CN101658138A - Optimized substrate for tissue culture, transplantation and hardening-off of gerbera jamesonii - Google Patents
Optimized substrate for tissue culture, transplantation and hardening-off of gerbera jamesonii Download PDFInfo
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- CN101658138A CN101658138A CN200910112464A CN200910112464A CN101658138A CN 101658138 A CN101658138 A CN 101658138A CN 200910112464 A CN200910112464 A CN 200910112464A CN 200910112464 A CN200910112464 A CN 200910112464A CN 101658138 A CN101658138 A CN 101658138A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
An optimized substrate for tissue culture, transplantation and hardening-off of gerbera jamesonii relates to a method for tissue culture of flowers, which is characterized in that the substrate mainlyconsists of saw dust, moor soil and laterite according to the volume proportion of 2:1:1, and the pH value of the substrate is controlled between 6.2 and 6.8. The method has the following advantagesthat: the adding of the saw dust saves cost, and simultaneously improves the ventilation capability and water permeability of the substrate; as a conventional component of a soilless culture medium, the moor soil guarantees various physico-chemical structures of the substrate; and the adding of the laterite not only can adjust the pH value of the substrate, but also can enhance the water-holding and fertilizer-conserving capacity of the substrate.
Description
Technical field:
The present invention relates to flowers tissue cultivating seedling mating system, particularly the flameray gerbera tissue cultivating seedling is transplanted hardening matrix.
Background technology:
Flameray gerbera, formal name used at school Gerbera jamesonii Bolus is the perennial perennial root herbaceous plant of composite family Gerbera, is one of the world's five big cut-flowers, its flower is very large, and spray is tall and straight, and it is long that bottle is inserted the life-span, the meaning of " helping man, the cause prosperity " is arranged again, thereby enjoy consumer's favor.
Because the most selfing of flameray gerbera is infertile, filial generation can be divided and make a variation, and offshoot is adopted in conventional breeding, but reproduction coefficient is low, can not satisfy the needs of large-scale production.For obtaining seedling a large amount of high-qualitys, virus-free rapidly, utilizing tissue culture technique to carry out the production of flameray gerbera seedling is a kind of valid approach, and the transplanting of test-tube plantlet domestication is one step of key in fast numerous process, for guaranteeing that the test-tube plantlet plantation survives in a large number, should create the environmental condition that is fit to the seedling growth as much as possible.Wherein transplanting medium is to influence test-tube plantlet to tame one of key factor that survives, the whether suitable survival rate that often determines tissue cultivating seedling of transplanting medium, sturdy degree and growing way, have only and select suitable transplanting medium, create good environmental condition, just can cultivate the high-quality seedling.
A, tissue cultivating seedling are transplanted the composition and the ratio of hardening matrix:
Generally adopt components such as vermiculite, perlite, rice husk and the peat composed of rotten mosses about flameray gerbera group training seedling replanting matrix in the prior art, there are some researches show that flameray gerbera seedling survival rate and growing way on the matrix of 1/3 perlite+2/3 turfy soil are best; Also there is the part Study result to show that two kinds of 75% peanut shell matrix+25% perlites, 75% peanut shell matrix+25% slag cooperate the effect of matrix obviously to be better than the Cornell and do not have native mixed-matrix.
The control of B, matrix pH value;
The transplanting medium pH value of flameray gerbera seedling generally should be controlled between 6.2～6.8, can regulate the pH value of matrix by adding quicklime.
The shortcoming of prior art: one, matrix production cost height, part constituent are difficult to obtain; Two, the prescription of soilless substrate has guaranteed the venting and water permeable ability of matrix, but the water holding fertilizer-preserving ability is poor simultaneously, is unfavorable for seedling early growth.Three, by transplanting topdressing of later stage, increased labour cost.
Summary of the invention:
The objective of the invention is to overcome above-mentioned defective, a kind of optimization matrix of tissue culture, transplantation and hardening-off of gerbera jamesonii is provided.
The solution of the present invention is: matrix mainly is made up of sawdust, peat soil and red soil, and by volume ratio is 2: 1: 1, and the matrix pH value should be controlled between 6.2～6.8.
The invention has the advantages that: the adding of sawdust provides cost savings, and has increased the venting and water permeable ability of matrix simultaneously; Peat soil has guaranteed the various physics and chemistry structures of matrix as a kind of soilless culture substrate component of routine; The adding of red soil can be regulated the pH value of matrix, also can strengthen the preserve moisture and fertility ability of matrix simultaneously.
Embodiment:
Matrix of the present invention mainly is made up of sawdust, peat soil and red soil, and by volume ratio is 2: 1: 1, and the matrix pH value should be controlled between 6.2～6.8.
In matrix, use the fertilizer of 1/25～1/5 matrix volume and the calcium magnesium phosphorus of 1/150～1/50 matrix volume.
The interpolation of fertilizer just in order to reduce the management of topdressing in later stage, reduces labour cost, and the interpolation of fertilizer can be used as Rights attached thereto, fertilizer 1/5-1/25 volume, calcium magnesium phosphorus 1/50-1/150 volume.
PH value is regulated the pH value of matrix by adding quicklime.
A large amount of research and practices think that the fertilizer nutrient is comprehensive, and fertilizer efficiency is lasting.Not only contain the essential macroelement of plant, trace element in the fertilizer, also contain abundant organic nutrient, fertilizer is the most comprehensive fertilizer.Fertilizer can improve soil physical and chemical property, increases soil fertility, and can promote the activity of edaphon.Calcium is that plant is kept one of necessary nutritive element of normal activities, carries out different physiological roles in plant corpus; At present, calcium no longer only is counted as a kind of nutriment that participates in constituting the various organs of plant, tissue, but also regulates cell function as semiochemicals, is normally providing safeguard of various metabolic activities in the plant corpus; In addition, calcium also plays a significant role to the rely improvement of soil media of growth of plant.Magnesium is the constituent of plant chlorophyll, and it is a metallic element unique in the chlorophyll molecule; Magnesium is the activator of plurality of enzymes; Magnesium is the neccessary composition of polysome, can also promote to do the formation of vitamin A, C in the object.Therefore, use the photosynthesis that fertiliser containing magnesium can promote crops, promote the synthetic of protein, improve the output and the quality of improving agricultural product of crops.According to another report, magnesium can also promote the absorption of plant to silicon, helps preventing the germ intrusion; Can also reduce the absorption of plant, alleviate the disease effect of manganese when too much manganese; When executing the magnesium of low concentration, can also promote the absorption of calcium.Phosphorus is to form indispensable elements such as cell nucleus albumen, lecithin.P elements can quicken cell division, impels root system and overground part to accelerate growth, promotes flower bud differentiation.
This matrix can guarantee the needed various physicochemical properties of flameray gerbera seedling early growth, guarantees the fertility requirement in the tissue cultivating seedling process of growth simultaneously.Utilize this matrix to carry out the flameray gerbera tissue cultivating seedling and transplant hardening, planting shoot survival percent can reach more than 98%, and the seedling early growth stalwartness, meet batch production produce in to the requirement of seedling, and reduced production cost.
Advantage of the present invention and principle:
The optimization that flameray gerbera tissue cultivating seedling of the present invention is transplanted hardening matrix has tangible advantage:
One, matrix adopt sawdust, peat soil and the red soil ultimate constituent as matrix, and its ratio is 2: 1: 1, takes into account matrix gas permeability and water holding fertilizer-preserving ability, can guarantee that the physicochemical property of matrix helps the growth of seedling;
Its two, be that the cost of the transplanting hardening matrix of constituent is starkly lower than hardening matrix formulations commonly used in the present production with sawdust, peat soil and red soil;
Its three, add a certain amount of fertilizer and calcium magnesium phosphorus and can guarantee fertility requirement after seedling recovers growth.
Principle: in the seedling production process, the transplanting hardening process of tissue cultivating seedling is a crucial step, and the preparation of matrix is related to the transplanting survival rate and the growing way of seedling, and good aeration water penetration and water holding fertilizer-preserving ability are the key conditions that guarantees seedling early growth.The experimental study result shows that the matrix formulations of the technology of the present invention is at the matrix formulations that obviously is better than prior art aspect the water holding nutrient preserving capability energy, and the matrix formulations of the technology of the present invention has also fully guaranteed the venting and water permeable ability of matrix simultaneously.Under same Routine Management, adopt the kind shoot survival percent of the matrix formulations in the technology of the present invention higher by 12% than the kind shoot survival percent of the matrix formulations of prior art, significantly improved planting percent, reduced production cost.
Claims (2)
1, the optimization matrix of tissue culture, transplantation and hardening-off of gerbera jamesonii is characterized in that matrix mainly is made up of sawdust, peat soil and red soil, and by volume ratio is 2: 1: 1, and the matrix pH value should be controlled between 6.2～6.8.
2,, it is characterized in that in matrix, using the fertilizer of 1/25～1/5 matrix volume and the calcium magnesium phosphorus of 1/150～1/50 matrix volume according to the optimization matrix of right 1 described tissue culture, transplantation and hardening-off of gerbera jamesonii.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101124640A CN101658138B (en) | 2009-09-07 | 2009-09-07 | Optimized substrate for tissue culture, transplantation and hardening-off of gerbera jamesonii |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101124640A CN101658138B (en) | 2009-09-07 | 2009-09-07 | Optimized substrate for tissue culture, transplantation and hardening-off of gerbera jamesonii |
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| Publication Number | Publication Date |
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| CN101658138A true CN101658138A (en) | 2010-03-03 |
| CN101658138B CN101658138B (en) | 2012-01-04 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172151A (en) * | 2011-02-23 | 2011-09-07 | 广东省农业科学院果树研究所 | Transplanting matrix for tissue culture seedlings of pineapple |
| CN104737892A (en) * | 2015-04-17 | 2015-07-01 | 文山苗乡三七科技有限公司 | Pseudo-ginseng seedling substrate and preparation method thereof |
| CN105638404A (en) * | 2015-12-30 | 2016-06-08 | 江苏苏港和顺生物科技有限公司 | Soilless culture substrate special for flowers |
| CN106105669A (en) * | 2016-06-29 | 2016-11-16 | 无锡南理工科技发展有限公司 | A kind of African Chrysanthemum transplants substrate and the application thereof of seedling exercising |
| CN108401905A (en) * | 2018-04-10 | 2018-08-17 | 宜宾云辰乔木园林有限责任公司 | A kind of method of African Chrysanthemum rapid propagation cultivation and whole year production fresh flower |
| CN111248052A (en) * | 2020-02-21 | 2020-06-09 | 广东东升实业集团有限公司 | Method for preparing garden matrix by using sand washing sludge of open-air quarrying mine |
| CN112075299A (en) * | 2020-09-23 | 2020-12-15 | 广东省农业科学院果树研究所 | Banana hemp No. 1 transplanting method |
| CN114375800A (en) * | 2022-01-12 | 2022-04-22 | 云南省农业科学院花卉研究所 | African daisy culture medium and efficient culture method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000191421A (en) * | 1998-10-20 | 2000-07-11 | Osamu Matsudaira | Plant growth promoter |
| CN101213939A (en) * | 2008-01-14 | 2008-07-09 | 中国科学院华南植物园 | In-vitro high-frequency regeneration replication method of African chrysanthemum |
-
2009
- 2009-09-07 CN CN2009101124640A patent/CN101658138B/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172151A (en) * | 2011-02-23 | 2011-09-07 | 广东省农业科学院果树研究所 | Transplanting matrix for tissue culture seedlings of pineapple |
| CN104737892A (en) * | 2015-04-17 | 2015-07-01 | 文山苗乡三七科技有限公司 | Pseudo-ginseng seedling substrate and preparation method thereof |
| CN105638404A (en) * | 2015-12-30 | 2016-06-08 | 江苏苏港和顺生物科技有限公司 | Soilless culture substrate special for flowers |
| CN106105669A (en) * | 2016-06-29 | 2016-11-16 | 无锡南理工科技发展有限公司 | A kind of African Chrysanthemum transplants substrate and the application thereof of seedling exercising |
| CN108401905A (en) * | 2018-04-10 | 2018-08-17 | 宜宾云辰乔木园林有限责任公司 | A kind of method of African Chrysanthemum rapid propagation cultivation and whole year production fresh flower |
| CN108401905B (en) * | 2018-04-10 | 2021-07-13 | 四川云辰园林科技有限公司 | Method for rapid propagation culture and annual fresh flower production of gerbera jamesonii |
| CN111248052A (en) * | 2020-02-21 | 2020-06-09 | 广东东升实业集团有限公司 | Method for preparing garden matrix by using sand washing sludge of open-air quarrying mine |
| CN112075299A (en) * | 2020-09-23 | 2020-12-15 | 广东省农业科学院果树研究所 | Banana hemp No. 1 transplanting method |
| CN114375800A (en) * | 2022-01-12 | 2022-04-22 | 云南省农业科学院花卉研究所 | African daisy culture medium and efficient culture method |
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| CN101658138B (en) | 2012-01-04 |
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