DE602004029936D1 - Schnelles screening-verfahren translationaler fusionspartner zur produktion rekombinanter proteine sowie durch screening damit erhaltene translationale fusionspartner - Google Patents

Schnelles screening-verfahren translationaler fusionspartner zur produktion rekombinanter proteine sowie durch screening damit erhaltene translationale fusionspartner

Info

Publication number
DE602004029936D1
DE602004029936D1 DE200460029936 DE602004029936T DE602004029936D1 DE 602004029936 D1 DE602004029936 D1 DE 602004029936D1 DE 200460029936 DE200460029936 DE 200460029936 DE 602004029936 T DE602004029936 T DE 602004029936T DE 602004029936 D1 DE602004029936 D1 DE 602004029936D1
Authority
DE
Germany
Prior art keywords
translational fusion
screening
production
recombinant proteins
fusion partners
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE200460029936
Other languages
English (en)
Inventor
Jung-Hoon Sohn
Eui-Sung Choi
Jung-Hoon Bae
Eung-Suck Lee
Mi-Kyung Shin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Research Institute of Bioscience and Biotechnology KRIBB
Original Assignee
Korea Research Institute of Bioscience and Biotechnology KRIBB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020040003610A external-priority patent/KR100626753B1/ko
Priority claimed from KR1020040003957A external-priority patent/KR20050076139A/ko
Application filed by Korea Research Institute of Bioscience and Biotechnology KRIBB filed Critical Korea Research Institute of Bioscience and Biotechnology KRIBB
Publication of DE602004029936D1 publication Critical patent/DE602004029936D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE200460029936 2004-01-17 2004-12-30 Schnelles screening-verfahren translationaler fusionspartner zur produktion rekombinanter proteine sowie durch screening damit erhaltene translationale fusionspartner Active DE602004029936D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020040003610A KR100626753B1 (ko) 2004-01-17 2004-01-17 재조합 단백질 생산용 맞춤형 단백질융합인자의 초고속선별 방법
KR1020040003957A KR20050076139A (ko) 2004-01-19 2004-01-19 단백질 분비 생산을 유도하는 단백질융합인자
PCT/KR2004/003517 WO2005068658A1 (en) 2004-01-17 2004-12-30 Rapid screening method of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom

Publications (1)

Publication Number Publication Date
DE602004029936D1 true DE602004029936D1 (de) 2010-12-16

Family

ID=34797866

Family Applications (1)

Application Number Title Priority Date Filing Date
DE200460029936 Active DE602004029936D1 (de) 2004-01-17 2004-12-30 Schnelles screening-verfahren translationaler fusionspartner zur produktion rekombinanter proteine sowie durch screening damit erhaltene translationale fusionspartner

Country Status (7)

Country Link
US (2) US8673822B2 (de)
EP (2) EP2275556B1 (de)
JP (2) JP5148879B2 (de)
AT (1) ATE486943T1 (de)
DE (1) DE602004029936D1 (de)
DK (2) DK1713930T3 (de)
WO (1) WO2005068658A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2275556B1 (de) 2004-01-17 2013-04-24 Korea Research Institute of Bioscience and Biotechnology Schnell-Screeningverfahren für Translationsfusionspartner zur Herstellung rekombinanter Proteine und daraus gescreente Translationsfusionspartner
KR20070009269A (ko) * 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
CN102239183B (zh) * 2008-12-04 2017-06-30 韩国生命工学研究院 大量分泌的蛋白的筛选和它们作为融合配偶体在重组蛋白制备中应用
WO2012060666A2 (ko) 2010-11-04 2012-05-10 한국생명공학연구원 효모에서 인체 상피세포 성장인자를 대량 생산하는 방법
CN104694523A (zh) * 2015-03-21 2015-06-10 江南大学 一种精氨酸脱亚胺酶的胞外表达方法及其应用
WO2023118670A1 (en) * 2021-12-23 2023-06-29 Helsingin Yliopisto Method for screening a signal peptide for efficient expression and secretion of a heterologous polypeptide in mammalian cells

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60501140A (ja) 1983-04-22 1985-07-25 アムジエン 酵母による外因性ポリペプチドの分泌
ATE110111T1 (de) * 1986-05-02 1994-09-15 Gist Brocades Nv Sekretionssignal-selektionsvektoren für extrazelluläre proteinsynthese in bazillen.
FR2648713B1 (fr) * 1989-06-26 1994-06-10 Commissariat Energie Atomique Proteines hybrides entre une enzyme extracytoplasmique et une autre proteine, leur procede de preparation, ainsi que leur application comme agent de diagnostic
US5212058A (en) * 1990-05-09 1993-05-18 Massachusetts Institute Of Technology Nucleic acid encoding ubiquitin-specific proteases
US5547871A (en) * 1993-01-25 1996-08-20 American Cyanamid Company Heterologous signal sequences for secretion of insect controlling proteins
US5536637A (en) * 1993-04-07 1996-07-16 Genetics Institute, Inc. Method of screening for cDNA encoding novel secreted mammalian proteins in yeast
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
EP0662515B1 (de) * 1993-12-10 2000-11-15 Korea Institute Of Science And Technology Signalsequenzen für die Secretion heterologer Proteine von Hefe
JP3730256B2 (ja) 1995-02-03 2005-12-21 旭硝子株式会社 分泌シグナル遺伝子およびそれを有する発現ベクター
EP1170366A1 (de) 1996-04-24 2002-01-09 Genetics Institute, Inc. Secreted proteins obtained by using Yeast invertase gene as reporter system
WO1997040146A1 (en) 1996-04-24 1997-10-30 Genetics Institute, Inc. Yeast invertase gene as reporter system for isolating cytokines
US5952171A (en) * 1996-11-19 1999-09-14 Millennium Biotherapeutics, Inc. Method for identifying genes encoding secreted or membrane-associated proteins
US6136569A (en) * 1997-02-27 2000-10-24 Genentech, Inc. Method of creating a cDNA library enriched in signal sequences
US6548633B1 (en) * 1998-12-22 2003-04-15 Genset, S.A. Complementary DNA's encoding proteins with signal peptides
US20060009633A9 (en) * 1997-11-13 2006-01-12 Genset, S.A. Complementary DNAs encoding proteins with signal peptides
US6150098A (en) * 1998-02-20 2000-11-21 Amgen Inc. Methods for identifying novel secreted mammalian polypeptides
WO1999049028A1 (en) 1998-03-23 1999-09-30 Genentech, Inc. Method of selection for genes encoding secreted and transmembrane proteins
WO2001000803A2 (en) * 1999-06-25 2001-01-04 Genset Apolipoprotein a-iv-related protein: polypeptide, polynucleotide sequences and biallelic markers thereof
US6753314B1 (en) * 1999-04-01 2004-06-22 Curagen Corporation Protein-protein complexes and methods of using same
US7029842B2 (en) * 2000-04-07 2006-04-18 Novozymes A/S Signal sequence trapping
PT1276857E (pt) * 2000-04-07 2008-11-13 Novozymes As Captura da sequência do sinal
US20020127557A1 (en) * 2001-03-09 2002-09-12 Ruoying Tan Method for identification of cDNAs encoding signal peptides
US7314974B2 (en) * 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
EP2275556B1 (de) 2004-01-17 2013-04-24 Korea Research Institute of Bioscience and Biotechnology Schnell-Screeningverfahren für Translationsfusionspartner zur Herstellung rekombinanter Proteine und daraus gescreente Translationsfusionspartner
KR20070009269A (ko) * 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
CN102239183B (zh) * 2008-12-04 2017-06-30 韩国生命工学研究院 大量分泌的蛋白的筛选和它们作为融合配偶体在重组蛋白制备中应用

Also Published As

Publication number Publication date
DK1713930T3 (da) 2011-01-17
US9012367B2 (en) 2015-04-21
US8673822B2 (en) 2014-03-18
US20070275385A1 (en) 2007-11-29
US20140220631A1 (en) 2014-08-07
JP5662363B2 (ja) 2015-01-28
JP2007517519A (ja) 2007-07-05
EP1713930A1 (de) 2006-10-25
EP2275556A1 (de) 2011-01-19
EP1713930A4 (de) 2007-02-28
JP2012105675A (ja) 2012-06-07
WO2005068658A1 (en) 2005-07-28
DK2275556T3 (da) 2013-07-08
JP5148879B2 (ja) 2013-02-20
EP1713930B1 (de) 2010-11-03
ATE486943T1 (de) 2010-11-15
EP2275556B1 (de) 2013-04-24

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