CN101129174A - Process for preparing organic selenium pine pollen - Google Patents

Process for preparing organic selenium pine pollen Download PDF

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Publication number
CN101129174A
CN101129174A CNA2007101318539A CN200710131853A CN101129174A CN 101129174 A CN101129174 A CN 101129174A CN A2007101318539 A CNA2007101318539 A CN A2007101318539A CN 200710131853 A CN200710131853 A CN 200710131853A CN 101129174 A CN101129174 A CN 101129174A
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organic selenium
selenium
biological fluids
pine pollen
agent
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CN101129174B (en
Inventor
王学林
钱进
王春山
李玉峰
钱海燕
凌中鑫
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ANHUI PROVINCE HUADING BIOTECHNOLOGY Co Ltd
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ANHUI PROVINCE HUADING BIOTECHNOLOGY Co Ltd
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Abstract

The invention relates to a making method of organic selenium pine pollen belonging in biotechnological field with the purpose of providing the making method that can amplify utility of the pine pollen and improve nutrition and medicinal value of the organic selenium pine pollen, which is characterized by the following: converting inorganic selenium to be the organic selenium; using straw of agronomic crop as base material to add absorbent and water-loss agent to produce the organic selenium agent; getting the organic selenium pine pollen with 10-50mg Se/kg selenium through reasonable cultivating and biotransformation. In comparison with the general, the organic selenium pine pollen improves the nutrition and medicinal values greatly with selenium that is 100-500 times as high as the general, which can be adopted in fields of food, health products, drug and medical treatment extensively because of the selenium being organic state with more stable and secure. In addition, the technique can be controlled to operate easily and the raw material can be purchasing easily with the low production cost and high added value of the product.

Description

The production method of organic selenium pine pollen
Technical field:
The present invention relates to a kind of is carrier with the pollen pini, obtains the production method of organic selenium pine pollen by bio-transformation.
Background technology:
Selenium is biological essential trace element.Selenium has the effect that strengthens immunity, anti-ageing and anti-curing oncoma in vivo.Scarce selenium causes body's immunity and oxidation resistance to reduce, and with being associated of multiple disease, replenish an amount of selenium and can improve immunity of organisms, prevent multiple disease, therefore be subjected to extensive attention.Propose in 1988 according to Chinese Soclety of Nutrition, adult's meals selenium quantity delivered every day (RDA) is 50ug.And in mending the anticancer grade of selenium intervention experiment, every day, the Selenium supplement agent amount was 200ug, and used selenium is Na 2Seo 3And selenium yeast.Selenium is in vivo mainly by selenium enzyme and selenoprotein performance biological function.
Be used to mend selenium compound at present and be mainly inorganic selenium such as Na 2Seo 3Deng.Inorganic selenium is bigger than organic selenium toxicity, and the working condition harshness of organic selenium, cost an arm and a leg, the method that obtains organic selenium at present is generally utilizes self transforming of some plants to selenium high-adaptability and high conversion, higher fungus, but lacks abundant nutrient source.
Pollen pini is from ancient times just as the food and the medicine of nourishing and fit keeping function, beauty treatment, diseases prevention.Studies confirm that the pollen pini nutritive value is higher, be rich in essential amino acid, have and improve immunity, protection liver and function such as antitumor, is the fabulous source that replenishes essential amino acid.
Though China's pollen pini resource is abundant, since not enough to the dynamics of its development and use, cause sizable a part of pollen pini to run off in vain, waste.
Summary of the invention
The object of the present invention is to provide a kind of dynamics that can strengthen, and can further promote the production method of the organic selenium pine pollen of the nutritive value of pollen pini and medical value pollen pini development and use.
Its technical scheme is: a kind of production method of organic selenium pine pollen is characterized in that:
A) preparation of EM bacterial strain biological fluids: in M bacterial strain stoste, add Hong Tang and water, the weight proportion of M bacterial strain stoste and Hong Tang and water is: 1: 0.5: 10; Under 20-25 ℃ of condition, cultivated 7-15 days, get EM bacterial strain biological fluids;
B) preparation of organic selenium biological fluids: selenate is added in the EM bacterial strain biological fluids in batches, and the organic selenium content in adding biological fluids to is till 0.12% o'clock; After fermentation, promptly get organic selenium biological fluids;
C) preparation of organic selenium agent: add corncob and water-absorbing water-retaining agent in above-mentioned organic selenium biological fluids, the weight proportion of organic selenium biological fluids and corncob and high-efficiency water-absorbing water-loss reducer is 1: 0.3-0.4: 0.1-0.2 makes organic selenium agent through stirring;
D) using of organic selenium agent: select the masson pine of the 15-25 age of tree, outside the circumference range of distance trunk 50-100cm, the base that burrows is executed above-mentioned pollen pini selenium-rich agent, then, covers the hole with film, in case the rainwater swash of wave;
E) acquisition of organic selenium pine pollen: bloom the time masson pine, through pluck, ted, after dry, the impurity elimination, the content that can obtain selenium is the organic selenium pine pollen of 10-50mg Se/kg.
Above-mentioned organic selenium pine pollen can be prepared into pulvis, tablet, injection, glue and assist agent and oral liquor and other types of formulations behind broken wall, to satisfy the needs that various age brackets and various health status crowd take.
Its technique effect: the production method of organic selenium pine pollen of the present invention is converted into organic selenium that human body absorbs easily by microorganism with inorganic selenium; Make matrix with the straw of crops again, add the high-efficiency water-absorbing water-loss reducer, make organic selenium agent; At last, by rational cultivation, bio-transformation, can obtain the organic selenium pine pollen that Se content is 10-50mg Se/kg.Compare with common pollen pini, the content of its selenium is than the high 100-500 of 0.1mg Se/kg times of common pollen pini, thereby promoted the nutritive value and the medical value of pollen pini greatly, made it obtain broader applications in fields such as food, health products, medicine, medical supplies; And this selenium is a kind of organic type, comparatively stable, safety.In addition, technological process of the present invention is easy to grasp, and is convenient to operation; Its required raw material is easy to buying, and is with low cost, the value-added content of product height; It is for the deep development utilization of forestry products, rural area industrialization structural adjustment and all have very important realistic meaning for the income that improves the forest zone peasant.
The specific embodiment
The preparation of EM biological fluids: get M bacterial strain stoste 5kg, drop into red pool 2.5kg, water 50kg, under 20-25 ℃ of condition, cultivated 7-15 days, get EM bacterial strain biological fluids.
The preparation of organic selenium biological fluids: with concentration is that 0.15% selenate adds in the above-mentioned EM bacterial strain biological fluids in batches, and the amount that selenate drops into first is 25ug/ml; After, every during the fermentation interval 6-12 hour, the amount of adding 50ug/ml, 75ug/ml, 100ug/ml... successively, the organic selenium content in adding biological fluids to is till 0.12% o'clock; Be that fermenting promptly gets organic selenium biological fluids under 20-25 ℃ the condition after 7 days in temperature.
The preparation of organic selenium agent: the graininess corncob cellulose and the water-absorbing water-retaining agent that add in above-mentioned organic selenium biological fluids after pulverizing (are " good fortune people-brand " water-absorbing water-retaining agent that folk music good fortune people Fine Chemical Co., Ltd produces, it is 00108404.6 that its raw material components and proportioning thereof are asked for an interview the attached annex 1-patent No., name is called the patent of invention specification of " multifunctional super water-absorbing water-retaining agent and preparation method "), the weight proportion of organic selenium biological fluids and corncob cellulose and water-absorbing water-retaining agent is 1: 0.3-0.4: 0.1-0.2, organic selenium biological fluids and corncob cellulose and water-absorbing water-retaining agent make organic selenium agent through stirring.
Using of organic selenium agent: in autumn, the masson pine of the selection 15-25 age of tree is outside the circumference range of distance trunk 50-100cm, adopt the radial base that burrows of 6 dihedrals to execute, punching degree of depth 25-50cm, every hole applies 50-250 and restrains organic selenium agent, then, cover the hole, in case the rainwater swash of wave with film.
The acquisition of organic selenium pine pollen: organic selenium agent is used in before and after back next year Ching Ming Festival 20 days, and promptly masson pine blooms the time, through plucks, ted, after the drying, impurity elimination, the content that can obtain selenium is the organic selenium pine pollen of 10mg Se/kg-50mg Se/kg.

Claims (2)

1. the production method of an organic selenium pine pollen is characterized in that:
A) preparation of EM bacterial strain biological fluids: in M bacterial strain stoste, add Hong Tang and water, the weight proportion of M bacterial strain stoste and Hong Tang and water is: 1: 0.5: 10; Under 20-25 ℃ of condition, cultivated 7-15 days, get EM bacterial strain biological fluids;
B) preparation of organic selenium biological fluids: selenate is added in the EM bacterial strain biological fluids in batches, and the organic selenium content in adding biological fluids to is till 0.12% o'clock; After fermentation, promptly get organic selenium biological fluids;
C) preparation of organic selenium agent: add corncob and water-absorbing water-retaining agent in above-mentioned organic selenium biological fluids, the weight proportion of organic selenium biological fluids and corncob and high-efficiency water-absorbing water-loss reducer is 1: 0.3-0.4: 0.1-0.2 makes organic selenium agent through stirring;
D) using of organic selenium agent: select the masson pine of the 15-25 age of tree, outside the circumference range of distance trunk 50-100cm, the base that burrows is executed above-mentioned organic selenium agent, then, covers the hole with film, in case the rainwater swash of wave;
E) acquisition of organic selenium pine pollen: bloom the time masson pine, through pluck, ted, after dry, the impurity elimination, the content that can obtain selenium is the organic selenium pine pollen of 10-50mg Se/kg.
2. the production method of a kind of organic selenium pine pollen according to claim 1 is characterized in that: described organic selenium pine pollen can be prepared into pulvis, tablet, injection, glue and assist agent or oral liquid formulation behind broken wall.
CN2007101318539A 2007-09-06 2007-09-06 Process for preparing organic selenium pine pollen Expired - Fee Related CN101129174B (en)

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102144632A (en) * 2011-01-30 2011-08-10 广西壮族自治区林业科学研究院 Method for storing and unfreezing pinus massoniana pollen
CN102370127A (en) * 2010-08-25 2012-03-14 杨声谋 Method for industrial production of fresh and alive pollen by centralized growing of male flowers of kiwi fruit
CN102577726A (en) * 2012-01-18 2012-07-18 天津天保园林环卫发展有限公司 Minimally invasive fertilization method
CN103460874A (en) * 2013-08-29 2013-12-25 王学林 Production method of traditional Chinese medicine pollen pini for reducing blood glucose
CN104920053A (en) * 2015-07-02 2015-09-23 安徽恩赐方生物科技有限公司 Method for planting microelement-enriched cistanche
CN104938285A (en) * 2015-07-02 2015-09-30 安徽恩赐方生物科技有限公司 Magnesium-rich eucommia plantation method
CN105052533A (en) * 2015-07-02 2015-11-18 安徽恩赐方生物科技有限公司 Trace element enriching Poria cocos plantation method
CN105309855A (en) * 2014-07-18 2016-02-10 武汉蜂之宝蜂业有限公司 Processing method for selenium-rich pollen sheet
CN106233909A (en) * 2016-08-15 2016-12-21 安徽省华鼎生物科技有限公司 A kind of zinc-rich Pollen Pini and production method thereof
CN106262831A (en) * 2016-08-15 2017-01-04 安徽省华鼎生物科技有限公司 A kind of chromium-rich selenium pine pollen and production method thereof
CN106262379A (en) * 2016-08-15 2017-01-04 安徽省华鼎生物科技有限公司 A kind of rich calcium Pollen Pini and production method thereof
CN112033134A (en) * 2020-09-10 2020-12-04 中国农业科学院蜜蜂研究所 Instant drying device for fresh pollen and operation method thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1114672C (en) * 2000-04-29 2003-07-16 兰州大学 Multifunctional super water-absorbing water-retaining agent and its preparation method
CN1237029C (en) * 2003-12-31 2006-01-18 南京新世纪园艺研究所 Selenium enriched liquid fertilizer and public hazard less production method of selenium enriched aqgricultural product thereof
CN1803012A (en) * 2005-01-10 2006-07-19 邹静中 Preparation method of selenium enriched health product containing composite active probiotic formulation
CN100493520C (en) * 2005-05-11 2009-06-03 南京师范大学 Selenium-rich lactobacillus preparation and its production process and application

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102370127A (en) * 2010-08-25 2012-03-14 杨声谋 Method for industrial production of fresh and alive pollen by centralized growing of male flowers of kiwi fruit
CN102370127B (en) * 2010-08-25 2013-09-11 杨声谋 Method for industrial production of fresh and alive pollen by centralized growing of male flowers of kiwi fruit
CN102144632A (en) * 2011-01-30 2011-08-10 广西壮族自治区林业科学研究院 Method for storing and unfreezing pinus massoniana pollen
CN102144632B (en) * 2011-01-30 2012-11-21 广西壮族自治区林业科学研究院 Method for storing and unfreezing pinus massoniana pollen
CN102577726A (en) * 2012-01-18 2012-07-18 天津天保园林环卫发展有限公司 Minimally invasive fertilization method
CN103460874A (en) * 2013-08-29 2013-12-25 王学林 Production method of traditional Chinese medicine pollen pini for reducing blood glucose
CN105309855A (en) * 2014-07-18 2016-02-10 武汉蜂之宝蜂业有限公司 Processing method for selenium-rich pollen sheet
CN105052533A (en) * 2015-07-02 2015-11-18 安徽恩赐方生物科技有限公司 Trace element enriching Poria cocos plantation method
CN104938285A (en) * 2015-07-02 2015-09-30 安徽恩赐方生物科技有限公司 Magnesium-rich eucommia plantation method
CN104920053A (en) * 2015-07-02 2015-09-23 安徽恩赐方生物科技有限公司 Method for planting microelement-enriched cistanche
CN105052533B (en) * 2015-07-02 2018-06-22 芜湖砻坊生产力促进中心有限公司 A kind of implantation methods of the Poria cocos of Rich in Trace Element
CN106233909A (en) * 2016-08-15 2016-12-21 安徽省华鼎生物科技有限公司 A kind of zinc-rich Pollen Pini and production method thereof
CN106262831A (en) * 2016-08-15 2017-01-04 安徽省华鼎生物科技有限公司 A kind of chromium-rich selenium pine pollen and production method thereof
CN106262379A (en) * 2016-08-15 2017-01-04 安徽省华鼎生物科技有限公司 A kind of rich calcium Pollen Pini and production method thereof
CN112033134A (en) * 2020-09-10 2020-12-04 中国农业科学院蜜蜂研究所 Instant drying device for fresh pollen and operation method thereof
CN112033134B (en) * 2020-09-10 2022-02-22 中国农业科学院蜜蜂研究所 Instant drying device for fresh pollen and operation method thereof

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