CN101835898B - 用于生物活性肽表达和纯化的可溶性标记 - Google Patents

用于生物活性肽表达和纯化的可溶性标记 Download PDF

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Publication number
CN101835898B
CN101835898B CN2008800253760A CN200880025376A CN101835898B CN 101835898 B CN101835898 B CN 101835898B CN 2008800253760 A CN2008800253760 A CN 2008800253760A CN 200880025376 A CN200880025376 A CN 200880025376A CN 101835898 B CN101835898 B CN 101835898B
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peptide
gln
seq
pro
gly
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CN101835898A (zh
Inventor
Q·程
L·J·德卡罗利斯
S·R·法内斯托克
T·M·格鲁伯
L·D·里斯
P·E·鲁维埃
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN2008800253760A 2007-07-25 2008-07-23 用于生物活性肽表达和纯化的可溶性标记 Expired - Fee Related CN101835898B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/782,836 US7678883B2 (en) 2007-07-25 2007-07-25 Solubility tags for the expression and purification of bioactive peptides
US11/782,836 2007-07-25
PCT/US2008/070802 WO2009015165A1 (en) 2007-07-25 2008-07-23 Solubility tags for the expression and purification of bioactive peptides

Publications (2)

Publication Number Publication Date
CN101835898A CN101835898A (zh) 2010-09-15
CN101835898B true CN101835898B (zh) 2013-01-02

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CN2008800253760A Expired - Fee Related CN101835898B (zh) 2007-07-25 2008-07-23 用于生物活性肽表达和纯化的可溶性标记

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US (2) US7678883B2 (enExample)
EP (1) EP2173877B1 (enExample)
JP (1) JP5453261B2 (enExample)
KR (1) KR20100057016A (enExample)
CN (1) CN101835898B (enExample)
AU (1) AU2008279186B2 (enExample)
CA (1) CA2689099A1 (enExample)
ES (1) ES2439702T3 (enExample)
IL (1) IL203002A0 (enExample)
WO (1) WO2009015165A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI567197B (zh) * 2014-12-29 2017-01-21 財團法人農業科技研究院 表現元件、表現卡匣、及含其之載體
CN109477113A (zh) * 2015-07-24 2019-03-15 爱沃克斯技术有限责任公司 催化活性蛋白质聚集体及其制备方法

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US9101687B2 (en) 2002-07-15 2015-08-11 University Of Leeds Beta sheet tapes ribbons in tissue engineering
US8586539B2 (en) 2002-07-15 2013-11-19 University Of Leeds Beta sheet tapes ribbons in tissue engineering
US7951559B2 (en) * 2007-07-25 2011-05-31 E.I. Du Pont De Nemours And Company Recombinant peptide production using a cross-linkable solubility tag
US7829311B2 (en) * 2007-07-25 2010-11-09 E.I. Du Pont De Nemours And Company Ketosteroid isomerase inclusion body tag engineered to be acid-resistant by replacing aspartates with glutamate
US7915011B2 (en) * 2009-03-05 2011-03-29 E.I. Du Pont De Nemours And Company Host cell modifications that improve peptide production and downstream processing
US9241987B2 (en) 2009-11-20 2016-01-26 The University Of Chicago Methods and compositions related to immunogenic fibrils
US8609621B2 (en) 2010-11-15 2013-12-17 E I Du Pont De Nemours And Company Acid-cleavable linkers exhibiting altered rates of acid hydrolysis
US9273336B2 (en) 2011-02-21 2016-03-01 E. I. Du Pont De Nemours And Company Recombinant host cells having an increase in buoyant density
US9115182B2 (en) 2011-06-21 2015-08-25 E I Du Pont De Nemours And Company Cysteine cross-linked structural peptides
US9062312B2 (en) 2011-06-21 2015-06-23 Danisco Us Inc. Fusion peptides comprising multi-functional peptidic solubility tags for efficient production, processing and surface applications
CN104755502B (zh) * 2012-10-12 2018-05-18 清华大学 多肽的产生和纯化方法
GB201306338D0 (en) 2013-04-08 2013-05-22 Univ Leeds Novel proteoglycans
CN104513298B (zh) * 2014-12-09 2018-07-17 山东信得科技股份有限公司 一种表达鸡传染性法氏囊可溶性vp 2-4-3多肽的方法
WO2020053239A1 (en) * 2018-09-11 2020-03-19 Nanotag Biotechnologies Gmbh Epitope tags recognized by specific binders
WO2023008415A1 (ja) * 2021-07-27 2023-02-02 ＳＴＡＮＤ Ｔｈｅｒａｐｅｕｔｉｃｓ株式会社 ペプチドタグおよび当該ペプチドタグをコードする核酸
US20250042946A1 (en) 2021-12-08 2025-02-06 Oakgrove Bio Llc Biological Production of Histidine-Rich Peptides
EP4567108A1 (en) * 2023-12-05 2025-06-11 Kutzner, Christoph Screening of target peptides using fusion polypeptides

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI567197B (zh) * 2014-12-29 2017-01-21 財團法人農業科技研究院 表現元件、表現卡匣、及含其之載體
CN109477113A (zh) * 2015-07-24 2019-03-15 爱沃克斯技术有限责任公司 催化活性蛋白质聚集体及其制备方法

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Publication number Publication date
AU2008279186B2 (en) 2013-09-05
EP2173877A1 (en) 2010-04-14
US7794979B2 (en) 2010-09-14
JP2010534477A (ja) 2010-11-11
WO2009015165A1 (en) 2009-01-29
IL203002A0 (en) 2011-08-01
AU2008279186A1 (en) 2009-01-29
JP5453261B2 (ja) 2014-03-26
EP2173877B1 (en) 2013-10-02
CA2689099A1 (en) 2009-01-29
CN101835898A (zh) 2010-09-15
US20090029412A1 (en) 2009-01-29
ES2439702T3 (es) 2014-01-24
KR20100057016A (ko) 2010-05-28
US20100136621A1 (en) 2010-06-03
US7678883B2 (en) 2010-03-16

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