CN102172151A - Transplanting matrix for tissue culture seedlings of pineapple - Google Patents
Transplanting matrix for tissue culture seedlings of pineapple Download PDFInfo
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- CN102172151A CN102172151A CN 201110042547 CN201110042547A CN102172151A CN 102172151 A CN102172151 A CN 102172151A CN 201110042547 CN201110042547 CN 201110042547 CN 201110042547 A CN201110042547 A CN 201110042547A CN 102172151 A CN102172151 A CN 102172151A
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Abstract
The invention discloses a transplanting matrix for tissue culture seedlings of pineapple. The transplanting matrix consists of an upper layer and a lower layer, wherein the upper layer is a peat soil layer with the thickness of 3 to 4 centimeters, the lower layer is a mixing layer which is formed by uniformly mixing yellow mud, chaff and peat soil in a mass ratio of (8-6) :(3-4):1, and the thickness of the mixing layer is not less than 8 centimeters. The transplanting matrix for the tissue culture seedlings of the pineapple has strong capability of water and nutrient preserving; the tissue culture seedlings of the pineapple transplanted on the transplanting matrix grow quickly; plants grow more quickly along with thickened and increased root systems particularly after being transplanted for 60 days and raise obviously with multiple leaves in long and wide shapes; the survival rate of the tissue culture seedlings is improved obviously; quick rooting is achieved; root coefficient and root length are obviously increased; the plants can basically come out of nursery after being transplanted for 150 days; and the time of the plants coming out of the nursery can be effectively shortened to facilitate the reduction of culture cost on the tissue culture seedlings of the pineapple.
Description
Technical field
The present invention relates to a kind of pineapple tissue cultivating seedling transplanting medium.
Background technology
Pineapple is one of tropical fruit (tree) of high-quality, and is big in China South China cultivation amount.
Usually pineapple adopts the bud seedling to carry out breeding and culturing, but the reproduction coefficient of bud seedling is lower, and speed is slow.Conventional variety generally only adopts the breeding of bud seedling can satisfy the demand of large-scale planting because the plantation amount is big.But when new varieties are promoted, adopt the breeding of bud seedling, far can not satisfy the needs of large-scale planting.In this case, generally obtain tissue cultivating seedling by tissue culture, new varieties are promoted and the needs of large-scale planting to satisfy.At present, pineapple tissue cultivating seedling transplanting medium is based on sandy (river sand) seedbed, its preserve moisture and fertility power is poor, the leaf of tissue cultivating seedling is long, plant height increasess slowly, root growth is few and short, and the survival rate of tissue cultivating seedling is also lower, generally about 80%, the production efficiency that this greatly reduces tissue cultivating seedling has increased the production cost of tissue cultivating seedling.
Summary of the invention
The object of the present invention is to provide a kind of pineapple tissue cultivating seedling transplanting medium.
The technical solution used in the present invention is:
A kind of pineapple tissue cultivating seedling transplanting medium, constitute by two-layer up and down, the upper strata is peat soil layer (thick 5～20 mm of grain), its thickness is 3～4 cm, lower floor is a mixed layer, mixed by the mass ratio of 8～6:3～4:1 by yellow mud, husk, peat soil (thick 5～40 mm of grain) and to form, its thickness is not less than 8 cm.
Preferably, the thickness of mixed layer is 8～9 cm.
Preferably, mixed layer is mixed by the mass ratio of 6:4:1 by yellow mud, husk, peat soil and forms.
Pineapple tissue cultivating seedling transplanting medium of the present invention, the preserve moisture and fertility ability is strong, transplants pineapple tissue cultivating seedling thereon, growth is fast, particularly after transplanting 60 d, along with root system increases slightly and increases, plant strain growth is faster, blade is many and long, plant increases obviously, and the survival rate of tissue cultivating seedling is significantly increased, and the root of hair rate improves, can effectively shorten seedling-growing time, help to reduce the cultivation cost of pineapple tissue cultivating seedling.
The thickness of mixed layer is 8～9 cm, is enough to guarantee the needs of tissue cultivating seedling root growth, avoids thickness excessive, increases the cost of matrix.
Description of drawings
Fig. 1 is the figure that influences that newly takes out the number of blade after different substrates is transplanted the pineapple tissue cultivating seedling;
Fig. 2 is different substrates is transplanted rear blade length to the pineapple tissue cultivating seedling the figure that influences;
Fig. 3 is different substrates is transplanted the rear blade width to the pineapple tissue cultivating seedling the figure that influences;
Fig. 4 is different substrates is transplanted back, back plant height to the pineapple tissue cultivating seedling the figure that influences.
Embodiment
A kind of pineapple tissue cultivating seedling transplanting medium, constitute by two-layer up and down, the upper strata is peat soil layer (thick 5～20 mm of grain), its thickness is 3～4 cm, lower floor is a mixed layer, mixed by the mass ratio of 8～6:3～4:1 by yellow mud, husk, peat soil (thick 5～40 mm of grain) and to form, its thickness is not less than 8 cm.
Preferably, the thickness of mixed layer is 8～9 cm.
Preferably, mixed layer is mixed by the mass ratio of 6:4:1 by yellow mud, husk, peat soil and forms.
Below in conjunction with embodiment, further specify the present invention.
Embodiment 1
Pineapple tissue cultivating seedling transplanting medium is made of two-layer up and down, and the upper strata is the peat soil layer, and its thickness is 3～4 cm, and lower floor is a mixed layer, is mixed by the mass ratio of 6:4:1 by yellow mud, husk, peat soil to form, and its thickness is 8～9 cm.
Pineapple tissue cultivating seedling transplanting medium is made of two-layer up and down, and the upper strata is the peat soil layer, and its thickness is 3～4 cm, and lower floor is a mixed layer, is mixed by the mass ratio of 8:4:1 by yellow mud, husk, peat soil to form, and its thickness is 8～9 cm.
Embodiment 3
Pineapple tissue cultivating seedling transplanting medium is made of two-layer up and down, and the upper strata is the peat soil layer, and its thickness is 3～4 cm, and lower floor is a mixed layer, is mixed by the mass ratio of 7:3:1 by yellow mud, husk, peat soil to form, and its thickness is 8～9 cm.
Embodiment 4
Pineapple tissue cultivating seedling transplanting medium is made of two-layer up and down, and the upper strata is the peat soil layer, and its thickness is 3～4 cm, and lower floor is a mixed layer, is mixed by the mass ratio of 8:3:1 by yellow mud, husk, peat soil to form, and its thickness is 8～9 cm.
The pineapple tissue cultivating seedling is transplanted test:
Pineapple tissue cultivating seedling transplanting medium
Test matrix: peat soil matrix (the pineapple tissue cultivating seedling transplanting medium of embodiment 1); Contrast matrix: river sand matrix, upper strata matrix are river sand, and lower floor's matrix phase of lower floor's matrix and embodiment 1 together; Test matrix is identical with the thickness of contrast matrix.
Test method
Matrix is drenched, standby;
The pineapple tissue cultivating seedling that hardening is handled is heeled in to pineapple tissue cultivating seedling transplanting medium, and each is handled 3 times and repeats, and district's group is arranged at random, and each repeats kind of 300 strains.The normal root water of drenching immediately after transplanting seedlings is looked matrix afterwards and is done wet situation and carry out trickle.Transplant back 30 d and begin fertilising, execute 1 time every 7～10 d.In addition, when temperature of shed surpassed 30 ℃, 10:00～11:00 sheltered from heat or light with the sunshade net the morning, behind the afternoon 16:30 sunshade net was lifted and removed.
That transplants back 30,60,90,120 d investigation pineapple plant newly takes out the number of blade, length of blade and growth indexes such as width, plant height, and in transplanting back 60 d investigation transplanting survival rate situation.In addition, when off-test (transplant back 150 d), indexs such as leaf is wide, plant height long except that the leaf of investigation pineapple plant, also investigate amount of growth indexs such as the total leaf number of plant, the number of taking root, root length, overground part fresh weight and root fresh weight, and adopted the Deng Kenshi duncan's new multiple range method that survey data is carried out statistical analysis.
Result of the test is shown in Fig. 1～4.
Fig. 1 is the figure that influences that newly takes out the number of blade after different substrates is transplanted the pineapple tissue cultivating seedling.From Fig. 1 as can be seen, the pineapple tissue cultivating seedling is transplanted back 30 d, and the plant of peat soil matrix treatments is newly taken out obviously many than the river sand matrix treatments of the number of blade, transplant behind 60 d plant leaf of peat soil matrix take out give birth to faster, more.
Fig. 2 is different substrates is transplanted rear blade length to the pineapple tissue cultivating seedling the figure that influences.From Fig. 2 as can be seen, after the pineapple tissue cultivating seedling was transplanted, the plant leaf length that different cultivation matrixes are handled there are differences.Wherein, transplant back 30 d, the blade of peat soil matrix treatments plant is longer slightly than the blade of river sand matrix; Transplant back 60 d, the long difference of both leaves is not obvious; But along with the prolongation of growth time, the leaf growth of peat soil matrix treatments plant is accelerated, and the leaf growth of river sand matrix plant is slower, and it is extremely remarkable to handle differences.Transplant back 120 d, the leaf length of river sand matrix treatments plant has only 8 cm, and long nearly 17 cm of the leaf of peat soil matrix treatments plant, is about the former 2 times.
Fig. 3 is different substrates is transplanted the rear blade width to the pineapple tissue cultivating seedling the figure that influences.From Fig. 3 as can be seen, the blade of peat soil matrix treatments plant is wide than river sand matrix treatments obviously, and wherein after transplanting before 60 d, the wide growth rate of leaf of peat soil matrix treatments plant is faster than river sand matrix treatments, the wide growth rate of leaf is still variant afterwards, but difference reduces to some extent.
Fig. 4 is different substrates is transplanted the back plant height to the pineapple tissue cultivating seedling the figure that influences.From Fig. 4 as can be seen, after the pineapple tissue cultivating seedling is transplanted, the plant of peat soil matrix treatments increases comparatively fast, and the plant height of river sand matrix treatments plant growth is slower, it is more obvious wherein to transplant behind 60 d both plant height difference, plant height to transplanting back 120 d peat soil matrix treatments plant reaches more than 17 cm, and the plant height of river sand matrix only is about 8 cm.
In the peat soil matrix, 300 strain pineapple tissue cultivating seedling are transplanted back 60 d, survive 289 strains, and survival rate reaches 96.3%; And with in the river sand matrix, 300 strain pineapple tissue cultivating seedling survive 245 strains, and survival rate is 81.7%, and survival rate has improved 14.6%.
After transplanting back 150d, detect the amount of growth of pineapple tissue cultivating seedling, testing result such as following table:
Remarks: total leaf number does not comprise dead leaf and Huang Ye in (1) table; (2) in the table after the same column data small letter English alphabet difference person represent significant difference, capitalization English letter difference person represents that difference is extremely remarkable
From last table as can be known, pineapple tissue cultivating seedling plant height, the total leaf number of transplanting in peat soil matrix, leaf is long, leaf is wide, take root number, overground part fresh weight, root fresh weight etc. are significantly higher than all that (or more than) transplant in river sand matrix, and the long growth difference of root is not remarkable.
As seen, pineapple tissue cultivating seedling transplanting medium of the present invention is used for the pineapple tissue cultivating seedling and transplants, and has survival rate height, root of hair rate height, can significantly shorten advantages such as outplanting time, is to cultivate the preferable transplanting medium of pineapple tissue cultivating seedling.
Claims (3)
1. pineapple tissue cultivating seedling transplanting medium is made of two-layer up and down, and the upper strata is the peat soil layer, and its thickness is 3～4 cm, and lower floor is a mixed layer, is mixed by the mass ratio of 8～6:3～4:1 by yellow mud, husk, peat soil to form, and its thickness is not less than 8 cm.
2. a kind of pineapple tissue cultivating seedling transplanting medium according to claim 1, it is characterized in that: the thickness of mixed layer is 8～9 cm.
3. a kind of pineapple tissue cultivating seedling transplanting medium according to claim 1 is characterized in that: mixed layer is mixed by the mass ratio of 6:4:1 by yellow mud, husk, peat soil and forms.
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| CN2011100425474A CN102172151B (en) | 2011-02-23 | 2011-02-23 | Transplanting matrix for tissue culture seedlings of pineapple |
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| CN102172151B CN102172151B (en) | 2012-08-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381107A (en) * | 2014-11-06 | 2015-03-04 | 海南省农业科学院热带果树研究所 | Hardening-seedling and temporary planting method for Tainong No.16 pineapple tissue culture seedling |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1557142A (en) * | 2004-01-13 | 2004-12-29 | 东莞市林业科学研究所 | Silverback Artocarpus in vitro breeding method |
| CN101278642A (en) * | 2007-04-05 | 2008-10-08 | 上海市农业科学院园艺研究所 | Method for training tissue culture seedling of tomato |
| CN101658138A (en) * | 2009-09-07 | 2010-03-03 | 清流县鸿翔农庄农业发展有限公司 | Optimized substrate for tissue culture, transplantation and hardening-off of gerbera jamesonii |
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- 2011-02-23 CN CN2011100425474A patent/CN102172151B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1557142A (en) * | 2004-01-13 | 2004-12-29 | 东莞市林业科学研究所 | Silverback Artocarpus in vitro breeding method |
| CN101278642A (en) * | 2007-04-05 | 2008-10-08 | 上海市农业科学院园艺研究所 | Method for training tissue culture seedling of tomato |
| CN101658138A (en) * | 2009-09-07 | 2010-03-03 | 清流县鸿翔农庄农业发展有限公司 | Optimized substrate for tissue culture, transplantation and hardening-off of gerbera jamesonii |
Non-Patent Citations (1)
| Title |
|---|
| 《现代农业科技》 20101231 吴雅 铁皮石斛组培苗移栽基质的筛选 107-108,110 1-3 , 第6期 2 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381107A (en) * | 2014-11-06 | 2015-03-04 | 海南省农业科学院热带果树研究所 | Hardening-seedling and temporary planting method for Tainong No.16 pineapple tissue culture seedling |
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