CN101348780B - 从链霉蛋白酶中分离和纯化胰蛋白酶的方法及其用途 - Google Patents

从链霉蛋白酶中分离和纯化胰蛋白酶的方法及其用途 Download PDF

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CN101348780B
CN101348780B CN200810137978.7A CN200810137978A CN101348780B CN 101348780 B CN101348780 B CN 101348780B CN 200810137978 A CN200810137978 A CN 200810137978A CN 101348780 B CN101348780 B CN 101348780B
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purifying
cell
virus
pronase
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CN101348780A (zh
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A·米特雷尔
C·陶尔
M·赖特尔
W·蒙德特
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Baxter Co
Nanomedicine
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Baxter Healthcare SA
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32411—Hepatovirus, i.e. hepatitis A virus
    • C12N2770/32451—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN200810137978.7A 2001-12-10 2002-12-10 从链霉蛋白酶中分离和纯化胰蛋白酶的方法及其用途 Expired - Fee Related CN101348780B (zh)

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Application Number Priority Date Filing Date Title
US10/006223 2001-12-10
US10/006,223 US6830917B2 (en) 2001-12-10 2001-12-10 Method of isolation and purification of trypsin from pronase protease and use thereof

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CN02827638.8A Division CN1617882B (zh) 2001-12-10 2002-12-10 从链霉蛋白酶中分离和纯化胰蛋白酶的方法及其用途

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CN101348780A CN101348780A (zh) 2009-01-21
CN101348780B true CN101348780B (zh) 2013-02-06

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CN02827638.8A Expired - Fee Related CN1617882B (zh) 2001-12-10 2002-12-10 从链霉蛋白酶中分离和纯化胰蛋白酶的方法及其用途

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US (4) US6830917B2 (enExample)
EP (1) EP1456228B1 (enExample)
JP (2) JP4629336B2 (enExample)
CN (2) CN101348780B (enExample)
AT (1) ATE508137T1 (enExample)
AU (2) AU2002363865B2 (enExample)
CA (1) CA2469484C (enExample)
DE (1) DE60239957D1 (enExample)
DK (1) DK1456228T3 (enExample)
MX (1) MXPA04005639A (enExample)
NO (1) NO335427B1 (enExample)
WO (1) WO2003050275A2 (enExample)

Families Citing this family (6)

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KR100687884B1 (ko) 2005-12-30 2007-02-27 한국과학기술원 스트렙토마이시스 그리세우스 유래의 조절유전자 ｓｇｔＲ1및 ｓｇｔＲ2를 이용한 재조합 트립신의 대량 생산방법
JP6021218B2 (ja) * 2012-06-25 2016-11-09 多木化学株式会社 コラーゲン誘導体の製造方法
UA120259C2 (uk) 2013-11-08 2019-11-11 Пресіжн Плентінг Елелсі Система керування вагою сільськогосподарського знаряддя
CN108333358B (zh) * 2017-01-19 2020-05-01 深圳市新产业生物医学工程股份有限公司 一种免疫亲和纯化洗脱液的筛选方法
CN112143693B (zh) 2019-06-28 2024-12-27 杭州康万达医药科技有限公司 一种生产病毒的方法及收获液组合物
CN110343689B (zh) * 2019-08-23 2021-11-05 四川大学 一种链霉菌胰蛋白酶gm2938及其在枯草芽孢杆菌中的异源表达

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4973554A (en) * 1987-05-29 1990-11-27 National Research Council Of Canada Affinity process for trypsin purification and stabilization

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SE445466B (sv) * 1977-12-01 1986-06-23 Wellcome Found Sett att foroka rotavirus in vitro
CA1122527A (en) * 1979-05-15 1982-04-27 Karen K. Brown Influenza vaccine production in liquid cell culture
US4500513A (en) * 1979-05-15 1985-02-19 Miles Laboratories, Inc. Influenza vaccine production in liquid cell culture
JPH02268681A (ja) * 1989-04-07 1990-11-02 Green Cross Corp:The ウロキナーゼ前駆体の安定化方法及び乾燥製剤
RU2082431C1 (ru) * 1993-05-12 1997-06-27 Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им.Пастера Способ получения инактивированной гриппозной вакцины
US5824536A (en) * 1994-08-23 1998-10-20 St. Jude Children's Research Hospital Influenza virus replicated in mammalian cell culture and vaccine production
ES2323490T3 (es) 1994-11-10 2009-07-17 Baxter Healthcare Sa Procedimiento de produccion de agentes biologicos en un medio de cultivo sin proteinas.
US5989805A (en) * 1995-10-27 1999-11-23 Board Of Trustees Operating Michigan State University Immortal avian cell line to grow avian and animal viruses to produce vaccines
AT404597B (de) 1995-11-24 1998-12-28 Immuno Ag Verfahren zur herstellung von proteinen
US5905042A (en) * 1996-04-01 1999-05-18 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cultured inner cell mass cell lines derived from bovine or porcine embryos
US6168944B1 (en) * 1997-01-31 2001-01-02 Schering Corporation Methods for cultivating cells and propagating viruses
AT407255B (de) 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons
ES2435141T3 (es) * 1999-02-22 2013-12-18 University Of Connecticut Nuevas formulaciones de factor VIII libres de albúmina
US20030032146A1 (en) * 2001-07-10 2003-02-13 Dwulet Francis Edward Enzyme/tag binding and detection system
US6825027B2 (en) * 2001-12-10 2004-11-30 Baxter Healthcare S.A. Method of production of purified hepatitis a virus particles and vaccine preparation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4973554A (en) * 1987-05-29 1990-11-27 National Research Council Of Canada Affinity process for trypsin purification and stabilization

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Ken-ichi KASAI et al.Affinity Chromatography of Trypsin and Related Enzymes.《J.Biochem》.1978,第84卷1051-1060. *
潘继承等.固定化胰蛋白酶的制备及其性质研究.《湖北师范学院学报(自然科学版)》.2001,第21卷(第1期),50-53. *
马建农等.胰酶中多种蛋白水解酶的分离和纯化.《医药工业》.1988,第19卷(第19期),385-390. *

Also Published As

Publication number Publication date
CN1617882B (zh) 2010-05-12
AU2002363865A1 (en) 2003-06-23
NO20042908L (no) 2004-07-09
DE60239957D1 (de) 2011-06-16
EP1456228A2 (en) 2004-09-15
US20030124650A1 (en) 2003-07-03
ATE508137T1 (de) 2011-05-15
MXPA04005639A (es) 2005-04-19
AU2008229752B2 (en) 2011-02-17
JP2005511087A (ja) 2005-04-28
CA2469484A1 (en) 2003-06-19
US20050069534A1 (en) 2005-03-31
US6830917B2 (en) 2004-12-14
WO2003050275A8 (en) 2004-05-21
US20120003691A1 (en) 2012-01-05
DK1456228T3 (da) 2011-08-15
CN101348780A (zh) 2009-01-21
US20070249015A1 (en) 2007-10-25
US7223595B2 (en) 2007-05-29
WO2003050275A3 (en) 2004-02-19
EP1456228B1 (en) 2011-05-04
CA2469484C (en) 2013-09-10
US8389261B2 (en) 2013-03-05
WO2003050275A2 (en) 2003-06-19
AU2008229752A1 (en) 2008-10-30
NO335427B1 (no) 2014-12-15
US7659380B2 (en) 2010-02-09
CN1617882A (zh) 2005-05-18
AU2002363865B2 (en) 2008-07-03
JP2010268799A (ja) 2010-12-02
JP4629336B2 (ja) 2011-02-09

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