JP2008535913A - タンパク質精製方法 - Google Patents

タンパク質精製方法 Download PDF

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Publication number
JP2008535913A
JP2008535913A JP2008506476A JP2008506476A JP2008535913A JP 2008535913 A JP2008535913 A JP 2008535913A JP 2008506476 A JP2008506476 A JP 2008506476A JP 2008506476 A JP2008506476 A JP 2008506476A JP 2008535913 A JP2008535913 A JP 2008535913A
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Prior art keywords
protein
target protein
antibody
range
chromatography
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Japanese (ja)
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JP2008535913A5 (enExample
Inventor
アラハリ アルナクマリ
ギゼラ マリア マルケス フェレイラ
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ER Squibb and Sons LLC
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Medarex LLC
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Publication of JP2008535913A publication Critical patent/JP2008535913A/ja
Publication of JP2008535913A5 publication Critical patent/JP2008535913A5/ja
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39516—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum from serum, plasma
    • A61K39/39525—Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2008506476A 2005-04-11 2006-03-21 タンパク質精製方法 Withdrawn JP2008535913A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67045705P 2005-04-11 2005-04-11
PCT/US2006/010412 WO2006110277A1 (en) 2005-04-11 2006-03-21 Protein purification using hcic amd ion exchange chromatography

Publications (2)

Publication Number Publication Date
JP2008535913A true JP2008535913A (ja) 2008-09-04
JP2008535913A5 JP2008535913A5 (enExample) 2009-04-23

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ID=36764783

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JP2008506476A Withdrawn JP2008535913A (ja) 2005-04-11 2006-03-21 タンパク質精製方法

Country Status (11)

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US (3) US8129508B2 (enExample)
EP (1) EP1869067A1 (enExample)
JP (1) JP2008535913A (enExample)
KR (1) KR20080006601A (enExample)
CN (1) CN101218247A (enExample)
AU (1) AU2006234909A1 (enExample)
CA (1) CA2604877A1 (enExample)
EA (1) EA013504B1 (enExample)
MX (1) MX2007012499A (enExample)
NZ (1) NZ562949A (enExample)
WO (1) WO2006110277A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015041218A1 (ja) 2013-09-17 2015-03-26 株式会社カネカ 新規抗体精製方法及びそれから得られる抗体（Ｎｏｖｅｌ Ａｎｔｉｂｏｄｙ Ｐｕｒｉｆｉｃａｔｉｏｎ Ｍｅｔｈｏｄ ａｎｄ Ａｎｔｉｂｏｄｙ ｏｂｔａｉｎｅｄ ｔｈｅｒｅｆｒｏｍ）、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体（Ｎｏｖｅｌ Ａｎｔｉｂｏｄｙ Ｐｕｒｉｆｉｃａｔｉｏｎ ｍｅｔｈｏｄ ｕｓｉｎｇ Ｃａｔｉｏｎ Ｅｘｃｈａｎｇｅｒ ａｎｄ Ａｎｔｉｂｏｄｙ ｏｂｔａｉｎｅｄ ｔｈｅｒｅｆｒｏｍ）
JP2016512957A (ja) * 2013-02-22 2016-05-12 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ 負帯電粒子を用いるウイルス調製物のクロマトグラフィー精製
JP2016531304A (ja) * 2013-09-13 2016-10-06 ジェネンテック， インコーポレイテッド 細胞株中の宿主細胞タンパク質及び組換えポリペプチド産物を検出及び定量化するための組成物並びに方法
WO2020067027A1 (ja) * 2018-09-28 2020-04-02 一般財団法人阪大微生物病研究会 Ｖｌｐ発現ｃｈｏ細胞株の構築

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8129508B2 (en) 2005-04-11 2012-03-06 Medarex, Inc. Protein purification using HCIC and ion exchange chromatography
EP2738179A1 (en) 2006-04-05 2014-06-04 AbbVie Biotechnology Ltd Antibody purification
US8513393B2 (en) * 2006-08-28 2013-08-20 Ares Trading S.A. Process for the purification of Fc-containing proteins
FR2910786B1 (fr) * 2006-12-29 2017-08-11 Laboratoire Francais Du Fractionnement Et Des Biotechnologies (Lfb) "procede d'extraction d'une proteine presente dans du lait"
US20100062512A1 (en) * 2007-01-04 2010-03-11 Crucell Holland B.V. Purification of factor xi
KR100955470B1 (ko) 2008-01-09 2010-04-30 대한민국(관리부서 질병관리본부장) 탄저 방어 항원의 제조 방법
CN102177237B (zh) * 2008-09-12 2013-10-30 金沃特公司 用于贮存和稳定生物分子的基质和介质
TW201024318A (en) 2008-10-20 2010-07-01 Abbott Lab Isolation and purification of antibodies using protein A affinity chromatography
SG195577A1 (en) 2008-10-20 2013-12-30 Abbott Lab Viral inactivation during purification of antibodies
EP2346901A1 (en) * 2008-10-20 2011-07-27 Abbott Laboratories Antibodies that bind to il-18 and methods of purifying the same
CA2739352C (en) * 2008-10-29 2021-07-13 Wyeth Llc Methods for purification of single domain antigen binding molecules
US20120101262A1 (en) 2009-06-25 2012-04-26 Bristol-Myers Squibb Company Protein purification by caprylic acid (octanoic acid) precipitation
EP3715356B1 (en) 2009-12-18 2023-10-11 CSL Limited Method of purifying polypeptides
EP4492053A3 (en) * 2010-05-25 2025-03-19 F. Hoffmann-La Roche AG Methods of purifying polypeptides
CA2810909A1 (en) * 2010-09-20 2012-03-29 Abbvie Inc. Purification of antibodies using simulated moving bed chromatography
EP2632562B1 (en) * 2010-10-27 2020-08-05 Philip Morris Products S.a.s. Methods for capturing virus like particles from plants using expanded bed chromatography
CN102127164B (zh) 2010-12-20 2013-01-30 武汉禾元生物科技有限公司 一种从水稻种子中提取重组人血清白蛋白的方法
US20140162317A1 (en) 2010-12-21 2014-06-12 Roberto Falkenstein Isoform entriched antibody preparation and method for obtaining it
CN102532254B (zh) * 2010-12-24 2015-06-24 武汉禾元生物科技股份有限公司 一种从水稻种子中分离纯化重组人血清白蛋白的方法
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US9260536B2 (en) 2011-07-22 2016-02-16 Stellan Hjerten Capture of pathogenic and non-pathogenic biopolymers and bioparticles
CN103028376B (zh) * 2011-09-29 2015-05-13 佛山市博新生物科技有限公司 一种用于清除血液毒素的血液净化吸附剂及其制备方法
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
CN104411820B (zh) 2012-06-29 2017-05-03 Emd密理博公司 在蛋白纯化过程中灭活病毒的方法
KR20150043523A (ko) 2012-09-02 2015-04-22 애브비 인코포레이티드 단백질 불균일성의 제어 방법
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
CN103880947B (zh) 2012-12-21 2016-01-13 武汉禾元生物科技股份有限公司 一种分离纯化高纯度重组人血清白蛋白的层析方法
SG11201507230PA (en) 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
KR101569783B1 (ko) 2013-06-05 2015-11-19 한화케미칼 주식회사 항체의 정제 방법
AU2014310480B2 (en) 2013-08-23 2020-03-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Microparticles for cell disruption and/or biomolecule recovery
JP6602765B2 (ja) * 2013-09-05 2019-11-06 ジェネンテック， インコーポレイテッド クロマトグラフィー再使用のための方法
EP3683232A1 (en) 2013-09-13 2020-07-22 F. Hoffmann-La Roche AG Methods and compositions comprising purified recombinant polypeptides
EP3052640A2 (en) 2013-10-04 2016-08-10 AbbVie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
MX384829B (es) * 2014-04-23 2025-03-14 Alexion Pharma Inc Procesamiento de clara de huevo.
CN105296433B (zh) 2014-08-01 2018-02-09 中山康方生物医药有限公司 一种ctla4抗体、其药物组合物及其用途
US10738078B2 (en) 2014-11-03 2020-08-11 Bristol-Myers Squibb Company Use of caprylic acid precipitation for protein purification
ES3006358T3 (en) * 2015-03-13 2025-03-18 Novimmune Sa Methods of purifying bispecific antibodies
CN105777896B (zh) * 2015-03-19 2019-08-16 广东东阳光药业有限公司 一种抗体酸性峰的纯化方法
WO2018035710A1 (en) 2016-08-23 2018-03-01 Akeso Biopharma, Inc. Anti-ctla4 antibodies
CN107413312A (zh) * 2017-07-18 2017-12-01 天津工业大学 一种用于抗体纯化的混合模式蛋白吸附膜及制备方法
MA49962A (fr) * 2017-08-22 2020-07-01 Biogen Ma Inc Procédés de purification d'anticorps présentant des agrégats réduits à poids moléculaire élevé
JP7786876B2 (ja) 2017-12-21 2025-12-16 ジェンザイム・コーポレーション プロテインａクロマトグラフィー中の不純物の除去を促進する方法
CN108239146A (zh) * 2018-03-26 2018-07-03 江苏中新医药有限公司 一种高纯度rhNGF的制备方法
CN109021066A (zh) * 2018-08-23 2018-12-18 上海青平药业有限公司 一种发酵液提取谷胱甘肽的方法
CN110348090B (zh) * 2019-06-28 2021-05-04 浙江大学 基于人工神经网络实现多柱连续流层析设计及分析的方法
KR102170372B1 (ko) 2019-08-13 2020-10-27 주식회사 세이포드 외이도 내 인체 조직에 음향 전달을 위한 사운드 앵커 및 이를 구비한 반이식형 보청기
WO2022018619A1 (en) * 2020-07-20 2022-01-27 Purolite (China) Co., Ltd. Methods for chromatographic protein extraction and purification
CN111991571B (zh) * 2020-08-12 2022-04-05 湖州师范学院 一种柱上低pH病毒灭活的方法
CN112094816B (zh) * 2020-09-23 2021-06-04 绿城农科检测技术有限公司 分泌2-巯基苯并咪唑单克隆抗体的杂交瘤细胞株、单克隆抗体及应用
US20250249437A1 (en) * 2022-04-08 2025-08-07 Csl Behring Ag Methods of sanitizing and/or regenerating a chromatography medium
CN119113574B (zh) * 2024-09-10 2025-04-18 宝利化（南京）制药有限公司 一种熊去氧胆酸制备纯化装置、方法及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7138262B1 (en) * 2000-08-18 2006-11-21 Shire Human Genetic Therapies, Inc. High mannose proteins and methods of making high mannose proteins
US20040018194A1 (en) * 2000-11-28 2004-01-29 Francisco Joseph A. Recombinant anti-CD30 antibodies and uses thereof
CA2451955C (en) * 2001-06-26 2015-09-29 Abgenix, Inc. Antibodies to opgl
DK1501369T3 (en) * 2002-04-26 2015-09-28 Genentech Inc Non-affinity purification of proteins
LT1543038T (lt) * 2002-09-11 2017-07-10 Genentech, Inc. Baltymo gryninimas
ES2285543T3 (es) 2003-11-05 2007-11-16 Ares Trading S.A. Procedimiento para la purificacion de la proteina de union a il-18.
US8129508B2 (en) 2005-04-11 2012-03-06 Medarex, Inc. Protein purification using HCIC and ion exchange chromatography
EP2001898A1 (en) * 2006-03-20 2008-12-17 Medarex Inc. Protein purification

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016512957A (ja) * 2013-02-22 2016-05-12 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ 負帯電粒子を用いるウイルス調製物のクロマトグラフィー精製
US9902942B2 (en) 2013-02-22 2018-02-27 Agency For Science, Technology And Research Chromatographic purification of virus preparations with negatively charged particles
JP2016531304A (ja) * 2013-09-13 2016-10-06 ジェネンテック， インコーポレイテッド 細胞株中の宿主細胞タンパク質及び組換えポリペプチド産物を検出及び定量化するための組成物並びに方法
WO2015041218A1 (ja) 2013-09-17 2015-03-26 株式会社カネカ 新規抗体精製方法及びそれから得られる抗体（Ｎｏｖｅｌ Ａｎｔｉｂｏｄｙ Ｐｕｒｉｆｉｃａｔｉｏｎ Ｍｅｔｈｏｄ ａｎｄ Ａｎｔｉｂｏｄｙ ｏｂｔａｉｎｅｄ ｔｈｅｒｅｆｒｏｍ）、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体（Ｎｏｖｅｌ Ａｎｔｉｂｏｄｙ Ｐｕｒｉｆｉｃａｔｉｏｎ ｍｅｔｈｏｄ ｕｓｉｎｇ Ｃａｔｉｏｎ Ｅｘｃｈａｎｇｅｒ ａｎｄ Ａｎｔｉｂｏｄｙ ｏｂｔａｉｎｅｄ ｔｈｅｒｅｆｒｏｍ）
US10519195B2 (en) 2013-09-17 2019-12-31 Kaneka Corporation Antibody purification method, antibody obtained therefrom, novel antibody purification method using cation exchanger, and antibody obtained therefrom
WO2020067027A1 (ja) * 2018-09-28 2020-04-02 一般財団法人阪大微生物病研究会 Ｖｌｐ発現ｃｈｏ細胞株の構築
JPWO2020067027A1 (ja) * 2018-09-28 2021-08-30 一般財団法人阪大微生物病研究会 Ｖｌｐ発現ｃｈｏ細胞株の構築

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US20140179904A1 (en) 2014-06-26
NZ562949A (en) 2009-05-31
WO2006110277A1 (en) 2006-10-19
EA013504B1 (ru) 2010-06-30
MX2007012499A (es) 2007-12-06
KR20080006601A (ko) 2008-01-16
US8129508B2 (en) 2012-03-06
US8697847B2 (en) 2014-04-15
EA200702210A1 (ru) 2008-04-28
EP1869067A1 (en) 2007-12-26
US20090105465A1 (en) 2009-04-23
AU2006234909A1 (en) 2006-10-19
CN101218247A (zh) 2008-07-09
US20120165511A1 (en) 2012-06-28
CA2604877A1 (en) 2006-10-19

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