CN114208729A - Fugu rubripes gastrointestinal emptying parameter evaluation method - Google Patents
Fugu rubripes gastrointestinal emptying parameter evaluation method Download PDFInfo
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- 241001441722 Takifugu rubripes Species 0.000 title claims abstract description 91
- 230000002496 gastric effect Effects 0.000 title claims abstract description 31
- 238000011156 evaluation Methods 0.000 title claims abstract description 10
- 241000251468 Actinopterygii Species 0.000 claims abstract description 52
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 238000000034 method Methods 0.000 claims abstract description 18
- 239000013535 sea water Substances 0.000 claims description 16
- 235000013305 food Nutrition 0.000 claims description 15
- 210000004185 liver Anatomy 0.000 claims description 10
- 238000004062 sedimentation Methods 0.000 claims description 10
- 210000000936 intestine Anatomy 0.000 claims description 9
- 230000000366 juvenile effect Effects 0.000 claims description 9
- 238000005070 sampling Methods 0.000 claims description 9
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- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 6
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 5
- 210000001835 viscera Anatomy 0.000 claims description 5
- 206010002091 Anaesthesia Diseases 0.000 claims description 4
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- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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- G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
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Abstract
The invention discloses a method for evaluating gastrointestinal emptying parameters of takifugu rubripes, which relates to the technical field of aquaculture and comprises the following steps: a1, selecting experimental fish: the invention randomly selects 3000 tails of large-size fugu rubripes fries without harm to health, carries out detailed evaluation on gastrointestinal emptying modes of the fugu rubripes fries by setting a comprehensive evaluation method, develops research on gastrointestinal emptying modes with different feeding frequencies under the condition of a small circulating water system, and aims to establish an optimal mathematical model which accords with gastrointestinal emptying characteristics of the large-size fugu rubripes fries, thereby solving the problem that research data on the gastrointestinal emptying modes of the large-size fries stage are not reported yet, further effective parameters cannot be provided for fugu rubripes culturists, further certain troubles are brought to the corresponding fugu rubripes culturists to set feeding strategies, and further great difficulty is brought to early cultivation of the large-size fugu rubripes fries.
Description
Technical Field
The invention relates to an aquaculture evaluation method, in particular to a takifugu rubripes gastrointestinal emptying parameter evaluation method.
Background
The takifugu rubripes is an important mariculture fish in China, is a fish in a warm temperate zone and a tropical offshore bottom layer, is delicious in meat flavor and rich in nutrition, is a rare economic fish variety, and in recent years, due to the release of the market of the takifugu rubripes, the culture scale is continuously increased, the culture mode is diversified, the culture yield is improved year by year, the national finished fish yield reaches about 8000 tons, the production value is 6 yuan, the takifugu rubripes is formed in a large scale, and has obvious economic benefit and social benefit, the current main culture mode of the takifugu rubripes is factory circulating water culture, in an intensive culture environment represented by factory circulating water culture, most nutrition and energy of aquaculture animals are sourced from input baits, the ingestion level and the bait utilization efficiency of the aquatic animals can be effectively improved by reasonably feeding the bait feeding strategy, the culture efficiency can be improved and the feed cost can be reduced by optimizing and adjusting the feeding strategy in a specific growth stage of the aquatic animals, the reasonable feed feeding needs to know the gastrointestinal emptying rate of the fish, the gastrointestinal emptying rate of the fish refers to the speed of the fish which is discharged from the stomach and the intestines after the fish ingests food, and is an important parameter for evaluating the digestion physiological function and the food digestibility of the fish, the recovery of the appetite of the fish is closely related to the gastric emptying rate and the emptying of the intestinal tracts, the research on the gastrointestinal emptying of the fish can help estimate the feeling of fullness after meals, so that the appetite recovery time of the fish can be evaluated, the feeding in the appetite peak period can not only improve the growth speed and the food conversion efficiency, but also furthest reduce the food waste, but at present, research data about the gastrointestinal emptying mode of the takifugu rubripes, in particular to the large-specification fry period, are not reported yet, effective parameters can not be provided for takifugu rubripes culturis, and the corresponding takifugu rubripes culturis are made to a certain trouble, therefore, great difficulty is brought to early culture of large-size fries of the fugu rubripes, and the invention provides the method for evaluating the gastrointestinal emptying parameters of the fugu rubripes to solve the problem.
Disclosure of Invention
The invention aims to provide a method for evaluating gastrointestinal emptying parameters of takifugu rubripes, which aims to solve the problems that the existing research data about the gastrointestinal emptying modes of takifugu rubripes in the background technology, especially in large-specification fry stages are not reported yet, and further effective parameters cannot be provided for takifugu rubripes culturists, and further certain troubles are brought to the corresponding takifugu rubripes culturists for formulating feeding strategies, so that great difficulty is brought to the early-stage cultivation of large-specification fries of takifugu rubripes.
In order to achieve the purpose, the invention provides the following technical scheme: a takifugu rubripes gastrointestinal emptying parameter evaluation method comprises the following steps:
a1, selecting experimental fish: randomly selecting 3000 tails of healthy and non-injurious large-size fugu rubripes seedlings, temporarily culturing the selected experimental fish in 4 sets of small circulating water systems respectively, feeding special feed for the fugu rubripes during temporary culture, feeding the feed according to 2.5% of the body weight, timely cleaning and removing residual bait and excrement after feeding for half an hour, wherein the illumination period is 12L to 12D, the culture water is selected from natural seawater treated by a sedimentation tank, and after temporary culture for one week, the healthy and non-injurious fish are randomly selected for experiment;
a2, grouping comparison: dividing experimental feeding gradients into four groups, namely continuous feeding, 2 times/day, 4 times/day and 6 times/day, randomly taking 200 takifugu rubripes juvenile fishes from each barrel, setting 3 gradients in parallel, carrying out 2.5% quantitative feeding according to the feeding strategy during a culture test period, counting the residual baits in each culture barrel after feeding for 0.5h, wherein the illumination period is 12L:12D in the grouped contrast culture process, the culture water is selected from natural seawater treated by a sedimentation tank, the pH of the seawater is 7.5, the dissolved oxygen is 6.5mg/L, the salinity is 32, the temperature is 20 +/-1.0 ℃, the nitrite dissolved concentration is less than or equal to 0.1mg/L, and the ammonia nitrogen solubility is less than or equal to 0.2 mg/L;
a3, data acquisition: after the culture experiment is finished in 60 days, feeding by satiation is started at 6 points in the morning until no fish eats after the feed of each treatment group sinks for 1-2 minutes, at the moment, the treatment group is deemed to be satiated, continuous sampling is carried out according to the sampling time shown in the figure 1 after the satiation is finished, 5 samples are taken each time, the ice tray is dissected after MS-222 anesthesia, the body length, the full length, the body weight, the visceral weight and the liver weight of the fish are all measured, and then the gastrointestinal food mass is scraped and accurately weighed in an electronic balance, wherein the length is accurate to 1mm, and the weight is accurate to 0.1 g;
a4, obtaining the result: the following five data were obtained from the growth assay and corresponding calculations: the method comprises the following steps of 1, evaluating the growth performance of the takifugu rubripes by different feeding frequencies, 2, influencing filling and emptying of the takifugu rubripes by different feeding frequencies, 3, influencing emptying rate of the takifugu rubripes by different feeding frequencies, 4, influencing filling and emptying of the takifugu rubripes intestines by different feeding frequencies, and 5, obtaining the feeding mode most suitable for the takifugu rubripes juvenile fish at the current stage through the five data.
The error of the selected weight of the experimental fish in a1 is 82.36 +/-2.62 g, 4 sets of small circulating water systems contained in a1 comprise 12 circular water tanks, and each set of the 4 sets of small circulating water systems has the length of 3m3The water body in the a1 is the culture water selected from natural seawater treated by a sedimentation tank, and the culture water meets the following standards: the pH value of the seawater is 7.5, the dissolved oxygen is 6.5mg/L, the salinity is 32, the temperature is 20 +/-1.0 ℃, the dissolved concentration of nitrite is less than or equal to 0.1mg/L, and the solubility of ammonia nitrogen is less than or equal to 0.2 mg/L.
In a preferred embodiment of the present invention, the growth measurement method is growth index measurement, and the growth index measurement includes the following data: survival rate, i.e. survivval, is equal to 100 times the number of final or initial fish, absolute growth rate, i.e. SGR, is equal to 100 times the difference between the last ln weight and the first ln weight divided by the cultivation time, feed factor, i.e. FCR, is equal to consumed feed divided by the weight gain, fullness, i.e. CF, is equal to W divided by L3 and multiplied by 100, liver index, i.e. HIS,% is equal to liver weight divided by the total fish weight multiplied by 100, and viscera ratio, i.e. VSI,% is equal to viscera weight divided by the total fish weight multiplied by 100.
Compared with the prior art, the invention has the beneficial effects that:
the invention makes detailed evaluation on the gastrointestinal emptying mode of the fugu rubripes fries by setting a comprehensive evaluation method, develops research on the gastrointestinal emptying mode with different feeding frequencies under the condition of a small circulating water system, aims to establish an optimal mathematical model conforming to the gastrointestinal emptying characteristics of large-scale fries of the fugu rubripes, judges the appetite recovery condition of the fed fugu rubripes based on the mathematical model, provides reasonable suggestions for the intelligent feeding of industrial circulating water feed of the fugu rubripes, provides technical support for the early cultivation of the large-scale fries of the fugu rubripes, and research results made by the method are also used for optimizing reasonable feeding strategies for cultivated fishes, so the method is particularly suitable for an industrial cultivation system under an automatic feeding mode, thereby solving the problem that research data on the gastrointestinal emptying mode of the fugu rubripes, particularly in the large-scale fries stage is not reported yet, and further cannot provide effective parameters for the fugu rubripes culturis, and then certain trouble is brought to the formulation of the feeding strategy for the corresponding fugu rubripes culturists, so that the problem of great difficulty is brought to the early cultivation of large-size fugu rubripes seedlings.
Drawings
FIG. 1 is a schematic structural view of different feeding times and sampling times of the present invention;
FIG. 2 is a schematic diagram showing the effect of different feeding frequencies on the gastrointestinal emptying of Fugu rubripes according to the present invention;
FIG. 3 is a schematic structural diagram of a fugu rubripes gastric emptying rate index model under different feeding frequency conditions;
FIG. 4 is a schematic diagram showing the effect of different feeding frequencies on the filling and emptying of the Fugu rubripes intestine according to the present invention;
fig. 5 is a schematic diagram of the residence time structure of the takifugu rubripes intestinal tract food under different feeding frequency conditions.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides a method for evaluating gastrointestinal emptying parameters of takifugu rubripes, comprising the following steps:
the first embodiment is as follows:
firstly, selecting experimental fish: 3000 tails of healthy and non-injurious large-size fugu rubripes fries are randomly selected, then the selected experimental fish are temporarily cultured in 4 sets of small circulating water systems respectively, special feed for the fugu rubripes is fed during the temporary culture, the fed feed is fed according to 2.5% of the body weight, the fed nutrient composition table is shown in table I, residual bait and excrement are timely cleaned and removed after the fed fish are fed for half an hour, the illumination period is 12L:12D, the culture water is selected from natural seawater treated by a sedimentation tank, and after the temporary culture is carried out for one week, the healthy and non-injurious fish are randomly selected for experiment.
TABLE 1 nutritional ingredients of experimental feeds
Grouping comparison is carried out after: dividing experimental feeding gradients into four groups, namely continuous feeding, 2 times/day, 4 times/day and 6 times/day, randomly taking 200 juvenile takifugu rubripes from each barrel, arranging 3 gradients in parallel, carrying out 2.5% quantitative feeding according to the feeding strategy during a culture test period, counting the residual baits in each culture barrel after feeding for 0.5h, wherein the illumination period is 12L:12D in the grouped contrast culture process, the culture water is selected from natural seawater treated by a sedimentation tank, the pH of the seawater is 7.5, the dissolved oxygen is 6.5mg/L, the salinity is 32, the temperature is 20 +/-1.0 ℃, the nitrite dissolved concentration is less than or equal to 0.1mg/L, and the ammonia nitrogen solubility is less than or equal to 0.2 mg/L.
Then data acquisition is carried out: after the culture experiment for 60 days is finished, feeding by satiation is started at 6 am until no fish eat at the bottom of feed of each treatment group for 1-2 minutes, at the moment, the treatment group is deemed to be satiated, continuous sampling is carried out according to the sampling time shown in figure 1 after the satiation is finished, 5 samples are taken each time, the ice tray is dissected after MS-222 anesthesia, the body length, the full length, the body weight, the visceral weight and the liver weight of the fish are all measured, and then the food mass in the stomach and intestine is scraped and accurately weighed in an electronic balance, wherein the length is accurate to 1mm, and the weight is accurate to 0.1 g.
Further, the following results are obtained: the following five data were obtained from the growth assay and corresponding calculations: 1, evaluating the growth performance of the takifugu rubripes by different feeding frequencies: after the fugu rubripes fries are cultured at different feeding frequencies for 60 days, the growth indexes are shown in table 2, the specific growth rate and survival rate of the terminal weight and the weight gain rate of the fugu rubripes are the highest, the feed coefficient is the lowest, the fugu rubripes are fed for six groups, the specific growth rate and survival rate of the terminal weight and the weight gain rate of the continuous feeding group are the lowest, the feed coefficient is the highest, namely P is more than 0.05, different feeding frequencies have certain influence on the liver body ratio of the fugu rubripes, wherein the liver body ratio of the fugu rubripes fed for four times and six times is obviously higher than that of the fugu rubripes fed for two times, namely P is less than 0.05, and in addition, different feeding frequencies have no obvious influence on the viscera ratio and fatness of the fugu rubripes, namely P is more than 0.05,
TABLE 2 influence of different feeding frequencies on growth performance of Fugu rubripes
Note: data are presented as mean ± standard deviation. Different letters after the results indicate significant differences between groups, i.e. P < 0.05.
Effect of different feeding frequencies on gastric filling and emptying of takifugu rubripes: as can be seen from figure 2, the gastrointestinal emptying modes and the gastrointestinal emptying times of the takifugu rubripes do not have obvious difference in different feeding frequencies, wherein the initial feeding relative content of each feeding group of the takifugu rubripes is respectively 4.86 +/-0.15, 4.76 +/-0.28, 4.51 +/-0.22 and 4.71 +/-0.17, the relative content in the stomach after 4 hours is greatly reduced to respectively reach 2.55 +/-0.13, 2.69 +/-0.21, 2.97 +/-0.16 and 2.77 +/-0.19, the relative content of the residual food in the stomach after 8 hours is basically digested to completion, and is respectively 0.23 +/-0.02, 0.21 +/-0.01, 0.31 +/-0.02 and 0.57 +/-0.09, and the digestion completion is completed within 10-12 hours.
The influence of different feeding frequencies on the gastric emptying rate of the takifugu rubripes: the fugu rubripes gastric emptying rate model is fitted by an exponential model, as can be seen from fig. 3, different feeding frequencies have no significant influence on the fugu rubripes gastric emptying rate model, and the content of food in the stomach gradually decreases with the time, and as can be seen from table 5, different feeding frequencies have no significant influence on the fugu rubripes gastric emptying rate, the gastric emptying rate under the normal condition is 5.11 +/-0.09 mg 100gBW min-1, and the fugu rubripes can digest food in the stomach to about 50% in about 188.74 +/-12.83 min after being saturated.
Effect of different feeding frequencies on filling and emptying of the fugu rubripes intestine: as can be seen from fig. 4, the filling and emptying patterns and the time variation of the intestines of each feeding group are similar and have little difference, wherein the relative filling content in the intestines of each feeding group is gradually increased along with the prolonging of the feeding time and the digestion time, the intestinal canal filling amount is kept at a higher level within 3-6 hours of the full eating, then the intestinal emptying is accelerated, the emptying speed is greatly increased from 6-10 hours, the intestinal canal is basically emptied within 12 hours, then the filling and emptying of the intestinal canal are subjected to sectional fitting, and calculating the main residence time of the food in the intestinal tract based on 50% of the filling degree and 50% of the emptying rate of the intestinal tract, as can be seen from fig. 5, different feeding frequencies do not influence the residence time of the food in the intestinal tract, the residence time of the food in the intestinal tract is basically calculated to be 5.80 +/-0.29 hours, and the data as shown in the following table 3 are calculated.
The residence time of the takifugu rubripes intestinal food under different feeding frequency conditions is shown in fig. 5.
And then the feeding mode which is most suitable for the juvenile fish of the takifugu rubripes at the current stage is obtained through the five data, namely the fourth feeding mode in the test is the feeding mode which is most suitable for the juvenile fish of the takifugu rubripes at the current stage.
TABLE 3 influence of different feeding frequency on gastric emptying rate and emptying time of Fugu rubripes
When in specific use, the following steps are required:
s1, selecting experimental fish: randomly selecting 3000 tails of healthy and non-injurious large-size fugu rubripes seedlings, temporarily culturing the selected experimental fish in 4 sets of small circulating water systems respectively, feeding special feed for the fugu rubripes during temporary culture, feeding the feed according to 2.5% of the body weight, timely cleaning and removing residual bait and excrement after feeding for half an hour, wherein the illumination period is 12L to 12D, the culture water is selected from natural seawater treated by a sedimentation tank, and after temporary culture for one week, the healthy and non-injurious fish are randomly selected for experiment;
s2, grouping comparison: dividing experimental feeding gradients into four groups, namely continuous feeding, 2 times/day, 4 times/day and 6 times/day, randomly taking 200 takifugu rubripes juvenile fishes from each barrel, setting 3 gradients in parallel, carrying out 2.5% quantitative feeding according to the feeding strategy during a culture test period, counting the residual baits in each culture barrel after feeding for 0.5h, wherein the illumination period is 12L:12D in the grouped contrast culture process, the culture water is selected from natural seawater treated by a sedimentation tank, the pH of the seawater is 7.5, the dissolved oxygen is 6.5mg/L, the salinity is 32, the temperature is 20 +/-1.0 ℃, the nitrite dissolved concentration is less than or equal to 0.1mg/L, and the ammonia nitrogen solubility is less than or equal to 0.2 mg/L;
s3, data acquisition: after the culture experiment is finished in 60 days, feeding by satiation is started at 6 points in the morning until no fish eats after the feed of each treatment group sinks for 1-2 minutes, at the moment, the treatment group is deemed to be satiated, continuous sampling is carried out according to the sampling time shown in the figure 1 after the satiation is finished, 5 samples are taken each time, the ice tray is dissected after MS-222 anesthesia, the body length, the full length, the body weight, the visceral weight and the liver weight of the fish are all measured, and then the gastrointestinal food mass is scraped and accurately weighed in an electronic balance, wherein the length is accurate to 1mm, and the weight is accurate to 0.1 g;
s4, obtaining a result: the following five data were obtained from the growth assay and corresponding calculations: the method comprises the following steps of 1, evaluating the growth performance of the takifugu rubripes by different feeding frequencies, 2, influencing filling and emptying of the takifugu rubripes by different feeding frequencies, 3, influencing emptying rate of the takifugu rubripes by different feeding frequencies, 4, influencing filling and emptying of the takifugu rubripes intestines by different feeding frequencies, and 5, obtaining the feeding mode most suitable for the takifugu rubripes juvenile fish at the current stage through the five data.
In the description of the present invention, it is to be understood that the indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings and are only for convenience in describing the present invention and simplifying the description, but are not intended to indicate or imply that the indicated devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be construed as limiting the present invention.
In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly attached, detachably attached, or integrated; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate, and may be communication between two elements or interaction relationship between two elements, unless otherwise specifically limited, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to specific situations.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. A takifugu rubripes gastrointestinal emptying parameter evaluation method comprises the following steps:
a1, selecting experimental fish: randomly selecting 3000 tails of healthy and non-injurious large-size fugu rubripes seedlings, temporarily culturing the selected experimental fish in 4 sets of small circulating water systems respectively, feeding special feed for the fugu rubripes during temporary culture, feeding the feed according to 2.5% of the body weight, timely cleaning and removing residual bait and excrement after feeding for half an hour, wherein the illumination period is 12L to 12D, the culture water is selected from natural seawater treated by a sedimentation tank, and after temporary culture for one week, the healthy and non-injurious fish are randomly selected for experiment;
a2, grouping comparison: dividing experimental feeding gradients into four groups, namely continuous feeding, 2 times/day, 4 times/day and 6 times/day, randomly taking 200 takifugu rubripes juvenile fishes from each barrel, setting 3 gradients in parallel, carrying out 2.5% quantitative feeding according to the feeding strategy during a culture test period, counting the residual baits in each culture barrel after feeding for 0.5h, wherein the illumination period is 12L:12D in the grouped contrast culture process, the culture water is selected from natural seawater treated by a sedimentation tank, the pH of the seawater is 7.5, the dissolved oxygen is 6.5mg/L, the salinity is 32, the temperature is 20 +/-1.0 ℃, the nitrite dissolved concentration is less than or equal to 0.1mg/L, and the ammonia nitrogen solubility is less than or equal to 0.2 mg/L;
a3, data acquisition: after the culture experiment is finished in 60 days, feeding by satiation is started at 6 points in the morning until no fish eats after the feed of each treatment group sinks for 1-2 minutes, at the moment, the treatment group is deemed to be satiated, continuous sampling is carried out according to proper sampling time after the satiation is finished, 5 samples are taken each time, the fish is dissected on an ice tray after MS-222 anesthesia, the body length, the full length, the body weight, the visceral weight and the liver weight of the fish are all measured, and then the gastrointestinal food mass is scraped and accurately weighed in an electronic balance, wherein the length is accurate to 1mm, and the weight is accurate to 0.1 g;
a4, obtaining the result: the following five data were obtained from the growth assay and corresponding calculations: the method comprises the following steps of 1, evaluating the growth performance of the takifugu rubripes by different feeding frequencies, 2, influencing filling and emptying of the takifugu rubripes by different feeding frequencies, 3, influencing emptying rate of the takifugu rubripes by different feeding frequencies, 4, influencing filling and emptying of the takifugu rubripes intestines by different feeding frequencies, and 5, obtaining the feeding mode most suitable for the takifugu rubripes juvenile fish at the current stage through the five data.
2. The method of claim 1, wherein the assessment method of gastrointestinal emptying parameters of takifugu rubripes comprises: the error of the selected weight of the experimental fish in the a1 is 82.36 +/-2.62 g, the 4 sets of small circulating water systems contained in the a1 comprise 12 circular water tanks, and each set of the 4 sets of small circulating water systems has the length of 3m3The water body in the a1 is the culture water selected from natural seawater treated by a sedimentation tank, and the culture water meets the following standards: the pH value of the seawater is 7.5, the dissolved oxygen is 6.5mg/L, the salinity is 32, the temperature is 20 +/-1.0 ℃, the dissolved concentration of nitrite is less than or equal to 0.1mg/L, and the solubility of ammonia nitrogen is less than or equal to0.2mg/L。
3. The method of claim 1, wherein the assessment method of gastrointestinal emptying parameters of takifugu rubripes comprises: the growth determination method is growth index determination, and the growth index determination comprises the following data: survival rate, i.e. survivval, is equal to 100 times the number of final or initial fish, absolute growth rate, i.e. SGR, is equal to 100 times the difference between the last ln weight and the first ln weight divided by the cultivation time, feed factor, i.e. FCR, is equal to consumed feed divided by the weight gain, fullness, i.e. CF, is equal to W divided by L3 and multiplied by 100, liver index, i.e. HIS,% is equal to liver weight divided by the total fish weight multiplied by 100, and viscera ratio, i.e. VSI,% is equal to viscera weight divided by the total fish weight multiplied by 100.
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