JP5453261B2 - 生物活性ペプチドの発現および精製のための可溶性タグ - Google Patents

生物活性ペプチドの発現および精製のための可溶性タグ Download PDF

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Publication number
JP5453261B2
JP5453261B2 JP2010518341A JP2010518341A JP5453261B2 JP 5453261 B2 JP5453261 B2 JP 5453261B2 JP 2010518341 A JP2010518341 A JP 2010518341A JP 2010518341 A JP2010518341 A JP 2010518341A JP 5453261 B2 JP5453261 B2 JP 5453261B2
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peptide
interest
seq
inclusion body
fusion
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Expired - Fee Related
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Japanese (ja)
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JP2010534477A (ja
JP2010534477A5 (enExample
Inventor
ケイ・チェン
リンダ・ジェーン・デカロリス
ステファン・アール・ファーネストック
ターニャ・マリア・グリューバー
リーザ・ダイアン・ライス
ピエール・ウ・ルヴィール
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2010518341A 2007-07-25 2008-07-23 生物活性ペプチドの発現および精製のための可溶性タグ Expired - Fee Related JP5453261B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/782,836 US7678883B2 (en) 2007-07-25 2007-07-25 Solubility tags for the expression and purification of bioactive peptides
US11/782,836 2007-07-25
PCT/US2008/070802 WO2009015165A1 (en) 2007-07-25 2008-07-23 Solubility tags for the expression and purification of bioactive peptides

Publications (3)

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JP2010534477A JP2010534477A (ja) 2010-11-11
JP2010534477A5 JP2010534477A5 (enExample) 2011-08-18
JP5453261B2 true JP5453261B2 (ja) 2014-03-26

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JP2010518341A Expired - Fee Related JP5453261B2 (ja) 2007-07-25 2008-07-23 生物活性ペプチドの発現および精製のための可溶性タグ

Country Status (10)

Country Link
US (2) US7678883B2 (enExample)
EP (1) EP2173877B1 (enExample)
JP (1) JP5453261B2 (enExample)
KR (1) KR20100057016A (enExample)
CN (1) CN101835898B (enExample)
AU (1) AU2008279186B2 (enExample)
CA (1) CA2689099A1 (enExample)
ES (1) ES2439702T3 (enExample)
IL (1) IL203002A0 (enExample)
WO (1) WO2009015165A1 (enExample)

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US7915011B2 (en) * 2009-03-05 2011-03-29 E.I. Du Pont De Nemours And Company Host cell modifications that improve peptide production and downstream processing
WO2011063264A1 (en) 2009-11-20 2011-05-26 University Of Chicago Methods and compositions related to immunogenic fibrils
US8609621B2 (en) 2010-11-15 2013-12-17 E I Du Pont De Nemours And Company Acid-cleavable linkers exhibiting altered rates of acid hydrolysis
US9273336B2 (en) 2011-02-21 2016-03-01 E. I. Du Pont De Nemours And Company Recombinant host cells having an increase in buoyant density
US9062312B2 (en) * 2011-06-21 2015-06-23 Danisco Us Inc. Fusion peptides comprising multi-functional peptidic solubility tags for efficient production, processing and surface applications
US9115182B2 (en) * 2011-06-21 2015-08-25 E I Du Pont De Nemours And Company Cysteine cross-linked structural peptides
CN104755502B (zh) * 2012-10-12 2018-05-18 清华大学 多肽的产生和纯化方法
GB201306338D0 (en) 2013-04-08 2013-05-22 Univ Leeds Novel proteoglycans
CN104513298B (zh) * 2014-12-09 2018-07-17 山东信得科技股份有限公司 一种表达鸡传染性法氏囊可溶性vp 2-4-3多肽的方法
US10704051B2 (en) * 2014-12-29 2020-07-07 Agricultural Technology Research Institute Expression element, expression cassette, and vector containing the same
GB2540763A (en) * 2015-07-24 2017-02-01 Evocatal Gmbh Catalytically active protein aggregates and methods for producing the same
EP3849996A1 (en) * 2018-09-11 2021-07-21 NanoTag Biotechnologies GmbH Epitope tags recognized by specific binders
CA3227440A1 (en) * 2021-07-27 2023-02-02 Stand Therapeutics Co., Ltd. Peptide tag and nucleic acid encoding same
US20250042946A1 (en) 2021-12-08 2025-02-06 Oakgrove Bio Llc Biological Production of Histidine-Rich Peptides
EP4567108A1 (en) * 2023-12-05 2025-06-11 Kutzner, Christoph Screening of target peptides using fusion polypeptides

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Also Published As

Publication number Publication date
AU2008279186A1 (en) 2009-01-29
CA2689099A1 (en) 2009-01-29
EP2173877B1 (en) 2013-10-02
EP2173877A1 (en) 2010-04-14
JP2010534477A (ja) 2010-11-11
US20100136621A1 (en) 2010-06-03
AU2008279186B2 (en) 2013-09-05
US20090029412A1 (en) 2009-01-29
US7794979B2 (en) 2010-09-14
US7678883B2 (en) 2010-03-16
CN101835898B (zh) 2013-01-02
WO2009015165A1 (en) 2009-01-29
IL203002A0 (en) 2011-08-01
CN101835898A (zh) 2010-09-15
ES2439702T3 (es) 2014-01-24
KR20100057016A (ko) 2010-05-28

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