CN101999416A - Nematode controlling reagent and production flow thereof - Google Patents
Nematode controlling reagent and production flow thereof Download PDFInfo
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- CN101999416A CN101999416A CN2010105732436A CN201010573243A CN101999416A CN 101999416 A CN101999416 A CN 101999416A CN 2010105732436 A CN2010105732436 A CN 2010105732436A CN 201010573243 A CN201010573243 A CN 201010573243A CN 101999416 A CN101999416 A CN 101999416A
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Abstract
The invention discloses a nematode controlling reagent which comprises bacteria genus which predominantly is actinomycete and bacillus, metabolites generated in the process of vital activities of the bacteria genus, sun euphorbia as a traditional Chinese medicine which has the killing action on nematode adults and abamectin as a biological insecticide. The nematode controlling reagent is prepared from a medium containing 2.0 hundred million actinomycete, bacillus and trichoderma per gram, 2 percent abamectin oil and the sun euphorbia according to the ratio of 10:1:1. The invention is superior to other pesticides on the effect of controlling nematode on vegetables in a protective ground, can also promote growth of a plant root system and has certain control effects on common soil-borne diseases such as blight, root rot, verticillium wilt and basal stalk rot of the vegetables in the protective ground.
Description
Technical field
The present invention relates to a kind of technical field of biocide, be specifically related to a kind of nematoda control medicament and production procedure thereof.
Background technology
Protection ground root knot nematode took place and caused harm to present the trend of cumulative year after year in recent years; the generation of nematodosis and causing harm; cause protection ground vegetables particularly the solanberry class and melon etc. occur that output reduces, product qualitative change is bad, a little less than the plant strain growth gesture and the early ageing phenomenon very easily occurs; the gently then underproduction, fall ill serious plot even total crop failure.The vegetable grower prevents and treats root-knot nematode and usually adopts hypertoxic high residue such as insecticides such as legendary god of farming's pellet, phonamiphos, has not only polluted environment, and is unfavorable for the vegetables green non-pollution production of protection ground.The long-term use of these highly toxic pesticide is developed immunity to drugs a lot of regional root-knot nematodes simultaneously, and the peasant has to take to increase the measures such as usage amount of agricultural chemicals.
A lot of at present vegetable growers in the localities are in order to prevent and treat root knot nematode, the severe toxicity residual life long insecticides of adopting as legendary god of farming's pellet, phonamiphos etc. more, severe contamination environment, and be unfavorable for the safety in production of pollution-free vegetable, though import agricultural chemicals such as happy lot are many to vegetables and environmental pollution is less but not only consumption is big, cost is high, the term of validity is shorter after the dispenser.One big batch of crop such as grafting cucumber, it is not only time-consuming to have to carry out the control second time spring in next year, and has increased production cost.
Along with the raising of people's living standard, the requirement of food quality is more and more stricter, not only will have enough and more will eat.Victual with residue of pesticide does not more and more have market.The use of severe toxicity persistent pesticide has limited the protection vegetables and has produced healthy and orderly development.The insecticide of development high-efficiency low-toxicity noresidue control nematode is not only the needs of evading widespread pollution from the overuse of fertilizers and pesticides in rural area, and is an urgent demand of the nuisanceless safety in production of vegetables.Environment has been polluted in the use of highly toxic pesticide, has caused agricultural residual exceeding standard simultaneously, is unfavorable for national health, has limited protection ground vegetables simultaneously and has further developed.Import pesticide price height, vegetable grower's production cost is big, and victual lacks market competition advantage.
The Chinese patent publication number is CN101480181, the Invention Announce specification that open day is on July 15th, 2009 discloses " a kind of pesticide capsule preparation of preventing and treating nematode, soil-borne disease ", this invention relates to soil fumigant 1,3-dichloropropylene (general by name 1,3-dichloropropene, chemical formula C3H4Cl2) the capsule preparations machining manufacture.This compound is applicable to root-knot nematode and other soil nematodes on the crops such as control vegetables, strawberry, flowers, melon, asparagus, celery, carrot, sweet potato, soybean, orchard, vineyard, Chinese herbal medicine, and this compound has certain preventive effect to soil-borne pathogen Fusarium (Fusarium spp.) and phytophthora genus soil-borne pathogens such as (Phytophthora spp.).But this invention still belongs to the pesticidal preparations that has toxicity, does not avoid pollution or influence to environment, and the effect of not improving the soil.
Summary of the invention
The objective of the invention is at deficiencies such as pesticidal preparations contaminated environment, the control efficiency of existing control nematode are bad, a kind of avoid contaminated environment, nematoda control medicament and production procedure thereof that control efficiency is outstanding are provided.
The objective of the invention is to realize: a kind of nematoda control medicament by following technological means, include: actinomycetes, bacillus are master's Pseudomonas and the metabolite that they are produced in vital movement, are equipped with the Chinese medicine wartwort and the biological insecticides Avermectin that the nematode adult there are killing action; Contain 2.0 hundred million actinomycetes, bacillus, Trichoderma as medium with every gram: 2% abamectin emulsifiable concentrate: wartwort is that 10: 1: 1 ratio proportioning is made.
Described Chinese medicine wartwort comprises wartwort saponin(e (phasin), Quercetin-3,5-digalactosyl glycosides (tithymalin), dihydrofucosterol (β-dihydrofucosterol), helioscopiol (hellscopiol), butyric acid and calcium malate.
Described bacillus can replace with bacillus subtilis, and wartwort can be with replacements such as sweet-scented oleanders, and 2% abamectin emulsifiable concentrate can replace with 1.8% abamectin emulsifiable concentrate.
A kind of production procedure of nematoda control medicament is characterized in that: (1) actinomycetes, bacillus are that main Pseudomonas carries out the viable bacteria cultivation, soaks activated viable bacteria in 3 hours after, it is standby that the interpolation peat composed of rotten mosses is made raw material 1; (2) after wartwort is gathered, pulverize, sieve, it is standby to make raw material 2; (3) 2% abamectin emulsifiable concentrates add organosilicon, and it is standby to make raw material 3; (4) with raw material 1: raw material 2: raw material 3 fully mixed at the shady place below 15 ℃ by 10: 1: 1, promptly got this product.
Compared with prior art the present invention has following tangible advantage: (1) the present invention kills adult with the bacterium killing ova with biopesticide, realizes that worm's ovum is double to control, and it is remarkable that soil such as root-knot nematode, packing nematode, stem eelworm are passed parasitic control efficiency.(2) the present invention can improve the soil, increase soil group and prevents to harden, improve organic content, molten phosphorus potassium decomposing fixed nitrogen, replenish crop alimentary comprehensively, use the thing disease of getting off quickly, recover growth, balance soil bacterium colony of the present invention comprehensively, prevent plant from poison, for root system of plant is created good growing environment.(3) environmentally safe of the present invention, to people and animals' low toxicity, to the vegetables noresidue, evaded and used of the pollution of hypertoxic high residue insecticide environment and victual.The present invention prevents and treats protection ground vegetables nematode effect and all is better than other agricultural chemicals generally, can promote the plant root growth simultaneously, and the common soil-borne disease of protection ground vegetables such as fusarium wilt, root rot, verticillium wilt, base rot disease are all had certain preventive effect.
Description of drawings
Fig. 1 is a flow sheet of the present invention;
Embodiment
The present invention is described in further detail below in conjunction with embodiment:
A kind of nematoda control medicament includes: actinomycetes, bacillus are master's Pseudomonas and the metabolite that they are produced in vital movement, are equipped with the Chinese medicine wartwort and the biological insecticides Avermectin that the nematode adult there are killing action; Contain 2.0 hundred million actinomycetes, bacillus, Trichoderma as medium with every gram: 2% abamectin emulsifiable concentrate: wartwort is that 10: 1: 1 ratio proportioning is made.Described Chinese medicine wartwort comprises wartwort saponin(e (phasin), Quercetin-3,5-digalactosyl glycosides (tithymalin), dihydrofucosterol (β-dihydrofucosterol), helioscopiol (hellscopiol), butyric acid and calcium malate.
This nematoda control medicament can adopt other biologic live bacteria as: bacillus licheniformis, bacillus subtilis, Paecilomyces lilacinus bacterium, heavy wall Verticillium bacterium etc. the preparation the nematoda control preparation, perhaps use preparations such as biopesticide such as Avermectin to substitute merely.For example, described bacillus can replace with bacillus subtilis, and wartwort can be with replacements such as sweet-scented oleanders, and 2% abamectin emulsifiable concentrate can replace with 1.8% abamectin emulsifiable concentrate.
This nematoda control medicament mainly is raw material with the biologic live bacteria, be equipped with biology and Pesticide with Chinese medicinal material, reach the adult of killing root-knot nematode and the purpose of worm's ovum, because biologic live bacteria is subjected to natural environment influence bigger, therefore need relatively strict storage condition, take to re-use behind the activation viable bacteria in 3 hours, use little convenience with respect to cream preparation towards soaking when executing, but capsule preparations is easy to use, and cost is relative higher with other control nematode medicament.
A kind of production procedure of nematoda control medicament is characterized in that: (1) actinomycetes, bacillus are that main Pseudomonas carries out the viable bacteria cultivation, soaks activated viable bacteria in 3 hours after, it is standby that the interpolation peat composed of rotten mosses is made raw material 1; (2) after wartwort is gathered, pulverize, sieve, it is standby to make raw material 2; (3) 2% abamectin emulsifiable concentrates add organosilicon, and it is standby to make raw material 3; (4) with raw material 1: raw material 2: raw material 3 fully mixed at the shady place below 15 ℃ by 10: 1: 1, promptly got this product.
This nematoda control medicament has two kinds of preparations, and one pulvis, said preparation have three kinds of occupation modes: base is executed, spreads manuer in holes, is dashed and execute.Its two capsule uses the capsule human body directly not contact medicine, and is as safe as a house to human body, spreads manuer in holes during field planting.The present invention prevents and treats and protects ground vegetables nematode effect all to be better than other agricultural chemicals generally, said preparation is not only held concurrently to the root-knot nematode worm's ovum and is killed, and balance the soil bacterium colony, to the common soil-borne disease of protection ground vegetables as: fusarium wilt, root rot, verticillium wilt, base rot disease all have certain preventive effect, can promote the plant root growth simultaneously; Crop early ageing phenomenon had certain prevention effect.
2008; 2009; 2010 first half of the year are continuously at Zhou Cheng, Shandong Province protection purpleflower violet herb; protection ground tomato; the open country celery; having carried out base on the open country cauliflower executes; spread manuer in holes; dash the test of different administration modes such as executing; simultaneously with highly toxic pesticide legendary god of farming pellet; phonamiphos; biological pesticide avermectin; import agricultural chemicals happy lot waits insecticide to carry out comparative trial more; the result shows: this nematoda control chemical control protection ground vegetables nematode effect all is better than other agricultural chemicals; can promote simultaneously the plant root growth, to common soil-borne disease such as the fusarium wilt of protection ground vegetables; root rot; verticillium wilt; base rot disease all has certain preventive effect.
The application test of nematoda control medicament of the present invention on protection ground tomato
Root knot nematode disease is one of main disease of protection ground tomato; protection ground tomato whole growing is caused harm by root-knot nematode all can; the disease plant poor growth; even stop growing; cause harm when serious; the empty fruit of empty bar appears; cause to drop in production over a large area, marketing quality descends, and control root-knot nematode vegetable grower usually uses highly toxic pesticide; the use of highly toxic pesticide; be unfavorable for the production of pollution-free vegetable, nematoda control medicament of the present invention is according to the nematode life habit; characteristics cause harm; with nuisanceless; noresidue is an aim, and the desinsection of research and development kills the ovum medicament, in order to verify the result; 2008; on greenhouse by solar heat autumn delay tomato, carried out the pharmacy effect test, and now be summarized as follows in 2009 continuous 2 years:
1 materials and methods
1.1 reagent agent
The red granule (production of Shandong Province Hua Yang insecticide factory) of 5% legendary god of farming; 10% chlopyrifos granule (Zhejiang Xinan Chemical Industry Group Co.Ltd's production); (annotate: these two kinds of medicines belong to highly toxic pesticide, and national strictness is limited on the vegetables to be used, and wash down effect in order to verify nematode one, just as a comparison, test.); 2% abamectin emulsifiable concentrate (section's green bio Engineering Co., Ltd produces in the Jinan); The many granules of 10% happy lot (production of the former industry stone of Japanese strain formula commercial firm); Nematoda control medicament of the present invention (the innoxious nematoda control medicaments of research and development); The ck clear water in contrast.
1.2 tomato variety
Tomato variety is: golden canopy complete victory.
1.3 controlling object
Protection ground tomato root-knot eelworm disease (Tomato root-knot nematode disease)
1.4 crop genetics and environment
Experimental field being located at Su Zhendong Gu Cun hole, north, Zoucheng City, Shandong Province ties up in the magnificent greenhouse by solar heat, it experimental field is cinnamon soil, autumn postpones tomato and grows seedlings mid-August, late September, field planting was in greenhouse by solar heat, ridge culture, two strains on the ridge, 33 centimetres of spacing in the rows, the size row spacing is 50cm * 70cm, and other postpones the tomato normal management with the daylight greenhouse autumn.
1.5 experimental scheme
Test when the tomato field planting and carry out the sub-district area: the wide 0.5m of long 8m, area 4.0m
2, 48 strains are planted in every sub-district, 3 repetitions, and district's group is arranged at random, and test is handled and is seen table one for details, according to the every 667m of table one
2Institute's dosage calculates each sub-district different disposal agricultural chemicals usage amount, and each processing all when the tomato field planting, applies in the planting pit.
Table one test is handled
1.6 investigation content and statistical method
Late December is carried out each effect of chemical control when tomato is gathered in the crops, and every sub-district is got 4 points at random, looks into 2 strains at every, 15 roots of every strain random searching.Grade scale (is unit with the root): 0 grade of root system does not produce fertile swollen lopsided warty spot; 1 grade: fertile swollen lopsided warty spot accounts for below 5% of whole root system; 3 grades: fertile swollen lopsided warty spot accounts for the 6%-10% of whole root system; 5 grades: fertile swollen lopsided warty spot accounts for the 11%-25% of whole root system; 7 grades: fertile swollen lopsided warty spot accounts for the 26%-50% of whole root system.
2 results and analysis
5 kinds of insecticides of table two are to the sick control efficiency of protection ground tomato root-knot eelworm
(table two) as can be seen from the test results; 5 kinds of agricultural chemicals all have certain control efficiency to protection ground tomato root-knot eelworm disease; nematoda control pharmacy effect wherein of the present invention is best; disposable dispenser preventive effect reaches 95.42%; compared with the control; difference reaches utmost point significance level; next is the many granules of 10% happy lot; preventive effect to root knot nematode disease is 83.83%; difference reaches significance level compared with the control; the red granule of 5% legendary god of farming; 10% chlopyrifos granule; 2% abamectin emulsifiable concentrate is respectively the preventive effect of root knot nematode disease: 65.13%; 56.72%; 70.72% is comparatively remarkable compared with the control; but the red granule of 5% legendary god of farming; even belonging to the highly toxic pesticide preventive effect, 10% chlopyrifos granule reaches 100%; also be difficult on vegetables, using; before the regulation that national highly toxic pesticide is difficult for using on the vegetables is not put into effect; some vegetable grower uses this two kinds of agricultural chemicals throughout the year, and root-knot nematode produces resistance to these two kinds of agricultural chemicals, and control efficiency is unsatisfactory.
Though 2% abamectin emulsifiable concentrate is comparatively desirable to the root-knot nematode control efficiency, 2% Avermectin formulation is a missible oil, use, and inconvenience, 10% many granules of happy lot price is more expensive, has increased vegetable grower's input.Nematoda control medicament of the present invention; belong to biologic product; has the double effects that kills ovum and desinsection; we have carried out preliminary investigation to different disposal plant growing way and cell production; found that; use the sub-district of nematoda control medicament of the present invention; the plant growing way is strong; do not show the early ageing phenomenon, and other is handled in the appearance early ageing in various degree of plant strain growth later stage, cell production; use the sub-district of nematoda control medicament of the present invention; fruit does not have empty fruit, and output and commodity rate all are higher than other several processing, this shows nematoda control medicament of the present invention; control protection ground tomato root-knot eelworm disease is not only effective; vegetables are not polluted, and have volume increase, the effect of anti-plant early ageing.
In July, 2010, we did identical test getting at the gradually emerging vegetable grower Zhang Fuyou of northern Su Zhennan on the summer celery, and the result is almost consistent with result of the test on protection ground tomato.
Nematoda control chemical control celery root knot nematode disease field control effectiveness test of the present invention
Root knot nematode disease to vegetables particularly the celery of the tender succulence of root system cause harm, present the trend of cumulative year after year, celery grows and is obstructed after being caused harm by root-knot nematode, it is normal to be off color, during high humidity, plant is wilted, the root symptom, often due to illness former nematode is different and different.Celery generally needs 40 days from field planting to results, be difficult on celery, using hypertoxic high residue insecticide, research high effect nontoxic nematicide medicament, be to produce pressing for of pollution-free vegetable, my station in 2008 is at root-knot nematode cause harm characteristics and life habit, researched and developed the biopesticide that harmless noresidue prevents and treats biological root-knot nematode, in order to verify the effect of this agricultural chemicals, we the gradually emerging vegetable grower Zhang Fuyou autumn of Su Zhennan postponed booth celery field in north, Zoucheng City and tested autumn in 2008, autumn in 2009, we did identical test at Zoucheng City's peace town Sun Cun, by the effect of the clear and definite nematoda control medicament of the present invention of field trial.
1 materials and methods
1.1 reagent agent
The red granule (production of Shandong Province Hua Yang insecticide factory) of 5% legendary god of farming; 10% chlopyrifos granule (Zhejiang Xinan Chemical Industry Group Co.Ltd's production); (annotate: these two kinds of medicines belong to highly toxic pesticide, and national strictness is limited on the vegetables to be used, and wash down effect in order to verify nematode one, just as a comparison, test.); 2% abamectin emulsifiable concentrate (section's green bio Engineering Co., Ltd produces in the Jinan); The many granules of 10% happy lot (production of the former industry stone of Japanese strain formula commercial firm); Nematoda control medicament of the present invention (the innoxious nematoda control medicaments of research and development); The ck clear water in contrast.
1.2 for studying thing
Celery (the real celery in Jining)
1.3 situation experimental field
Experimental field be located at the gradually emerging village of Su Zhennan, north, Zoucheng City, physical features is smooth, and soil is cinnamon soil, and fertility is even, the content of organic matter 1.2%, and what the pH value was planted in 6.8,2006 years is cucumber, what planted in 2007 is tomato.Celery vernalization mid-August sowing, the early October field planting, seeding row spacing is 8cm * 10cm, fertilizer is used and is managed with conventional.
1.4 experimental scheme and arrangement
This test is provided with 6 processing, 3 repetitions, and district's group is arranged at random, zone leader 8.0m, wide 1.2m, area 9.6m
2Behind the whole good small sections of a vegetable plot, afternoon is according to sub-district design and the used dose of different disposal, spreads fertilizer over the fields (2% abamectin emulsifiable concentrate was mixed even back with 1: 10 with wheat bran and used) behind the medicament, and intertillage once irrigates small sections of a vegetable plot, wait water to soak down fully after, field planting again.Late January in next year begins results, the control efficiency between investigating during results.Test is handled and is seen table three for details:
Table three test is handled
1.5 investigation method
5 samplings of each sub-district diagonal during results, every 2 strain, totally 10 strains are carefully dug out plant with shovel, avoid hindering root as far as possible, after with clear water root being rinsed well, investigate grade scale (is unit with the root): 0 grade of root system does not produce fertile swollen lopsided warty spot; 1 grade: fertile swollen lopsided warty spot accounts for below 5% of whole root system; 3 grades: fertile swollen lopsided warty spot accounts for the 6%-10% of whole root system; 5 grades: fertile swollen lopsided warty spot accounts for the 11%-25% of whole root system; 7 grades: fertile swollen lopsided warty spot accounts for 26% of whole root system---and 50%.
2 interpretations of result
5 kinds of insecticides of table four are to the sick control efficiency of protection ground tomato root-knot eelworm
Result of the test from table four as can be seen, 5 kinds of agricultural chemicals all have certain control efficiency to the celery root knot nematode disease, nematoda control pharmacy effect wherein of the present invention is best, disposable dispenser preventive effect reaches 95.73%, compared with the control, difference reaches utmost point significance level, next is the many granules of 10% happy lot, preventive effect to root knot nematode disease is 84.96%, difference reaches significance level compared with the control, the red granule of 5% legendary god of farming, 10% chlopyrifos granule, 2% abamectin emulsifiable concentrate is respectively the preventive effect of root knot nematode disease: 68.25%, 58.94%, 67.72% is comparatively remarkable compared with the control, but the red granule of 5% legendary god of farming, even belonging to the highly toxic pesticide preventive effect, 10% chlopyrifos granule reaches 100%, also be difficult on vegetables, using, before the regulation that national highly toxic pesticide is difficult for using on the vegetables is not put into effect, some vegetable grower uses this two kinds of agricultural chemicals throughout the year, and root-knot nematode produces resistance to these two kinds of agricultural chemicals, and control efficiency is unsatisfactory.
Though 2% abamectin emulsifiable concentrate is comparatively desirable to the root-knot nematode control efficiency, 2% Avermectin formulation is a missible oil, uses inconvenience, and 10% many granules of happy lot price is more expensive, has increased vegetable grower's input.Nematoda control medicament of the present invention; belong to biologic product; has the double effects that kills ovum and desinsection; we have carried out preliminary investigation to different disposal plant growing way and cell production; found that; use the sub-district of nematoda control medicament of the present invention, the plant growing way is strong, and celery is light green; there is not yellow leaf; use the sub-district of nematoda control medicament of the present invention, output and commodity rate this shows nematoda control medicament of the present invention apparently higher than other several processing; control protection ground celery root knot nematode disease is not only effective; vegetables are not polluted, and have volume increase, improve the effect of celery quality.
In a word, the invention provides a kind of nematoda control medicament and production procedure thereof of avoiding contaminated environment, control efficiency to give prominence to.The above; only be a part of embodiment of the present invention, protection scope of the present invention is not limited thereto, and anyly is familiar with those skilled in the art in the technical scope that the present invention discloses; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.
Claims (4)
1. nematoda control medicament includes: actinomycetes, bacillus are the metabolite that main Pseudomonas and they are produced in vital movement, are equipped with the Chinese medicine wartwort and the biological insecticides Avermectin that the nematode adult there are killing action; Contain 2.0 hundred million actinomycetes, bacillus, Trichoderma as medium with every gram: 2% abamectin emulsifiable concentrate: wartwort is that 10: 1: 1 ratio proportioning is made.
2. a kind of nematoda control medicament according to claim 1, it is characterized in that: described Chinese medicine wartwort comprises wartwort saponin(e (phasin), Quercetin-3,5-digalactosyl glycosides (tithymalin), dihydrofucosterol (β-dihydrofucosterol), helioscopiol (hellscopiol), butyric acid and calcium malate.
3. a kind of nematoda control medicament according to claim 1, it is characterized in that: described bacillus can replace with bacillus subtilis, and wartwort can be with replacements such as sweet-scented oleanders, and 2% abamectin emulsifiable concentrate can replace with 1.8% abamectin emulsifiable concentrate.
4. the production procedure of a kind of nematoda control medicament according to claim 1 is characterized in that: (1) actinomycetes, bacillus are that main Pseudomonas carries out the viable bacteria cultivation, soaks activated viable bacteria in 3 hours after, it is standby that the interpolation peat composed of rotten mosses is made raw material 1; (2) after wartwort is gathered, pulverize, sieve, it is standby to make raw material 2; (3) 2% abamectin emulsifiable concentrates add organosilicon, and it is standby to make raw material 3; (4) with raw material 1: raw material 2: raw material 3 fully mixed at the shady place below 15 ℃ by 10: 1: 1, promptly got this product.
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| CN107788031A (en) * | 2017-10-31 | 2018-03-13 | 沿河土家族自治县双强牧业有限责任公司 | A kind of composition for treating flue-cured tobacco root knot nematode disease and preparation method thereof |
| CN110754484A (en) * | 2019-09-27 | 2020-02-07 | 贵州省果树科学研究所 | Mulberry scale control method for reducing pesticide residues in dragon orchard |
| CN110754484B (en) * | 2019-09-27 | 2021-05-25 | 贵州省果树科学研究所 | Mulberry scale control method for reducing pesticide residues in dragon orchard |
| CN110833075A (en) * | 2019-10-11 | 2020-02-25 | 北京泰克美高新技术有限公司 | Compound preparation and application thereof in treating pine plague wood infected with pine wilt disease |
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