JP2008535913A5 - - Google Patents
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- JP2008535913A5 JP2008535913A5 JP2008506476A JP2008506476A JP2008535913A5 JP 2008535913 A5 JP2008535913 A5 JP 2008535913A5 JP 2008506476 A JP2008506476 A JP 2008506476A JP 2008506476 A JP2008506476 A JP 2008506476A JP 2008535913 A5 JP2008535913 A5 JP 2008535913A5
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- JP
- Japan
- Prior art keywords
- range
- target protein
- antibody
- charge induction
- hydrophobic charge
- Prior art date
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- 238000000034 method Methods 0.000 claims 32
- 108090000623 proteins and genes Proteins 0.000 claims 19
- 102000004169 proteins and genes Human genes 0.000 claims 19
- 230000002209 hydrophobic effect Effects 0.000 claims 13
- 230000006698 induction Effects 0.000 claims 13
- 238000005277 cation exchange chromatography Methods 0.000 claims 8
- 238000004587 chromatography analysis Methods 0.000 claims 8
- -1 sulfoisobutyl Chemical group 0.000 claims 5
- 238000011210 chromatographic step Methods 0.000 claims 4
- 239000003446 ligand Substances 0.000 claims 4
- 150000001768 cations Chemical class 0.000 claims 3
- 239000000919 ceramic Substances 0.000 claims 3
- 239000012149 elution buffer Substances 0.000 claims 3
- 239000000203 mixture Substances 0.000 claims 3
- 239000011534 wash buffer Substances 0.000 claims 3
- YHMYGUUIMTVXNW-UHFFFAOYSA-N 1,3-dihydrobenzimidazole-2-thione Chemical compound C1=CC=C2NC(S)=NC2=C1 YHMYGUUIMTVXNW-UHFFFAOYSA-N 0.000 claims 2
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims 2
- 229920000936 Agarose Polymers 0.000 claims 2
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 claims 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 2
- 229920002684 Sepharose Polymers 0.000 claims 2
- YTPLMLYBLZKORZ-UHFFFAOYSA-N Thiophene Chemical compound C=1C=CSC=1 YTPLMLYBLZKORZ-UHFFFAOYSA-N 0.000 claims 2
- 241000700605 Viruses Species 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 2
- 239000000356 contaminant Substances 0.000 claims 2
- 230000014509 gene expression Effects 0.000 claims 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims 2
- 230000002779 inactivation Effects 0.000 claims 2
- IWYDHOAUDWTVEP-UHFFFAOYSA-N mandelic acid Chemical compound OC(=O)C(O)C1=CC=CC=C1 IWYDHOAUDWTVEP-UHFFFAOYSA-N 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 229920001467 poly(styrenesulfonates) Polymers 0.000 claims 2
- 229920005989 resin Polymers 0.000 claims 2
- 239000011347 resin Substances 0.000 claims 2
- 125000000472 sulfonyl group Chemical group *S(*)(=O)=O 0.000 claims 2
- GIANIJCPTPUNBA-QMMMGPOBSA-N (2s)-3-(4-hydroxyphenyl)-2-nitramidopropanoic acid Chemical compound [O-][N+](=O)N[C@H](C(=O)O)CC1=CC=C(O)C=C1 GIANIJCPTPUNBA-QMMMGPOBSA-N 0.000 claims 1
- FQXRXTUXSODUFZ-UHFFFAOYSA-N 1h-imidazol-2-ylmethanethiol Chemical compound SCC1=NC=CN1 FQXRXTUXSODUFZ-UHFFFAOYSA-N 0.000 claims 1
- FHTDDANQIMVWKZ-UHFFFAOYSA-N 1h-pyridine-4-thione Chemical compound SC1=CC=NC=C1 FHTDDANQIMVWKZ-UHFFFAOYSA-N 0.000 claims 1
- WOXFMYVTSLAQMO-UHFFFAOYSA-N 2-Pyridinemethanamine Chemical compound NCC1=CC=CC=N1 WOXFMYVTSLAQMO-UHFFFAOYSA-N 0.000 claims 1
- UTNSTTZHNMPBEE-UHFFFAOYSA-N 2-chloro-2-hydroxy-2-phenylacetic acid Chemical compound OC(=O)C(O)(Cl)C1=CC=CC=C1 UTNSTTZHNMPBEE-UHFFFAOYSA-N 0.000 claims 1
- WWWFHFGUOIQNJC-UHFFFAOYSA-N 2-hydroxy-3-nitrobenzoic acid Chemical compound OC(=O)C1=CC=CC([N+]([O-])=O)=C1O WWWFHFGUOIQNJC-UHFFFAOYSA-N 0.000 claims 1
- USVXDODAPOBXCF-UHFFFAOYSA-N 2-methyl-1h-benzimidazol-4-amine Chemical compound C1=CC=C2NC(C)=NC2=C1N USVXDODAPOBXCF-UHFFFAOYSA-N 0.000 claims 1
- VBAOEVKQBLGWTH-UHFFFAOYSA-N 2-pyridin-4-ylethanethiol Chemical compound SCCC1=CC=NC=C1 VBAOEVKQBLGWTH-UHFFFAOYSA-N 0.000 claims 1
- YDOVRFJDZXIYMW-UHFFFAOYSA-N 3,4-dichloro-2-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(Cl)C(Cl)=C1O YDOVRFJDZXIYMW-UHFFFAOYSA-N 0.000 claims 1
- HQNOODJDSFSURF-UHFFFAOYSA-N 3-(1h-imidazol-2-yl)propan-1-amine Chemical compound NCCCC1=NC=CN1 HQNOODJDSFSURF-UHFFFAOYSA-N 0.000 claims 1
- UIKUBYKUYUSRSM-UHFFFAOYSA-N 3-morpholinopropylamine Chemical compound NCCCN1CCOCC1 UIKUBYKUYUSRSM-UHFFFAOYSA-N 0.000 claims 1
- NJGSDIDEYCTWLX-UHFFFAOYSA-N 4-[2-(dibromoamino)ethyl]phenol Chemical compound OC1=CC=C(CCN(Br)Br)C=C1 NJGSDIDEYCTWLX-UHFFFAOYSA-N 0.000 claims 1
- SLXKOJJOQWFEFD-UHFFFAOYSA-N 6-aminohexanoic acid Chemical compound NCCCCCC(O)=O SLXKOJJOQWFEFD-UHFFFAOYSA-N 0.000 claims 1
- 108010024636 Glutathione Proteins 0.000 claims 1
- 101100174574 Mus musculus Pikfyve gene Proteins 0.000 claims 1
- DZGWFCGJZKJUFP-UHFFFAOYSA-N Tyramine Natural products NCCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-N 0.000 claims 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 claims 1
- 238000001042 affinity chromatography Methods 0.000 claims 1
- 125000001931 aliphatic group Chemical group 0.000 claims 1
- 229960002684 aminocaproic acid Drugs 0.000 claims 1
- 125000003118 aryl group Chemical group 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- QAOWNCQODCNURD-UHFFFAOYSA-M bisulphate group Chemical group S([O-])(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-M 0.000 claims 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims 1
- 239000001768 carboxy methyl cellulose Substances 0.000 claims 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims 1
- 150000001732 carboxylic acid derivatives Chemical class 0.000 claims 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims 1
- 229940105329 carboxymethylcellulose Drugs 0.000 claims 1
- QYJXLKYOBNZROU-UHFFFAOYSA-N carboxysulfonylformic acid Chemical compound OC(=O)S(=O)(=O)C(O)=O QYJXLKYOBNZROU-UHFFFAOYSA-N 0.000 claims 1
- 238000005341 cation exchange Methods 0.000 claims 1
- 239000003729 cation exchange resin Substances 0.000 claims 1
- 229940023913 cation exchange resins Drugs 0.000 claims 1
- 238000004113 cell culture Methods 0.000 claims 1
- 229920001429 chelating resin Polymers 0.000 claims 1
- 238000009295 crossflow filtration Methods 0.000 claims 1
- 239000012228 culture supernatant Substances 0.000 claims 1
- 229960003180 glutathione Drugs 0.000 claims 1
- 125000000623 heterocyclic group Chemical group 0.000 claims 1
- 229960001340 histamine Drugs 0.000 claims 1
- 125000002883 imidazolyl group Chemical group 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- QOHMWDJIBGVPIF-UHFFFAOYSA-N n',n'-diethylpropane-1,3-diamine Chemical compound CCN(CC)CCCN QOHMWDJIBGVPIF-UHFFFAOYSA-N 0.000 claims 1
- 239000008194 pharmaceutical composition Substances 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- HDOUGSFASVGDCS-UHFFFAOYSA-N pyridin-3-ylmethanamine Chemical compound NCC1=CC=CN=C1 HDOUGSFASVGDCS-UHFFFAOYSA-N 0.000 claims 1
- TXQWFIVRZNOPCK-UHFFFAOYSA-N pyridin-4-ylmethanamine Chemical compound NCC1=CC=NC=C1 TXQWFIVRZNOPCK-UHFFFAOYSA-N 0.000 claims 1
- WHMDPDGBKYUEMW-UHFFFAOYSA-N pyridine-2-thiol Chemical compound SC1=CC=CC=N1 WHMDPDGBKYUEMW-UHFFFAOYSA-N 0.000 claims 1
- 230000000630 rising effect Effects 0.000 claims 1
- 239000001488 sodium phosphate Substances 0.000 claims 1
- 229910000162 sodium phosphate Inorganic materials 0.000 claims 1
- RVEZZJVBDQCTEF-UHFFFAOYSA-N sulfenic acid Chemical compound SO RVEZZJVBDQCTEF-UHFFFAOYSA-N 0.000 claims 1
- AGGIJOLULBJGTQ-UHFFFAOYSA-N sulfoacetic acid Chemical compound OC(=O)CS(O)(=O)=O AGGIJOLULBJGTQ-UHFFFAOYSA-N 0.000 claims 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 claims 1
- 229910052717 sulfur Inorganic materials 0.000 claims 1
- 125000004434 sulfur atom Chemical group 0.000 claims 1
- 125000003396 thiol group Chemical class [H]S* 0.000 claims 1
- 229930192474 thiophene Natural products 0.000 claims 1
- 229940015849 thiophene Drugs 0.000 claims 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims 1
- 229960003732 tyramine Drugs 0.000 claims 1
- DZGWFCGJZKJUFP-UHFFFAOYSA-O tyraminium Chemical compound [NH3+]CCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-O 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US67045705P | 2005-04-11 | 2005-04-11 | |
| PCT/US2006/010412 WO2006110277A1 (en) | 2005-04-11 | 2006-03-21 | Protein purification using hcic amd ion exchange chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008535913A JP2008535913A (ja) | 2008-09-04 |
| JP2008535913A5 true JP2008535913A5 (enExample) | 2009-04-23 |
Family
ID=36764783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008506476A Withdrawn JP2008535913A (ja) | 2005-04-11 | 2006-03-21 | タンパク質精製方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US8129508B2 (enExample) |
| EP (1) | EP1869067A1 (enExample) |
| JP (1) | JP2008535913A (enExample) |
| KR (1) | KR20080006601A (enExample) |
| CN (1) | CN101218247A (enExample) |
| AU (1) | AU2006234909A1 (enExample) |
| CA (1) | CA2604877A1 (enExample) |
| EA (1) | EA013504B1 (enExample) |
| MX (1) | MX2007012499A (enExample) |
| NZ (1) | NZ562949A (enExample) |
| WO (1) | WO2006110277A1 (enExample) |
Families Citing this family (66)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8129508B2 (en) | 2005-04-11 | 2012-03-06 | Medarex, Inc. | Protein purification using HCIC and ion exchange chromatography |
| EP2738179A1 (en) | 2006-04-05 | 2014-06-04 | AbbVie Biotechnology Ltd | Antibody purification |
| US8513393B2 (en) * | 2006-08-28 | 2013-08-20 | Ares Trading S.A. | Process for the purification of Fc-containing proteins |
| FR2910786B1 (fr) * | 2006-12-29 | 2017-08-11 | Laboratoire Francais Du Fractionnement Et Des Biotechnologies (Lfb) | "procede d'extraction d'une proteine presente dans du lait" |
| US20100062512A1 (en) * | 2007-01-04 | 2010-03-11 | Crucell Holland B.V. | Purification of factor xi |
| KR100955470B1 (ko) | 2008-01-09 | 2010-04-30 | 대한민국(관리부서 질병관리본부장) | 탄저 방어 항원의 제조 방법 |
| CN102177237B (zh) * | 2008-09-12 | 2013-10-30 | 金沃特公司 | 用于贮存和稳定生物分子的基质和介质 |
| TW201024318A (en) | 2008-10-20 | 2010-07-01 | Abbott Lab | Isolation and purification of antibodies using protein A affinity chromatography |
| SG195577A1 (en) | 2008-10-20 | 2013-12-30 | Abbott Lab | Viral inactivation during purification of antibodies |
| EP2346901A1 (en) * | 2008-10-20 | 2011-07-27 | Abbott Laboratories | Antibodies that bind to il-18 and methods of purifying the same |
| CA2739352C (en) * | 2008-10-29 | 2021-07-13 | Wyeth Llc | Methods for purification of single domain antigen binding molecules |
| US20120101262A1 (en) | 2009-06-25 | 2012-04-26 | Bristol-Myers Squibb Company | Protein purification by caprylic acid (octanoic acid) precipitation |
| EP3715356B1 (en) | 2009-12-18 | 2023-10-11 | CSL Limited | Method of purifying polypeptides |
| EP4492053A3 (en) * | 2010-05-25 | 2025-03-19 | F. Hoffmann-La Roche AG | Methods of purifying polypeptides |
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Family Cites Families (8)
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|---|---|---|---|---|
| US7138262B1 (en) * | 2000-08-18 | 2006-11-21 | Shire Human Genetic Therapies, Inc. | High mannose proteins and methods of making high mannose proteins |
| US20040018194A1 (en) * | 2000-11-28 | 2004-01-29 | Francisco Joseph A. | Recombinant anti-CD30 antibodies and uses thereof |
| CA2451955C (en) * | 2001-06-26 | 2015-09-29 | Abgenix, Inc. | Antibodies to opgl |
| DK1501369T3 (en) * | 2002-04-26 | 2015-09-28 | Genentech Inc | Non-affinity purification of proteins |
| LT1543038T (lt) * | 2002-09-11 | 2017-07-10 | Genentech, Inc. | Baltymo gryninimas |
| ES2285543T3 (es) | 2003-11-05 | 2007-11-16 | Ares Trading S.A. | Procedimiento para la purificacion de la proteina de union a il-18. |
| US8129508B2 (en) | 2005-04-11 | 2012-03-06 | Medarex, Inc. | Protein purification using HCIC and ion exchange chromatography |
| EP2001898A1 (en) * | 2006-03-20 | 2008-12-17 | Medarex Inc. | Protein purification |
-
2006
- 2006-03-21 US US11/918,155 patent/US8129508B2/en active Active
- 2006-03-21 EP EP06748555A patent/EP1869067A1/en not_active Withdrawn
- 2006-03-21 AU AU2006234909A patent/AU2006234909A1/en not_active Abandoned
- 2006-03-21 CN CNA2006800166104A patent/CN101218247A/zh active Pending
- 2006-03-21 MX MX2007012499A patent/MX2007012499A/es not_active Application Discontinuation
- 2006-03-21 CA CA002604877A patent/CA2604877A1/en not_active Abandoned
- 2006-03-21 KR KR1020077026129A patent/KR20080006601A/ko not_active Withdrawn
- 2006-03-21 WO PCT/US2006/010412 patent/WO2006110277A1/en not_active Ceased
- 2006-03-21 JP JP2008506476A patent/JP2008535913A/ja not_active Withdrawn
- 2006-03-21 EA EA200702210A patent/EA013504B1/ru not_active IP Right Cessation
- 2006-03-21 NZ NZ562949A patent/NZ562949A/en not_active IP Right Cessation
-
2012
- 2012-03-02 US US13/410,740 patent/US8697847B2/en not_active Expired - Lifetime
-
2014
- 2014-02-28 US US14/193,731 patent/US20140179904A1/en not_active Abandoned
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