CN101874459B - Method for controlling root knot nematode by utilizing ozone water - Google Patents
Method for controlling root knot nematode by utilizing ozone water Download PDFInfo
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- CN101874459B CN101874459B CN 200910020765 CN200910020765A CN101874459B CN 101874459 B CN101874459 B CN 101874459B CN 200910020765 CN200910020765 CN 200910020765 CN 200910020765 A CN200910020765 A CN 200910020765A CN 101874459 B CN101874459 B CN 101874459B
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- ozone water
- root
- knot nematode
- plant
- ozone
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- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 title claims abstract description 54
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 49
- 241000243785 Meloidogyne javanica Species 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 17
- 241000196324 Embryophyta Species 0.000 claims abstract description 25
- 230000008635 plant growth Effects 0.000 claims abstract description 6
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 5
- 238000003973 irrigation Methods 0.000 claims abstract 3
- 230000002262 irrigation Effects 0.000 claims abstract 3
- 201000010099 disease Diseases 0.000 claims description 14
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 15
- 238000005516 engineering process Methods 0.000 abstract description 4
- 230000006378 damage Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 235000013311 vegetables Nutrition 0.000 description 6
- 241000227653 Lycopersicon Species 0.000 description 5
- 241000244206 Nematoda Species 0.000 description 5
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
- 241000219109 Citrullus Species 0.000 description 3
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 210000004681 ovum Anatomy 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 238000005562 fading Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001143352 Meloidogyne Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 238000003967 crop rotation Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
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- 229920002477 rna polymer Polymers 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses a method for controlling root knot nematode by utilizing ozone water. In the method, the ozone water is used as root irrigation physic liquor, and a plant root zone is irrigated once per 5-15 days until fruits are harvested. By utilizing the ozone water to continuously carry out root irrigation on plant soils for many times, the generation of the root knot nematode can be efficiently controlled, damage on the growth of plants can not be caused either, and the method is a new technology of green food production.
Description
Technical field
The invention belongs to plant root-knot nematodes Prevention Technique field, especially relate to a kind of method of utilizing Ozone Water control plant root-knot nematodes.
Background technology
Plant root-knot nematodes is the infectious disease that is caused by nematode, occurs quite generally in China, and harm is serious, and its host plant has 114 sections kind more than 3000.At vegetables production area, along with year after year continuous cropping plantation, the incidence of disease rises year by year.According to investigations, the booth vegetable root knot nematode disease incidence of disease is generally at 30%-50% at present, and indivedual areas are about 70%, and serious reaches 100%.In a single day nematodosis occur in booth vegetable, the lighter's underproduction 20%-30%, severe one underproduction 50%-70%, even total crop failure.Root knot nematode disease has become the major obstacles that protection ground pollution-free vegetable produces.
The root-knot nematode main activities is under the soil table in the 20-30cm soil layer.Under field conditions (factors), the root nematode can breed for 5 to 10 generations every year, and can breed in all seasons in green house, repeatedly infected danger to vegetables.The root knot nematode disease method of preventing and treating mainly contains high temperature and kills method, crop rotation, soil disinfection and chemical control etc.Before front 3 kinds of methods were mainly used in doing plant setting, not thorough owing to killing, crop growth period nematodosis recurrence rate was high.Chemical control mainly contains fumigating system and non-fumigating system, because the root-knot nematode main activities is under the soil table, the fumigating system effect is difficult to play a role, and non-fumigating system uses agricultural chemicals to kill in a large number, and is seriously polluted.
The certain density Ozone Water of sprinkling in canopy that also has is killed bacterium, virus, fungi and worm's ovum.But because the root-knot nematode main activities is in the rhizosphere soil layer, and Ozone Water very easily from water, overflowing and voluntarily decomposition in water, this method does not only have insecticidal action, and can cause certain damage to the crop acrial part, simultaneously, the ozone of soil middle and high concentration has inhibitory action to plant growth, therefore, and so far there are no the report that utilizes Ozone Water control subterraneous root Root-knot.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of method of utilizing Ozone Water control plant root-knot nematodes.
For solving the problems of the technologies described above, the present invention utilizes concentration plant root district to be irrigated once every 5-15 days after the plant field planting for the Ozone Water of 1.3-3.3ppm, until fruit harvest take Ozone Water as filling with the root liquid.
Consistency of ozone water is along with the growth of plant raises gradually during pouring.
Filling with the root mode is to irrigate once with the 1.7-3.0ppm Ozone Water every 7-12 days after the plant field planting.
The invention has the beneficial effects as follows: ozone can destroy the cell nucleus ribosomal ribonucleic acid as a kind of strong oxidizer, decomposes the macromolecule polyalcohols such as DNA (deoxyribonucleic acid), protein, lipid and polysaccharide, plays sterilization and presses down the worm effect.Utilize Ozone Water to irrigate plant root district, Ozone Water can be infiltrated in the soil layer fast, and decompose rapidly, generation has the sub-oxygen in unit of strong oxidation, the organic substances such as bacterium, microorganism, worm's ovum in the soil are decomposed, thereby suppress and kill root knot line larva or ovum, the control root knot nematode disease occurs.Its sterilization of certain density Ozone Water can be finished in moment, can be reduced to oxygen after half an hour in soil, not only can not work the mischief to plant root, and can improve soil environment, played the effect that promotes plant strain growth.The vegetables in greenhouse booth evidence is processed root-knot nematode lesion soil with Ozone Water, fill with root 3-4 time after, the minimizing of root-knot nematode 2 instar larvae quantity contrasts is processed 10 times more than 30% continuously in the treatment region soil, contrast reduces more than 60%; After the booth of being injured was processed, crop not only output was significantly improved, and quality obtained larger improvement, had both solved the difficult problem of root knot nematode disease control, had reduced again the impact of ozone on plant growth, was a kind of new technology of pollution-free food production.
The invention will be further described below in conjunction with embodiment:
Embodiment 1
Ozone Water is irrigated tomato in plastic greenhouse, utilize air-cooled discharging ozone generator to produce ozone, the ozone that produces directly passes in the aqueduct blender, make it fully soluble in water, ozone concentration is controlled at 1.3-1.7ppm, Ozone Water is piped to the tomato root district and irrigates, after definite value, watered once every 10-15 days, water according to its daily growth needs, every minor tick 12-15 days early stage was with the pouring of 1.3-1.7ppm Ozone Water, every minor tick 10-12 of later stage days, with the pouring of 1.5-1.7ppm Ozone Water, water altogether 10 to 12 times; After the use, the tomato root-knot eelworm disease is effectively suppressed, and only less than 5% plant root a small amount of root nodule is arranged, and vegetative period, tomato plant strain growth was normal, does not have the plant of being fading to occur.
Embodiment 2
Ozone Water is irrigated Melon, utilize air-cooled discharging ozone generator to produce ozone, the ozone that produces directly passes in the aqueduct blender, make it fully soluble in water, ozone concentration is controlled at the 1.7-3.0ppm Ozone Water to be piped to plant root district and to irrigate, according to the plant strain growth needs, watered once every 10-12 days after the definite value, water 8-10 time altogether; Early stage, every minor tick was 12 days, with the pouring of 1.7-2.0ppm Ozone Water, and every minor tick 10-11 of later stage days, with the pouring of 2.0-3.0ppm Ozone Water; After the use, plant generally grows vigorous, and the leaf look dark green, all plant root-knot nematode disease symptoms such as do not occur being fading, and fruit growth is normal, only have 10% plant root that root nodule is arranged, but quantity is few, and volume is little.
Embodiment 3
Ozone Water is irrigated greenhouse watermelon, and ozone concentration is controlled at 2.1-3.3ppm, waters once every 5-10 days after the definite value, every minor tick 8-10 days early stage was with the pouring of 2.1-3.0ppm Ozone Water, every minor tick 5-7 of later stage days, water with the 3.0-3.3ppm Ozone Water, water 7-9 time, also can determine pouring number of times and pouring concentration according to growing state, the sick incidence of watermelon root-knot is below 5%, there is 10% plant root that a small amount of knurl is arranged, but do not have manifest symptom, watermelon fruit shape just, quality better.
Tomato in plastic greenhouse meloidogyne 2 instar larvae testing results (subordinate list)
Detection method: to the abundant mixing of each sample soil sample, respectively get 1000g, after nematode separates, kills and fixes, count.It can be seen from the table, process the larva mean with the contrast soil sample, detect for the first time (before processing) quantity and be respectively 49.2 and 53, detect for the second time (after processing) quantity and be respectively 54 and 80.6, detect for the third time (after processing) quantity and be respectively 89.44 and 265.Can find out untreated control group, along with the growth of plant, nematode has increased by 400%, and the processed group nematode has increased by 80%, and the Ozone Water root-knot nematode procreation of having processed establishment has been described.
Subordinate list
Claims (4)
1. method of utilizing Ozone Water to prevent and treat root knot nematode disease, comprise the medicine liquid irrigation root, it is characterized in that described liquid is Ozone Water, utilize concentration plant root district to be irrigated once every 5-15 days after the plant field planting for the Ozone Water of 1.3-3.3ppm, until fruit harvest.
2. according to the method for utilizing Ozone Water to prevent and treat root knot nematode disease claimed in claim 1, consistency of ozone water is along with the growth of plant raises gradually when it is characterized in that watering.
3. according to the method for utilizing Ozone Water to prevent and treat root knot nematode disease claimed in claim 2, it is characterized in that filling with the root mode is to irrigate once with the 1.7-3.0ppm Ozone Water every 7-12 days after the plant field planting.
4. according to the method for utilizing Ozone Water to prevent and treat root knot nematode disease claimed in claim 2, it is characterized in that described Ozone Water produces ozone by the ozone generator scene and directly pass into aqueduct miscible and get, Ozone Water is through the pipe pressurized irrigated area that is delivered to.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910020765 CN101874459B (en) | 2009-04-30 | 2009-04-30 | Method for controlling root knot nematode by utilizing ozone water |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910020765 CN101874459B (en) | 2009-04-30 | 2009-04-30 | Method for controlling root knot nematode by utilizing ozone water |
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| CN101874459A CN101874459A (en) | 2010-11-03 |
| CN101874459B true CN101874459B (en) | 2013-02-13 |
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| CN 200910020765 Expired - Fee Related CN101874459B (en) | 2009-04-30 | 2009-04-30 | Method for controlling root knot nematode by utilizing ozone water |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004535A (en) * | 2012-12-28 | 2013-04-03 | 上海拜森生物技术有限公司 | Method for carrying out crop pest control and prevention by applying ozone water |
| CN103636439B (en) * | 2013-10-27 | 2015-12-09 | 大连东芳果菜专业合作社 | Greenhouse fruit and vegetable ozone fumigation control disease technical method |
| CN104396670A (en) * | 2014-11-20 | 2015-03-11 | 江苏省农业科学院 | Method for improving ornamental effect of Jinling Huangfeng in autumn |
| CN107750557A (en) * | 2017-10-19 | 2018-03-06 | 青岛农业大学 | A kind of Ozone Water application process for plastic tent cucumber |
| CN107637244A (en) * | 2017-10-19 | 2018-01-30 | 青岛农业大学 | A kind of leek pours the application process of microelement-supplementing after Ozone Water |
| CN108064525A (en) * | 2017-12-08 | 2018-05-25 | 青岛农业大学 | The method of administration of microelement-supplementing after Ozone Water is poured and sprayed to a kind of spinach |
| CN108093726A (en) * | 2017-12-08 | 2018-06-01 | 青岛农业大学 | A kind of method that soil pours extra-nutrition substance after Ozone Water |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH06157229A (en) * | 1991-03-25 | 1994-06-03 | Yamamoto Sangyo:Kk | Production of organism controlling material |
| CN1274220C (en) * | 2004-03-05 | 2006-09-13 | 中国农业大学 | Method for preventing and controlling soil-transmitted diseases to plants |
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