ATE457355T1 - Verfahren und dna-konstrukte zur produktion von polypeptiden mit hoher ausbeute - Google Patents
Verfahren und dna-konstrukte zur produktion von polypeptiden mit hoher ausbeuteInfo
- Publication number
- ATE457355T1 ATE457355T1 AT03734201T AT03734201T ATE457355T1 AT E457355 T1 ATE457355 T1 AT E457355T1 AT 03734201 T AT03734201 T AT 03734201T AT 03734201 T AT03734201 T AT 03734201T AT E457355 T1 ATE457355 T1 AT E457355T1
- Authority
- AT
- Austria
- Prior art keywords
- high yield
- producing high
- dna constructs
- yield polypeptides
- polypeptides
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 3
- 229920001184 polypeptide Polymers 0.000 title abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000004927 fusion Effects 0.000 abstract 1
- 210000003000 inclusion body Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/605—Glucagons
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/635—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Endocrinology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38337002P | 2002-05-24 | 2002-05-24 | |
| PCT/US2003/016643 WO2003100021A2 (en) | 2002-05-24 | 2003-05-23 | Methods and dna constructs for high yield production of polypeptides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE457355T1 true ATE457355T1 (de) | 2010-02-15 |
Family
ID=29584555
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03734201T ATE457355T1 (de) | 2002-05-24 | 2003-05-23 | Verfahren und dna-konstrukte zur produktion von polypeptiden mit hoher ausbeute |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US7501484B2 (de) |
| EP (1) | EP1532261B1 (de) |
| JP (2) | JP4636876B2 (de) |
| AT (1) | ATE457355T1 (de) |
| AU (1) | AU2003239895C1 (de) |
| CA (1) | CA2485835C (de) |
| DE (1) | DE60331226D1 (de) |
| DK (1) | DK1532261T3 (de) |
| ES (1) | ES2343061T3 (de) |
| WO (1) | WO2003100021A2 (de) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1411968B1 (de) * | 2001-07-31 | 2008-09-17 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | Glp 1 exendin 4 peptidanaloga und deren verwendungen |
| AU2003243316A1 (en) * | 2002-05-24 | 2003-12-12 | Nps Allelix Corp. | Method for enzymatic production of glp-2(1-33) and glp-2-(1-34) peptides |
| EP1554302A4 (de) * | 2002-05-24 | 2006-05-03 | Restoragen Inc | Verfahren und dna-konstrukte zur herstellung von polypeptiden mit hohen ausbeuten |
| ATE457355T1 (de) * | 2002-05-24 | 2010-02-15 | Medtronic Inc | Verfahren und dna-konstrukte zur produktion von polypeptiden mit hoher ausbeute |
| US20060128615A1 (en) * | 2002-09-18 | 2006-06-15 | Pierrette Gaudreau | Ghrh analogues |
| EP1529844B2 (de) † | 2003-07-21 | 2018-11-07 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Komplexbildung zur Stabilisierung und Reinigung eines Proteins von Interesse |
| ME01366B (me) * | 2003-11-21 | 2013-12-20 | Nps Allelix Corp | POSTUPAK ZA PROIZVODNJU PEPTIDA 2 NALIK NA GLUKAGON l NJIHOVIH ANALOGA |
| US20060069021A1 (en) * | 2004-08-13 | 2006-03-30 | Nastech Pharmaceutical Company Inc. | Compositions and methods for intranasal administration of inactive analogs of PTH or inactivated preparations of PTH or PTH analogs |
| GB0426160D0 (en) * | 2004-11-29 | 2004-12-29 | Era Plantech S L | Production of proteins |
| GB0426161D0 (en) * | 2004-11-29 | 2004-12-29 | Era Plantech S L | Protein isolation and purification |
| RU2478709C2 (ru) | 2005-07-21 | 2013-04-10 | Эбботт Лэборетриз | ЭКСПРЕССИЯ МНОЖЕСТВА ГЕНОВ, ВКЛЮЧАЯ sORF-КОНСТРУКЦИИ, И СПОСОБЫ ЭКСПРЕССИРОВАНИЯ ИММУНОГЛОБУЛИНА |
| US7736633B2 (en) | 2005-09-28 | 2010-06-15 | E.I. Du Pont De Nemours And Company | Method for enhancing effects of colorants and conditioners |
| US7662913B2 (en) | 2006-10-19 | 2010-02-16 | E. I. Du Pont De Nemours And Company | Cystatin-based peptide tags for the expression and purification of bioactive peptides |
| US7732569B2 (en) | 2006-10-19 | 2010-06-08 | E.I. Du Pont De Nemours And Company | Zein-based peptide tags for the expression and purification of bioactive peptides |
| US20100234568A1 (en) * | 2006-10-19 | 2010-09-16 | Linda Jane Decarolis | Identification of peptide tags for the production of insoluble peptides by sequence scanning |
| US7678883B2 (en) * | 2007-07-25 | 2010-03-16 | E.I. Du Pont De Nemours And Company | Solubility tags for the expression and purification of bioactive peptides |
| US7951559B2 (en) * | 2007-07-25 | 2011-05-31 | E.I. Du Pont De Nemours And Company | Recombinant peptide production using a cross-linkable solubility tag |
| US7829311B2 (en) | 2007-07-25 | 2010-11-09 | E.I. Du Pont De Nemours And Company | Ketosteroid isomerase inclusion body tag engineered to be acid-resistant by replacing aspartates with glutamate |
| EP2363469B1 (de) | 2008-10-21 | 2018-08-15 | The Chemo-Sero-Therapeutic Research Institute | Verfahren zur herstellung eines einschlusskörperbildenden proteins |
| US9062312B2 (en) | 2011-06-21 | 2015-06-23 | Danisco Us Inc. | Fusion peptides comprising multi-functional peptidic solubility tags for efficient production, processing and surface applications |
| KR20140148420A (ko) * | 2012-03-19 | 2014-12-31 | 마들렌 파마수티컬스 피티와이 리미티드 | 재조합 펩티드의 생산 방법 |
| EP2792686A1 (de) | 2013-04-17 | 2014-10-22 | Heinrich-Heine-Universität Düsseldorf | Verfahren zur Expression von Peptiden und Proteinen |
| JP6548063B2 (ja) * | 2014-09-11 | 2019-07-24 | 国立大学法人 岡山大学 | タンパク質、融合タンパク質の製造法及び精製法 |
| RU2562869C1 (ru) * | 2014-10-31 | 2015-09-10 | Федеральное государственное бюджетное учреждение науки Институт биохимии им. А.Н. Баха Российской академии наук (ИНБИ РАН) | ИНТЕГРАТИВНАЯ ГЕНЕТИЧЕСКАЯ КОНСТРУКЦИЯ pQ-SRUS ДЛЯ ПОЛУЧЕНИЯ ШТАММОВ ДРОЖЖЕЙ Yarrowia lipolytica, ОБЛАДАЮЩИХ СПОСОБНОСТЬЮ К ГОМОЛОГИЧНОЙ РЕКОМБИНАЦИИ ГЕНОМА МИТОХОНДРИЙ ЗА СЧЁТ ЭКСПРЕССИИ ГЕНА БЕЛКА RecA БАКТЕРИАЛЬНОГО ПРОИСХОЖДЕНИЯ |
| SG10202111844VA (en) | 2015-01-09 | 2021-12-30 | Adalta Ltd | Cxcr4 binding molecules |
| CN108640972B (zh) * | 2018-05-17 | 2021-07-30 | 中国药科大学 | 与vista具有亲和力的短肽及其用途 |
| CN110656119B (zh) * | 2019-10-09 | 2021-05-25 | 武汉博欧特生物科技有限公司 | 一种用于观察细菌蛋白定位的表达载体及其构建方法 |
| MX2023011588A (es) | 2021-03-31 | 2023-10-10 | Biological E Ltd | Constructos y metodos para aumentar la expresion de polipeptidos. |
| EP4444739A2 (de) | 2021-12-08 | 2024-10-16 | Oakgrove Bio LLC | Biologische herstellung von histidinreichen peptiden |
| WO2024010489A2 (en) * | 2022-07-08 | 2024-01-11 | Shemyakin-Ovchinnikov Institute Of Bioorganic Chemistry Of The Russian Academy Of Sciences | New hybrid protein cbd-hs-es-glp1, recombinant plasmid for its production, escherichia coli producer strain and method of producing glp-1 polypeptide |
| WO2024261777A1 (en) * | 2023-06-19 | 2024-12-26 | Biological E Limited | Expression cassettes for protein expression, method(s) and application(s) thereof |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9203803D0 (en) * | 1992-02-21 | 1992-04-08 | Wellcome Found | A recombinant polypeptide |
| US5814603A (en) * | 1992-06-12 | 1998-09-29 | Affymax Technologies N.V. | Compounds with PTH activity |
| US5352771A (en) * | 1992-08-31 | 1994-10-04 | Iowa State University Research Foundation Inc. | Hydrolysis of peptide bonds using Pt (II) and Pd (II) complexes |
| ATE373708T1 (de) * | 1993-08-16 | 2007-10-15 | Res Dev Foundation | Ein homeobox-faktor der insulin-expression in pankreatischen inselnzellen stimuliert |
| US5747453A (en) * | 1995-06-06 | 1998-05-05 | Alza Corporation | Method for increasing the electrotransport flux of polypeptides |
| WO1998030684A1 (en) * | 1997-01-08 | 1998-07-16 | Life Technologies, Inc. | Methods for production of proteins |
| WO1998052966A1 (en) * | 1997-05-19 | 1998-11-26 | The Johns Hopkins University School Of Medecine | Tissue specific prodrug |
| WO2002046227A2 (en) * | 2000-12-07 | 2002-06-13 | Eli Lilly And Company | Glp-1 fusion proteins |
| ATE457355T1 (de) * | 2002-05-24 | 2010-02-15 | Medtronic Inc | Verfahren und dna-konstrukte zur produktion von polypeptiden mit hoher ausbeute |
-
2003
- 2003-05-23 AT AT03734201T patent/ATE457355T1/de not_active IP Right Cessation
- 2003-05-23 DK DK03734201.1T patent/DK1532261T3/da active
- 2003-05-23 CA CA2485835A patent/CA2485835C/en not_active Expired - Lifetime
- 2003-05-23 WO PCT/US2003/016643 patent/WO2003100021A2/en not_active Ceased
- 2003-05-23 EP EP03734201A patent/EP1532261B1/de not_active Expired - Lifetime
- 2003-05-23 ES ES03734201T patent/ES2343061T3/es not_active Expired - Lifetime
- 2003-05-23 AU AU2003239895A patent/AU2003239895C1/en not_active Expired
- 2003-05-23 JP JP2004508263A patent/JP4636876B2/ja not_active Expired - Fee Related
- 2003-05-23 DE DE60331226T patent/DE60331226D1/de not_active Expired - Lifetime
-
2004
- 2004-11-24 US US10/997,078 patent/US7501484B2/en not_active Expired - Lifetime
-
2009
- 2009-01-23 US US12/358,966 patent/US9322025B2/en not_active Expired - Lifetime
-
2010
- 2010-08-13 JP JP2010181250A patent/JP5455840B2/ja not_active Expired - Fee Related
-
2016
- 2016-04-25 US US15/137,134 patent/US9951368B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US20090170192A1 (en) | 2009-07-02 |
| WO2003100021A2 (en) | 2003-12-04 |
| AU2003239895C1 (en) | 2010-01-07 |
| EP1532261A4 (de) | 2006-08-23 |
| EP1532261B1 (de) | 2010-02-10 |
| WO2003100021A3 (en) | 2004-09-10 |
| JP2006508639A (ja) | 2006-03-16 |
| ES2343061T3 (es) | 2010-07-22 |
| EP1532261A2 (de) | 2005-05-25 |
| DE60331226D1 (de) | 2010-03-25 |
| US7501484B2 (en) | 2009-03-10 |
| US20170029860A1 (en) | 2017-02-02 |
| US9951368B2 (en) | 2018-04-24 |
| AU2003239895B2 (en) | 2009-06-04 |
| CA2485835A1 (en) | 2003-12-04 |
| JP4636876B2 (ja) | 2011-02-23 |
| CA2485835C (en) | 2018-03-06 |
| US9322025B2 (en) | 2016-04-26 |
| DK1532261T3 (da) | 2010-05-31 |
| JP5455840B2 (ja) | 2014-03-26 |
| JP2011004757A (ja) | 2011-01-13 |
| AU2003239895A1 (en) | 2003-12-12 |
| US20050221444A1 (en) | 2005-10-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |