CN101218247A - 利用hcic和离子交换层析的蛋白质纯化 - Google Patents

利用hcic和离子交换层析的蛋白质纯化 Download PDF

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Publication number
CN101218247A
CN101218247A CNA2006800166104A CN200680016610A CN101218247A CN 101218247 A CN101218247 A CN 101218247A CN A2006800166104 A CNA2006800166104 A CN A2006800166104A CN 200680016610 A CN200680016610 A CN 200680016610A CN 101218247 A CN101218247 A CN 101218247A
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antibody
target protein
chromatography
electric charge
mixture
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English (en)
Chinese (zh)
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A·阿鲁纳库马里
G·M·M·费赖拉
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ER Squibb and Sons LLC
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Medarex LLC
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39516—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum from serum, plasma
    • A61K39/39525—Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNA2006800166104A 2005-04-11 2006-03-21 利用hcic和离子交换层析的蛋白质纯化 Pending CN101218247A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67045705P 2005-04-11 2005-04-11
US60/670,457 2005-04-11

Publications (1)

Publication Number Publication Date
CN101218247A true CN101218247A (zh) 2008-07-09

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CNA2006800166104A Pending CN101218247A (zh) 2005-04-11 2006-03-21 利用hcic和离子交换层析的蛋白质纯化

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Country Link
US (3) US8129508B2 (enExample)
EP (1) EP1869067A1 (enExample)
JP (1) JP2008535913A (enExample)
KR (1) KR20080006601A (enExample)
CN (1) CN101218247A (enExample)
AU (1) AU2006234909A1 (enExample)
CA (1) CA2604877A1 (enExample)
EA (1) EA013504B1 (enExample)
MX (1) MX2007012499A (enExample)
NZ (1) NZ562949A (enExample)
WO (1) WO2006110277A1 (enExample)

Cited By (9)

* Cited by examiner, † Cited by third party
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CN103028376A (zh) * 2011-09-29 2013-04-10 佛山市博新生物科技有限公司 一种用于清除血液毒素的血液净化吸附剂及其制备方法
CN105777896A (zh) * 2015-03-19 2016-07-20 广东东阳光药业有限公司 一种抗体酸性峰的纯化方法
CN107413312A (zh) * 2017-07-18 2017-12-01 天津工业大学 一种用于抗体纯化的混合模式蛋白吸附膜及制备方法
CN107922476A (zh) * 2015-03-13 2018-04-17 诺夫免疫股份有限公司 纯化双特异性抗体的方法
CN108239146A (zh) * 2018-03-26 2018-07-03 江苏中新医药有限公司 一种高纯度rhNGF的制备方法
CN110348090A (zh) * 2019-06-28 2019-10-18 浙江大学 基于人工神经网络实现多柱连续流层析设计及分析的方法
CN111712510A (zh) * 2017-12-21 2020-09-25 建新公司 用于在蛋白a色谱法期间增强杂质去除的方法
CN111991571A (zh) * 2020-08-12 2020-11-27 湖州师范学院 一种柱上低pH病毒灭活的方法
CN119113574A (zh) * 2024-09-10 2024-12-13 宝利化（南京）制药有限公司 一种熊去氧胆酸制备纯化装置、方法及其应用

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CN102177237B (zh) * 2008-09-12 2013-10-30 金沃特公司 用于贮存和稳定生物分子的基质和介质
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CN102532254B (zh) * 2010-12-24 2015-06-24 武汉禾元生物科技股份有限公司 一种从水稻种子中分离纯化重组人血清白蛋白的方法
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KR20150043523A (ko) 2012-09-02 2015-04-22 애브비 인코포레이티드 단백질 불균일성의 제어 방법
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KR101569783B1 (ko) 2013-06-05 2015-11-19 한화케미칼 주식회사 항체의 정제 방법
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JP6602765B2 (ja) * 2013-09-05 2019-11-06 ジェネンテック， インコーポレイテッド クロマトグラフィー再使用のための方法
RU2016107435A (ru) 2013-09-13 2017-10-18 Дженентек, Инк. Композиции и способы обнаружения и количественного определения белка клеток-хозяев в клеточных линиях и рекомбинантные полипептидные продукты
EP3683232A1 (en) 2013-09-13 2020-07-22 F. Hoffmann-La Roche AG Methods and compositions comprising purified recombinant polypeptides
JPWO2015041218A1 (ja) 2013-09-17 2017-03-02 株式会社カネカ 新規抗体精製方法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎＭｅｔｈｏｄａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎｍｅｔｈｏｄｕｓｉｎｇＣａｔｉｏｎＥｘｃｈａｎｇｅｒａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）
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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103028376A (zh) * 2011-09-29 2013-04-10 佛山市博新生物科技有限公司 一种用于清除血液毒素的血液净化吸附剂及其制备方法
CN114249831A (zh) * 2015-03-13 2022-03-29 诺夫免疫股份有限公司 纯化双特异性抗体的方法
CN107922476A (zh) * 2015-03-13 2018-04-17 诺夫免疫股份有限公司 纯化双特异性抗体的方法
CN105777896B (zh) * 2015-03-19 2019-08-16 广东东阳光药业有限公司 一种抗体酸性峰的纯化方法
CN105777896A (zh) * 2015-03-19 2016-07-20 广东东阳光药业有限公司 一种抗体酸性峰的纯化方法
CN107413312A (zh) * 2017-07-18 2017-12-01 天津工业大学 一种用于抗体纯化的混合模式蛋白吸附膜及制备方法
CN111712510A (zh) * 2017-12-21 2020-09-25 建新公司 用于在蛋白a色谱法期间增强杂质去除的方法
CN111712510B (zh) * 2017-12-21 2024-06-14 建新公司 用于在蛋白a色谱法期间增强杂质去除的方法
US12528835B2 (en) 2017-12-21 2026-01-20 Genzyme Corporation Methods for enhanced removal of impurities during protein a chromatography
CN108239146A (zh) * 2018-03-26 2018-07-03 江苏中新医药有限公司 一种高纯度rhNGF的制备方法
CN110348090A (zh) * 2019-06-28 2019-10-18 浙江大学 基于人工神经网络实现多柱连续流层析设计及分析的方法
CN111991571A (zh) * 2020-08-12 2020-11-27 湖州师范学院 一种柱上低pH病毒灭活的方法
CN119113574A (zh) * 2024-09-10 2024-12-13 宝利化（南京）制药有限公司 一种熊去氧胆酸制备纯化装置、方法及其应用
CN119113574B (zh) * 2024-09-10 2025-04-18 宝利化（南京）制药有限公司 一种熊去氧胆酸制备纯化装置、方法及其应用

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US20140179904A1 (en) 2014-06-26
NZ562949A (en) 2009-05-31
WO2006110277A1 (en) 2006-10-19
EA013504B1 (ru) 2010-06-30
MX2007012499A (es) 2007-12-06
KR20080006601A (ko) 2008-01-16
US8129508B2 (en) 2012-03-06
US8697847B2 (en) 2014-04-15
EA200702210A1 (ru) 2008-04-28
EP1869067A1 (en) 2007-12-26
US20090105465A1 (en) 2009-04-23
AU2006234909A1 (en) 2006-10-19
JP2008535913A (ja) 2008-09-04
US20120165511A1 (en) 2012-06-28
CA2604877A1 (en) 2006-10-19

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