CN101928353B - Extraction of cordyceps polysaccharide and application of cordyceps polysaccharide in cigarettes - Google Patents
Extraction of cordyceps polysaccharide and application of cordyceps polysaccharide in cigarettes Download PDFInfo
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- CN101928353B CN101928353B CN2010102587125A CN201010258712A CN101928353B CN 101928353 B CN101928353 B CN 101928353B CN 2010102587125 A CN2010102587125 A CN 2010102587125A CN 201010258712 A CN201010258712 A CN 201010258712A CN 101928353 B CN101928353 B CN 101928353B
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Abstract
The invention discloses extraction of cordyceps polysaccharide and application of the cordyceps polysaccharide to cigarettes and relates to a process for extracting the cordyceps polysaccharide from cordyceps sinensis and an application method thereof in the cigarettes. The extraction of the cordyceps polysaccharide comprises the following steps of: after preprocessing the cordyceps sinensis, grinding the preprocessed cordyceps sinensis; adding water or ethanol into the ground cordyceps sinensis for extraction; after filtering and concentrating, adding the ethanol for standing; and then filtering and drying to obtain the cordyceps polysaccharide. The prepared cordyceps polysaccharide is dissolved in the water and then is uniformly mixed with tobacco shred. Tests show that compared with the contrasted cigarette, the cigarette rolled by the invention has the certain objectionable constituent content of the mainstream smoke, such as smoke benzopyrene, tobacco-specific nitrosamine, gas phase free radicals in smoke and the like reduced by 10 to 35 percent, and particularly has better effect of reducing free radicals in the smoke of the cigarette; when the cigarette is burnt and smoked, the smoke does not have peculiar smell; and the cigarette has the effects of promoting the production of body fluid, smoothing throat, reducing phlegm and the like.
Description
Technical field
A kind of extraction of Cordyceps Polysaccharide and the application in cigarette thereof relate to and from entomophyte, extract Cordyceps Polysaccharide and the application method in cigarette thereof.
Background technology
Along with smoking and healthy problem more and more receive publicity, the objectionable constituent of sucking security, increase nourishing function, reducing in the cigarette smoke that improve cigarette are the inevitable development trend of cigarette.
Entomophyte is one type of crucial medicinal fungi, is to parasitize the entomogenous fungi complex body that fungi and its host insect on the insect body form, and is under the jurisdiction of Ascomycetes (Ascomycetes), Clavicipitaceae (Claricipitaceae), Cordyceps (Cordyceps).Research in recent years shows: entomophyte have calmness, antibacterial, suppress many-sided pharmacological actions such as mouse ascites cancer, enhancing body immunologic function.Entomophyte has various active composition (for example polysaccharide), and these activeconstituentss have pharmacological action widely.A large amount of medical experiments confirm, but the Cordyceps Polysaccharide activated macrophage stimulates antibody to produce, and improves body immunity.Cordyceps polysaccharide (CP) is hindered chmice acute chemical liver and is had provide protection, and the experiment of Kiho etc. shows that Cordyceps polysaccharide has the effect of remarkable function of reducing blood sugar bioactivity, the effective constituent of aweto nourishing and nourishing yin effect to diabetic mice.
Summary of the invention
The objective of the invention is the Cordyceps Polysaccharide extraction separation is come out, and be applied in the cigarette,, and reduce some objectionable constituent in the cigarette mainstream flue gas with the organoleptic quality of raising cigarette.
Cordyceps Polysaccharide of the present invention is to be made by following method:
(1) entomophyte is being crushed to the 40-100 order after the oven dry down at 30-60 ℃, or under the liquid nitrogen condition, is being crushed to the 40-100 order after washing, removing the processing in early stage of impurity, airing;
(2) under 80-100 ℃ or UW or microwave condition, water or ethanol carried out reflux 1-10 hour pulverizing back entomophyte;
(3) filtering and concentrating, the concentration that makes filtering and concentrating liquid contain dry-matter is 1-10%;
(4) in concentrated filtrate, adding purity is 90%-98% ethanol, and concentrate feed liquid: the alcoholic acid volume ratio is 1: 3, is leaving standstill below 10 ℃ more than 8 hours behind the mixing, after filtering, drying, promptly obtains Cordyceps Polysaccharide of the present invention.
With Cordyceps Polysaccharide of the present invention through adding on the cigarette shreds with feed liquid as cigarette after the water dissolution; Concrete usage ratio is: the consumption of Cordyceps Polysaccharide solution is the 0.001-0.2% of pipe tobacco weight; Cordyceps Polysaccharide is a pale solid; Water soluble; Cordyceps Polysaccharide strength of solution after water dissolution is 1-10% when using, and adds Cordyceps Polysaccharide on the pipe tobacco to according to weight percent 0.1-2% through the spray add mode.
Substantive features of the present invention are peculiar chemical ingredientss of utilizing Cordyceps Polysaccharide when cigarette burns and sucks, to produce, example: 3-methyl-2,5-furandione; 2-Pyrrolidone, 1,6-dehydration-β-D-glucose; 1, compositions such as 4-dehydration-D-N.F,USP MANNITOL, these compositions can obviously improve or improve the quality quality of cigarette; Have the effect of promoting the production of body fluid significantly and certain relieving cough function, can reduce the content of some objectionable constituent in the cigarette mainstream flue gas, and not change original performance of cigarette; Improve the sucking quality of cigarette, therefore have the meaning and the using value of important actual production.
The present invention compares prior art and has the following advantages:
1. can improve the sucking quality of cigarette;
2. can reduce some objectionable constituent in the cigarette mainstream flue gas;
3. do not change the original performance of cigarette and the complete processing of cigarette;
4. has certain humectation effect;
5. has effects such as promoting the production of body fluid, wet one's whistle, eliminate the phlegm, reduce stimulation;
6. without any side effects.
Through testing: the cigarette by the present invention rolled is compared with the contrast cigarette; The content of some objectionable constituent in the cigarette mainstream flue gas; Like flue gas benzopyrene, tobacco-specific nitrosamine, smoke gaseous phase free radical etc.; The reduction amplitude is between 10-35%, and especially effect is better aspect reduction cigarette smoke radical; When cigarette burns and sucks, the flue gas free from extraneous odour, have promote the production of body fluid, wet one's whistle, effect such as sharp phlegm.
Embodiment
Embodiment 1:
Get the entomophyte dry product, the water washes clean, pick out impurity after, dry or dry.Dry the back down or under liquid nitrogen, be crushed to the 40-100 order at 50 ℃; Under 90 ℃ of conditions, the extraction using alcohol entomophyte of water or 20-80% concentration 7 hours is after the content that filtrate filtered is concentrated to dry-matter is 1%-10%; With the filtrating after concentrating; By filtrating: ethanol is that 1: 3 volume ratio adds in the filtrating after concentrating, and under 4 ℃, leaves standstill more than the 8h behind the mixing, after centrifugal, filtration, drying; Promptly get Cordyceps Polysaccharide of the present invention, be pale solid;
Cordyceps Polysaccharide joins on the cigarette shreds with feed liquid through processing cigarette after the water dissolution, and concrete applying method is: the consumption of Cordyceps Polysaccharide is 0.002% of a pipe tobacco weight, is added on the pipe tobacco through the spray add mode behind the dilute with water; The concentration of Cordyceps Polysaccharide solution is 5% (weight ratio), stored refrigerated.
Embodiment 2:
Get the entomophyte dry product, be crushed to 40-100 order after handling premenstruum; Under UW or microwave condition, with water extraction entomophyte 2 hours, after the content that filtrate filtered is concentrated to dry-matter is 10%; With the filtrating after concentrating; By filtrating: ethanol is that 1: 3 volume ratio adds ethanol (concentration 95%), under 4 ℃, leaves standstill more than the 8h behind the mixing, after centrifugal, filtration, drying; Promptly get Cordyceps Polysaccharide of the present invention, be pale solid;
Cordyceps Polysaccharide is added to method in the pipe tobacco with embodiment 1.
Embodiment 3:
Get the entomophyte dry product, after handling premenstruum, after oven dry under 40 ℃, be crushed to the 10-100 order; The water that adds 10~50 times of entomophyte weight, room temperature oscillation extraction entomophyte 4h is after the content that filtrate filtered is concentrated to dry-matter is 10%; With the filtrating after concentrating; By filtrating: ethanol is that 1: 3 volume ratio adds ethanol (concentration 95%), under 4 ℃, leave standstill more than the 8h behind the mixing, through centrifugal, filter after; Promptly get Cordyceps Polysaccharide of the present invention, be pale solid.
Cordyceps Polysaccharide is added to method in the pipe tobacco with embodiment 1.
Claims (3)
1. the process for extracting of a Cordyceps Polysaccharide is characterized in that, the process for extracting of Cordyceps Polysaccharide is:
A, entomophyte are being crushed to the 40-100 order after the oven dry down at 30-60 ℃, or under the liquid nitrogen condition, are being crushed to the 40-100 order after washing, removing the processing in early stage of impurity, airing;
B, under 80-100 ℃ or UW or microwave condition, water or ethanol carried out reflux 1-10 hour pulverizing back entomophyte;
The c filtering and concentrating, the concentration that makes filtering and concentrating liquid contain dry-matter is 1-10%;
D, in concentrated filtrate, to add purity be 90%-98% ethanol, and concentrate feed liquid: the alcoholic acid volume ratio is 1: 3, is leaving standstill below 10 ℃ more than 8 hours behind the mixing, after filtering, drying, promptly obtains Cordyceps Polysaccharide.
2. method according to claim 1 is extracted the application of Cordyceps Polysaccharide in cigarette that obtains, and it is characterized in that Cordyceps Polysaccharide through adding on the cigarette shreds with feed liquid as cigarette after the water dissolution; Concrete usage ratio is: the consumption of Cordyceps Polysaccharide solution is the 0.001-0.2% of pipe tobacco weight; Cordyceps Polysaccharide is a pale solid; Water soluble; Cordyceps Polysaccharide strength of solution after water dissolution is 1-10% when using, and adds Cordyceps Polysaccharide on the pipe tobacco to according to weight percent 0.1-2% through the spray add mode.
3. the process for extracting of Cordyceps Polysaccharide according to claim 1, the technology that it is characterized in that the reflux of entomophyte can be used at room temperature, and the method for oscillation extraction substitutes.
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CN2010102587125A CN101928353B (en) | 2010-08-13 | 2010-08-13 | Extraction of cordyceps polysaccharide and application of cordyceps polysaccharide in cigarettes |
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CN2010102587125A CN101928353B (en) | 2010-08-13 | 2010-08-13 | Extraction of cordyceps polysaccharide and application of cordyceps polysaccharide in cigarettes |
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CN101928353B true CN101928353B (en) | 2012-07-18 |
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Families Citing this family (5)
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CN102367281B (en) * | 2011-10-20 | 2013-07-17 | 广东中烟工业有限责任公司 | Preparation method and application of ethanol extract of laminarin |
CN102796204A (en) * | 2012-08-27 | 2012-11-28 | 福建中烟工业有限责任公司 | Extraction method of tremella polysaccharide and application of tremella polysaccharide in tobaccos |
CN102793263B (en) * | 2012-08-27 | 2016-01-20 | 福建中烟工业有限责任公司 | The extracting method of white fungus water extracting liquid and the application in tobacco thereof |
CN105011350A (en) * | 2014-04-30 | 2015-11-04 | 上海绿馨电子科技有限公司 | Electronic cigarette oil containing cordyceps and preparation method thereof |
CN110283660A (en) * | 2019-06-28 | 2019-09-27 | 云南中烟工业有限责任公司 | A kind of preparation method and application of microwave-assisted tobacco humectant |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005113607A2 (en) * | 2004-05-21 | 2005-12-01 | Chiron Srl | Measuring degree of polymerisation for capsular saccharides that contain sialic acid |
CN101307112A (en) * | 2008-07-07 | 2008-11-19 | 中国科学院微生物研究所 | Process for abstracting mycelium polysaccharide by cordyceps fermentation |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2005113607A2 (en) * | 2004-05-21 | 2005-12-01 | Chiron Srl | Measuring degree of polymerisation for capsular saccharides that contain sialic acid |
CN101307112A (en) * | 2008-07-07 | 2008-11-19 | 中国科学院微生物研究所 | Process for abstracting mycelium polysaccharide by cordyceps fermentation |
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