CN101760492A

CN101760492A - Method for extracting algae glycoproteins

Info

Publication number: CN101760492A
Application number: CN200810238767A
Authority: CN
Inventors: 徐宝贞; 程潜
Original assignee: Individual
Current assignee: Individual
Priority date: 2008-12-24
Filing date: 2008-12-24
Publication date: 2010-06-30
Anticipated expiration: 2028-12-24
Also published as: CN101760492B

Abstract

The invention discloses a method for extracting algae glycoproteins. The method for extracting the algae glycoproteins comprises the following step: mixing a certain amount of the mixture of algae, blue-green algae or the mixture of alga powder and water with the water solution with specific fungi. The algae glycoproteins extracted by the method have the functions of preventing and treating the respiratory diseases, and also have functions on broad-spectrum antiviral, anti-inflammatory, anticoagulin, bacteriostasis, refrigeration and immune adjustment.

Description

Extract the method for algae glycoproteins

Technical field

The invention belongs to the method for biological extraction algae glycoproteins, a kind of specifically aqueous solution with microorganism extracts the method for algae glycoproteins from blue-green algae, blue-green algae.

Background technology

Algae glycoproteins is an important active substances, because of it is to have very big active polypeptide glycan, can stop the hemagglutinin in the virus and the adhesion of human body cell, and virus is disintegrated, and can significantly improve the immunologic function of human body, has pharmaceutical use and nutritive value.

Studies show that, algae glycoproteins has prevention, treatment respiratory system disease, and broad-spectrum antiviral, anti-inflammatory, anticoagulant, antibacterial, analgesic, immunoloregulation function, eliminate the damage of chemotherapy to hemopoietic function, improve lymphocyte activity, actively the oxyradical in the scavenger cell promotes wound healing.The effectively growth of anticancer of experimentation on animals proof.Past to blue-green algae, blue-green algae broken wall be adopt that machinery is smashed to pieces, the way of multigelation, chemical treatment and ultrasonication, treat from blue-green algae, blue-green algae, to separate out albumen again behind the broken wall, but these methods proteic biological activity of havoc all.At present, the utilization biological process is also arranged, but the utilization biological process can only extract Phycocyanins, C-from blue-green algae, blue-green algae, this utilization biological process extracts Phycocyanins, C-from blue-green algae, blue-green algae method is to be 2005100591888 by the patent No., and name is called that the patent of " a kind of method of extracting Phycocyanins, C-" provides.He mixes with the nutrient solution of blue-green algae, blue-green algae with the nutrient solution that colludes Klebsiella pneumonia, day dimension enterobacteria or acid-producing Klebsiella bacterium, Phycocyanins, C-in blue-green algae, the blue-green algae is discharged in the nutrient solution, further use the ordinary method purifying again, to obtain the method for highly purified Phycocyanins, C-.Though this method is simple and easy to do, cost is low, can only obtain Phycocyanins, C-with this method, and the Phycocyanins, C-of gained is only as the natural pigment of food, senior eye shadow, lipstick, and few of pharmaceutical use, the effect that does not have broad-spectrum antiviral, anti-inflammatory.And this method also is in laboratory stage, and can not be applied to large-scale industrial production.

Summary of the invention

Phycocyanins, C-that technology is in the past extracted from blue-green algae, blue-green algae and polysaccharide all are single polysaccharide and albumen, and both are mixture, rather than binding substances, and its function singleness can only be made food and makeup.Has only minimum pharmacological action.The invention provides and a kind ofly make blue-green algae, blue-green algae or its algae powder natural enzymolysis made from microbial solution, the algae glycoproteins that will have broad-spectrum antiviral, prevention, treatment respiratory system disease is discharged in the fungi solution, use the ordinary method purifying, obtain the method for high purity algae glycoproteins.

The method of this extraction algae glycoproteins comprises the mixture of getting a certain amount of blue-green algae, blue-green algae or algae powder and water, with the aqueous solution of specific fungi.

In this process, spirulina in the blue algae or algae pruinescence rapid enzymolysis

This method further can obtain the high purity algae glycoproteins with the ordinary method purifying.

Wherein a kind of aqueous solution in the aqueous solution of specific fungi is the cellulase aqueous solution in one embodiment of the invention.

The aqueous solution of specific fungi mixes the back gained with blue-green algae, blue-green algae or algae powder algae glycoproteins is blue algae glycoproteins, and the molecular weight of blue algae glycoproteins is 300Da-180 ten thousand Da.

The invention has the beneficial effects as follows that algae glycoproteins has the function of prevention, treatment respiratory system disease, and broad-spectrum antiviral, anti-inflammatory, anticoagulant are antibacterial, analgesic, the effect of immunoloregulation function.And this method provided by the invention is compared with traditional method for extracting proteins from spirulina, it is low to have cost, speed is fast, simple to operate, the algae glycoproteins purity height that extracts, and the active problem of destruction algae glycoproteins that does not have mechanical breaking-wall method, multigelation, chemical treatment, ultrasonication etc. to be caused.Simplify extraction step, be fit to very much industrialization extraction algae glycoproteins from blue-green algae, blue-green algae.

Embodiment

Embodiment 1

1. the breed of spirulina is selected for use

Choose spirulina algae kind, place 27-34 ℃ environment to cultivate the algae kind, the algae kind after the cultivation is put into the pond of open-air sealing printing opacity and is cultured, and fully obtains the irradiation of sunlight, in culturing water, add to contain the nutritive medium of compatibilities such as glucose, amino acid, VITAMIN, obtain spirulina raw material.

2. the preparation of microbial solution

Choose the cellulase in the fungi, under normal temperature condition, put it in the water, make the aqueous solution, with the above-mentioned aqueous solution to pour in the container that fills spirulina by 0.5: 100 volume ratio with spirulina, after stirring, after lucifuge leaves standstill 2 hours, take out supernatant liquor, adopt ordinary method to get blue algae glycoproteins.

Embodiment 2

Carry out the extraction of algae glycoproteins with the method identical with embodiment 1, different is to have changed blue-green algae, blue-green algae into the algae powder, getting the water that the algae powder adds 2 times of amounts of its volume stirs evenly, add the aqueous solution of making again, be poured into leave standstill 1 hour in the algae water mixture after, add 6 times of water gagings again, get blue algae glycoproteins with ordinary method.

Reference: a kind of method of extracting Phycocyanins, C-

Claims

1. a method of extracting algae glycoproteins is characterized in that getting the mixture of a certain amount of blue-green algae, blue-green algae or algae powder and water and the aqueous solution of specific fungi.

2. the method for extraction algae glycoproteins according to claim 1 is characterized in that fungi is a cellulase.

3. the method for extraction algae glycoproteins according to claim 1, the aqueous solution that it is characterized in that fungi are the aqueous solution of cellulase.

4. according to the method for claim 1,2,3 described any extraction algae glycoproteins, it is characterized in that, pour per unit algae powder into and add in the mixture that stirs behind 2 times of water gagings with the solution of 0.5 times of water gaging of used algae powder with cellulase mixing gained.

5. the method for extraction algae glycoproteins according to claim 1 is characterized in that algae glycoproteins is blue.