AU2002363865B2 - Method of isolation and purification of trypsin from pronase and use thereof - Google Patents
Method of isolation and purification of trypsin from pronase and use thereof Download PDFInfo
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- AU2002363865B2 AU2002363865B2 AU2002363865A AU2002363865A AU2002363865B2 AU 2002363865 B2 AU2002363865 B2 AU 2002363865B2 AU 2002363865 A AU2002363865 A AU 2002363865A AU 2002363865 A AU2002363865 A AU 2002363865A AU 2002363865 B2 AU2002363865 B2 AU 2002363865B2
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- trypsin
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Classifications
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16051—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16151—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/32011—Picornaviridae
- C12N2770/32411—Hepatovirus, i.e. hepatitis A virus
- C12N2770/32451—Methods of production or purification of viral material
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
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- Biomedical Technology (AREA)
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- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Virology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2008229752A AU2008229752B2 (en) | 2001-12-10 | 2008-10-02 | Method of Isolation and Purification of Trypsin from Pronase and Use Thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/006,223 US6830917B2 (en) | 2001-12-10 | 2001-12-10 | Method of isolation and purification of trypsin from pronase protease and use thereof |
| US10/006,223 | 2001-12-10 | ||
| PCT/EP2002/014005 WO2003050275A2 (en) | 2001-12-10 | 2002-12-10 | Method of isolation and purification of trypsin from pronase and use thereof |
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| AU2002363865A1 AU2002363865A1 (en) | 2003-06-23 |
| AU2002363865B2 true AU2002363865B2 (en) | 2008-07-03 |
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| AU2008229752A Ceased AU2008229752B2 (en) | 2001-12-10 | 2008-10-02 | Method of Isolation and Purification of Trypsin from Pronase and Use Thereof |
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| AU2008229752A Ceased AU2008229752B2 (en) | 2001-12-10 | 2008-10-02 | Method of Isolation and Purification of Trypsin from Pronase and Use Thereof |
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| EP (1) | EP1456228B1 (enExample) |
| JP (2) | JP4629336B2 (enExample) |
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| AT (1) | ATE508137T1 (enExample) |
| AU (2) | AU2002363865B2 (enExample) |
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| DE (1) | DE60239957D1 (enExample) |
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| MX (1) | MXPA04005639A (enExample) |
| NO (1) | NO335427B1 (enExample) |
| WO (1) | WO2003050275A2 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100687884B1 (ko) | 2005-12-30 | 2007-02-27 | 한국과학기술원 | 스트렙토마이시스 그리세우스 유래의 조절유전자 ｓｇｔＲ1및 ｓｇｔＲ2를 이용한 재조합 트립신의 대량 생산방법 |
| JP6021218B2 (ja) * | 2012-06-25 | 2016-11-09 | 多木化学株式会社 | コラーゲン誘導体の製造方法 |
| UA120259C2 (uk) | 2013-11-08 | 2019-11-11 | Пресіжн Плентінг Елелсі | Система керування вагою сільськогосподарського знаряддя |
| CN108333358B (zh) * | 2017-01-19 | 2020-05-01 | 深圳市新产业生物医学工程股份有限公司 | 一种免疫亲和纯化洗脱液的筛选方法 |
| CN112143693B (zh) | 2019-06-28 | 2024-12-27 | 杭州康万达医药科技有限公司 | 一种生产病毒的方法及收获液组合物 |
| CN110343689B (zh) * | 2019-08-23 | 2021-11-05 | 四川大学 | 一种链霉菌胰蛋白酶gm2938及其在枯草芽孢杆菌中的异源表达 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4973554A (en) * | 1987-05-29 | 1990-11-27 | National Research Council Of Canada | Affinity process for trypsin purification and stabilization |
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| SE445466B (sv) * | 1977-12-01 | 1986-06-23 | Wellcome Found | Sett att foroka rotavirus in vitro |
| CA1122527A (en) * | 1979-05-15 | 1982-04-27 | Karen K. Brown | Influenza vaccine production in liquid cell culture |
| US4500513A (en) * | 1979-05-15 | 1985-02-19 | Miles Laboratories, Inc. | Influenza vaccine production in liquid cell culture |
| JPH02268681A (ja) * | 1989-04-07 | 1990-11-02 | Green Cross Corp:The | ウロキナーゼ前駆体の安定化方法及び乾燥製剤 |
| RU2082431C1 (ru) * | 1993-05-12 | 1997-06-27 | Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им.Пастера | Способ получения инактивированной гриппозной вакцины |
| US5824536A (en) * | 1994-08-23 | 1998-10-20 | St. Jude Children's Research Hospital | Influenza virus replicated in mammalian cell culture and vaccine production |
| ES2323490T3 (es) | 1994-11-10 | 2009-07-17 | Baxter Healthcare Sa | Procedimiento de produccion de agentes biologicos en un medio de cultivo sin proteinas. |
| US5989805A (en) * | 1995-10-27 | 1999-11-23 | Board Of Trustees Operating Michigan State University | Immortal avian cell line to grow avian and animal viruses to produce vaccines |
| AT404597B (de) | 1995-11-24 | 1998-12-28 | Immuno Ag | Verfahren zur herstellung von proteinen |
| US5905042A (en) * | 1996-04-01 | 1999-05-18 | University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus | Cultured inner cell mass cell lines derived from bovine or porcine embryos |
| US6168944B1 (en) * | 1997-01-31 | 2001-01-02 | Schering Corporation | Methods for cultivating cells and propagating viruses |
| AT407255B (de) | 1997-06-20 | 2001-02-26 | Immuno Ag | Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons |
| ES2435141T3 (es) * | 1999-02-22 | 2013-12-18 | University Of Connecticut | Nuevas formulaciones de factor VIII libres de albúmina |
| US20030032146A1 (en) * | 2001-07-10 | 2003-02-13 | Dwulet Francis Edward | Enzyme/tag binding and detection system |
| US6825027B2 (en) * | 2001-12-10 | 2004-11-30 | Baxter Healthcare S.A. | Method of production of purified hepatitis a virus particles and vaccine preparation |
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2001
- 2001-12-10 US US10/006,223 patent/US6830917B2/en not_active Expired - Lifetime
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2002
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- 2002-12-10 CN CN02827638.8A patent/CN1617882B/zh not_active Expired - Fee Related
- 2002-12-10 WO PCT/EP2002/014005 patent/WO2003050275A2/en not_active Ceased
- 2002-12-10 DK DK02798324.6T patent/DK1456228T3/da active
- 2002-12-10 JP JP2003551296A patent/JP4629336B2/ja not_active Expired - Fee Related
- 2002-12-10 AT AT02798324T patent/ATE508137T1/de active
- 2002-12-10 CA CA2469484A patent/CA2469484C/en not_active Expired - Fee Related
- 2002-12-10 MX MXPA04005639A patent/MXPA04005639A/es active IP Right Grant
- 2002-12-10 AU AU2002363865A patent/AU2002363865B2/en not_active Ceased
- 2002-12-10 DE DE60239957T patent/DE60239957D1/de not_active Expired - Lifetime
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4973554A (en) * | 1987-05-29 | 1990-11-27 | National Research Council Of Canada | Affinity process for trypsin purification and stabilization |
Non-Patent Citations (4)
| Title |
|---|
| ELLOUALI et al. J CHROMATOGR., 1991, vol. 548, no. 1-2, pp. 255-266 * |
| KASAI et al. J BIOCHEM., 1978, vol. 84, no. 5, pp. 1051-1060 * |
| KOO et al. J MICROBIOL BIOTECHNOL., 1998, vol. 8, no. 4, pp. 333-340. * |
| VIVARAT-PERRIN et al. J CHROMATOGR., 1992, vol. 584, no. 1, pp. 3-9 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1617882B (zh) | 2010-05-12 |
| AU2002363865A1 (en) | 2003-06-23 |
| NO20042908L (no) | 2004-07-09 |
| DE60239957D1 (de) | 2011-06-16 |
| EP1456228A2 (en) | 2004-09-15 |
| US20030124650A1 (en) | 2003-07-03 |
| ATE508137T1 (de) | 2011-05-15 |
| MXPA04005639A (es) | 2005-04-19 |
| AU2008229752B2 (en) | 2011-02-17 |
| JP2005511087A (ja) | 2005-04-28 |
| CN101348780B (zh) | 2013-02-06 |
| CA2469484A1 (en) | 2003-06-19 |
| US20050069534A1 (en) | 2005-03-31 |
| US6830917B2 (en) | 2004-12-14 |
| WO2003050275A8 (en) | 2004-05-21 |
| US20120003691A1 (en) | 2012-01-05 |
| DK1456228T3 (da) | 2011-08-15 |
| CN101348780A (zh) | 2009-01-21 |
| US20070249015A1 (en) | 2007-10-25 |
| US7223595B2 (en) | 2007-05-29 |
| WO2003050275A3 (en) | 2004-02-19 |
| EP1456228B1 (en) | 2011-05-04 |
| CA2469484C (en) | 2013-09-10 |
| US8389261B2 (en) | 2013-03-05 |
| WO2003050275A2 (en) | 2003-06-19 |
| AU2008229752A1 (en) | 2008-10-30 |
| NO335427B1 (no) | 2014-12-15 |
| US7659380B2 (en) | 2010-02-09 |
| CN1617882A (zh) | 2005-05-18 |
| JP2010268799A (ja) | 2010-12-02 |
| JP4629336B2 (ja) | 2011-02-09 |
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