ATE374825T1 - Verwendung von caspasen zur herstellung von reifen rekombinanten fusionsproteinen - Google Patents
Verwendung von caspasen zur herstellung von reifen rekombinanten fusionsproteinenInfo
- Publication number
- ATE374825T1 ATE374825T1 AT04713540T AT04713540T ATE374825T1 AT E374825 T1 ATE374825 T1 AT E374825T1 AT 04713540 T AT04713540 T AT 04713540T AT 04713540 T AT04713540 T AT 04713540T AT E374825 T1 ATE374825 T1 AT E374825T1
- Authority
- AT
- Austria
- Prior art keywords
- fusion proteins
- caspases
- recombinant fusion
- produce mature
- mature recombinant
- Prior art date
Links
- 102000011727 Caspases Human genes 0.000 title abstract 3
- 108010076667 Caspases Proteins 0.000 title abstract 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 230000004927 fusion Effects 0.000 abstract 1
- 102000037865 fusion proteins Human genes 0.000 abstract 1
- 108020001507 fusion proteins Proteins 0.000 abstract 1
- 230000035800 maturation Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6472—Cysteine endopeptidases (3.4.22)
- C12N9/6475—Interleukin 1-beta convertase-like enzymes (3.4.22.10; 3.4.22.36; 3.4.22.63)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5415—Leukaemia inhibitory factor [LIF]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP03447034 | 2003-02-21 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE374825T1 true ATE374825T1 (de) | 2007-10-15 |
Family
ID=32893040
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04713540T ATE374825T1 (de) | 2003-02-21 | 2004-02-23 | Verwendung von caspasen zur herstellung von reifen rekombinanten fusionsproteinen |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20060099689A1 (de) |
| EP (1) | EP1597369B1 (de) |
| JP (1) | JP2006518207A (de) |
| AT (1) | ATE374825T1 (de) |
| AU (1) | AU2004213586B2 (de) |
| CA (1) | CA2511855A1 (de) |
| DE (1) | DE602004009292T2 (de) |
| DK (1) | DK1597369T3 (de) |
| NO (1) | NO20053033D0 (de) |
| NZ (1) | NZ541551A (de) |
| PT (1) | PT1597369E (de) |
| WO (1) | WO2004074488A1 (de) |
| ZA (1) | ZA200504958B (de) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2004232405B2 (en) | 2003-04-23 | 2008-06-19 | F. Hoffman - La Roche Ltd. | Cleavage of fusion proteins using granzyme B protease |
| WO2005049847A1 (en) * | 2003-11-24 | 2005-06-02 | Novo Nordisk A/S | Fusion proteins and methods of cleavage of such proteins |
| JP5990102B2 (ja) | 2009-09-29 | 2016-09-07 | ユニフェルシテイト ヘント | ホスホ−６−マンノースへのマンノース−１−ホスホ−６−マンノース結合の加水分解 |
| EP2512505A2 (de) * | 2009-12-16 | 2012-10-24 | Allergan, Inc. | Modifizierte clostridialtoxine mit integrierter domäne zur bindung an der proteasenspaltungsstelle |
| KR101189193B1 (ko) * | 2010-06-23 | 2012-10-09 | 한국과학기술연구원 | 저분자열충격단백질이 포함된 융합 단백질, 이에 의해 구성된 케이지 단백질 및 이의 신규한 용도 |
| WO2019126960A1 (zh) * | 2017-12-25 | 2019-07-04 | 清华大学 | 一种具有蛋白酶活性的多肽及其应用 |
| KR20200080749A (ko) * | 2018-12-27 | 2020-07-07 | 주식회사 폴루스 | N-말단의 메티오닌이 절단된 목적 단백질 발현용 유전자 발현 카세트 및 이를 이용하여 n-말단의 메티오닌이 절단된 목적 단백질을 생산하는 방법 |
| US20220380739A1 (en) | 2019-08-14 | 2022-12-01 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Caspase-2 variants |
| WO2021193566A1 (ja) * | 2020-03-23 | 2021-09-30 | 国立大学法人京都工芸繊維大学 | 新規抗体及びその用途 |
| EP4000596A1 (de) * | 2020-11-17 | 2022-05-25 | The Boots Company plc | Tetrapeptid und zusammensetzungen mit tetrapeptiden |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2900806C3 (de) * | 1979-01-11 | 1982-03-18 | B. Braun Melsungen Ag, 3508 Melsungen | Vorrichtung zum Sammeln einer Körperflüssigkeit, insbesondere Urin |
| EP0207044A1 (de) * | 1985-06-20 | 1986-12-30 | Monsanto Company | Freisetzung von Peptiden aus Polypeptiden |
| US5087564A (en) * | 1985-06-20 | 1992-02-11 | Monsanto Company | Release of recombinant peptides from polypeptides using V8 endopeptidase |
| US4743679A (en) * | 1986-02-24 | 1988-05-10 | Creative Biomolecules, Inc. | Process for producing human epidermal growth factor and analogs thereof |
| CA1340173C (en) * | 1987-03-10 | 1998-12-08 | Chudi Guan | Production and purification of a protein fused to a binding protein |
| US5643758A (en) * | 1987-03-10 | 1997-07-01 | New England Biolabs, Inc. | Production and purification of a protein fused to a binding protein |
| AU4838690A (en) * | 1988-12-09 | 1990-06-26 | Smithkline Beecham Corporation | Use of hiv protease cleavage site to express mature proteins |
| US6077694A (en) * | 1990-09-21 | 2000-06-20 | The United States Of America As Represented By The Department Of Health And Human Services | Method for over-expression and rapid purification of biosynthetic proteins |
| JP2001526533A (ja) * | 1997-04-23 | 2001-12-18 | フラームス・インテルウニフェルシタイル・インスティチュート・フォール・ビオテヒノロヒー | λ様プロモーターを有する遺伝子の誘導性発現のための調節系 |
| US6083715A (en) * | 1997-06-09 | 2000-07-04 | Board Of Regents, The University Of Texas System | Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells |
| CA2295957C (en) * | 1997-08-07 | 2009-09-22 | Toray Industries, Inc. | Canine interleukin 18, canine interleukin 1.beta. converting enzyme, dna sequences encoding these, interleukin 18 production method and canine immune disease remedy |
| US5989868A (en) * | 1997-09-12 | 1999-11-23 | The Board Of Regents Of The University Of Oklahoma | Fusion protein systems designed to increase soluble cytoplasmic expression of heterologous proteins in esherichia coli |
| US6379950B1 (en) * | 1998-01-09 | 2002-04-30 | Thomas Jefferson University | Recombinant, active caspases and uses thereof |
| US6916917B1 (en) * | 1998-11-17 | 2005-07-12 | California Institute Of Technology | Chimeric pro-caspases and methods of using same |
| IL129427A0 (en) * | 1999-04-13 | 2000-02-17 | Yeda Res & Dev | Preparation of biologically active molecules |
| US6420157B1 (en) * | 1999-04-30 | 2002-07-16 | Ortho-Mcneil Pharmaceutical, Inc. | Zymogen activation system |
| WO2000073437A1 (en) * | 1999-05-27 | 2000-12-07 | Merck Frosst Canada & Co. | Assays for caspase activity using green fluorescent proteins |
| US7262005B1 (en) * | 2000-02-04 | 2007-08-28 | Aurora Biosciences Corporation | Methods of protein destabilization and uses thereof |
| US6890745B1 (en) * | 2000-07-19 | 2005-05-10 | Chemicon International, Inc. | Protease specific cleavable luciferases and methods of use thereof |
| US20020073441A1 (en) * | 2000-12-13 | 2002-06-13 | Ross Brian D. | Compositions and methods for detecting proteolytic activity |
| US7354735B2 (en) * | 2001-05-24 | 2008-04-08 | Hong Kong University Of Science And Technology | Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis |
| JP2004537313A (ja) * | 2001-07-31 | 2004-12-16 | クローンテック ラボラトリーズ インク． | 細胞内のプロテアーゼ活性を検出する方法 |
| WO2003033650A2 (en) * | 2001-10-15 | 2003-04-24 | Stratagene | Recombinant renilla reniformis system for bioluminescence resonance energy transfer |
-
2004
- 2004-02-23 DK DK04713540T patent/DK1597369T3/da active
- 2004-02-23 DE DE602004009292T patent/DE602004009292T2/de not_active Expired - Lifetime
- 2004-02-23 NZ NZ541551A patent/NZ541551A/en unknown
- 2004-02-23 AT AT04713540T patent/ATE374825T1/de not_active IP Right Cessation
- 2004-02-23 US US10/543,587 patent/US20060099689A1/en not_active Abandoned
- 2004-02-23 WO PCT/EP2004/001759 patent/WO2004074488A1/en active IP Right Grant
- 2004-02-23 AU AU2004213586A patent/AU2004213586B2/en not_active Ceased
- 2004-02-23 CA CA002511855A patent/CA2511855A1/en not_active Abandoned
- 2004-02-23 PT PT04713540T patent/PT1597369E/pt unknown
- 2004-02-23 EP EP04713540A patent/EP1597369B1/de not_active Expired - Lifetime
- 2004-02-23 JP JP2006501924A patent/JP2006518207A/ja not_active Withdrawn
-
2005
- 2005-06-17 ZA ZA200504958A patent/ZA200504958B/en unknown
- 2005-06-20 NO NO20053033A patent/NO20053033D0/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004074488A1 (en) | 2004-09-02 |
| NO20053033L (no) | 2005-06-20 |
| ZA200504958B (en) | 2006-04-26 |
| US20060099689A1 (en) | 2006-05-11 |
| CA2511855A1 (en) | 2004-09-02 |
| NZ541551A (en) | 2007-02-23 |
| DE602004009292T2 (de) | 2009-03-19 |
| EP1597369A1 (de) | 2005-11-23 |
| AU2004213586B2 (en) | 2009-01-15 |
| PT1597369E (pt) | 2008-01-10 |
| EP1597369B1 (de) | 2007-10-03 |
| NO20053033D0 (no) | 2005-06-20 |
| AU2004213586A1 (en) | 2004-09-02 |
| DK1597369T3 (da) | 2008-02-04 |
| JP2006518207A (ja) | 2006-08-10 |
| DE602004009292D1 (de) | 2007-11-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1597369 Country of ref document: EP |
|
| REN | Ceased due to non-payment of the annual fee |