizes：Indoor vacant room disinfection and disinfection in kind；3*5 square metres of the careat of transfer room, four is careful Envelope, the door of transfer room must have surge chamber；Not into progress Interior Space room disinfection before raw material, when using spray lysol 2 small first, lysol is every Cubic meter 15ml is watered 500ml, and the culture medium to have sterilized is moved into after then being sterilized 30-60 minutes with 10A ozone machines；Material object disappears Poison into before inoculation, replaces work clothes in surge chamber, cap, mask, and shoe cover takes bacterium basin body with disinfectant wash clean again Enter transfer room, into transfer room after electronic inoculator is started, alcohol is allowed to spray 2-3 minutes, pours out residual alcohol, then with sterile Water detergent line is multiple, and strain is added in after clean；Cordceps militaris culturing room is moved into after finished in next step to be cultivated, preceding 7 It must keep completely black light culture；It is inoculated with after disinfection, bacterium solution need to be diluted with sterile water, the weight of bacterium solution and sterile water Than for 2:5.
(5) culturing room sterilizes：Culturing room carries out thorough disinfection in 3-4 days before culture medium is moved into ozone machine.
(6) cultural hypha：Culture medium is moved into the culturing room of thorough disinfection, the bacterium basin being inoculated with is emitted on shelf On, it is controlled in the preceding 4-5 days indoor temperatures in bacterium germination stage between 15-18 DEG C, carries out ventilation by forced draft 30 minutes daily；Normal 2 It can be appreciated that strain is sprouted after it, cover within general 5-7 days, improve temperature and be adjusted between 18-22 DEG C, humid control is in 65-70% Between, ventilation by forced draft 2 times 30 minutes is carried out daily, is adjusted according to indoor actual conditions are cultivated；By the culture of 10-15 days, Chinese caterpillar fungus hypha covers with full basin；Give daily 20-24 it is small when illumination, by the scattering illumination of 4-5 days, surface switched to reddish yellow When, mycelia nutrient growth is ripe.
(7) management of flower bud and stroma：Flower bud goes out during stroma, daily scatter illumination be no less than 8 it is small when, at night will also With solar radiation 16 it is small when；20-22 DEG C of indoor temperature reduces 5-8 DEG C at night；Indoor air humidity is controlled 70%；It is logical daily Gas 2-3 times, ventilation 20-40 minutes, twist together into after mycelia switchs to crocus by white and successively cima shape protuberance former base every time, When secreting yellow droplet, the preparation of long stroma of Cordyceps militaris is just carried out；Go out the humidity of stroma management, cordyceps sinensis go out before 6-7 days, Room temperature is controlled at 18-20 DEG C, and later room temperature improves 2 DEG C, but no more than 25 DEG C；Sufficient scattering light, but it is unable to direct light According to；Humidity is increased to 85%, when Cordceps militaris planar projection forms small rice grain former base, ventilation supplement fresh air；Cordyceps sinensis grows 2 After centimetre is long, indoor humidity is increased to 90%, wall water spray in space strengthens ventilating management to improve humidity；The long 3 centimeters of height of grass Afterwards, illumination be reduced to daily 10-12 it is small when, when 6-4 is small, temperature difference 3-5 degree, between humidity 85%-90%；It gives within 3-5 days before harvesting Give 20 it is small when illumination Cordceps militaris color and luster it is good, ventilating management can harvest after 50-60 days.
Embodiment two：
The culture medium of Cordceps militaris contains wheat, clear water and nutrient solution；Nutrient solution contains glucose 4.63kg, protein adhesive 0.61kg, carbonic acid potassium dihydrogen 0.61kg, magnesium sulfate 0.24kg, vitamin B20.4g, vitamin B120.7g；Wheat and clear water are pressed According to weight ratio 1:Nutrient solution is added in after 1.3 mixing, stirs evenly in the basin body device for being fitted into and connecing Cordceps militaris strain, and is connecing northern worm A polybag is covered on the basin body device of careless strain.
Pressure is raised to 1.5 kilograms when carrying out high pressure sterilization, and temperature is kept for 30 minutes at 120 DEG C, then removes stove, starts Cooling, naturally rings to slowly open air bleeding valve during 1 kilogram of pressure, drains into after pressure back to zero the 20 minutes horse backs that cool down and boils, is placed on Prepare inoculation after being cooled down in desinfection chamber；Transfer room disinfection is carried out, not into progress Interior Space room disinfection before raw material, first with spray lysol 2 it is small when, every cubic metre of 15ml of lysol is watered 500ml, and the culture that has sterilized is moved into after then being sterilized 30 minutes with 10A ozone machines Base；Material object disinfection, into before inoculation, replaces work clothes in surge chamber, cap, mask, and shoe cover washes bacterium basin body with disinfectant Totally taken again into transfer room, into transfer room after electronic inoculator is started, allow alcohol spray 2-3 minutes, pour out residual alcohol, It is multiple with sterile water wash pipeline again, add in strain after clean；The culture of Cordceps militaris culturing room is moved into after finished in next step to carry out Culture, must keep completely black light culture in first 7 days；Be inoculated with after disinfection, bacterium solution need to be diluted with sterile water, bacterium solution with it is sterile The weight ratio of water is 2:5；Culturing room sterilizes, culturing room thoroughly being disappeared with ozone machine for 3-4 days before culture medium is moved into Poison；Culture medium is moved into the culturing room of thorough disinfection by cultural hypha, the bacterium basin discharge being inoculated on the top of the shelf, is being sent out The preceding 4-5 days indoor temperatures in bacterium stage are controlled between 15 DEG C, carry out ventilation by forced draft 30 minutes daily；It can be appreciated that after normal 2 days Strain is sprouted, and is covered within general 5-7 days, is improved temperature and is adjusted between 18 DEG C, humid control carries out convection current daily between 65% Ventilation 2 times 30 minutes is adjusted according to indoor actual conditions are cultivated；By the culture of 10-15 days, Chinese caterpillar fungus hypha covered with full basin； Give daily 20-24 it is small when illumination, by the scattering illumination of 4-5 days, when surface switchs to reddish yellow, mycelia nutrient growth into It is ripe；The management of flower bud and stroma, flower bud go out during stroma, daily scatter illumination be no less than 8 it is small when, at night also use day illumination Penetrate 16 it is small when；20 DEG C of indoor temperature reduces by 5 DEG C at night；Indoor air humidity is controlled 70%；Ventilation 2-3 times daily, it is logical every time Gas 20-40 minutes twists together into after mycelia switchs to crocus by white and successively cima shape protuberance former base, secretes yellow droplet When, carry out out stroma management；Go out the humidity of stroma management, cordyceps sinensis go out before 6-7 days, room temperature is controlled at 18 DEG C, and later room temperature carries It is 2 DEG C high, but no more than 25 DEG C；Sufficient scattering light, but it is unable to direct light photograph；Humidity is increased to 85%, treats Cordceps militaris plane When protrusion forms small rice grain former base, ventilation supplement fresh air；Cordyceps sinensis grow it is 2 centimeters long after, indoor humidity is increased to 90%, wall water spray in space strengthens ventilating management to improve humidity；After the long 3 centimeters of height of grass, it is small that illumination is reduced to daily 10-12 When, when 6-4 is small, temperature difference 3-5 degree, between humidity 85%；Give within 3-5 days before harvesting 20 it is small when illumination Cordceps militaris color and luster it is good, lead to Air hose can harvest after managing 50-60 days.
Embodiment three：
The culture medium of Cordceps militaris contains wheat, clear water and nutrient solution；Nutrient solution contains glucose 5.53kg, protein adhesive 0.92kg, carbonic acid potassium dihydrogen 0.92kg, magnesium sulfate 0.53kg, vitamin B21.4g, vitamin B121.4g；Wheat and clear water are pressed According to weight ratio 1:Nutrient solution is added in after 2.0 mixing, stirs evenly in the basin body device for being fitted into and connecing Cordceps militaris strain, and is connecing northern worm A polybag is covered on the basin body device of careless strain；When carrying out high pressure sterilization, pressure is raised to 1.5 kilograms, and temperature is in 125 DEG C of guarantors It holds 35 minutes, then removes stove, start to cool down, naturally ring to slowly open air bleeding valve during 1 kilogram of pressure, drain into pressure back to zero The 20 minutes horse backs that cool down afterwards boil, and are placed on after being cooled down in desinfection chamber and prepare inoculation；Transfer room disinfection is carried out, not into carrying out before raw material Indoor vacant room disinfection, when using spray lysol 2 small first, every cubic metre of 15ml of lysol is watered 500ml, is then sterilized with 10A ozone machines The culture medium to have sterilized is moved into after 30-60 minutes；Material object disinfection, into before inoculation, replaces work clothes in surge chamber, cap, Mask, shoe cover take bacterium basin body again into transfer room with disinfectant wash clean, into transfer room after electronic inoculator is started, allow Alcohol sprays 2-3 minutes, pours out residual alcohol, then multiple with sterile water wash pipeline, and strain is added in after clean；Under after finished One step is moved into Cordceps militaris culturing room and is cultivated, and must keep completely black light culture within first 7 days；It is inoculated with after disinfection, bacterium solution It need to be diluted with sterile water, the weight ratio of bacterium solution and sterile water is 2:5；Culturing room sterilizes, and culturing room moves into it in culture medium Preceding 3-4 days carry out thorough disinfection with ozone machine；Culture medium is moved into the culturing room of thorough disinfection by cultural hypha, having connect The bacterium basin discharge planted on the top of the shelf, controls between 18 DEG C in the preceding 4-5 days indoor temperatures in bacterium germination stage, carries out convection current daily Ventilation 30 minutes；It can be appreciated that strain is sprouted after normal 2 days, cover within general 5-7 days, improve temperature and be adjusted between 22 DEG C, humidity Control carries out ventilation by forced draft 2 times 30 minutes daily between 70%, is adjusted according to indoor actual conditions are cultivated；By 10-15 It culture, Chinese caterpillar fungus hypha cover with full basin；Give daily 20-24 it is small when illumination, by the scattering illumination of 4-5 days, surface turned For reddish yellow when, mycelia nutrient growth is ripe；The management of flower bud and stroma, flower bud go out during stroma, scatter illumination not daily When small less than 8, when also using solar radiation 16 small at night；22 DEG C of indoor temperature is appropriate at night to reduce by 8 DEG C；Indoor air humidity Control is 70%；Ventilation 2-3 times daily, ventilation 20-40 minutes, twist together after mycelia switchs to crocus by white and successively every time Former base is swelled into cima shape, when secreting yellow droplet, carries out out stroma management；Go out the humidity of stroma management, cordyceps sinensis go out before 6-7 My god, room temperature is controlled at 20 DEG C, and later room temperature improves 2 DEG C, but no more than 25 DEG C；Sufficient scattering light, but it is unable to direct light photograph； Humidity is increased to 85%, when Cordceps militaris planar projection forms small rice grain former base, ventilation supplement fresh air；It is public that cordyceps sinensis grows 2 After point long, indoor humidity is increased to 90%, wall water spray in space strengthens ventilating management to improve humidity；The long 3 centimeters of height of grass Afterwards, illumination be reduced to daily 10-12 it is small when, when 6-4 is small, temperature difference 3-5 degree, between humidity 90%；Give 20 within 3-5 days before harvesting The Cordceps militaris color and luster of hour illumination is good, and ventilating management can harvest after 50-60 days.
Example IV：
The culture medium of Cordceps militaris contains wheat, clear water and nutrient solution；Nutrient solution contains glucose 5.08kg, protein adhesive 0.77kg, carbonic acid potassium dihydrogen 0.77kg, magnesium sulfate 0.39kg, vitamin B21.05g, vitamin B121.05g；By wheat and clear water According to weight ratio 1:Nutrient solution is added in after 1.5 mixing, stirs evenly in the basin body device for being fitted into and connecing Cordceps militaris strain, and is connecing north A polybag is covered on the basin body device of cordyceps species；When carrying out high pressure sterilization, pressure is raised to 1.5 kilograms, and temperature is at 123 DEG C It is kept for 40 minutes, then removes stove, start to cool down, naturally ring to slowly open air bleeding valve during 1 kilogram of pressure, drained into pressure and return The 20 minutes horse backs that cool down after zero boil, and are placed on after being cooled down in desinfection chamber and prepare inoculation；Transfer room disinfection is carried out, is not advanced into raw material The vacant room disinfection of row interior, when using spray lysol 2 small first, every cubic metre of 15ml of lysol is watered 500ml, is then disappeared with 10A ozone machines The culture medium to have sterilized is moved into after 30-60 minutes malicious；Material object disinfection, into before inoculation, replaces work clothes, cap in surge chamber Son, mask, shoe cover take bacterium basin body again into transfer room with disinfectant wash clean, into transfer room after electronic inoculator is started, Alcohol is allowed to spray 2-3 minutes, pours out residual alcohol, then it is multiple with sterile water wash pipeline, add in strain after clean；After finished It moves into Cordceps militaris culturing room in next step to be cultivated, completely black light culture must be kept within first 7 days；It is inoculated with after disinfection, bacterium Liquid need to be diluted with sterile water, and the weight ratio of bacterium solution and sterile water is 2:5；Culturing room sterilizes, and culturing room moves into culture medium 3-4 days before carry out thorough disinfection with ozone machine；Culture medium is moved into the culturing room of thorough disinfection, by cultural hypha The bacterium basin discharge being inoculated on the top of the shelf, the bacterium germination stage the control of preceding 4-5 days indoor temperatures between 17 DEG C, carry out daily pair Circulation wind 30 minutes；It can be appreciated that strain is sprouted after normal 2 days, cover within general 5-7 days, improve temperature and be adjusted between 20 DEG C, it is wet Degree control carries out ventilation by forced draft 2 times 30 minutes daily between 68%, is adjusted according to indoor actual conditions are cultivated；By 10- The culture of 15 days, Chinese caterpillar fungus hypha cover with full basin；Give daily 20-24 it is small when illumination, by the scattering illumination of 4-5 days, surface When switching to reddish yellow, mycelia nutrient growth is ripe；The management of flower bud and stroma, flower bud go out during stroma, scatter illumination daily No less than 8 it is small when, at night also with solar radiation 16 it is small when；21 DEG C of indoor temperature is appropriate at night to reduce by 6 DEG C；Interior Space air humidity Degree control is 70%；Ventilation 2-3 times daily, ventilation 20-40 minutes, are turned round after mycelia switchs to crocus by white and successively every time Form cima shape protuberance former base, when secreting yellow droplet, carry out out stroma management；Go out the humidity of stroma management, before cordyceps sinensis goes out 6-7 days, at 19 DEG C, later room temperature properly increased 2 DEG C for room temperature control, but no more than 25 DEG C；Sufficient scattering light, but cannot be straight Penetrate illumination；Humidity is increased to 85%, when Cordceps militaris planar projection forms small rice grain former base, ventilation supplement fresh air；Cordyceps sinensis Grow it is 2 centimeters long after, indoor humidity is increased to 90%, wall water spray in space strengthens ventilating management to improve humidity；Grass long 3 After centimetre is high, illumination be reduced to daily 10-12 it is small when, when 6-4 is small, temperature difference 3-5 degree, between humidity 89%；3-5 days before harvesting Give 20 it is small when illumination Cordceps militaris color and luster it is good, ventilating management can harvest after 50-60 days.
Embodiment five：
The culture medium of Cordceps militaris contains wheat, clear water and nutrient solution；Nutrient solution contain glucose 500g, protein adhesive 75g, Carbonic acid potassium dihydrogen 75g, magnesium sulfate 38g, vitamin B20.12g, vitamin B120.12g；By wheat and clear water according to weight ratio 1: Nutrient solution is added in after 1.9 mixing, is stirred evenly in the basin body device for being fitted into and connecing Cordceps militaris strain, and in the basin for connecing Cordceps militaris strain A polybag is covered on body device；When carrying out high pressure sterilization, pressure is raised to 1.5 kilograms, and temperature keeps 31 minutes at 121 DEG C, so After remove stove, start to cool down, naturally ring to slowly open air bleeding valve during 1 kilogram of pressure, drain into after pressure back to zero and cool down 20 points Clock boils at once, is placed on after being cooled down in desinfection chamber and prepares inoculation；Transfer room disinfection is carried out, is not disappeared into progress Interior Space room before raw material Poison, when using spray lysol 2 small first, every cubic metre of 15ml of lysol is watered 500ml, after then being sterilized 30-60 minutes with 10A ozone machines Move into the culture medium to have sterilized；Material object disinfection into before inoculation, replaces work clothes, cap, mask, shoe cover will in surge chamber Bacterium basin body is taken again with disinfectant wash clean into transfer room, into transfer room after electronic inoculator is started, alcohol is allowed to spray 2-3 point Clock pours out residual alcohol, then multiple with sterile water wash pipeline, and strain is added in after clean；Northern worm is moved into after finished in next step Careless culturing room is cultivated, and must keep completely black light culture within first 7 days；Be inoculated with after disinfection, bacterium solution need to use sterile water into The weight ratio of row dilution, bacterium solution and sterile water is 2:5；Culturing room sterilizes, and culturing room 3-4 days before culture medium is moved into is with smelly Oxygen machine carries out thorough disinfection；Culture medium is moved into the culturing room of thorough disinfection by cultural hypha, and the bacterium basin being inoculated with is discharged On the top of the shelf, the preceding 4-5 days indoor temperatures in the bacterium germination stage are controlled between 18 DEG C, carry out ventilation by forced draft 30 minutes daily.Just Can be appreciated that after normal 2 days strain sprout, cover within general 5-7 days, raising temperature be adjusted between 22 DEG C, humid control 69% it Between, ventilation by forced draft 2 times 30 minutes is carried out daily, is adjusted according to indoor actual conditions are cultivated；By the culture of 10-15 days, worm Careless mycelia covers with full basin.Give daily 20-24 it is small when illumination, by the scattering illumination of 4-5 days, when surface switchs to reddish yellow, Mycelia nutrient growth is ripe；The management of flower bud and stroma, flower bud go out during stroma, daily scatter illumination be no less than 8 it is small when, evening On also with solar radiation 16 it is small when；21.5 DEG C of indoor temperature reduces by 7 DEG C at night；Indoor air humidity is controlled 70%；Daily Ventilation 2-3 times, ventilation 20-40 minutes, it is former to twist together into after mycelia switchs to crocus by white and successively cima shape protuberance every time Base when secreting yellow droplet, carries out out stroma management；Go out the humidity of stroma management, cordyceps sinensis go out before 6-7 days, room temperature control exists 20 DEG C, later room temperature properly increases 2 DEG C, but no more than 25 DEG C；Sufficient scattering light, but it is unable to direct light photograph；Humidity improves To 85%, when Cordceps militaris planar projection forms small rice grain former base, ventilation supplement fresh air；Cordyceps sinensis grow it is 2 centimeters long after, will Indoor humidity is increased to 90%, and wall water spray in space strengthens ventilating management to improve humidity；After the long 3 centimeters of height of grass, illumination is reduced To daily 10-12 it is small when, when 6-4 is small, temperature difference 3-5 degree, between humidity 90%；Give within 3-5 days before harvesting 20 it is small when illumination north Cordyceps sinensis color and luster is good, and ventilating management can harvest after 50-60 days.
Embodiment six：
The culture medium of Cordceps militaris contains wheat, clear water and nutrient solution；Nutrient solution contains the raw material of following weight percent meter It prepares：Glucose 66.18%-79.1%, protein adhesive 8.72%-13.08%, carbonic acid potassium dihydrogen 8.72%-13.08%, sulfuric acid Magnesium 3.42%-7.62%, vitamin B20.01%-0.02%, vitamin B120.01%-0.02%；By wheat and clear water according to Weight ratio 1:Nutrient solution is added in after (1.3-2.0) mixing, is stirred evenly in the basin body device for being fitted into and connecing Cordceps militaris strain, and Connect one polybag of set on the basin body device of Cordceps militaris strain；When carrying out high pressure sterilization, pressure is raised to 1.5 kilograms, and temperature is 120 DEG C -125 DEG C are kept for 30-40 minutes, are then removed stove, are started to cool down, exhaust is slowly opened when naturally ringing to 1 kilogram of pressure Valve drains into after pressure back to zero the 20 minutes horse backs that cool down and boils, is placed on after being cooled down in desinfection chamber and prepares inoculation；Transfer room is carried out to disappear Poison, not into progress Interior Space room disinfection before raw material, when using spray lysol 2 small first, every cubic metre of 15ml of lysol is watered 500ml, so The culture medium to have sterilized is moved into after being sterilized 30-60 minutes with 10A ozone machines afterwards；Material object disinfection, into before inoculation, in surge chamber Middle replacement work clothes, cap, mask, shoe cover take bacterium basin body again into transfer room with disinfectant wash clean, into transfer room after will Electronic inoculator starts, and alcohol is allowed to spray 2-3 minutes, pours out residual alcohol, then multiple with sterile water wash pipeline, adds after clean Enter strain.Cordceps militaris culturing room is moved into after finished in next step to be cultivated, completely black light culture must be kept within first 7 days；Disappear It is inoculated with after poison, bacterium solution need to be diluted with sterile water, and the ratio of bacterium solution and sterile water is 2:5；Culturing room sterilizes, and culturing room exists Thorough disinfection is carried out with ozone machine within 3-4 days before culture medium is moved into；Culture medium is moved into the training of thorough disinfection by cultural hypha Support room, be inoculated with bacterium basin discharge on the top of the shelf, the bacterium germination stage preceding 4-5 days indoor temperatures control 15-18 DEG C it Between, ventilation by forced draft 30 minutes is carried out daily；It can be appreciated that strain is sprouted after normal 2 days, cover within general 5-7 days, improve temperature adjustment To between 18-22 DEG C, humid control carries out ventilation by forced draft 2 times 30 minutes daily between 65-70%, indoor according to cultivating Actual conditions adjust；By the culture of 10-15 days, Chinese caterpillar fungus hypha covered with full basin.Give daily 20-24 it is small when illumination, pass through The scattering illumination of 4-5 days, when surface switchs to reddish yellow, mycelia nutrient growth is ripe；The management of flower bud and stroma, flower bud go out stroma In the process, daily scattering illumination be no less than 8 it is small when, at night also with solar radiation 16 it is small when；20-22 DEG C of indoor temperature, at night It is appropriate to reduce 5-8 DEG C；Indoor air humidity is controlled 70%；Ventilation 2-3 times daily, ventilation 20-40 minutes, treat mycelia every time Switched to by white after crocus and twist together into cima shape protuberance former base successively, when secreting yellow droplet, carry out out stroma management；Go out The humidity of stroma management, cordyceps sinensis go out before 6-7 days, room temperature control at 18-20 DEG C, later room temperature properly increases 2 DEG C, but cannot More than 25 DEG C；Sufficient scattering light, but it is unable to direct light photograph；Humidity is increased to 85%, treats that Cordceps militaris planar projection forms millet During grain former base, ventilation supplement fresh air；Cordyceps sinensis grow it is 2 centimeters long after, indoor humidity is increased to 90%, space wall water spray To improve humidity, strengthen ventilating management；After the long 3 centimeters of height of grass, illumination be reduced to daily 10-12 it is small when, when 6-4 is small, temperature difference 3- 5 degree, between humidity 85%-90%；Give within 3-5 days before harvesting 20 it is small when illumination Cordceps militaris color and luster it is good, ventilating management 50-60 days After can harvest.
Refer to the attached drawing 1-6, in view of Cordceps militaris belong in the aerobic mushroom of cryogenic thermostat type, in scarlet caterpiller fungus mycelium culture and flower bud and To the demand of epidemic disaster, illumination and gas during the management of stroma, the present invention realizes that Cordceps militaris potting fills in three-dimensional Industry Cultivation Put the following adaptability structure feature of tool：The setting of basin lid (2) bottom is horizontal along two (21), and horizontal set along two (21) is indulged along two (22), It is vertical that along two (22) upper ends, top layer (23) is set, groove (24) on top layer (23) is set, mounting ring (241) is set in groove (24), Mounting ring (241) one end is arranged on basin lid (2) outside, and mounting ring (241) other end is arranged in basin lid (2), mounting ring (241) Ventilating mechanism (3) is equipped with, in ventilating mechanism (3), mounting ring (241) lower end is equipped with air hole bottom cover (31), air hole bottom cover (31) ventilated membrane (32) is equipped between mounting ring (241), mounting ring (241) upper end is equipped with air hole upper cover (33), air hole Upper cover (33) side sets upper air hole (331), and ventilating mechanism (3) top and the bottom flush of basin body (1) are set；Secondly, Support ring (313) is set along base in air hole bottom cover (31), support ring (313) is adapted with mounting ring (241) and sets, and breathes freely Film (32) is arranged between support ring (313) and mounting ring (241), and support column (312), branch are set in air hole bottom cover (31) The height of dagger (312) is identical with the height of support ring (313), increases ventilative sky of the ventilated membrane (32) in air hole bottom cover (31) Between；Again, set between basin body (1) and basin lid (2) and increase layer (4), increased layer (4) upper end and raised one (42), protrusion one are set (42) set with vertical be adapted along two (22), increase layer (4) lower end and groove one (41) is set, groove one (41) and indulged along one (12) It is adapted and sets, groove one (41) and raised one (42) are adapted and set.
The present invention is adapted in the basin body of three-dimensional Industry Cultivation Cordceps militaris potting device, and the height for increasing layer (4) is 3cm- 10cm；
The present invention is adapted in the basin body of three-dimensional Industry Cultivation Cordceps militaris potting device, lower on air hole bottom cover (31) Along air hole bottom cover (31) geometric center, circumferentially array is set stomata (311), the upper air hole in air hole upper cover (33) (331) along air hole upper cover (33) axis, circumferentially array is set；
The present invention is adapted in the basin body of three-dimensional Industry Cultivation Cordceps militaris potting device, and the base of upper air hole (331) is higher than The upper surface of basin lid (2) is set, and ventilatory effect is good；
The present invention is adapted in the basin body of three-dimensional Industry Cultivation Cordceps militaris potting device, upper air hole (331) and lower air hole (311) quantity is 12；
The present invention is adapted in the basin body of three-dimensional Industry Cultivation Cordceps militaris potting device, and the surrounding of top layer (23) is respectively provided with recessed Slot (24).
The present invention is adapted in the basin body of three-dimensional Industry Cultivation Cordceps militaris potting device, and interior base (13) uses in basin body (1) Arc surface, convenient sterilizing and cleaning, basin body (1) and basin lid (2) use transparent material, facilitate daylighting.
When device stacked on top is placed, space is saved, top device basin body (1) bottom surface is with the multigroup ventilating machine of lower section device Structure (3) upper surface is as supporting point, while the gap between down devices so that air hole (331) ventilation is convenient and is not easy to fall Ash, single-row stack of device are placed, and daylighting is also enough, when cultivating the edible mushroom of different height, basin body (1) and basin lid (2) it Between different number be installed increase layer (4).
The above basin body technical characteristic for adapting to three-dimensional Industry Cultivation Cordceps militaris potting device, can ensure Cordceps militaris solid heap Interior environment carries out good cycling with culture room environmental in basin in folded cultivating process, and interior environment in basin is made to tend to culture room environmental Unanimously, ensure the demand of Cordceps militaris epidemic disaster and aerobic conditions, while can effectively prevent the intrusion of novel bacteria and dust, drop Low cultivation pollution rate ensures product quality.
In addition, the basin lid and basin body in the three-dimensional Industry Cultivation Cordceps militaris potting device of adaptation use transparent material, well Extraneous source stimulating is received, disclosure satisfy that Cordceps militaris during the management of cultural hypha and flower bud and stroma to scattering illumination Demand.
Embodiment seven：
Refer to the attached drawing 1-6, a kind of applicable Cordceps militaris potting stereoscopic cultivating device provided by the invention, including basin body (1) and Basin lid (2), basin body (1) top sets horizontal stroke, and along one (11), the horizontal setting along one (11) is vertical along one (12), vertical along one (12) and horizontal edge The orthogonal setting in one (11), basin lid (2) bottom sets horizontal stroke along two (21), horizontal vertical along two (22), vertical edge along the interior setting in two (21) Two (22) with horizontal along the orthogonal setting in two (21), it is horizontal to be set along one (11) and horizontal be adapted along two (21), indulge along one (12) with Vertical be adapted along two (22) is set, and indulges along two (22) upper ends and sets top layer (23), groove (24), groove are set on top layer (23) (24) mounting ring (241) is set in, and mounting ring (241) one end is arranged on basin lid (2) outside, and mounting ring (241) other end is arranged on In basin lid (2), mounting ring (241) is equipped with ventilating mechanism (3), and in ventilating mechanism (3), mounting ring (241) lower end is equipped with ventilative Bottom hole lid (31) is equipped with ventilated membrane (32) between air hole bottom cover (31) and mounting ring (241), mounting ring (241) upper end is equipped with Air hole upper cover (33), air hole upper cover (33) side set upper air hole (331), ventilating mechanism (3) top and basin body (1) Bottom flush is set.
The solid Industry Cultivation Cordceps militaris potting apparatus structure provided by the invention that adapts to is simple, easy to use, by using Basin body (1) adds culture medium and cultivates Cordceps militaris in factory, coordinates basin body by basin body (1) and the male and female button of basin lid (2) (1) and basin lid (2) effectively prevents extraneous air to be directly entered device, and basin lid (2) is equipped with ventilating mechanism, ventilating mechanism (3) top End is higher by the setting of basin lid (2) surface, and the equal flush in ventilating mechanism (3) top sets, put so that the solid heap of the present apparatus stacks It does not influence basin body (1) ventilation also, ensures the quality of culture Cordceps militaris, set on basin body (1) and increase layer (4), facilitate difference The cultivation of height Cordceps militaris, cultivating field in Cordceps militaris has wide applicability.
Embodiment eight：
It is provided by the invention to adapt in three-dimensional Industry Cultivation Cordceps militaris potting device, along base in air hole bottom cover (31) Support ring (313) is set, and support ring (313) is adapted with mounting ring (241) and sets, and ventilated membrane (32) is arranged on support ring (313) between mounting ring (241), support column (312), the height and branch of support column (312) are set in air hole bottom cover (31) The height of pushing out ring (313) is identical, increases ventilation space of the ventilated membrane (32) in air hole bottom cover (31).
It is provided by the invention to adapt in three-dimensional Industry Cultivation Cordceps militaris potting device, it is set between basin body (1) and basin lid (2) It puts and increases layer (4), increase layer (4) upper end and raised one (42) are set, raised one (42) and vertical be adapted along two (22) set, increase Layer (4) lower end sets groove one (41), and groove one (41) and vertical be adapted along one (12) are set, groove one (41) and protrusion one (42) it is adapted and sets.
Embodiment nine：
Provided by the invention to adapt in three-dimensional Industry Cultivation Cordceps militaris potting device, the height for increasing layer (4) is 3cm- 10cm。
It is provided by the invention to adapt in three-dimensional Industry Cultivation Cordceps militaris potting device, it is lower on air hole bottom cover (31) Along air hole bottom cover (31) geometric center, circumferentially array is set stomata (311), the upper air hole in air hole upper cover (33) (331) along air hole upper cover (33) axis, circumferentially array is set.
Provided by the invention to adapt in three-dimensional Industry Cultivation Cordceps militaris potting device, the base of upper air hole (331) is higher than The upper surface of basin lid (2) is set, and ventilatory effect is good.
It is provided by the invention to adapt in three-dimensional Industry Cultivation Cordceps militaris potting device, upper air hole (331) and lower air hole (311) quantity is 12.
Provided by the invention to adapt in three-dimensional Industry Cultivation Cordceps militaris potting device, the surrounding of top layer (23) is respectively provided with recessed Slot (24).
Provided by the invention to adapt in three-dimensional Industry Cultivation Cordceps militaris potting device, horizontal stroke sets through hole along one (11) (111), it is horizontal that the protrusion (211) being adapted with through hole (111) is set along two (21), the quantity of through hole (111) be it is more than two, Through hole (111) is arranged on the both sides of basin body (1), facilitates the folding of basin body (1) and basin lid (2).
Provided by the invention to adapt in three-dimensional Industry Cultivation Cordceps militaris potting device, interior base (13) uses in basin body (1) Arc surface, convenient sterilizing and cleaning, basin body (1) and basin lid (2) use transparent material, facilitate daylighting, basin body (1) use cube Body, cuboid, cylinder, Elliptic Cylinder shape.
Embodiment ten：
When being applicable in a kind of applicable Cordceps militaris potting stereoscopic cultivating device provided by the invention, operating personnel are in basin body (1) Cordyceps culturing medium is inside put into, cultivating Cordceps militaris under suitable culture environment in factory grows to a certain size, at this time basin body (1) Upper to cover basin lid (2), horizontal to ride over horizontal stroke along one (11) along two (21), vertical be buckled in along two (22) is indulged along one (12), forms male and female The cooperation of button, peasant or family of decentralized household can bring back device culture, and the culture medium placed in basin body (1) can be Cordceps militaris Nutrition is provided, external air due to horizontal stroke along one (11), it is vertical along one (12), it is horizontal can not along two (21) and the setting indulged along two (22) Enter from the junction of basin lid (2) and basin body (1), upper air hole (331) mistake that external air passes through air hole upper cover side Ventilated membrane (32) is interior into basin body (1), and effective blocks dust and external bacterium colony directly enter basin body (1), so as to ensure that Cordceps militaris Quality when device stacked on top is placed, saves space, top device basin body (1) bottom surface is with the multigroup ventilating mechanism of lower section device (3) Upper surface is as supporting point, while the gap between down devices so that air hole (331) ventilation is convenient and is not easy to fall ash, fills It puts single-row stack to place, daylighting is also enough, when cultivating the Cordceps militaris of different height, installs between basin body (1) and basin lid (2) Different number increases layer (4).
In the case where the Cordceps militaris potting solid Industry Cultivation of the present invention integrates cultivation system, operating personnel need to only put in basin body Enter cordyceps culturing medium, cordyceps sinensis is cultivated under suitable culture environment in factory after inoculation grows to a certain size, can be to peasant Or family of decentralized household is sold, it is not necessary to be sold again when Cordceps militaris maturation, this opens new for the popularization and application of Cordceps militaris Sales mode；Leap of the Cordceps militaris professional production to amateur production is realized simultaneously, allowing can to the interested citizen of Cordceps militaris Voluntarily to cultivate, and the growth overall process of Cordceps militaris is experienced, enjoy green, safe, delicious good product.
Embodiment 11：Potting solid Industry Cultivation integration system cultivates the sensory evaluation of Cordceps militaris
Cordceps militaris bacterium is connect to what the offer cordyceps militaris cultivation technique of embodiment nine and embodiment ten provided to above-described embodiment one On the basin body device basic of kind, sensory evaluation is carried out to the Cordceps militaris of cultivation, standard is shown in Table 1.
Table 1：Sensory evaluation scores standard (full marks 40 divide)
Note:Total score is the sum of each index scoring
Response phase method optimizes Cordceps militaris technique, and experimental factor is shown in Table 2 with level：
Table 2：Response surface experiments factor and water-glass
Table 3：Response surface experiments design and result
Response surface experiments design and result：
Bacterium germination phase temperature, strain sprout phase temperature during Cordceps militaris is cultivated, flower bud goes out stroma phase temperature, goes out stroma rank Duan Wendu is adapted with the structure of the basin body device provided by the invention for connecing Cordceps militaris strain and technical parameter.
Design of experiment analysis is carried out according to Box-Benhnken center combination designs principle, the result is shown in tables 3.Pass through DesignExpert8.0.6 is fitted the experimental data of table 3 using the method for polynomial fitting, obtains sensory evaluation scores R, northern worm The bacterium germination phase temperature (A) of grass, Cordceps militaris strain sprout phase temperature (B), Cordceps militaris flower bud goes out stroma phase temperature (C) and north The secondary multinomial regression model that cordyceps sinensis goes out stroma phase temperature (D) is：
R=37.00+0.17A+0.17B+0.083C-0.25D-1.25AD-2.58A2-2.83B2-3.21C2 -2.71D2。
Each factor reciprocation is referring specifically to attached drawing 7 to attached drawing 12 in response surface analysis.It can from the result of single factor experiment Know, the Cordceps militaris emphasis technological parameter cultivated under potting solid Industry Cultivation integration system is：Bacterium germination phase temperature is 15-18 DEG C, strain sprout that phase temperature is 18-22 DEG C, flower bud goes out that stroma phase temperature is 20-22 DEG C, to go out stroma phase temperature be 18- 20℃.It on above-mentioned experiment basis, is understood by further Optimal Experimental, Cordceps militaris bacterium germination phase temperature is 16.5 DEG C, northern worm Careless strain sprouts that phase temperature is 20 DEG C, Cordceps militaris flower bud goes out that stroma phase temperature is 21 DEG C, Cordceps militaris goes out stroma phase temperature For 19 DEG C.
The potting device of Cordceps militaris solid Industry Cultivation in Cordceps militaris potting solid Industry Cultivation is realized, effectively solves Floor space and moisturizing problem, meanwhile, it realizes three-dimensional culture by the way that benzvalene form is stackable, effectively solves pollution and floor space is asked The problem of inscribing, ensure Cordceps militaris quality, realizing extensive Cordceps militaris potting solid Industry Cultivation and factory culture.
In the case where the Cordceps militaris potting solid Industry Cultivation of the present invention integrates cultivation system, operating personnel need to only put in basin body Enter culture medium of Cordyceps militaris, cordyceps sinensis is cultivated under suitable culture environment in factory after inoculation grows to a certain size, can to peasant or Family of decentralized household is sold, it is not necessary to be sold again when cordyceps sinensis maturation, this opens new sale for the popularization and application of Cordceps militaris Pattern, while realize leap of the Cordceps militaris professional production to amateur production.Allowing can be certainly to the interested citizen of Cordceps militaris Row cultivation, and the growth overall process of Cordceps militaris is experienced, enjoy green, safe, delicious good product.
As described above, you can preferably realize the present invention, the above embodiments are only the side of being preferably implemented to the present invention Formula is described, and not the scope of the present invention is defined, and on the premise of design spirit of the present invention is not departed from, this field is general The various modifications and improvement that logical technical staff makes technical scheme, should all fall into present invention determine that protection domain It is interior.

Claims (10)

1. a kind of Cordceps militaris potting solid Industry Cultivation integration system, which is characterized in that integration system includes following technical step：
（1）Culture medium is prepared：Culture medium contains dry without the wheat, clear water and the nutrient solution that go mouldy；The weight ratio of wheat and water is 1:（1.3-2.0）；Nutrient solution contains glucose, protein adhesive, carbonic acid potassium dihydrogen, magnesium sulfate, vitamin B2, vitamin B12；
（2）Charging：Nutrient solution is added in after wheat is proportionally mixed with clear water, stirs evenly the basin for being packed into and connecing Cordceps militaris strain In body device, and a polybag is covered on the basin body device for connect Cordceps militaris strain；
（3）High pressure sterilization：High pressure sterilization is carried out, is placed on after being cooled down in desinfection chamber and prepares inoculation；
（4）Transfer room sterilizes：Indoor vacant room disinfection and disinfection in kind, are inoculated with, bacterium solution need to be carried out dilute with sterile water after disinfection It releases, the weight ratio of bacterium solution and sterile water is 2:5；
（5）Culturing room sterilizes：Culturing room's carrying out disinfection for 3-4 days before culture medium is moved into；
（6）Cultural hypha：Culture medium is moved into the culturing room of thorough disinfection, the strain discharge being inoculated on the top of the shelf, In the bacterium germination stage into trip temperature and the adjusting of illumination；
（7）The management of flower bud and fructification：Flower bud goes out during stroma, control scattering illumination and night light application time, Indoor Temperature, Humidity and the ventilation frequency.
2. a kind of Cordceps militaris potting solid Industry Cultivation integration system according to claim 1, which is characterized in that described During culture medium is prepared, each material component of nutrient solution matches by weight percentage is：Glucose 66.18%-79.1%, protein adhesive 8.72%-13.08%, carbonic acid potassium dihydrogen 8.72%-13.08%, magnesium sulfate 3.42%-7.62%, vitamin B20.01%-0.02%, dimension Raw element B120.01%-0.02%。
A kind of 3. Cordceps militaris potting solid Industry Cultivation integration system according to claim 2, which is characterized in that the training In the basigamy system of supporting, each material component of nutrient solution matches by weight percentage is：Glucose 72.64%, protein adhesive 10.90%, carbon Acid dihydride potassium 10.90%, magnesium sulfate 5.52%, vitamin B20.02%th, vitamin B120.02%。
4. a kind of Cordceps militaris potting solid Industry Cultivation integration system according to claim 1, which is characterized in that described Step（3）Mesohigh should empty cold air when sterilizing, pressure starts to calculate sterilization time, thermometer 120- when being raised to 1.5 kilograms Stove is removed after being kept for 30-40 minutes at 125 degree, starts to cool down, naturally rings to slowly open air bleeding valve during 1 kilogram of pressure, is arranged Boil at once after to pressure back to zero, to be opened slowly when boiling it is small open, at least cool down takes the dish out of the pot for 20 minutes, is placed on accurate after being cooled down in desinfection chamber Standby inoculation；
The step（4）Transfer room disinfection is divided into indoor vacant room disinfection and disinfection in kind：3*5 squares of the careat of transfer room Rice, sealed around, the door of transfer room must have surge chamber；It is small with spray lysol 2 first not into progress Interior Space room disinfection before raw material When, every cubic metre of 15ml of lysol is watered 500ml, and the culture to have sterilized is moved into after then being sterilized 30-60 minutes with 10A ozone machines Base；Material object disinfection, into before inoculation, replaces work clothes in surge chamber, cap, mask, and shoe cover washes bacterium basin body with disinfectant Totally taken again into transfer room, into transfer room after electronic inoculator is started, allow alcohol spray 2-3 minutes, pour out residual alcohol, It is multiple with sterile water wash pipeline again, add in strain after clean；The culture of Cordceps militaris culturing room is moved into after finished in next step to carry out Culture, must keep completely black light culture in first 7 days；
The step（5）Culturing room need to be before culture medium be moved into carry out thorough disinfection with ozone machine within 3-4 days；
The step（6）Cultural hypha the bacterium germination stage preceding 4-5 days indoor temperatures control between 15-18 DEG C, daily into Row ventilation by forced draft 30 minutes；It can be appreciated that strain is sprouted after 2 days, cover within 5-7 days bacterium, improve temperature and be adjusted between 18-22 DEG C, it is wet Degree control carries out ventilation by forced draft 2 times 30 minutes daily between 65-70%, is adjusted according to indoor actual conditions are cultivated；By The culture of 10-15 days, Chinese caterpillar fungus hypha cover with full basin；Give daily 20-24 it is small when illumination, by the scattering illumination of 4-5 days, table When face switchs to reddish yellow, mycelia nutrient growth is ripe；
The step（7）Flower bud goes out during stroma, daily scatter illumination be no less than 8 it is small when, at night also use solar radiation 16 it is small when；20-22 DEG C of indoor temperature is appropriate at night to reduce 5-8 DEG C；Indoor air humidity is controlled 70%；Ventilation 2-3 times daily, Ventilation 20-40 minutes every time twist together into after mycelia switchs to crocus by white and successively cima shape protuberance former base, secrete yellow During droplet, you can carry out the preparation of long stroma of Cordyceps militaris；Go out the humidity of stroma management, cordyceps sinensis go out before 6-7 days, room temperature control For system at 18-20 DEG C, later room temperature improves 2 DEG C, but no more than 25 DEG C；Sufficient scattering light, but it is unable to direct light photograph；Humidity carries Height is to 85%, when Cordceps militaris planar projection forms small rice grain former base, ventilation supplement fresh air；Cordyceps sinensis grow it is 2 centimeters long after, Indoor humidity is increased to 90%, wall water spray in space strengthens ventilating management to improve humidity；After the long 3 centimeters of height of grass, illumination can Be reduced to daily 10-12 it is small when, when 6-4 is small, temperature difference 3-5 degree, between humidity 85%-90%；Gave within 3-5 days before harvesting for 20 small time According to Cordceps militaris color and luster it is good, ventilating management can harvest after 50-60 days.
5. a kind of Cordceps militaris potting solid Industry Cultivation integration system according to claim 1, which is characterized in that described The basin body device of Cordceps militaris strain is connect, including basin body and basin lid, which is characterized in that basin body top sets horizontal stroke, and horizontal stroke is along one along one Set it is vertical along one, indulge along one with horizontal along an orthogonal setting, basin lid bottom set it is horizontal along two, it is horizontal set along two it is vertical along two, It is vertical along two with horizontal along two orthogonal settings, it is horizontal to be set along one and horizontal be adapted along two, indulge and set along one and vertical be adapted along two, It is vertical that along two upper ends, top layer is set, groove on top layer is set, mounting ring is set in groove, mounting ring one end is arranged on outside basin lid, is pacified The dress ring other end is arranged in basin lid, and mounting ring is equipped with ventilating mechanism, and in ventilating mechanism, mounting ring lower end is equipped with air hole bottom Lid is equipped with ventilated membrane between air hole bottom cover and mounting ring, mounting ring upper end is equipped with air hole upper cover, and air hole upper cover side is set Put air hole, the bottom flush setting of ventilating mechanism top and basin body.
6. a kind of Cordceps militaris potting solid Industry Cultivation integration system according to claim 5, which is characterized in that described It connects in the basin body device of Cordceps militaris strain, support ring is set along base using in air hole bottom cover, support ring is mutually fitted with mounting ring It should set, ventilated membrane is arranged between support ring and mounting ring, and support column, the height and branch of support column are set in air hole bottom cover The height of pushing out ring is identical；It is set using the base of upper air hole higher than the upper surface of basin lid.
7. a kind of Cordceps militaris potting solid Industry Cultivation integration system according to claim 5, which is characterized in that described It connects in the basin body device of Cordceps militaris strain, increases layer using being set between basin body and basin lid, increase layer upper end and protrusion one is set, it is convex It plays one to set with vertical be adapted along two, increases layer lower end and groove one is set, groove one is set with vertical be adapted along one, one He of groove Protrusion one, which is adapted, to be set；Groove is respectively provided with using the surrounding of top layer；Through hole is set along one using horizontal stroke, horizontal stroke set along two with The protrusion that through hole is adapted, the quantity of through hole is more than two, and through hole is arranged on the both sides of basin body.
8. a kind of Cordceps militaris potting solid Industry Cultivation integration system according to claim 5, which is characterized in that described Connect in the basin body device of Cordceps militaris strain, using the lower air hole on air hole bottom cover along air hole bottom cover geometric center circumferentially Array is set, and along air hole upper cover axis, circumferentially array is set the upper air hole on air hole on lid.
9. a kind of Cordceps militaris potting solid Industry Cultivation integration system according to claim 5, which is characterized in that described It connects in the basin body device of Cordceps militaris strain, uses and increase the height of layer as 3cm-10cm；The upper air hole and lower air hole Quantity be 12.
10. a kind of Cordceps militaris potting solid Industry Cultivation integration system according to claim 5, which is characterized in that described Connect in the basin body device of Cordceps militaris strain, using interior base in basin body using arc surface, basin body and basin lid using transparent material, Basin body is using cube, cuboid, cylinder, Elliptic Cylinder shape.
CN201711160993.9A 2017-11-20 2017-11-20 A kind of Cordceps militaris potting solid Industry Cultivation integration system and application Pending CN108040740A (en)

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CN114375771A (en) * 2020-10-02 2022-04-22 湖南金芙农业科技有限公司 Watering device of usefulness is planted to cordyceps militaris

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CN114375771A (en) * 2020-10-02 2022-04-22 湖南金芙农业科技有限公司 Watering device of usefulness is planted to cordyceps militaris

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