CN101693745B - Polysaccharides and their method of extraction of Ganoderma lucidum, Ganoderma lucidum polysaccharides added cigarette and preparation method - Google Patents

Polysaccharides and their method of extraction of Ganoderma lucidum, Ganoderma lucidum polysaccharides added cigarette and preparation method Download PDF

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CN101693745B
CN101693745B CN 200910189846 CN200910189846A CN101693745B CN 101693745 B CN101693745 B CN 101693745B CN 200910189846 CN200910189846 CN 200910189846 CN 200910189846 A CN200910189846 A CN 200910189846A CN 101693745 B CN101693745 B CN 101693745B
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ganoderma lucidum
polysaccharides
tobacco
cigarette
ganoderma
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CN101693745A (en
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温玉兴
温纯青
温育青
钟永楚
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广东神农烟科技术有限公司
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Abstract

本发明公开了一种灵芝多糖及其提取方法、添加灵芝多糖的卷烟及其制备方法,要解决的技术问题是降低卷烟中的有害成分。 The present invention discloses a method of extraction of Ganoderma lucidum polysaccharides and adding cigarette polysaccharides from its preparation method, the technical problem to be solved is to reduce the harmful components of cigarette. 灵芝多糖用下述方法得到:将灵芝菌体烘干,粉碎,在热水中浸提,过滤,减压浓缩,加入乙醇,静置,离心处理,过滤。 Ganoderma lucidum polysaccharides obtained by the following method: Ganoderma lucidum cells drying, grinding, extraction in hot water, filtered, and concentrated under reduced pressure, ethanol was added, standing, centrifugation, filtration. 添加灵芝多糖的卷烟,烟丝中含有占烟丝质量灵芝多糖。 Add Ganoderma lucidum polysaccharides cigarettes, tobacco containing tobacco accounted quality Ganoderma lucidum polysaccharides. 添加灵芝多糖的卷烟的制备方法:将灵芝多糖提取物加水配制成溶液,喷洒到烟丝上,自然晾干,再按常规卷烟工艺制成卷烟。 Preparation of GLP added cigarette: the Ganoderma lucidum polysaccharides extract solution was formulated by adding water, sprayed onto tobacco, air dry, then a conventional cigarette made from tobacco processes. 本发明与现有技术相比,在烟丝中含有灵芝多糖,在吸烟过程中可随主流烟气进入人体,通过血液循环被人体吸收,从而发挥其抗肿瘤、抗氧化、抗衰老的作用,卷烟烟气醇和无杂气,可提高卷烟质量,降低烟气对人体的危害性。 Compared with the prior art, Ganoderma lucidum polysaccharides contained in tobacco, the mainstream smoke enters the body may vary during smoking, is absorbed by the body through the blood circulation, and thus exert its anti-tumor, anti-oxidation, anti-aging effect, cigarette no alcohol, smoke odor, can improve the quality of cigarettes, to reduce smoke harm to the human body.

Description

灵芝多糖及其提取方法、添加灵芝多糖的卷烟及其制备方 Polysaccharides and their method of extraction of Ganoderma lucidum, Ganoderma lucidum polysaccharides added cigarette preparation thereof

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技术领域 FIELD

[0001] 本发明涉及一种添加到卷烟中的降低卷烟危害的提取物及其提取方法、添加该提取物的卷烟及其制备方法,特别是一种灵芝多糖及其提取方法、添加灵芝多糖的卷烟及其制备方法。 [0001] The present invention relates to an additive to reduce the harm of cigarette tobacco extract and the extraction method and preparation method of the cigarettes to add extract, ganoderma lucidum polysaccharides, and in particular a method for extraction, add polysaccharides from cigarette their preparation.

背景技术 Background technique

[0002] 据世界卫生组织的一项研究报告,全世界每年至少有100万人由于吸烟而过早地死亡。 [0002] According to a research report by the World Health Organization, at least 100 million people worldwide die prematurely due to smoking every year. 我国非常重视卷烟的降害工作,2005年以来,国家烟草专卖局组织开展了卷烟危害性指标体系和卷烟增香保润两项重大专项研究工作,取得了一定的成果。 Our country attaches great importance to the work of harm reduction cigarettes, since 2005, the State Tobacco Monopoly Bureau has embarked on a cigarette and cigarette hazard index system flavor-Run two major research projects, and achieved certain results. 如中国专利申请号200710052295. 7公开的一种增香保润的天然烟用添加剂,主要由罗汉果提取制备而成, 提升烟气,降低烟气干燥感。 The Chinese Patent Application No. 200710052295.7 discloses a flavoring-Run natural tobacco additives, mainly prepared from Luo Han Guo extract, to enhance the flue gas, the flue gas to reduce dry feeling. 中国专利申请号200610128283. 3公开的羊栖菜水提取液及其在烟草中的应用,将羊栖菜水提取液作为烟用料液加入到卷烟烟丝上,在燃吸卷烟时可产生特有的化学成分,明显改善或提高卷烟的质量品质,具有显著的生津作用和一定的利痰止咳作用,且能够减少卷烟主流烟气中的某些有害成分,不改变卷烟的原有性能,不改变或影响卷烟的吸食品质。 Chinese Patent Application No. 200610128283.3 disclosed Sargassum aqueous extract of tobacco and its application, as will Sargassum aqueous extract of tobacco material was added to the cut tobacco, the fuel may be generated when specific smoking cigarettes chemical composition, significantly improve the quality, or improving the quality of cigarettes, has a significant effect of fluid and certain benefits cough phlegm, and can reduce some of the harmful components in mainstream cigarette smoke, without changing the properties of the cigarette, without changing or quality of smoking cigarettes. 尽管如此,卷烟的增香保润还不能满足消费者多方面的需求。 Nevertheless, cigarette flavoring-Run can not meet the needs of many consumers.

发明内容 SUMMARY

[0003] 本发明的目的是提供一种灵芝多糖及其提取方法、添加灵芝多糖的卷烟及其制备方法,要解决的技术问题是提高烟气,降低卷烟中的有害成分。 [0003] The object of the present invention is to provide a method of extraction of Ganoderma lucidum polysaccharides and add polysaccharides from cigarette and its preparation method, the technical problem to be solved is to improve the smoke, to reduce the harmful components of cigarette.

[0004] 本发明采用以下技术方案:一种灵芝多糖,所述灵芝多糖用下述方法得到:一、 将灵芝菌体在30-70°C的温度下烘干,粉碎至10-100目;二、将粉碎后的灵芝菌体粉与80-100°C热水,按质量比1 : 10,在热水中浸提3次,每次5小时,合并3次的水浸提液;三、 过滤,滤液在0. 05Mpa,60°C,时间2小时条件下减压浓缩;四、加入浓缩后的滤液体积5-10 倍的50-98%乙醇,IO0C以下静置12小时以上,3000转/分钟,30分钟离心处理后,过滤得到灵芝多糖提取物。 [0004] The present invention employs the following technical solutions: A Ganoderma lucidum polysaccharides, the polysaccharide Ganoderma obtained by the following method: First, the cells Ganoderma dried at 30-70 ° C, and crushed to 10-100 mesh; Second, the powder was pulverized Ganoderma cells and 80-100 ° C hot water, a mass ratio of 1:10, extraction in hot water three times, each time 5 hours 3 times combined water extract; third the filtrate was concentrated under reduced pressure 0. 05Mpa, 60 ° C, 2 hours conditions; Fourth, the filtrate volume after addition of concentrated 5-10 fold 50 to 98% ethanol, IO0C allowed to stand for 12 hours or less, 3000 rev / min, after centrifugation for 30 minutes, filtered to obtain an extract of Ganoderma lucidum polysaccharides.

[0005] 一种灵芝多糖的提取方法,包括以下步骤:一、将灵芝菌体在30-70°C的温度下烘干,粉碎至10-100目;二、将粉碎后的灵芝菌体粉与80-100°C热水,按质量比1 : 10,在热水中浸提3次,每次5小时,合并3次的水浸提液;三、过滤,滤液在0. 05Mpa,60°C,时间2 小时条件下减压浓缩;四、加入浓缩后的滤液体积5-10倍的50-98%乙醇,10°C以下静置12 小时以上,3000转/分钟,30分钟离心处理后,过滤得到灵芝多糖提取物。 [0005] A method for extraction of Ganoderma lucidum polysaccharides, comprising the steps of: a, ganoderma cells at a temperature of 30-70 ° C drying, crushed to 10-100 mesh; two, the cells were pulverized powder Ganoderma and 80-100 ° C hot water, a mass ratio of 1:10, leached in hot water 3 times for 5 hours, the combined water extract 3 times; three, filtered and the filtrate 0. 05Mpa, 60 four 30 minutes centrifugation, the filtrate volume after addition of concentrated 5-10 fold 50 to 98% ethanol, 10 ° C was allowed to stand for 12 hours or less, 3000 rev / min,; ° C, 2 hours and concentrated under reduced pressure after filtration to obtain an extract of Ganoderma lucidum polysaccharides.

[0006] 一种添加灵芝多糖的卷烟,具有烟丝,所述烟丝中含有占烟丝质量0. 001-10 %的灵芝多糖;所述灵芝多糖用下述方法得到:一、将灵芝菌体在30-70°C的温度下烘干,粉碎至10-100目;二、将粉碎后的灵芝菌体粉与80-100°C热水,按质量比1 : 10,在热水中浸提3次,每次5小时,合并3次的水浸提液;三、过滤,滤液在0. 05Mpa, 60°C,时间2小时条件下减压浓缩;四、加入浓缩后的滤液体积5-10倍的50-98%乙醇,10°C以下静置12小时以上,3000转/分钟,30分钟离心处理后,过滤得到灵芝多糖提取物。 [0006] A cigarette added Ganoderma lucidum polysaccharides, with tobacco, the tobacco contained in the tobacco mass accounted for 0.5% of Ganoderma lucidum polysaccharides 001-10; Ganoderma lucidum polysaccharides obtained by the following method: First, 30 cells ganoderma drying at a temperature of -70 ° C., ground to 10-100 mesh; two, Ganoderma lucidum cells pulverized powder with 80-100 ° C hot water, a mass ratio of 1:10, extraction in hot water 3 times for 5 hours, the combined water extract 3 times; three, filtered and the filtrate was concentrated under reduced pressure 0. 05Mpa, 60 ° C, 2 hours conditions; filtrate volume four, was added concentrated 5-10 times of 50 to 98% ethanol, 10 ° C was allowed to stand for 12 hours or less, 3000 rev / min, after centrifugation for 30 minutes, filtered to obtain an extract of Ganoderma lucidum polysaccharides.

[0007] 本发明的烟丝中含有占烟丝质量0. 的灵芝多糖。 Tobacco [0007] the present invention contains 0. accounted tobacco quality Ganoderma lucidum polysaccharides.

[0008] 本发明的烟丝中含有占烟丝质量0. 01 %的灵芝多糖。 [0008] The shredded tobacco according to the present invention, 0.01% of Ganoderma lucidum polysaccharides containing tobacco mass accounted for.

[0009] 一种添加灵芝多糖的卷烟及其制备方法,包括以下步骤:将灵芝多糖提取物加水配制成质量浓度为1-10 %的溶液,按灵芝多糖占烟丝质量0. 001-10 %的比例喷洒到烟丝上,自然晾干,再按常规卷烟工艺制成卷烟;所述灵芝多糖的提取用以下步骤:一、将灵芝菌体在30-70°C的温度下烘干,粉碎至10-100目;二、将粉碎后的灵芝菌体粉与80-100°C 热水,按质量比1 : 10,在热水中浸提3次,每次5小时,合并3次的水浸提液;三、过滤, 滤液在0. 05Mpa,60°C,时间2小时条件下减压浓缩;四、加入浓缩后的滤液体积5-10倍的50-98%乙醇,IO0C以下静置12小时以上,3000转/分钟,30分钟离心处理后,过滤得到灵芝多糖提取物。 [0009] A cigarette add polysaccharides from their preparation method, comprising the steps of: adding water extract of Ganoderma lucidum polysaccharides were formulated to a concentration of 1-10 mass% solution, by Ganoderma polysaccharide is 0. 001-10% by mass of tobacco sprayed onto the tobacco ratio, air dry, then a conventional cigarette made from tobacco process; extracting polysaccharides from the following steps: First, the cells Ganoderma dried at 30-70 ° C, and pulverized to 10 -100 mesh; two, Ganoderma lucidum cells pulverized powder with 80-100 ° C hot water, a mass ratio of 1:10, extraction in hot water 3 times for 5 hours, the combined three times of flooding extract; three, filtered and the filtrate was concentrated under reduced pressure 0. 05Mpa, 60 ° C, 2 hours conditions; Fourth, the filtrate volume after addition of concentrated 5-10 fold 50 to 98% ethanol, IO0C the left 12 hour or more, 3000 rev / min, after centrifugation for 30 minutes, filtered to obtain an extract of Ganoderma lucidum polysaccharides.

[0010] 本发明与现有技术相比,在烟丝中含有灵芝多糖,灵芝多糖的溶解性好,在吸烟过程中可随主流烟气进入人体,通过血液循环被人体吸收,从而发挥其抗肿瘤、抗氧化、抗衰老的作用,卷烟烟气醇和无杂气,可提高卷烟质量,降低烟气对人体的危害性。 [0010] Compared with the prior art, in cut filler comprising Ganoderma lucidum polysaccharides, polysaccharides from good solubility, may enter the body with the mainstream smoke during smoking, it is absorbed by the body through the blood circulation, and thus exert its antineoplastic , anti-oxidation, anti-aging effect, alcohol, cigarette smoke-free mixed gas, can improve the quality of cigarettes, to reduce smoke harm to the human body.

具体实施方式 detailed description

[0011] 下面结合实施例对本发明作进一步详细说明。 [0011] The following embodiments in conjunction with embodiments of the present invention is described in further detail. 本发明的灵芝多糖的提取方法,采用以下步骤:一、将灵芝菌体去除杂质,在30-70°C的温度下烘干,粉碎至10-100目;二、将粉碎后的灵芝菌体粉与80-100°C热水,按质量比1 : 10,在热水中浸提3次,每次5小时, 合并3次的水浸提液;三、过滤,滤液在0. 05Mpa,60°C,时间2小时条件下减压浓缩;四、加入浓缩后的滤液体积5-10倍的50-98%乙醇,10°C以下静置12小时以上,3000转/分钟, 30分钟离心处理后,过滤得到灵芝多糖提取物。 Extraction of GLP to the present invention, the following steps: First, the cells Ganoderma remove impurities, dried at a temperature of 30-70 ° C, and crushed to 10-100 mesh; two, the cells were pulverized Ganoderma hot powder and 80-100 ° C, mass ratio of 1: 10, leached in hot water 3 times for 5 hours, the combined water extract 3 times; three, filtered and the filtrate 0. 05Mpa, centrifuged for 30 minutes four, the filtrate volume after addition of concentrated 5-10 fold 50 to 98% ethanol, 10 ° C was allowed to stand for 12 hours or less, 3000 rev / min,; 60 ° C, 2 hours and concentrated under reduced pressure after the treatment, and filtered to give an extract of Ganoderma lucidum polysaccharides.

[0012] 本发明的添加灵芝多糖的卷烟,烟丝中含有占烟丝质量0.001-10%的灵芝多糖。 [0012] Ganoderma lucidum polysaccharides of the present invention is added to cigarettes, tobacco containing tobacco accounted for 0.001 to 10% by mass of Ganoderma lucidum polysaccharides. 将所述灵芝多糖提取物加水配制成质量浓度为1-10%的溶液,按灵芝多糖占烟丝质量0. 001-10%的比例均勻喷洒到烟丝上,自然晾干,再按常规卷烟工艺制成卷烟。 The addition of water extract of Ganoderma lucidum polysaccharides formulated to a concentration of 1-10 mass% solution, by Ganoderma polysaccharide is tobacco mass ratio of 0. 001-10% by uniformly sprayed onto tobacco, air dry, then a conventional cigarette manufactured by the process into a cigarette.

[0013] 实施例1,取山东省泰安产的灵芝菌体,除去杂质,在60°C的温度下烘干,并粉碎至10-100目,用10倍质量的100°C热水,浸提3次,时间为每次5小时,合并3次的水浸提液,过滤,滤液在-0. 05Mpa、60°C,时间2小时条件下减压浓缩,将浓缩后的滤液加5倍体积的95%乙醇、10°C,静置12小时,3000转/分钟,30分钟离心处理,过滤,得到灵芝多糖。 [0013] Example 1, taken Tai Shandong Ganoderma producing cells, remove impurities, dried at a temperature of 60 ° C, and crushed to 10-100 mesh, 100 ° C 10 times the mass with water, dip mention three times, each time 5 hours, combined water extract 3 times, filtered, and the filtrate was concentrated under reduced pressure -0. 05Mpa, 60 ° C, 2 hours conditions, the filtrate was concentrated 5-fold increase volumes of 95% ethanol, 10 ° C, allowed to stand for 12 hours and 3000 rev / min, 30 min centrifugation, filtration, to obtain Ganoderma lucidum polysaccharides. 加水稀释后配制质量浓度为10%的溶液,按烟丝质量的0. 均勻喷洒到烟丝上,自然晾干, 按常规卷烟工艺制成卷烟。 Formulated diluted with water concentration of 10% solution, by the quality of the tobacco 0. uniformly sprayed onto tobacco, natural drying, a cigarette made by conventional cigarette processes.

[0014] 实施例2,取山东省泰安产的灵芝菌体,除去杂质,在50°C的温度下烘干,并粉碎至10-100目,用10倍质量的100°C热水,浸提3次,时间为每次5小时,合并3次的水浸提液,过滤,滤液在0. 05Mpa、60°C,时间2小时条件下减压浓缩,将浓缩后的滤液加10倍体积的75%乙醇、10°C,静置12小时,3000转/分钟,30分钟离心处理,过滤,得到灵芝多糖。 [0014] Example 2, taken Tai Shandong Ganoderma producing cells, remove impurities, dried at a temperature of 50 ° C, and crushed to 10-100 mesh, 100 ° C 10 times the mass with water, dip mention three times, each time 5 hours, combined water extract 3 times, filtered, and the filtrate was concentrated under reduced pressure 0. 05Mpa, 60 ° C, 2 hours conditions, the concentrated filtrate was added 10 volumes 75% ethanol, 10 ° C, allowed to stand for 12 hours and 3000 rev / min, centrifuged for 30 minutes, filtered, Ganoderma lucidum polysaccharides obtained. 加水稀释后配制质量浓度为8%的溶液,按烟丝质量的0.01%均勻喷洒到烟丝上,自然晾干, 按常规卷烟工艺制成卷烟。 Diluted with water formulated mass concentration of 8% solution, 0.01% by mass of tobacco uniformly sprayed onto tobacco, natural drying, a cigarette made by conventional cigarette processes.

[0015] 实施例3,取山东省泰安产的灵芝菌体,除去杂质,在30°C的温度下烘干,并粉碎至10-100目,用10倍质量的80°C热水,浸提3次,时间为每次5小时,合并3次的水浸提液,过滤,滤液在0. 05Mpa、60°C,时间2小时条件下减压浓缩,将浓缩后的滤液加10倍体积的50%乙醇、10°C,静置12小时,3000转/分钟,30分钟转速离心处理,过滤,得到灵芝多糖。 80 ° C water [0015] Example 3, taken Tai Shandong Ganoderma producing cells, remove impurities, dried at a temperature of 30 ° C, and crushed to 10-100 mesh, with 10 times the mass, dip mention three times, each time 5 hours, combined water extract 3 times, filtered, and the filtrate was concentrated under reduced pressure 0. 05Mpa, 60 ° C, 2 hours conditions, the concentrated filtrate was added 10 volumes 50% ethanol, 10 ° C, allowed to stand for 12 hours at 3000 rpm / min for 30 minutes and centrifuged, filtered, Ganoderma lucidum polysaccharides obtained. 加水稀释后配制质量浓度为8%的溶液,按烟丝质量的0.01%均勻喷洒到烟丝上,自然晾干,按常规卷烟工艺制成卷烟。 Diluted with water formulated mass concentration of 8% solution, 0.01% by mass of tobacco uniformly sprayed onto tobacco, natural drying, a cigarette made by conventional cigarette processes.

[0016] 实施例4,取山东省泰安产的灵芝菌体,除去杂质,在70°C的温度下烘干,并粉碎至10-100目,用10倍质量的80°C热水,浸提3次,时间为每次5小时,合并3次的水浸提液,过滤,滤液在0. 05Mpa、60°C,时间2小时条件下减压浓缩,将浓缩后的滤液加10倍体积的98%乙醇、10°C以下,静置12小时,3000转/分钟,30分钟转速,时间离心处理,过滤,得到灵芝多糖。 80 ° C water [0016] Example 4, taken Tai Shandong Ganoderma producing cells, remove impurities, dried at a temperature of 70 ° C, and crushed to 10-100 mesh, with 10 times the mass, dip mention three times, each time 5 hours, combined water extract 3 times, filtered, and the filtrate was concentrated under reduced pressure 0. 05Mpa, 60 ° C, 2 hours conditions, the concentrated filtrate was added 10 volumes 98% ethanol, 10 ° C or less, allowed to stand for 12 hours to 3000 rev / min, 30 rpm, time of centrifugation, filtration, to obtain Ganoderma lucidum polysaccharides. 加水稀释后配制质量浓度为8%的溶液,按烟丝质量的0. 01%均勻喷洒到烟丝上,自然晾干,按常规卷烟工艺制成卷烟。 Diluted with water formulated mass concentration of 8% solution, 0.01% by mass of tobacco uniformly sprayed onto tobacco, natural drying, a cigarette made by conventional cigarette processes.

[0017] 按照YC/T184-2004烟草及烟草制品,对烟草特有N-亚硝胺进行测定,按YC/ T30-1996卷烟烟气气相中一氧化碳的测定,本发明的添加灵芝多糖制作的卷烟与空白对照烟相比,降低烟气中的烟草特有亚硝胺20%、一氧化碳15%,无异味、杂气,有生津回甜、润喉、祛痰的作用。 [0017] according to YC / T184-2004 tobacco and tobacco products, tobacco-specific nitrosamines N- measurement, measured by YC / T30-1996 cigarette smoke of carbon monoxide in the gas phase, the present invention is added Ganoderma lucidum polysaccharides produced cigarettes and compared to the control cigarette smoke reducing tobacco specific nitrosamines in 20%, 15% carbon monoxide, no smell, odor, sweet animate Tianjin back effect, throat, expectorant. 采用GB15193. 1-1994《保健食品安全性毒理学评价规范》的方法动物毒性试验可降低呼吸道损伤。 Using GB15193. 1-1994 "health food safety toxicology evaluation norms" of animal toxicity testing methods can reduce the damage to the respiratory tract.

[0018] 本发明将灵芝多糖加入到烟丝中的量没有特别的限制,主要视吸烟时的口感来决定,其加入的质量可为0.001%,最高可占烟丝的10%。 [0018] The present invention will be added to the amount of tobacco shreds Ganoderma lucidum polysaccharides is not particularly limited, and depends mainly on the taste when smoking is determined, the added mass may be 0.001%, up to 10% of the tobacco.

[0019]灵芝多糖(Ganoderma lucidum Polysaccharide,GLP),现有技术已分离到的有200多种,其中大部分为β-葡聚糖,少数为α-葡聚糖,多糖链由三股单糖链构成,是一种螺旋状立体构形物,其立体构形和DNA、RNA相似,分子量从数百到数十万,大多存在于灵芝细胞内壁。 [0019] Ganoderma lucidum polysaccharides (Ganoderma lucidum Polysaccharide, GLP), the prior art has been isolated from more than 200, most of β- glucan, a small number of α- glucan polysaccharide chain which consists of three monosaccharide chain configuration, is a three-dimensional helical configuration thereof, a three-dimensional configuration and DNA, RNA similar molecular weight from several hundreds to several thousands, most of the cells present in the inner wall of Ganoderma lucidum. 灵芝多糖大多为异多糖,即除含有葡萄糖外,大多还含有少量阿拉伯糖、木糖、岩藻糖、鼠李糖、半乳糖等其他单糖。 Ganoderma lucidum polysaccharides mostly heteropolysaccharide, i.e., in addition to containing glucose, but most of them still contain a small amount of arabinose, xylose, fucose, rhamnose, galactose and other monosaccharides.

[0020] 灵芝多糖的药理作用:1、抗肿瘤作用,灵芝多糖对Lewis肺癌和结肠癌具有相当的抑制生长活性,并能增强正常小鼠腹腔巨噬细胞吞噬作用和荷瘤小鼠自然杀伤细胞(NK 细胞)活性,从而提高机体免疫功能。 [0020] The pharmacological effects of GLP: 1, anti-tumor effect, Ganoderma lucidum polysaccharides having Lewis lung and colon cancer growth inhibition activity comparable, and can enhance the normal mouse peritoneal macrophage phagocytosis and natural killer cells, tumor-bearing mice (NK cell) activity, thereby enhancing the immune function. 2、对免疫的调节作用,灵芝多糖能直接刺激人脐血单个核细胞CBMC增殖,并能协同植物凝血素PHA刺激CBMC增殖。 2, the regulation of the immune, Ganoderma lucidum polysaccharides can directly stimulate the proliferation of CBMC cord blood mononuclear cells, and can cooperate phytohemagglutinin PHA-stimulated proliferation of CBMC. 灵芝多糖可显著增加羊红细胞SRBC诱导的正常小鼠脾细胞中抗体形成细胞PFC的数目。 Ganoderma lucidum polysaccharides can significantly increase the number of antibody forming cells PFC sheep red blood cells SRBC spleen induced in normal mice. 3、抗氧自由基作用,灵芝多糖对OH-自由基的产生和红细胞脂质过氧化均有抑制作用,并对-OH有清除作用,具有SOD样物的活性,是灵芝延缓衰老的重要因素。 3, the role of oxygen free radicals, OH- produced GLP on free radical and lipid peroxidation in red blood cells was inhibited, and -OH scavenging effect, SOD-like active substance is an important factor in the aging of Ganoderma lucidum . 4、对细胞内SOD的影响,灵芝多糖对小白鼠红细胞内SOD的活性有明显的增强作用(P < 0. 001)。 4, the effect on intracellular SOD, Ganoderma lucidum polysaccharides significant enhancement of the activity of the mouse erythrocyte SOD (P <0. 001). 5、活血化瘀作用,灵芝多糖可明显地延长小鼠凝血时间,降低高脂血症小鼠血清中甘油三酯TG含量,能明显延长大鼠体内血栓形成的时间,抑制血瘀大鼠体外血栓的形成并降低血瘀大鼠的血浆比粘度。 5, blood circulation action, Ganoderma lucidum polysaccharides can significantly prolong the clotting time, decrease serum triglyceride TG hyperlipidemia mice, can significantly extend the time in rats in vivo thrombus formation, inhibits blood stasis rat thrombosis and reduces rats with blood plasma viscosity. 6、抗衰老作用,灵芝多糖可明显增强老年小鼠脾细胞内DNA多聚酶α的活性,与老年对照组相比分别增加44.0% 和58. 4%。 6, anti-aging effects, Ganoderma lucidum polysaccharides can significantly enhance the activity of splenocytes DNA polymerase α in aged mice, increased 44.0% and 58.4%, respectively, compared with aged control.

Claims (1)

1. 一种添加灵芝多糖的卷烟,具有烟丝,其特征在于:所述烟丝中含有占烟丝质量0. 或0.01%的灵芝多糖;所述灵芝多糖用下述方法得到:一、将灵芝菌体在30-70°C 的温度下烘干,粉碎至10-100目;二、将粉碎后的灵芝菌体粉与80-100°C热水,按质量比1 : 10,在热水中浸提3次,每次5小时,合并3次的水浸提液;三、过滤,滤液在0.05Mpa, 60°C,时间2小时条件下减压浓缩;四、加入浓缩后的滤液体积5-10倍的50-98%乙醇, IO0C以下静置12小时以上,3000转/分钟,30分钟离心处理后,过滤得到灵芝多糖提取物; 所述添加灵芝多糖的卷烟采用以下制备方法得到,包括以下步骤:将灵芝多糖提取物加水配制成质量浓度为1-10%的溶液,按灵芝多糖占烟丝质量0. 或0.01%的比例喷洒到烟丝上,自然晾干,再按常规卷烟工艺制成卷烟。 An add polysaccharides from cigarettes having tobacco, characterized in that: said tobacco containing tobacco accounts for 0.05 or 0.01% by mass of Ganoderma lucidum polysaccharides; Ganoderma lucidum polysaccharides obtained by the following method: First, cells ganoderma at a temperature of 30-70 ° C drying, crushed to 10-100 mesh; two, Ganoderma lucidum cells pulverized powder with 80-100 ° C hot water, a mass ratio of 1:10, immersed in hot water mention 3 times for 5 hours, the combined water extract 3 times; three, filtered and the filtrate was concentrated under reduced pressure 0.05Mpa, 60 ° C, 2 hours conditions; Fourth, the filtrate volume after addition of concentrated 5- 10 times 50 to 98% ethanol, IO0C allowed to stand for 12 hours or less, 3000 rev / min, after centrifugation for 30 minutes, filtered to obtain an extract of Ganoderma lucidum polysaccharides; the addition of GLP cigarette obtained by the following preparation method, comprising steps of: adding water extract of Ganoderma lucidum polysaccharides formulated to a concentration of 1-10 mass% solution, by Ganoderma polysaccharide is tobacco mass ratio of 0.05 or 0.01% was sprayed onto the shredded tobacco, natural drying, conventional press technology made cigarette cigarettes .
CN 200910189846 2009-09-01 2009-09-01 Polysaccharides and their method of extraction of Ganoderma lucidum, Ganoderma lucidum polysaccharides added cigarette and preparation method CN101693745B (en)

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CN102068034A (en) * 2010-12-30 2011-05-25 贵州中烟工业有限责任公司 Method for reducing tar and harm of papermaking reconstituted tobacco
CN102093486B (en) * 2011-01-04 2012-09-05 广东省微生物研究所 Decolorized ganoderma lucidum polysaccharide extract and method for preparing hand cream by using same
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CN102295709B (en) * 2011-07-02 2013-04-10 上海市农业科学院 Ganoderma lucidum polysaccharide with light color and high molecular weight, and preparation method thereof
CN102697170B (en) * 2012-07-02 2015-01-07 广西中烟工业有限责任公司 Aroma-increasing and humidity-preserving additive for cigarettes and preparation method and application thereof
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CN102793263B (en) * 2012-08-27 2016-01-20 福建中烟工业有限责任公司 The extracting method of white fungus water extracting liquid and the application in tobacco thereof
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CN104126867B (en) * 2014-07-18 2015-10-21 云南中烟工业有限责任公司 The extracting method of speciosus polysaccharide and the purposes in tobacco product thereof
CN104193840B (en) * 2014-07-31 2016-08-31 上海市农业科学院 A kind of lucid ganoderma spore powder polysaccharide and preparation method thereof
CN104387495A (en) * 2014-12-14 2015-03-04 桂林宏旺菌业股份有限公司 Preparation method of ganoderma lucidum polysaccharide

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