CN101836577A - Cutting and breeding method for tree stocks of Populus tremula * P.tremuloides - Google Patents
Cutting and breeding method for tree stocks of Populus tremula * P.tremuloides Download PDFInfo
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- CN101836577A CN101836577A CN201010187327A CN201010187327A CN101836577A CN 101836577 A CN101836577 A CN 101836577A CN 201010187327 A CN201010187327 A CN 201010187327A CN 201010187327 A CN201010187327 A CN 201010187327A CN 101836577 A CN101836577 A CN 101836577A
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Abstract
The invention discloses a cutting and breeding method for tree stocks of Populus tremula * P. tremuloides, comprising the following steps: preparing a slotter, manufacturing cuttingwood, processing, cutting, managing after cutting, transplanting and planting in field, wherein in the step of manufacturing the cuttingwood, the current-year semi-lignified twigs of the Populus tremula * P. tremuloides are collected from March to May every year to serve as cutting, simple-ink single-leaf stalk sections are cut from the cuttings to serve as the cuttingwood. When the method of the invention is adopted for cutting and breeding the tree stocks of the Populus tremula * P. tremuloides, callus grows on the cuttings one week after cutting; roots begin to grow after 12 days; and after 18 days, the average length of the roots reaches 6.5cm, the average diameter of the roots reaches 0.18 cm, and the rooting rate reaches above 80%. The method of the invention improves the cutting survival rate of the Populus tremula * P. tremuloides to a greater degree; and in addition, the method of the invention also has the advantages of short emergency time, high rooting rate, simple and easily controlled technology, low breeding cost and the like.
Description
Technical field
The invention belongs to the propagation method of an elite stand nursery stock, relate in particular to the cuttage breeding method of American-European aspen hybrid nursery stock, belong to the clonal reproduction field of willow.
Background technology
(P.tremula * P.tremuloides) is the crossbreed of trembling poplar (P.tremuLa) and North America aspen (P.tremuLoides) to American-European aspen hybrid, be to utilize the time of decades and a large amount of funds that aspen cross-breeding and choiceness are selected by Finland forest-science institute, the aspen hybrid improved seeds that finishing screen is elected, have that growth is fast, cold-resistant, impoverishment tolerant, tree-like tall and big, characteristics such as adaptability is strong, be the fine tree species of northern planting out.In addition, this hybrid material is softer, fine and closely woven, pure white, and the trunk end is straight, is the quality raw materials of industry such as papermaking, plywood, particle board.Because American-European aspen hybrid timber look shallow, do not need the chlorinating agent bleaching during slurrying, compare with other pulpwood like this, not only price is low, and helps environmental protection, and simultaneously, the fiber characteristics of American-European aspen hybrid are suitable for some new paper technologies very much.Extensively introduced by many countries as good commerical tree species at present.
China is in 2000 and calendar year 2001, introduce 50 of American-European aspen hybrid choiceness by Forest Tree Genetics and Breeding section of Northeast Forestry University from Finland, by the method for group training and the cuttage afforestation of breeding in a large number and go up a hill, purpose is by the good willow hybrid of direct introducing, breed immediately and be used for production of forestry, can save the time and the funds that are used to study like this, shorten the breeding cycle of these seeds greatly; Also enriched simultaneously forest resources in China more, for forestry and forest product industry are brought ecology and economic benefit faster.Finland forest-science institute studies show that: the hybrid aspen is suitable for planting out, and Plain afforestation growing way is better, and its rotation and growth rate are all faster than general mountain region pin broad-leaved reproducting tree species.The climatic environment in a lot of areas, the China north is better than Finland, fertile soil, and the temperature height, sunshine is long, and vegetative period is also long, directly adopts and introduces good aspen hybridal clone afforestation, and its amount of growth significantly increases.Finland is located in high latitude area, the poor carbuncle of soil, and temperature condition is similar to the area, Northeast China.Therefore, these seeds be suitable in south, China Heilungkiang, Jilin, Liaoning and North China's plantation.
But American-European aspen hybrid belongs to the seeds that the cuttage difficulty is taken root, adopt the survival rate of cottage propagation low, this large tracts of land that has limited American-European aspen hybrid is greatly promoted, so provide the cuttage breeding method of a kind of survival rate height, American-European aspen hybrid nursery stock that cost is low to have important practical significance.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, and the cuttage breeding method of a kind of survival rate height, technology maturation, American-European aspen hybrid nursery stock that production cost is low is provided.
Technical problem to be solved by this invention is achieved through the following technical solutions:
The cuttage breeding method of a kind of American-European aspen hybrid nursery stock comprises: prepare slotting machine, the preparation plugged ear is handled plugged ear, and cuttage is inserted the back management, transplant planting; Wherein, described preparation plugged ear comprises: gather American-European aspen hybrid the annual 3-5 month and give birth to the semi-lignified spray then for transplanting a cutting, clip single-unit list base of leaf section is as plugged ear from transplant a cutting.
In order to reach better technique effect, preferred, clip length is that the single-unit list base of leaf section of 3～5cm is as plugged ear from transplant a cutting; What wherein, branch base portion degree of lignification was higher does not adopt with the too tender part in top; Preferred, leaf is kept full wafer leaf 1/3, all the other are cut; Wherein, flat declivity on the otch, upper cut is the 0.5cm place above bud, and lower cut is 2～3cm place below bud.
The present invention finds by a large amount of test, when adopting NAA 100ppm immersion treatment plugged ear in the time of 30 minutes, can improve the rooting rate of American-European aspen hybrid nursery stock significantly than other processing plugged ear, and root is sturdy, the transplanting survival rate height.
In addition, the inventor finds, gives birth to spray then as transplanting a cutting prepared rooting percent than higher the American-European aspen hybrid of clip in the 3～May in every year, is difficult to survive and adopt June and later single-unit spray to transplant a cutting cuttage.So cutting time of the present invention is preferably annual 3-5 month;
The oblique cutting method is adopted in described cuttage, and its cuttage degree of depth is preferably 1/3～2/5 of cutting length; Described cutting medium composition is preferably: the upper strata is river sand (thickness can be 5-10cm), and slotting tray bottom is covered the thick turfy soil of one deck 1-4cm (in order to water conservation).
Cuttage of the present invention can be finished in round-the-clock green house; Wherein, described slotting back management is preferably: preceding 3 days covering 50% sunshade nets after cuttage is finished shelter from heat or light, and temperature of shed is controlled between 25～35 ℃, and relative air humidity 70～90% is when temperature surpasses more than 35 ℃ room ventilation.Repeatedly spray water every day after the cuttage, keeps the blade face moistening.
The description of the preferred overall technical architecture of the present invention:
1. slotting machine is prepared
Slotting machine adopts the seedling-cultivating tray of 30 * 50 * 12cm specification, and slotting machine potassium permanganate solution with 0.1% before the matrix cuttage carries out spray disinfectant, and the cutting medium upper strata is a 8cm left and right sides river sand, and the 2cm left and right sides, lower floor shop turfy soil is in order to water conservation.Slotting machine is watered permeable with clear water before the cuttage.
2. cutting orchard
The fringe bar is provided by a miniature cutting orchard, as long as regularly prune, miniature cutting orchard just can provide endlessly gives birth to semi-lignified spray plugged ear then; Adopting the fringe maternal plant survive after the cuttage of spray plugged ear and rootage duration is approximately 8～15d, so behind first living then semi-lignified spray plugged ear cutting survival, can provide second batch of fringe bar to be used for cuttage.Build indoor cutting orchard like this, multiple batches of fringe bar just can be provided annual spring, adopt in addition a leaf one bud pattern the spray plugged ear (3～5cm), the pruning of science and water and fertilizer management, each maternal plant in the cutting orchard just might provide hundreds of or more fringe bar.
3. gather and transplant a cutting
3～May, the living then semi-lignified spray of the American-European aspen hybrid of clip is transplanted a cutting in green house, select the cloudy day to adopt bar, on maternal plant, choose the living then semi-lignified branch of robust growth, no damage by disease and insect, attention is suitably sprayed water to the cutting of shearing, In Shade preventing wilted, and keeps fresh state, gathers cuttage on the same day same day.Cutting is cut into the plugged ear of suitable specification, cutting length 3～5cm, diameter is about 0.2-0.3cm, flat declivity on the otch, upper cut is the 0.5cm place above bud, and lower cut is 2～3cm place below bud, crack-free.Every root cutting keeps single-unit, 1 blade, and every blade cuts off 2/3rds blades, and keeps petiole.
4. plugged ear is handled
The plugged ear of making is soaked earlier 10min in clear water, to reduce the influence of inhibitor to taking root, handle with plant hormone then.30 plugged ear bundles are a wisp, a bundle bundle cutting lower end is immersed in methyl (NAA) the 100ppm solution soaked 30 minutes, take out cuttage.
5. cuttage
The oblique cutting method is adopted in cuttage, and the cuttage degree of depth is 1/3～2/5 of a cutting length, stays a bud, each slotting machine cuttage 10 row, every row 10 strains, the strain spacing is 2.0～2.5cm, line space is 4.0～4.5cm, after the cuttage with hand with the river sand compacting.
6. insert the back management
Spray once permeable after inserting immediately.Cover with film on it for the blade face that makes cutting remains moisture state, shelter from heat or light with 50% shade net, surrounding air relative moisture 80%～90%, temperature are at 25～35 ℃, and repeatedly watered every day when the later stage, it was warm.
7. transplant planting
Transplant behind the rooting of cuttings, nutritive cube matrix is turfy soil and river sand, and ratio is 3: 1.Reject dead root, mashed root and the cutting of not taking root, seedling can be moved on to hardening outside the greenhouse in back 3 days, transplant after 7 days the seedling of not rudiment is sprayed the gibberellin of 200ppm to promote its sprouting in transplanting.
8. the afforestation of going up a hill
2 years living cuttage seeding, together with the nutritive cube plantation of going up a hill, afforestation.
Adopt the American-European aspen hybrid of the inventive method cutting propagation nursery stock, the cutting of 1 week just grows callus after carrying out cuttage, begin to take root after 12 days, the long 6.5cm of average root after 18 days, average root diameter reaches 0.18cm, rooting rate reaches more than 80%, improves American-European aspen hybrid cuttage survival rate largely.The inventive method seedling-growing time short (from cuttage to transplanting about 18 days), the rooting rate height, technology is simple, and reproductive-cost is low, is easy to grasp.
Embodiment
Embodiment 1
1, go up March then, the middle ten days, implement in Forestry University Forest Tree Genetic Breeding base northeastward.The American-European aspen hybrid of clip gives birth to spray then as cutting, the clip plugged ear, and every long 3-5cm keeps single-unit, single leaf.What branch base portion degree of lignification was higher does not adopt with the too tender part in top.
2, the cutting lower end is immersed in the NAA 100ppm solution and is soaked 30min, takes out.
3, the cutting medium upper strata is a 8cm left and right sides river sand, and the 3cm left and right sides, lower floor shop turfy soil is in order to water conservation.Slotting machine is watered permeable with clear water before the cuttage.
4, the oblique cutting method is adopted in cuttage, and the cuttage degree of depth is 1/3～2/5 of a plugged ear, stays a bud, each slotting machine cuttage 10 row, every row 10 strains, the strain spacing is 2.0～2.5cm, line space is 4.0～4.5cm, after the cuttage with hand with the river sand compacting.
5, temperature of shed 25-35 ℃, relative air humidity 70-90%, afternoon attention is ventilated, and the blade face water spray, keeps matrix and blade face moistening.Insert the back and 1 week grew callus, as seen take root after 12 days, the long 6.5cm of average root after 18 days, average root diameter reaches 0.18cm, and rooting rate can reach 94.5%.Be transplanted to 10 * 12cm size polypots (matrix is turfy soil: river sand=3: 1 adds a small amount of composite fertilizer), put alms bowl and warm canopy interior after 10 days transposition outdoor.
Embodiment 2
1, mid or late April then, implement in Forestry University Forest Tree Genetic Breeding base northeastward.The American-European aspen hybrid of clip gives birth to spray then as cutting, the clip plugged ear, and every long 3-5cm keeps single-unit, single leaf.What branch base portion degree of lignification was higher does not adopt with the too tender part in top.
2, the cutting lower end is immersed in the NAA 100ppm solution and is soaked 30min, takes out.
3, the cutting medium upper strata is a 8cm left and right sides river sand, and the 3cm left and right sides, lower floor shop turfy soil is in order to water conservation.Slotting machine is watered permeable with clear water before the cuttage.
4, the oblique cutting method is adopted in cuttage, and the cuttage degree of depth is 1/3～2/5 of a plugged ear, stays a bud, each slotting machine cuttage 10 row, every row 10 strains, the strain spacing is 2.0～2.5cm, line space is 4.0～4.5cm, after the cuttage with hand with the river sand compacting.
5, temperature of shed 25-35 ℃, relative air humidity 70-90%, afternoon attention is ventilated, and the blade face water spray, keeps matrix and blade face moistening.Insert the back and 1 week grew callus, as seen take root after 12 days, the long 6.2cm of average root after 18 days, average root diameter reaches 0.16cm, and rooting rate can reach 89.5%.Be transplanted to 10 * 12cm size polypots (matrix is turfy soil: river sand=3: 1 adds a small amount of composite fertilizer), put alms bowl and warm canopy interior after 10 days transposition outdoor.
Embodiment 3
1, mid or late May then, implement in Forestry University Forest Tree Genetic Breeding base northeastward.The American-European aspen hybrid of clip gives birth to spray then as cutting, the clip plugged ear, and every long 3-5cm keeps single-unit, single leaf.What branch base portion degree of lignification was higher does not adopt with the too tender part in top.
2, the cutting lower end is immersed in the NAA 100ppm solution and is soaked 30min, takes out.
3, the cutting medium upper strata is a 8cm left and right sides river sand, and the 3cm left and right sides, lower floor shop turfy soil is in order to water conservation.Slotting machine is watered permeable with clear water before the cuttage.
4, the oblique cutting method is adopted in cuttage, and the cuttage degree of depth is 1/3～2/5 of a plugged ear, stays a bud, each slotting machine cuttage 10 row, every row 10 strains, the strain spacing is 2.0～2.5cm, line space is 4.0～4.5cm, after the cuttage with hand with the river sand compacting.
5, temperature of shed 25-35 ℃, relative air humidity 70-90%, afternoon attention is ventilated, and the blade face water spray, keeps matrix and blade face moistening.Insert the back and 1 week grew callus, as seen take root after 14 days, the long 5.9cm of average root after 18 days, average root diameter reaches 0.12cm, and rooting rate reaches 85.9%.Be transplanted to 10 * 12cm size polypots (matrix is turfy soil: river sand=3: 1 adds a small amount of composite fertilizer), put alms bowl and warm canopy interior after 10 days transposition outdoor.
Comparative experimental example 1
Then mid or late June, implement in Forestry University Forest Tree Genetic Breeding base northeastward.The American-European aspen hybrid of clip gives birth to spray then as cutting, clip plugged ear, every long 6-15cm (not being single-unit list leaf); All the other conditions and embodiment 1-3 are identical.
Result of the test: insert back 10 and grow callus, as seen take root the long 4.1cm of average root after 21 days, average root diameter 0.08cm, rooting rate 52.6% after 17 days.
Comparative experimental example 2
Then July early and middle ten days, implement in Forestry University Forest Tree Genetic Breeding base northeastward.The American-European aspen hybrid of clip gives birth to spray then as cutting, clip plugged ear, every long 6-15cm (not being single-unit list leaf); All the other conditions and embodiment 1-3 are identical.
Result of the test: insert and grew callus in back 13 days, as seen take root the long 3.2cm of average root after 24 days, average root diameter 0.06cm, rooting rate 40.7% after 19 days.
Comparative experimental example 3
Then August early and middle ten days, implement in Forestry University Forest Tree Genetic Breeding base northeastward.The American-European aspen hybrid of clip gives birth to spray then as cutting, clip plugged ear, every long 6-15cm (not being single-unit list leaf); All the other conditions and embodiment 1-3 are identical.
Result of the test: insert and grew callus in back 16 days, as seen take root the long 2.1cm of average root after 26 days, average root diameter 0.05cm, rooting rate 12.6% after 23 days.
Comparative experimental example 4
Immerse in the 200ppm NAA solution and soak the 30min except will transplant a cutting the lower end in step 2, all the other are all identical with embodiment 1.
Result of the test: insert and grew callus in back 11 days, as seen take root the long 4.8cm of average root after 21 days, average root diameter 0.09cm, rooting rate 56.7% after 17 days.
Comparative experimental example 5
Immerse in the 300ppm NAA solution and soak the 30min except will transplant a cutting the lower end in step 2, all the other are all identical with embodiment 2.
Result of the test: insert and grew callus in back 13 days, as seen take root the long 4.1cm of average root after 22 days, average root diameter 0.78cm, rooting rate 43.7% after 18 days.
Claims (7)
- An American-European aspen hybrid (cuttage breeding method of nursery stock of P.tremula * P.tremuloides), comprising: prepare slotting machine, the preparation plugged ear is handled plugged ear, cuttage is inserted the back management, transplant planting; It is characterized in that described preparation plugged ear comprises: gather American-European aspen hybrid the annual 3-5 month and give birth to the semi-lignified spray then for transplanting a cutting, clip single-unit list base of leaf section is as plugged ear from transplant a cutting.
- 2. it is characterized in that in accordance with the method for claim 1: clip length is that the single-unit list base of leaf section of 3～5cm is as plugged ear from transplant a cutting.
- 3. in accordance with the method for claim 2, it is characterized in that: leaf is kept 1/3 of full wafer leaf, and all the other are cut; Wherein, flat declivity on the otch, upper cut is the 0.5cm place above bud, and lower cut is 2～3cm place below bud.
- 4. in accordance with the method for claim 1, it is characterized in that described processing plugged ear comprises: adopted NAA100ppm immersion treatment plugged ear 30 minutes.
- 5. in accordance with the method for claim 1, it is characterized in that: the oblique cutting method is adopted in described cuttage, and its cuttage degree of depth is 1/3～2/5 of a cutting length.
- 6. in accordance with the method for claim 1, it is characterized in that: described cutting medium consists of: the upper strata is a river sand, and thickness is 5-10cm, and slotting tray bottom is covered the thick turfy soil of one deck 1-4cm.
- 7. in accordance with the method for claim 1, it is characterized in that described slotting back management comprises: preceding 3 days covering 50% sunshade nets after cuttage is finished shelter from heat or light, and temperature of shed is controlled between 25～35 ℃, relative air humidity 70～90%; When temperature surpasses more than 35 ℃ room ventilation; Repeatedly spray water every day after the cuttage, keeps the blade face moistening.
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| CN2010101873276A CN101836577B (en) | 2010-05-27 | 2010-05-27 | Cutting and breeding method for tree stocks of Populus tremula P.tremuloides |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668979A (en) * | 2012-01-10 | 2012-09-19 | 河南科技大学 | Rooting culture method for poplar tissue culture seedlings |
| CN102860204A (en) * | 2012-09-18 | 2013-01-09 | 王继华 | Cuttage propagation method for styrax tonkinensis |
| WO2017089958A1 (en) * | 2015-11-23 | 2017-06-01 | Biotecnológica Empresarial Del Sur Spa | Method for propagation of poplar tree from leaf cuttings |
| CN107079761A (en) * | 2017-03-28 | 2017-08-22 | 枞阳县横山生态农业有限公司 | A kind of processing method of willow cutting |
-
2010
- 2010-05-27 CN CN2010101873276A patent/CN101836577B/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| 《东北林业大学》 20050630 刁桂平 欧美山杨杂种组培工厂化育苗技术研究 , * |
| 《内蒙古林业科技》 20010131 刘相民等 山杨嫩枝扦插新技术试验初报 第1页 1-7 , 第1期 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668979A (en) * | 2012-01-10 | 2012-09-19 | 河南科技大学 | Rooting culture method for poplar tissue culture seedlings |
| CN102668979B (en) * | 2012-01-10 | 2014-05-21 | 河南科技大学 | Rooting culture method for poplar tissue culture seedlings |
| CN102860204A (en) * | 2012-09-18 | 2013-01-09 | 王继华 | Cuttage propagation method for styrax tonkinensis |
| WO2017089958A1 (en) * | 2015-11-23 | 2017-06-01 | Biotecnológica Empresarial Del Sur Spa | Method for propagation of poplar tree from leaf cuttings |
| CN107079761A (en) * | 2017-03-28 | 2017-08-22 | 枞阳县横山生态农业有限公司 | A kind of processing method of willow cutting |
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| CN101836577B (en) | 2013-05-01 |
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