CN101695286A - Juvenile turbot culturing and feeding method - Google Patents

Juvenile turbot culturing and feeding method Download PDF

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Publication number
CN101695286A
CN101695286A CN200910019286A CN200910019286A CN101695286A CN 101695286 A CN101695286 A CN 101695286A CN 200910019286 A CN200910019286 A CN 200910019286A CN 200910019286 A CN200910019286 A CN 200910019286A CN 101695286 A CN101695286 A CN 101695286A
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feeding
turbot
feed
throwing something
something
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郑珂珂
梁萌青
常青
方伟
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention relates to a juvenile turbot culturing and feeding method. Juvenile flounder conventional commercial feed is fed to a juvenile turbot with a size of 25-50g by 80 percent of feeding satiation level once at 15:00-18:00 every afternoon. The method can achieve the same growth effect of 3 to 4 times one day usually adopted in the traditional industrialized culture with the feeding satiation without influencing the immunity property of the juvenile turbot, and can lower the content of nitrite and ammonia nitrogen in the culture water environment. The turbot is fed once every day, which saves manpower and material resources, promotes the growth of the juvenile turbot, lowers about 20 percent of feed cost, improves nearly 100 percent of the feed efficiency, and can obviously improve the production benefit. The method is suitable for the industrialized culture of the juvenile turbot.

Description

Juvenile turbot culturing and feeding method
Technical field:
The invention belongs to the marine fish culture technology, is the feeding method that a kind of young turbot is cultured
Background technology:
Utilizing manufactured feed, carry out the fish intensive culture of high investment high production, is the more and more general production model of present aquaculture.In this aquaculture model, feed cost can account for more than 60% of aquaculture cost, and except feeding quality, the feeding technology of feed also has remarkable influence to cultured output and benefit.At present, feeding volume of feed (be satiated with food throw something and feed and limit food to throw something and feed) and the mode of throwing something and feeding (throw something and feed time and frequency) are to obtain according to raiser's experience for many years basically, and they lack the research of systematic science ground to the rational mode of throwing something and feeding.What have exists some mistaken ideas in aquaculture management, many raiser's excess are thrown something and fed, and think that such aquatic animal to grow is faster, and also the someone attempts to substitute to remedy with the excessive low quality feed of throwing something and feeding not use the high-quality feed.In fact; excessively throw something and feed and use the low quality feed can cause a lot of adverse consequencess; as feed spending is big, feed coefficient is high, increase water quality management difficulty, exacerbate water body pollution; increase the animal incidence of disease etc.; not only have influence on output and benefit, but also can bring the problem of environmental protection and healthy aquaculture.
Raising in the operation in concrete throwing, at first is determining of feeding volume.From a plurality of angles such as economic benefit and environmental protection sustainable developments, which kind of feed of no matter throwing something and feeding all is to pay the utmost attention to the forage volume of being thrown something and fed, and accomplishes the low high production that drops into, and obtains the best or maximum fish body weight gains.(Chinese Academy of Sciences's doctorate paper " utilizing the bioenergetics model to set up the throw something and feed research of system of hybridized prussian carp " such as aquatile research institute of Chinese Academy of Sciences Zhou Zhigang, 2002) hybridized prussian carp of setting up according to the suitableeest grazing rate is thrown something and fed and is shown obviously to save the feed consumption, improves feed efficiency.The management system of throwing something and feeding of fish also comprises determining of the time of throwing something and feeding and frequency.France scholar Gelineau (" Physiology andBehavior ", 1996) and Baras (" Cahiersd ' Ethologie Fondamentale etAppliquee, Animale et Humaine " 1995) etc. studies have shown that rainbow trout, Tilapia mossambica, the rhythm and pace of moving things of ingesting has appreciable impact to fish growth, make feeding activity every day of fish body be synchronized with most of somatic cell growing multiplication phases, can significantly improve aquatic products output and benefit; The rhythm and pace of moving things of ingesting of grasping fish can obviously improve efficiency of feed utilization.
Turbot is one of main mariculture kind of China.Its fast growth, easily domestication, acceptant mixed bait, suitable high-density breeding.But for the administrative skill of throwing something and feeding of turbot, the suitable feeding volume of system research turbot, the frequency of throwing something and feeding and time, yet there are no report both at home and abroad at present.
Summary of the invention:
The objective of the invention is to set up young turbot and culture the management method of reasonably throwing something and feeding, improve feed efficiency, reduce aquaculture cost, save China's feedstuff resource; Reduce fisheries environment and pollute, advance the sustainable sound development of culture fishery, for the turbot aquaculture management provides new scientific method.
The present invention finishes according to following method of operating:
Juvenile turbot culturing and feeding method: for the young turbot of specification at 25g-50g, every afternoon, the 15:00-18:00 point was thrown something and fed once.Feeding volume be satiated with food 80%, i.e. the 1.0%/d of young turbot body weight.
With the prior art contrast, the characteristics of the technology of the present invention are:
1. the feeding volume of young turbot, the frequency of throwing something and feeding and time have been carried out system research, from all angles such as growth performance, food utilization, immunologic function and cultivation water environment of young turbot, overall merit the influence of way to manage of throwing something and feeding to the turbot aquaculture.
2. 80% the level of throwing something and feeding of finding to be satiated with food does not influence the growth and the immune response of young turbot, and the close growth performance of throwing something and feeding can obtain and be satiated with food.
3. the suitable frequency of throwing something and feeding is 1 time/d, the turbot of throwing something and feeding once a day can reach with industrial aquaculture at present in the identical growth result of generally throwing something and feeding 3 to 4 times the every day of employing, use manpower and material resources sparingly.
4. 15:00 point-18:00 point was thrown something and fed and was thrown something and fed than other times section afternoon, can significantly promote the growth of young turbot, reduced by 20% feed cost, and it is nearly 100% that feed efficiency improves, and significantly improves productivity effect.
Embodiment:
Be described in detail the young turbot management method of throwing something and feeding below by embodiment.
Most preferred embodiment concrete operations technical process:
At first determine young turbot feed the best level of throwing something and feeding, growth performance, food utilization, immune performance by the horizontal group experiment fish of respectively throwing something and feeding reach the influence to breeding environment water quality, carry out Comprehensive Assessment, think that 80% of the level of being satiated with food of throwing something and feeding is the best level of throwing something and feeding, can reach and the close growth result of the level of being satiated with food of throwing something and feeding, do not influence the immune performance of young turbot, save feed, obtain higher feed efficiency, reduce feed cost, and significantly reduce nitrite nitrogen and ammonia nitrogen content in the cultivation water environment.
The mode of throwing something and feeding during young turbot is cultured: for the young turbot of specification 25g-50g, throw something and feed in the 15:00-18:00 point and once get final product every day.The turbot of throwing something and feeding once a day can reach with present industrial aquaculture in every day of generally adopting the identical growth result of throwing something and feeding 3 to 4 times, use manpower and material resources sparingly.15:00 point-18:00 point was thrown something and fed to throw something and feed than other times and was significantly promoted the growth of young turbot afternoon, improved feed efficiency and productivity effect, and helped desilting inspection before leaving offices in workman's afternoon, and feasibility is strong.
It is as follows to finish above-mentioned technical process concrete operations:
1. the best level of throwing something and feeding is definite: set 6 groups of different levels of throwing something and feeding, young turbot (average weight 29g) is carried out 45 days growth experiment by a definite date, throw something and feed respectively be satiated with food 100%, 90%, 80%, 70%, 60% and 50%, write down the feeding volume of every day, each level of throwing something and feeding is established three repetitions.The experimental session water temperature is 14-15 ℃.When growth experiment finishes, long situation of determination experiment sashimi (raw fish) and food utilization, the result is as shown in table 1; Get the experiment fish and take a blood sample, measure serum lysozyme, superoxide dismutase SOD, alkaline phosphatase, activity of acid phosphatase and TAC, the results are shown in Table 2.Experimental session is thrown something and fed level to the influence of breeding environment water quality for verifying difference, gathers water determination ammonia nitrogen, nitrite nitrogen and phosphate at twice in each experiment cylinder, the results are shown in Table 3.
The influence of level of throwing something and feeding of table 1 difference to young turbot growth and food utilization
The level of throwing something and feeding Whole last average weight g Rate of body weight gain % The heavy coefficient of variation of whole opisthosoma Grazing rate Feed efficiency
??100% ??51.5±1.3 a ??78.1±4.5 a ??21.0±0.8 a ??1.09±0.02 a ??113.9±3.1 a
??90% ??48.9±1.2 ab ??68.8±4.0 ab ??19.1±3.1 a ??0.97±0.01 b ??117.1±4.0 ab
The level of throwing something and feeding Whole last average weight g Rate of body weight gain % The heavy coefficient of variation of whole opisthosoma Grazing rate Feed efficiency
??80% ??48.9±1.2 ab ??68.9±4.2 ab ??18.9±1.1 a ??0.91±0.01 c ??124.5±6.7 ab
??70% ??47.5±1.2 bc ??64.3±4.2 bc ??14.8±1.9 ab ??0.83±0.01 d ??127.8±5.9 ab
??60% ??44.9±0.4 cd ??55.1±1.4 cd ??11.2±1.6 b ??0.73±0.00 e ??131.3±3.1 b
??50% ??42.6±0.2 d ??47.1±0.6 d ??16.2±2.3 ab ??0.64±0.00 f ??129.2±1.6 b
Annotate: rate of body weight gain (%)=(whole opisthosoma weighs-initial body weight) * 100/ initial body weight
Standard error/whole the opisthosoma of the every nautilus body weight of the heavy coefficient of variation=100* of whole opisthosoma is heavy
Grazing rate (% body weight/day)=100 * total solids food ration/[fate * (initial body weight+whole opisthosoma is heavy)/2]
Feed efficiency (%)=100 * fish body weight gains (weight in wet base)/total solids food ration
The influence of level of throwing something and feeding of table 2 difference to the young turbot immune indexes
The level of throwing something and feeding Lysozyme (ug/ml) Superoxide dismutase (U/ml) Alkaline phosphatase (King unit/ml) Acid phosphatase (U/ml) TAC (U/ml)
??100% ??10.52±0.02 ??100.21±2.51 ??2.43±0.14 ??2.28±0.02 a ??4.89±1.11 a
??90% ??10.12±0.38 ??96.34±2.91 ??2.91±0.28 ??2.05±0.16 ab ??4.89±0.88 a
??80% ??10.29±0.03 ??99.33±2.16 ??2.86±0.61 ??2.00±0.12 ab ??5.18±0.38 a
??70% ??10.64±0.04 ??98.54±2.52 ??2.94±0.27 ??1.54±0.11 b ??8.14±0.70 b
??60% ??10.54±0.08 ??98.45±2.05 ??2.63±0.07 ??1.73±0.25 ab ??6.74±0.29 ab
??50% ??10.60±0.07 ??97.40±2.24 ??2.32±0.15 ??1.59±0.27 b ??4.89±1.02 a
The influence of level of throwing something and feeding of table 3 difference to breeding environment water quality
The level of throwing something and feeding Nitrite nitrogen (mg/L) Ammonia nitrogen (mg/L) Phosphate (mg/L)
??100% ??0.019±0.001 a ??0.083±0.003 a ??0.084±0.008 a
??90% ??0.018±0.000 ab ??0.082±0.004 a ??0.073±0.005 a
The level of throwing something and feeding Nitrite nitrogen (mg/L) Ammonia nitrogen (mg/L) Phosphate (mg/L)
??80% ??0.014±0.002 bc ??0.073±0.001 ab ??0.077±0.001 a
??70% ??0.012±0.002 c ??0.070±0.006 b ??0.071±0.001 a
??60% ??0.010±0.001 c ??0.067±0.003 b ??0.055±0.003 b
??50% ??0.011±0.001 c ??0.053±0.002 c ??0.059±0.001 b
As can be seen from Table 1, along with the reduction of the level of throwing something and feeding, the whole opisthosoma of experiment fish weighs and rate of body weight gain shows downward trend.But 80%, 90% and 100% the young turbot of being satiated with food of throwing something and feeding, heavy and the whole last rate of body weight gain difference of whole opisthosoma is remarkable (p>0.05) not, the 70% experiment fish rate of body weight gain of throwing something and feeding horizontal group significantly is lower than 100% horizontal group (p<0.05) of throwing something and feeding, and 50% the throw something and feed experiment fish rate of body weight gain of horizontal group is minimum.The 60% heavy coefficient of variation of the whole opisthosoma of experiment fish of throwing something and feeding horizontal group significantly is lower than 80%, 90% and 100% horizontal group (p<0.05) of throwing something and feeding, all the other each groups do not have significant difference (p>0.05), the reduction of the level of throwing something and feeding can not bring the differentiation of young turbot individual growth, and the competition of ingesting of this fish is not fierce.The difference level of throwing something and feeding has appreciable impact to the feed efficiency of young turbot, decline along with the level of throwing something and feeding, the feed efficiency of experiment fish shows the trend of rising, and 50% and 60% the horizontal group experiment fish meal efficient of throwing something and feeding is significantly higher than 100% horizontal group (p<0.05) of throwing something and feeding.
Table 2 has been listed the different influences of level to the young turbot immune indexes of throwing something and feeding.The difference level of throwing something and feeding has no significant effect (p>0.05) to turbot serum lysozyme, superoxide dismutase and alkaline phosphatase.Reduction along with the level of throwing something and feeding, the turbot PAS shows the trend (p<0.05) of reduction, 80% to the 100% group difference not significantly (p>0.05) of throwing something and feeding, and the 70% and 50% group experiment fish serum phosphatase of throwing something and feeding significantly is lower than 100% group (p<0.05) of throwing something and feeding.TAC shows the trend (p<0.05) that raises and afterwards reduce earlier.
As can be seen from Table 3, the difference young turbot breeding environment water quality index of having thrown something and fed horizontal appreciable impact.Along with the reduction of the level of throwing something and feeding, the nitrite nitrogen in the cultivation water environment, ammonia nitrogen and phosphate all significantly reduce (p<0.05).80% nitrite nitrogen of throwing something and feeding horizontal group significantly is lower than 100% horizontal group (p<0.05) of throwing something and feeding, and does not have notable difference (p>0.05) with 50% to 70% horizontal group of throwing something and feeding.50% to 70% throw something and feed horizontal group nitrite nitrogen and ammonia-nitrogen content all significantly is lower than 90% and 100% horizontal group (p<0.05) of throwing something and feeding.50% and the 60% horizontal group phosphate content of throwing something and feeding significantly is lower than 70% to 100% horizontal group (p<0.05) of throwing something and feeding, and it is not remarkable that other respectively organizes difference.
In sum, 80% (1.0%/d that is equivalent to body weight) of the level of being satiated with food of throwing something and feeding can reach and close growth result of the level of being satiated with food and the higher feed efficiency of throwing something and feeding, and reduces feed cost, significantly reduces nitrite nitrogen and ammonia nitrogen content in the cultivation water environment.
2. the suitable frequency of throwing something and feeding is definite: set 3 groups of different frequencies of throwing something and feeding, throw something and feed every day 1 time (8:00), 2 (8:00; 17:00) with 3 (8:00; 12:00; 17:00), young turbot (average weight 29g) is carried out 45 days growth experiment by a definite date, write down the feeding volume of every day, each frequency of throwing something and feeding is established three repetitions.The experimental session water temperature is 14-15 ℃.When growth experiment finishes, long situation of determination experiment sashimi (raw fish) and food utilization, the result is as shown in table 4.
Table 4 difference is thrown something and fed, and frequency is grown to young turbot and the influence of food utilization
The frequency of throwing something and feeding The heavy g of whole opisthosoma Rate of body weight gain % Grazing rate Feed efficiency
1 time/d ??49.8±0.3 ??72.5±1.1 ??1.03±0.02 ??115.2±1.0
2 times/d ??50.7±2.1 ??75.2±7.6 ??1.05±0.03 ??114.9±5.1
3 times/d ??52.0±2.6 ??80.3±9.0 ??1.08±0.06 ??117.4±3.7
Annotate: rate of body weight gain (%)=(whole opisthosoma weighs-initial body weight) * 100/ initial body weight
Grazing rate (% body weight/day)=100 * total solids food ration/[fate * (initial body weight+whole opisthosoma is heavy)/2]
Feed efficiency (%)=100 * fish body weight gains (weight in wet base)/total solids food ration
The influence of frequency of throwing something and feeding of table 5 difference to the young turbot immune indexes
The frequency of throwing something and feeding Lysozyme (ug/ml) Superoxide dismutase (U/ml) Alkaline phosphatase (King unit/ml) Acid phosphatase (U/ml) TAC (U/ml)
1 time/d ??10.41±0.28 ??94.49±3.82 ??1.81±0.37 ??1.85±0.42 ??6.66±2.33
2 times/d ??10.61±0.15 ??95.29±1.10 ??2.15±0.14 ??1.95±0.25 ??6.33±0.89
3 times/d ??10.39±0.13 ??92.65±1.65 ??1.76±0.16 ??1.92±0.31 ??5.76±1.37
As shown in table 4, the different frequencies of throwing something and feeding (1-3 time/d) growth, grazing rate and the feed efficiency to young turbot all has no significant effect (p>0.05).Turbot is that the stomach fish is arranged, and observes in the experiment and can find out that it belongs to the slower fish of gastric emptying, the suitable capacity bait of taking in once a day, and the defecation peak appears at ingests back about 10 to 12 hours.The difference frequency of throwing something and feeding all has no significant effect (table 5) to immune indexes such as turbot serum lysozyme, SOD, alkaline phosphatases.Therefore, in the aquaculture management, the turbot in this stage is thrown something and fed and once promptly reach and the identical growth result of throwing something and feeding 2 to 3 times every day, can use manpower and material resources sparingly.
3. the screening of the suitable time of throwing something and feeding: adopt the rhythm method of ingesting round the clock of throwing something and feeding of once being satiated with food, diel is divided into eight periods, each period, three were repeated cylinder as a processing horizontal, and every day, each experiment cylinder was thrown something and fed once.Eight processing are respectively: 6:00,9:00,12:00,15:00,18:00,21:00,0:00 and 3:00 throw something and feed, totally 24 experiment cylinders.It is the young turbot of 27g that each experiment cylinder is put into the initial average weight of 20 tails, carries out 7 days growth experiment by a definite date, and the experimental session water temperature is 14-15 ℃.
Table 6 influence of time of throwing something and feeding to young turbot growth and food utilization
Throw something and feed the time The heavy g of whole opisthosoma Specific growth rate (%/d) Grazing rate Feed efficiency
??6:00 ??27.19±1.02 a ??0.90±0.07 a ??0.86±0.08 a ??96.65±8.84 a
Throw something and feed the time The heavy g of whole opisthosoma Specific growth rate (%/d) Grazing rate Feed efficiency
??9:00 ??28.21±0.10 a ??0.87±0.16 a ??1.02±0.03 abc ??84.19±13.74 a
??12:00 ??28.03±0.37 a ??0.80±0.18 a ??0.93±0.08 ab ??85.07±13.29 a
??15:00 ??30.22±0.91 c ??2.09±0.42 c ??1.13±0.12 bcd ??180.66±19.03 b
??18:00 ??31.14±0.41 bc ??1.76±0.33 bc ??1.00±0.07 abc ??179.01±40.82 b
??21:00 ??29.17±0.95 ab ??1.20±0.21 ab ??1.18±0.06 cd ??101.03±16.60 a
??0:00 ??28.98±0.64 ab ??1.15±0.19 ab ??1.12±0.08 bcd ??102.60±12.57 a
??3:00 ??30.84±0.52 bc ??1.81±0.16 bc ??1.28±0.03 d ??140.84±13.00 ab
As shown in table 6, difference the throw something and feed growth and the food utilization (p<0.05) of time appreciable impact young turbot.The throw something and feed growth of group young turbot of 15:00 point is best, and feed efficiency is also the highest, all is significantly higher than 6:00,9:00,12:00,21:00 and the 0:00 group (p<0.05) of throwing something and feeding.The throw something and feed specific growth rate of group experiment fish and feed efficiency of 15:00 point is morning 6:00,9:00 and throw something and feed about 2 times of each group of 12:00.15:00,18:00,3:00 point throw something and feed group experiment fish specific growth rate and feed efficiency difference not significantly (p>0.05).Therefore, actual in conjunction with producing, the suitable time of throwing something and feeding of young turbot is this time period of 15:00-18:00.
4. the application of feeding technology: Tian Yuan aquatic products Co., Ltd has carried out application experiment in Yantai, and specification is half two young turbot, and every afternoon, the 15:00 point was thrown something and fed once.The level of throwing something and feeding is 80% (1.0%/d that is equivalent to body weight) that is satiated with food.Experimental session DO>6.0mg/L, pH are 7.5-8.0,14 ± 1 ℃ of water temperatures, salinity 20-22g L -1Behind one month by a definite date the growth experiment, experiment pool is compared with the breed pond (be satiated with food every day and throw something and feed 4 times) of other daily managements, and turbot growth does not have significant difference, and it is about 30% to save manufactured feed, and it is nearly 100% that feed efficiency improves, and aquaculture cost significantly reduces; Breeding water body ammonia nitrogen, nitrite nitrogen and phosphate content obviously reduce, and have reduced the pollution to environment; Daily management and clear pond workload obviously reduce, and a workman can manage more breed pond, has effectively saved manpower and materials.

Claims (1)

1. juvenile turbot culturing and feeding method, it is characterized in that its feeding method: for the young turbot of specification at 25g-50g, every afternoon, the 15:00-18:00 point was thrown something and fed once; Feeding volume be satiated with food 80%, i.e. the 1.0%/d of young turbot body weight.
CN200910019286A 2009-10-13 2009-10-13 Juvenile turbot culturing and feeding method Pending CN101695286A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101933480A (en) * 2010-08-31 2011-01-05 华南理工大学 Water-saving marine fish seedling culture method
CN103385194A (en) * 2013-08-02 2013-11-13 青岛七好生物科技有限公司 Large-scale efficient cultivation method for turbot
CN103749369A (en) * 2014-02-19 2014-04-30 青岛农业大学 Turbot and platichthys stellatus crossbreeding method
CN113632750A (en) * 2021-08-12 2021-11-12 大连智慧渔业科技有限公司 Feed feeding method for whole growth cycle of industrial culture of turbots
CN114208729A (en) * 2021-12-07 2022-03-22 中国水产科学研究院黄海水产研究所 Fugu rubripes gastrointestinal emptying parameter evaluation method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101933480A (en) * 2010-08-31 2011-01-05 华南理工大学 Water-saving marine fish seedling culture method
CN101933480B (en) * 2010-08-31 2012-10-31 华南理工大学 Water-saving marine fish seedling culture method
CN103385194A (en) * 2013-08-02 2013-11-13 青岛七好生物科技有限公司 Large-scale efficient cultivation method for turbot
CN103749369A (en) * 2014-02-19 2014-04-30 青岛农业大学 Turbot and platichthys stellatus crossbreeding method
CN113632750A (en) * 2021-08-12 2021-11-12 大连智慧渔业科技有限公司 Feed feeding method for whole growth cycle of industrial culture of turbots
CN114208729A (en) * 2021-12-07 2022-03-22 中国水产科学研究院黄海水产研究所 Fugu rubripes gastrointestinal emptying parameter evaluation method

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Open date: 20100421