CN101701243A - 生物催化法生产r-扁桃酸及其衍生物的方法 - Google Patents
生物催化法生产r-扁桃酸及其衍生物的方法 Download PDFInfo
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- CN101701243A CN101701243A CN200910154484A CN200910154484A CN101701243A CN 101701243 A CN101701243 A CN 101701243A CN 200910154484 A CN200910154484 A CN 200910154484A CN 200910154484 A CN200910154484 A CN 200910154484A CN 101701243 A CN101701243 A CN 101701243A
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- Prior art keywords
- amygdalic acid
- reaction
- enzyme
- acid
- mandelonitrile
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- Enzymes And Modification Thereof (AREA)
Abstract
Description
characteristics | Results |
Gram staining | - |
DP3-300 | - |
Glucoese(oxidative) | - |
Growth Control | + |
Acetamide | + |
Esculin | - |
Plant Indican | - |
Urea | - |
Citrate | + |
Malonate | + |
Phenylalanine | - |
Polymyxin B | - |
Lactose | - |
Maltose | - |
Mannitol | - |
Xylose | - |
Raffinose | - |
characteristics | Results |
Sorbitol | - |
Sucose | - |
Inositol | - |
Adonitol | - |
p-Coumaric | - |
H2S | - |
o-Nitrophenol | - |
Rhamnose | - |
Arabinose | - |
Glucose(Fermentative) | - |
Arginine | - |
Lysine | - |
Ornithine | - |
Oxidase | - |
10%lactose | - |
传代数 | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 |
比活力(U/g) | 260 | 255 | 250 | 242 | 263 | 251 | 244 | 239 | 267 | 258 |
e.e.(%) | 99 | 99 | 99 | 99 | 99 | 99 | 99 | 99 | 99 | 99 |
pH | 4.9 | 5.3 | 6.2 | 6.9 | 7.7 | 8.3 | 8.6 | 9.1 |
R-扁桃酸浓度(mM) | 5.2 | 8.3 | 10.2 | 14.2 | 16.6 | 18.5 | 18.2 | 17.2 |
温度(℃) | 20 | 30 | 40 | 50 | 60 |
R-扁桃酸浓度(mM) | 12.5 | 18.6 | 18.5 | 16.2 | 14.6 |
菌体浓度(mg/ml) | 1 | 3 | 5 | 7 | 9 |
R-扁桃酸浓度(mM) | 7.2 | 12.5 | 18.6 | 18.6 | 18.6 |
菌体浓度(mg/ml) | 1 | 3 | 5 | 7 | 9 |
R-邻氯扁桃酸浓度(mM) | 6.8 | 10.1 | 14.3 | 15.4 | 15.3 |
Claims (6)
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CN2009101544844A CN101701243B (zh) | 2009-11-02 | 2009-11-02 | 生物催化法生产r-扁桃酸及其衍生物的方法 |
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CN2009101544844A CN101701243B (zh) | 2009-11-02 | 2009-11-02 | 生物催化法生产r-扁桃酸及其衍生物的方法 |
Publications (2)
Publication Number | Publication Date |
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CN101701243A true CN101701243A (zh) | 2010-05-05 |
CN101701243B CN101701243B (zh) | 2012-05-30 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102660470A (zh) * | 2012-04-13 | 2012-09-12 | 浙江工业大学 | 中华根瘤菌及其生物拆分外消旋α-羟酸生产手性α-羟酸 |
CN104357531A (zh) * | 2014-10-08 | 2015-02-18 | 王同俊 | R-邻氯扁桃酸及其酰基化合物的制备方法 |
CN104774825A (zh) * | 2015-03-23 | 2015-07-15 | 浙江工业大学 | 腈水解酶突变体及其应用 |
CN105505904A (zh) * | 2016-01-19 | 2016-04-20 | 浙江工业大学 | 腈水解酶突变体、基因、载体、工程菌及应用 |
-
2009
- 2009-11-02 CN CN2009101544844A patent/CN101701243B/zh active Active
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102660470A (zh) * | 2012-04-13 | 2012-09-12 | 浙江工业大学 | 中华根瘤菌及其生物拆分外消旋α-羟酸生产手性α-羟酸 |
CN102660470B (zh) * | 2012-04-13 | 2013-07-31 | 浙江工业大学 | 中华根瘤菌及其生物拆分外消旋α-羟酸生产手性α-羟酸 |
CN104357531A (zh) * | 2014-10-08 | 2015-02-18 | 王同俊 | R-邻氯扁桃酸及其酰基化合物的制备方法 |
CN104774825A (zh) * | 2015-03-23 | 2015-07-15 | 浙江工业大学 | 腈水解酶突变体及其应用 |
CN104774825B (zh) * | 2015-03-23 | 2017-11-07 | 浙江工业大学 | 腈水解酶突变体及其应用 |
CN105505904A (zh) * | 2016-01-19 | 2016-04-20 | 浙江工业大学 | 腈水解酶突变体、基因、载体、工程菌及应用 |
CN105505904B (zh) * | 2016-01-19 | 2019-02-01 | 浙江工业大学 | 腈水解酶突变体、基因、载体、工程菌及应用 |
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Publication number | Publication date |
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CN101701243B (zh) | 2012-05-30 |
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