CN101606528A - A kind of soup composition and method of making the same and application of cottage propagation macadamia nut - Google Patents

A kind of soup composition and method of making the same and application of cottage propagation macadamia nut Download PDF

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Publication number
CN101606528A
CN101606528A CNA2009100897798A CN200910089779A CN101606528A CN 101606528 A CN101606528 A CN 101606528A CN A2009100897798 A CNA2009100897798 A CN A2009100897798A CN 200910089779 A CN200910089779 A CN 200910089779A CN 101606528 A CN101606528 A CN 101606528A
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Prior art keywords
macadamia nut
soup
weight portions
cottage propagation
soup composition
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CNA2009100897798A
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CN101606528B (en
Inventor
陆超忠
曾辉
邹明宏
张汉周
杜丽清
罗炼芳
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South Subtropical Crops Research Institute CATAS
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South Subtropical Crops Research Institute CATAS
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Abstract

The invention discloses a kind of by thiophanate methyl, gibberellin, indolebutyric acid, Cobastab 1The soup composition and method of making the same and the application of the cottage propagation macadamia nut of forming; the mode that adopts soup composition of the present invention to transplant a cutting with dipping is used for the macadamia nut cuttage and seedling culture to promote rooting of cuttings; average cuttage planting percent is 96.2%; improve cuttage planting percent 144.3%~164.1%, and healthy and strong neat, the well developed root system of nursery stock, with low cost, easy and simple to handle, be easy to large-scale promotion.

Description

A kind of soup composition and method of making the same and application of cottage propagation macadamia nut
Technical field
The present invention relates to the cottage propagation technology of fruit tree, particularly a kind of macadamia nut cottage propagation technology.
Background technology
Macadamia nut (Macadamia integrifolia) claims the Hawaii fruit again, originates in Australia, is the five-star edible nut in the world, and the good reputation of " king of nut ", " the best edible nut in the world " is arranged.China begins commodity plantation macadamia nut in the later stage 1980s, mainly concentrates on Yunnan and Guangxi.Plantation macadamia nut remarkable in economical benefits is the specialty economies seeds that plant rare suitable China hot-zone.
Macadamia nut adopts the seed growing seedling to have serious genetic variation, and raising seedling of Australian nut mainly adopts grafting both at home and abroad at present.Since macadamia nut wooden crisp hard, skin thin, tanning matter content height, leaf margin tool thorn, the grafting difficulty is big, operating efficiency is lower, conventional grafting seedlings-growing method survival rate is lower, the seed costliness, the seedling cost height is difficult to satisfy and produces needs.
As far back as earlier 1920s, Australia and the Hawaii all cottage propagation of imagination employing once substitute graft seedling growth, but the cuttage seeding root system development that obtains at that time was bad, obtained satisfied plant.Present Australian commercial nursery production cuttage seeding, but at the bottom of all adopting seedbed bottom can heat, keep bed temperature between 24~26.5 ℃, carry out cottage propagation under top can regulate automatically that air themperature is no more than that 28~30 ℃, blade face are equipped with that electronic sensor electrode auto-control spraying system switch, top are equipped with that a cover obscurity is 60%, another obscurity is 30% light modulating device the complex facilities condition.Because facility costliness, complicated operation, China is difficult to promote the use of, and has restricted macadamia nut in the development that should plant the area.
Summary of the invention
The object of the present invention is to provide a kind of soup composition of cottage propagation macadamia nut; Another object of the present invention is to provide a kind of soup preparation method of composition of cottage propagation macadamia nut; A further object of the present invention is to provide a kind of application of soup composition in cottage propagation macadamia nut process of cottage propagation macadamia nut.
The soup composition of a kind of cottage propagation macadamia nut of the present invention comprises the soup that following component and proportioning are made: water 1000000 weight portions, thiophanate methyl 600~1000 weight portions, gibberellin 5~50 weight portions, indolebutyric acid 500~15000 weight portions, Cobastab 110~150 weight portions.
Further, the soup composition of cottage propagation macadamia nut of the present invention comprises following component and proportioning: water 1000000 weight portions, thiophanate methyl 650~875 weight portions, gibberellin 10~30 weight portions, indolebutyric acid 1000~10000 weight portions, Cobastab 120~100 weight portions.
The soup composition of cottage propagation macadamia nut of the present invention, preferably: water 1000000 weight portions, thiophanate methyl 700~800 weight portions, gibberellin 10~20 weight portions, indolebutyric acid 5000~8000 weight portions, Cobastab 140~80 weight portions.Most preferredly be: water 1000000 weight portions, thiophanate methyl 875 weight portions, gibberellin 15 weight portions, indolebutyric acid 7000 weight portions, Cobastab 150 weight portions.
Weight portion of the present invention can be known content units such as mg, g, kg.
The soup preparation of compositions method of cottage propagation macadamia nut of the present invention is: take by weighing following material respectively by proportioning: thiophanate methyl, gibberellin, indolebutyric acid, Cobastab 1, earlier gibberellin, indolebutyric acid are dissolved in an amount of ethanolic solution respectively, again with above-mentioned each lysate and thiophanate methyl, Cobastab 1Surely be dissolved in mixing in the 1L water, promptly get composition.
The using method of the soup composition of cottage propagation macadamia nut of the present invention is that the mode of transplanting a cutting with dipping is used for the macadamia nut cuttage and seedling culture to promote rooting of cuttings.Concrete steps are as follows:
Select the branch of the greyish white suberification of color of the leather, diameter 0.5~0.8cm, be cut into the plugged ear that is about 25cm, plugged ear only stays top one to take turns intact leaf (3), clip is from the about 1.5cm in joint position, top, again with the two-sided wedgewise of cutting sth. askew in plugged ear bottom, two-sided long incision 1~1.5cm, two-sided otch is through the position of celebrating a festival.Earlier place the above-mentioned composition soup to soak for 3~5 seconds the plugged ear notch portion before the cuttage.Other programs and the requirement of cottage propagation are identical with conventional method.
Key problem in technology point of the present invention is:
1, the thiophanate methyl during present composition raw material is formed is to inhale the low toxicity bactericide in a kind of broad spectrum activity, can flow to the top conduction with liquid, disturbs the formation of spindle in the pathogen mitosis, influences the cell division of pathogen, plays bactericidal action.In the macadamia nut cottage propagation, use thiophanate methyl and can reduce pathogen, protect cambial normal activity, thereby improve the cuttage survival rate of macadamia nut cambial harm.It is all very safe to people, animal, natural enemy, plant simultaneously.
Plant growth regulator such as the gibberellin during 2, present composition raw material is formed, indolebutyric acid play significant facilitation to the macadamia nut cuttage rooting, can promote to transplant a cutting the expression with the endogenous hormones effect redistributed of inner nutriment, improve plugged ear Premeabilisation of cells pressure, water absorbing capacity and enzymic activity, make cell finish dedifferentiation and differentiation more smoothly, promote rooting of cuttings.
The optional gibberellic acid of component gibberellin (GA3) of the soup composition of cottage propagation macadamia nut of the present invention.
3, the vitamin B1 during present composition raw material is formed exists with the form of b1thiaminpyrophosphate ester in plant corpus, in the plant cultured in vitro, the variation frequency of the somaclone that is caused by DNA variation is inversely proportional to the frequency of somatic embryo generation, be to cause one of low reason of plant cultured in vitro regeneration rate, and the amido of thiamine with positive charge can be directly in and the DNA negative electrical charge, thereby stabilized DNA structure, the somatic embryo occurrence frequency is increased, realize the totipotency of plant cell expression.
The mode that the soup composition of cottage propagation macadamia nut of the present invention is transplanted a cutting with dipping adopts conventional equipment, and average cuttage planting percent reaches 96.2%, compared with the prior art, improves cuttage planting percent 144.3%~164.1%.And have following advantage:
1, the present invention is under normal condition, the cuttage breeding method of preserve moisture to shade, technological system such as plugged ear processing, cuttage seedbed management being formed a complete and comprehensive system for collecting real estate fees.Compared with the prior art, adopt conventional equipment, heat in the slotting machine bottom that need not external employing, the expensive facility of top temperature control, is convenient to promote.
2, cuttage and seedling culture planting percent height, healthy and strong neat, the well developed root system of nursery stock, technology is grasped easily.
3, being suitable for the extensive cuttage and seedling culture of macadamia nut uses.
Embodiment 1
Take by weighing following material: thiophanate methyl 875mg, gibberellic acid 15mg, indolebutyric acid 7000mg, Cobastab 150mg earlier is dissolved in the 15g ethanolic solution with gibberellic acid, indolebutyric acid is dissolved in the 300g ethanolic solution, again with above-mentioned each lysate and thiophanate methyl, Cobastab 1Surely be dissolved in the preparing tank of 1L water and dissolve, stirring and evenly mixing promptly gets the soup composition.
Select the branch of the greyish white suberification of color of the leather, diameter 0.5~0.8cm, be cut into the plugged ear that is about 25cm, plugged ear only stays top one to take turns intact leaf (3), clip is from the about 1.5cm in joint position, top, again with the two-sided wedgewise of cutting sth. askew in plugged ear bottom, two-sided long incision 1~1.5cm, two-sided otch is through the position of celebrating a festival.With the plugged ear notch portion, place the above-mentioned composition soup to soak for 3~5 seconds before the cuttage.Other programs and the requirement of cottage propagation are identical with conventional method.
Use above-mentioned soup composition to be applied to the macadamia nut graft seedling growth, average cuttage planting percent is 96.2%, and 37.6% of comparison photograph has improved 155.9%, through the t check, handles and compares and reach difference utmost point significance level.
Embodiment 2
Take by weighing following material: thiophanate methyl 750mg, gibberellic acid 10mg, indolebutyric acid 6000mg, Cobastab 140mg earlier is dissolved in the 20g ethanolic solution with gibberellic acid, indolebutyric acid is dissolved in the 300g ethanolic solution, again with above-mentioned each lysate and thiophanate methyl, Cobastab 1Surely be dissolved in the preparing tank of 1L water and dissolve, stirring and evenly mixing promptly gets the soup composition.
Select the branch of the greyish white suberification of color of the leather, diameter 0.5~0.8cm, be cut into the plugged ear that is about 25cm, plugged ear only stays top one to take turns intact leaf (3), clip is from the about 1.5cm in joint position, top, again with the two-sided wedgewise of cutting sth. askew in plugged ear bottom, two-sided long incision 1~1.5cm, two-sided otch is through the position of celebrating a festival.With the plugged ear notch portion, place the above-mentioned composition soup to soak for 3~5 seconds before the cuttage.Other programs and the requirement of cottage propagation are identical with conventional method.
Use above-mentioned soup composition to be applied to the macadamia nut cuttage and seedling culture, average cuttage planting percent is 81.8%.
Embodiment 3
Take by weighing following material: thiophanate methyl 600mg, gibberellic acid 5mg, indolebutyric acid 500mg, Cobastab 110mg earlier is dissolved in the 10g ethanolic solution with gibberellic acid, indolebutyric acid is dissolved in the 50g ethanolic solution, again with above-mentioned each lysate and thiophanate methyl, Cobastab 1Surely be dissolved in the preparing tank of 1L water and dissolve, stirring and evenly mixing promptly gets the soup composition.
Select the branch of the greyish white suberification of color of the leather, diameter 0.5~0.8cm, be cut into the plugged ear that is about 25cm, plugged ear only stays top one to take turns intact leaf (3), clip is from the about 1.5cm in joint position, top, again with the two-sided wedgewise of cutting sth. askew in plugged ear bottom, two-sided long incision 1~1.5cm, two-sided otch is through the position of celebrating a festival.With the plugged ear notch portion, place the above-mentioned composition soup to soak for 3~5 seconds before the cuttage.Other programs and the requirement of cottage propagation are identical with conventional method.
Use above-mentioned soup composition to be applied to the macadamia nut cuttage and seedling culture, average cuttage planting percent is 40.1%.
Embodiment 4
Take by weighing following material: thiophanate methyl 1000mg, gibberellic acid 50mg, indolebutyric acid 15000mg, Cobastab 1150mg earlier is dissolved in the 50g ethanolic solution with gibberellic acid, indolebutyric acid is dissolved in the 500g ethanolic solution, again with above-mentioned each lysate and thiophanate methyl, Cobastab 1Surely be dissolved in the preparing tank of 1L water and dissolve, stirring and evenly mixing promptly gets the soup composition.
Select the branch of the greyish white suberification of color of the leather, diameter 0.5~0.8cm, be cut into the plugged ear that is about 25cm, plugged ear only stays top one to take turns intact leaf (3), clip is from the about 1.5cm in joint position, top, again with the two-sided wedgewise of cutting sth. askew in plugged ear bottom, two-sided long incision 1~1.5cm, two-sided otch is through the position of celebrating a festival.With the plugged ear notch portion, place the above-mentioned composition soup to soak for 3~5 seconds before the cuttage.Other programs and the requirement of cottage propagation are identical with conventional method.
Use above-mentioned soup composition to be applied to the macadamia nut cuttage and seedling culture, average cuttage planting percent is 29.8%.

Claims (4)

1, a kind of soup composition of cottage propagation macadamia nut, this soup composition comprises the soup that following component and proportioning are made: water 1000000 weight portions, thiophanate methyl 600~1000 weight portions, gibberellin 5~50 weight portions, indolebutyric acid 500~15000 weight portions, Cobastab 110~150 weight portions.
2, the soup composition of a kind of cottage propagation macadamia nut according to claim 1 is characterized in that this soup preparation of compositions method is to take by weighing following material respectively by proportioning: thiophanate methyl, gibberellin, indolebutyric acid, Cobastab 1, earlier with gibberellin, indolebutyric acid is dissolved in adequate amount of ethanol solution respectively, again with above-mentioned each lysate and thiophanate methyl, Cobastab 1Surely be dissolved in mixing in the 1L water.
3,, it is characterized in that the two-sided otch in described plugged ear bottom is through the position wedgewise of cutting sth. askew of celebrating a festival according to the soup composition of right 1 or 2 described a kind of cottage propagation macadamia nuts.
4, use the soup method for compositions of the described a kind of propagation by grafiting macadamia nut of arbitrary claim in the claim 1 or 2, it is characterized in that the mode that described soup composition is transplanted a cutting with dipping is used for the macadamia nut cuttage and seedling culture.
CN 200910089779 2009-07-23 2009-07-23 Drug liquor composition for cutting and reproducing macadimia nut, preparation method and application thereof Expired - Fee Related CN101606528B (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102613009A (en) * 2012-03-29 2012-08-01 常熟市佳盛农业科技发展有限公司 Special nutrient solution for grafting trees
CN102793169A (en) * 2012-08-27 2012-11-28 中国热带农业科学院农产品加工研究所 Macadamia nut oil dropping pill and preparation method thereof
CN103553778A (en) * 2013-10-25 2014-02-05 遵义师范学院 Cephalotaxus fortune cutting seedling rooting-promoting compound conditioner and preparation method thereof
CN104429433A (en) * 2013-11-25 2015-03-25 邱逸奎 Uncaria cuttage method
CN104838952A (en) * 2015-05-25 2015-08-19 安徽省怀远县华夏药械有限责任公司 Catalpa bungei seedling cultivation method
CN104920078A (en) * 2015-06-10 2015-09-23 岑溪市新丰农业综合开发有限公司 Method for quickly rearing macadamia nut grafted seedlings through plantlets
CN105432403A (en) * 2015-12-01 2016-03-30 广西合山市润鸿农业技术有限公司 Quick cultivation method for macadimia nut seedlings
CN106489493A (en) * 2016-10-25 2017-03-15 广西科技大学鹿山学院 A kind of method for improving macadimia nut cuttage shoot survival percent

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1349739A (en) * 2001-11-23 2002-05-22 北海市立方生态技术有限公司 Rooting agent suitable for raising seedling of Australian nut

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102613009A (en) * 2012-03-29 2012-08-01 常熟市佳盛农业科技发展有限公司 Special nutrient solution for grafting trees
CN102793169A (en) * 2012-08-27 2012-11-28 中国热带农业科学院农产品加工研究所 Macadamia nut oil dropping pill and preparation method thereof
CN102793169B (en) * 2012-08-27 2014-04-02 中国热带农业科学院农产品加工研究所 Macadamia nut oil dropping pill and preparation method thereof
CN103553778A (en) * 2013-10-25 2014-02-05 遵义师范学院 Cephalotaxus fortune cutting seedling rooting-promoting compound conditioner and preparation method thereof
CN103553778B (en) * 2013-10-25 2015-11-18 遵义师范学院 A kind of Cephalotaxus fortunei cutting propagation growth-promoting root composite regulator and preparation method thereof
CN104429433A (en) * 2013-11-25 2015-03-25 邱逸奎 Uncaria cuttage method
CN104838952A (en) * 2015-05-25 2015-08-19 安徽省怀远县华夏药械有限责任公司 Catalpa bungei seedling cultivation method
CN104920078A (en) * 2015-06-10 2015-09-23 岑溪市新丰农业综合开发有限公司 Method for quickly rearing macadamia nut grafted seedlings through plantlets
CN105432403A (en) * 2015-12-01 2016-03-30 广西合山市润鸿农业技术有限公司 Quick cultivation method for macadimia nut seedlings
CN106489493A (en) * 2016-10-25 2017-03-15 广西科技大学鹿山学院 A kind of method for improving macadimia nut cuttage shoot survival percent

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