CN109393489A - Selenium enriched Spirulina powder and preparation method thereof - Google Patents
Selenium enriched Spirulina powder and preparation method thereof Download PDFInfo
- Publication number
- CN109393489A CN109393489A CN201811336769.5A CN201811336769A CN109393489A CN 109393489 A CN109393489 A CN 109393489A CN 201811336769 A CN201811336769 A CN 201811336769A CN 109393489 A CN109393489 A CN 109393489A
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- spirulina
- raw material
- sophora bud
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/14—Yeasts or derivatives thereof
- A23L33/145—Extracts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Inorganic Chemistry (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to a kind of selenium enriched Spirulina powder and preparation method thereof, form as follows: 60-80 parts of spirulina extract, 20-30 parts of buckwheat, 10-15 parts of wolfberry fruit extract, 2-6 parts of sophora bud standard sclerutin, 6-10 parts of Se-enriched yeast extract；The extracting method of the sophora bud standard sclerutin is as follows: S1. raw material smashes: sophora bud rutin raw material drying to moisture content being lower than 8%, is crushed, 60-80 mesh is crossed；S2. aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: hexamethylphosphoramide 15-25%, potassium carbonate 5-8%, ammonium sulfate 3-6%, surplus are water；S3. it extracts: the smashed raw material powder of step S1 is mixed at 40-60 DEG C with the prepared extracting solution of step S2, stir 0.5-1h, stand 30-45min, layering；S4. it separates: taking upper organic phase to be concentrated, precipitate crystal as sophora bud standard sclerutin.Spirulina and sophora bud rutin are carried out deep processing by the present invention, and spirulina enzymolysis extract and sophora bud standard sclerutin are made respectively, then compounds with other components, and nutritive value is high, have great healthy nutritive value.
Description
Technical field
The present invention relates to selenium enriched Spirulina powder and preparation method thereof, belong to nutrient and healthcare products processing technique field.
Background technique
Spirulina is a kind of low photosynthetic autotrophs biology, the filamentous that they are made of unicellular or many cells, in thin
Loose or closely well-regulated spiral rotation shape bending, so gain the name.Spirulina is rich in multiple proteins, multivitamin, mineral
Matter and various bioactivators, it is considered to be the ideal food of human future.Spirulina contain multiple biological activities at
Point: wherein beta carotene, chlorophyll, lutein, polysaccharides and phycocyanin etc. have many unique biochemical properties and life
Manage function.
Beta carotene is the important sources of vitamin A, has anti-oxidant, antitumor, raising immunity of organisms isoreactivity.
Lutein has the function of anti-oxidant and protection retina, referred to as " plant gold ".Phycocyanin is that one kind is prevalent in
Biliproteins in Cells of Blue-green Algae can efficiently capture luminous energy, be a kind of special natural pigment albumen, be in dark blue powder
Shape has water solubility.Phycocyanin can be used as natural pigment additive applied to foods and cosmetics.
Flavone compound is a kind of important natural organic-compound, is that plant generates during long-term natural selection
A kind of secondary metabolite, it is widely present in higher plant and filiciform, stem, leaf, flower, fruit etc., is planting
Most of form for being combined into glycoside or carbon glycosyl with sugar exists in object, and also some exists in a free form.Flavonoid
The pharmaceutical value of object is very high, and the brittleness that can reduce blood vessel, reduces blood lipid and cholesterol at the permeability for improving blood vessel, can effectively prevent
Control the diseases such as hypertension, cerebral hemorrhage, coronary heart diseases and angina pectoris.Many flavones ingredients have cough-relieving, eliminating the phlegm, relieving asthma and antibacterial
Effect, while there is diseases and the free radical resisting oxidations such as protect liver, treatment acute, chronic hepatitis, cirrhosis.
Selenium is microelement necessary to human body determined by the United Nations's health organization, is glutathione in humans and animals body
The confactor of peroxidase (glutathione per-oxidase, GSHPX), GSHPX are widely present in viable organism
Liver, kidney, the heart, lung etc., seleno provide the essential group in its activated centre for cysteine for the enzyme, and GSHPX utilizes glutathione
So that virose peroxide is reduced to harmless hydroxy compounds, decompose peroxide, scavenging capacity free radical position is protected
Membrane structure and function are protected, molecular damage is repaired.Therefore, there is selenium element anti-oxidant, anticancer, anti-aging, enhancing body to exempt from
The multiple biological functions such as epidemic disease power.
The product of spirulina producer, the country is mainly based on spirulina powder at present, deep processing for spirulina and and its
The deep processed product that its functional raw material compounds (such as natural flavonoid compound and Se-enriched yeast extract) is less, purport of the present invention
Solving this technical problem.
Summary of the invention
It is an object of the present invention to solving the deficiencies in the prior art, selenium enriched Spirulina powder is provided, the instant powder and day
Right chromocor extract compounding, nutritive value is high, in good taste, has great healthy nutritive value.
The second object of the present invention is to provide the preparation method of selenium enriched Spirulina powder.
The technical solution adopted by the present invention to solve the technical problems is:
Selenium enriched Spirulina powder, the spirulina instant powder are formed by the Raw material processing of following parts by weight: spirulina extract 60-80
Part, 20-30 parts of buckwheat, 10-15 parts of wolfberry fruit extract, 2-6 parts of sophora bud standard sclerutin, 6-10 parts of Se-enriched yeast extract；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud rutin raw material drying to moisture content being lower than 8%, is crushed, 60-80 mesh is crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: hexamethylphosphoramide 15-
25%, dipotassium hydrogen phosphate 5-8%, ammonium sulfate 3-6%, surplus are water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 40-60 DEG C with the prepared extracting solution of step S2, is stirred
0.5-1h is mixed, 30-45min, layering are stood；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin.
The medicines and health protection value of flavone compound is very high, and the brittleness that can reduce blood vessel, reduces the permeability for improving blood vessel
Blood lipid and cholesterol can effectively prevent the diseases such as hypertension, cerebral hemorrhage, coronary heart diseases and angina pectoris.In addition, many flavones ingredients
Have the function of cough-relieving, eliminating the phlegm, relieving asthma and antibacterial, while there are the diseases such as protect liver, treatment acute, chronic hepatitis, cirrhosis, and anti-
Free-radical oxidation effect.
The method of common extracting flavonoids has the methods of alcohol extracting method, microwave loss mechanisms, supercritical extraction technique, membrane separation process at present,
But in the actual production process have step is cumbersome, energy consumption is high, yield is lower, solvent usage amount is big, solvent volatilization, extraction when
Between it is longer, can not cope with that flavones is heated easily to be decomposed, the weak disadvantage of stability.
The present invention with inorganic salts replace high polymer common organic matter double-aqueous phase system, suitable for polar substances extraction and
Purifying, split-phase is rapid, and the split-phase time is short, and extraction time is short, and extraction efficiency is high, and hexamethylphosphoramide is conducive to Huang in alkalinity
Letones are selectively allocated in upper phase in double-aqueous phase system, and extraction efficiency is high, and purification effect is good.In addition, at normal temperature into
Row, mild condition, product purity is higher, high income.
Preferably, the spirulina instant powder is formed by the Raw material processing of following parts by weight: 70 parts of spirulina extract, buckwheat
25 parts of flour, 12 parts of wolfberry fruit extract, 4 parts of sophora bud standard sclerutin, 8 parts of Se-enriched yeast extract；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud rutin raw material drying to moisture content being lower than 8%, is crushed, 60 meshes are crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: hexamethylphosphoramide
20%, dipotassium hydrogen phosphate 6%, ammonium sulfate 5%, surplus is water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 50 DEG C with the prepared extracting solution of step S2, is stirred
0.6h stands 45min, layering；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin.
Preferably, the mass ratio of raw material powder and the double-aqueous phase system is 1:10-1:15 in the step S3.
Preferably, the spirulina extract the preparation method is as follows:
S1: by the spirulina of fresh picking, after cleaning up, organization protection's liquid is infiltrated, freezes 1-3h preservation, the organization protection
The weight percent of liquid forms are as follows: the oxidized dextran of 2.0-5.0%, antioxidant 1.0-2.0%, bacteriostatic agent 0.5-0.8%；
Oxidized dextran is a kind of macromolecule glucose polymer that natural glucan obtains after sodium periodate oxidation, contains more aldehyde
Base has good stable cross-linking properties, and biocompatibility is fabulous, with other fibre fractionations can be fabulous it is compatible.The application
Inventor practice in find, multiple aldehyde radicals that oxidized dextran contains can be with high reaction activity exposed in spirulina crude fibre
Group carry out cross-linking reaction, play the role of intermolecular bridge formation dispersing agent, improve the stability and enzymatic hydrolysis of subsequent enzymatic hydrolysis stoste
Effect improves the quality of product.
S2: enzymatic hydrolysis stoste is purchased: by the spirulina of step S1 freezing in 60-70 DEG C of hot water, being sufficiently stirred mixed
Even to form stable enzymatic hydrolysis stoste, enzymatic hydrolysis stoste temperature is adjusted to 40-50 DEG C, and pH is adjusted to 6.5-7.2；Utilize the method for freeze thawing
Broken wall；
S3: the complex enzyme zymohydrolysis 45-60min of spirulina raw material weight in wet base 2-5% is added into the enzymatic hydrolysis stoste of step S2, digested
Maintain pH constant in journey, complex enzyme is the mixture of bromelain or papain and neutral cellulase, and enzymatic hydrolysis is completed
Enzyme deactivation, centrifugation obtain centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 3000-4500Da.
Preferably, the specific rotatory power of the oxidized dextran is 180-185, average molecular weight 4100-4200, oxidizability
For 130-135%, the antioxidant is reduced glutathione or ascorbic acid, and the bacteriostatic agent is bee glue powder, and tissue is protected
The synergistic effect for protecting liquid avoids enzymolysis process enzymolysis liquid rotten, and breeds bacterium；
Preferably, the step S3 complex enzyme is by papain or bromelain and neutral cellulase 6-8 in mass ratio:
The enzyme activity of 1 composition, complex enzyme is not less than 2000U/g.
Preferably, the Se-enriched yeast extract the preparation method is as follows:
S1: being collected by centrifugation the Se-enriched yeast that fermentation obtains, and infiltrates organization protection's liquid, freezes 1-3h preservation, organization protection's liquid
Weight percent composition are as follows: the oxidized dextran of 2.0-5.0%, antioxidant 1.0-2.0%, bacteriostatic agent 0.5-0.8%；
S2: enzymatic hydrolysis stoste is purchased: by the Se-enriched yeast of step S1 freezing in 70-80 DEG C of hot water, mixing is sufficiently stirred,
Stable enzymatic hydrolysis stoste is formed after homogeneous, enzymatic hydrolysis stoste temperature is adjusted to 45-55 DEG C, and pH is adjusted to 6.5-7.5；
S3: the complex enzyme zymohydrolysis 45-60min of Se-enriched yeast raw material weight in wet base 2-5%, enzymatic hydrolysis are added into the enzymatic hydrolysis stoste of step S2
Maintain pH constant in the process, complex enzyme is the mixture of bromelain or papain and 1,4 beta-glucanase, and enzymatic hydrolysis is completed
Enzyme deactivation, centrifugation obtain centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 4000-6000Da.
Preferably, in the step S3 complex enzyme by bromelain or papain and 1,4 beta-glucanase in mass ratio
The enzyme activity of 8-10:1 composition, complex enzyme is not less than 2500U/g.
Preferably, the weight percent composition of organization protection's liquid are as follows: 2.0% oxidized dextran, antioxidant
1.5%, bacteriostatic agent 0.6%.
The preparation method of selenium enriched Spirulina powder, it is described the preparation method is as follows: by the spirulina extract of formula ratio, buckwheat
Powder, wolfberry fruit extract, sophora bud standard sclerutin and Se-enriched yeast extract are uniformly mixed, and cross 80-100 mesh, Vacuum Package.
The beneficial effects of the present invention are: spirulina and sophora bud rutin are carried out deep processing by the present invention, spiral is made respectively
Algae extract, Se-enriched yeast extract and sophora bud standard sclerutin, wherein flavones content is high in sophora bud rutin, then with other components
Spirulina instant powder is made in compounding, and nutritive value is high, in good taste, has great healthy nutritive value.
In addition, the present invention replaces the common organic matter double-aqueous phase system of high polymer with inorganic salts, suitable for polar substances
It extracts and purifies, split-phase is rapid, and the split-phase time is short, and extraction time is short, and extraction efficiency is high, and hexamethylphosphoramide has in alkalinity
Upper phase is selectively allocated in double-aqueous phase system conducive to Flavonoid substances, extraction efficiency is high, and purification effect is good.In addition, normal
Temperature is lower to be carried out, and mild condition, product purity is higher, high income.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention will be further explained in detail.
Embodiment 1:
Selenium enriched Spirulina powder, the spirulina instant powder are formed by the Raw material processing of following parts by weight: 60 parts of spirulina extract,
20 parts of buckwheat, 10 parts of wolfberry fruit extract, 2 parts of sophora bud standard sclerutin, 6 parts of Se-enriched yeast extract；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud rutin raw material drying to moisture content being lower than 8%, is crushed, 60 meshes are crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: hexamethylphosphoramide
15%, dipotassium hydrogen phosphate 5%, ammonium sulfate 3%, surplus is water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 40 DEG C with the prepared extracting solution of step S2, is stirred
1h stands 45min, layering；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin；
The mass ratio of raw material powder and the double-aqueous phase system is 1:10 in the step S3；
The spirulina extract the preparation method is as follows:
S1: by the spirulina of fresh picking, after cleaning up, organization protection's liquid is infiltrated, freezes 1h preservation, organization protection's liquid
Weight percent composition are as follows: 2.0% oxidized dextran, antioxidant 1.0%, bacteriostatic agent 0.5%；
S2: enzymatic hydrolysis stoste is purchased: by the spirulina of step S1 freezing in 60 DEG C of hot water, mixing is sufficiently stirred and is formed surely
Fixed enzymatic hydrolysis stoste, enzymatic hydrolysis stoste temperature are adjusted to 40 DEG C, and pH is adjusted to 6.5；Utilize the method broken wall of freeze thawing；
S3: the complex enzyme zymohydrolysis 45min of spirulina raw material weight in wet base 2% is added into the enzymatic hydrolysis stoste of step S2, is tieed up in enzymolysis process
Hold that pH is constant, complex enzyme is the mixture of bromelain and neutral cellulase, and enzymatic hydrolysis completes enzyme deactivation, and centrifugation is centrifuged
Clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 3000Da.
The specific rotatory power of the oxidized dextran is 180-185, average molecular weight 4100-4200, oxidizability 130-
135%, the antioxidant is reduced glutathione or ascorbic acid, and the bacteriostatic agent is bee glue powder, organization protection's liquid
Synergistic effect avoids enzymolysis process enzymolysis liquid rotten, and breeds bacterium；The step S3 complex enzyme is by papain or spinach
Trailing plants protease and neutral cellulase 6:1 in mass ratio are formed, and the enzyme activity of complex enzyme is not less than 2000U/g.
The Se-enriched yeast extract the preparation method is as follows:
S1: being collected by centrifugation the Se-enriched yeast that fermentation obtains, and infiltrates organization protection's liquid, freezes 1h preservation, organization protection's liquid
Weight percent composition are as follows: 2.0% oxidized dextran, antioxidant 1.0%, bacteriostatic agent 0.5%；
S2: enzymatic hydrolysis stoste is purchased: by the Se-enriched yeast of step S1 freezing in 70 DEG C of hot water, mixing is sufficiently stirred,
Stable enzymatic hydrolysis stoste is formed after matter, enzymatic hydrolysis stoste temperature is adjusted to 45 DEG C, and pH is adjusted to 6.5；
S3: the complex enzyme zymohydrolysis 45-60min of Se-enriched yeast raw material weight in wet base 2% is added into the enzymatic hydrolysis stoste of step S2, digested
Maintain pH constant in journey, complex enzyme is the mixture of papain and 1,4 beta-glucanase, and enzymatic hydrolysis completes enzyme deactivation, is centrifuged, and is obtained
Centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 4000-6000Da.
Complex enzyme is by bromelain or papain and 1,4 beta-glucanase 8:1 group in mass ratio in the step S3
At the enzyme activity of complex enzyme is not less than 2500U/g.
By the spirulina extract of formula ratio, buckwheat, wolfberry fruit extract, sophora bud standard sclerutin and Se-enriched yeast extract
It is uniformly mixed, crosses 80 meshes, Vacuum Package.
Embodiment 2
Selenium enriched Spirulina powder, the spirulina instant powder are formed by the Raw material processing of following parts by weight: 80 parts of spirulina extract,
30 parts of buckwheat, 15 parts of wolfberry fruit extract, 6 parts of sophora bud standard sclerutin, 10 parts of Se-enriched yeast extract；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud rutin raw material drying to moisture content being lower than 8%, is crushed, 80 meshes are crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: hexamethylphosphoramide
25%, dipotassium hydrogen phosphate 8%, ammonium sulfate 6%, surplus is water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 60 DEG C with the prepared extracting solution of step S2, is stirred
0.5h stands 30min, layering；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin.
The mass ratio of raw material powder and the double-aqueous phase system is 1:15 in the step S3；
The spirulina extract the preparation method is as follows:
S1: by the spirulina of fresh picking, after cleaning up, organization protection's liquid is infiltrated, freezes 3h preservation, organization protection's liquid
Weight percent composition are as follows: 5.0% oxidized dextran, antioxidant 2.0%, bacteriostatic agent 0.8%；
S2: enzymatic hydrolysis stoste is purchased: by the spirulina of step S1 freezing in 70 DEG C of hot water, mixing is sufficiently stirred and is formed surely
Fixed enzymatic hydrolysis stoste, enzymatic hydrolysis stoste temperature are adjusted to 50 DEG C, and pH is adjusted to 7.2；Utilize the method broken wall of freeze thawing；
S3: the complex enzyme zymohydrolysis 60min of spirulina raw material weight in wet base 5% is added into the enzymatic hydrolysis stoste of step S2, is tieed up in enzymolysis process
Hold that pH is constant, complex enzyme is the mixture of papain and neutral cellulase, and enzymatic hydrolysis completes enzyme deactivation, and centrifugation is centrifuged
Clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 4500Da.
The specific rotatory power of the oxidized dextran is 180-185, average molecular weight 4100-4200, oxidizability 130-
135%, the antioxidant is reduced glutathione or ascorbic acid, and the bacteriostatic agent is bee glue powder, organization protection's liquid
Synergistic effect avoids enzymolysis process enzymolysis liquid rotten, and breeds bacterium；The step S3 complex enzyme is by papain or spinach
Trailing plants protease and neutral cellulase 8:1 in mass ratio are formed, and the enzyme activity of complex enzyme is not less than 2000U/g.
The Se-enriched yeast extract the preparation method is as follows:
S1: being collected by centrifugation the Se-enriched yeast that fermentation obtains, and infiltrates organization protection's liquid, freezes 3h preservation, organization protection's liquid
Weight percent composition are as follows: 5.0% oxidized dextran, antioxidant 2.0%, bacteriostatic agent 0.8%；
S2: enzymatic hydrolysis stoste is purchased: by the Se-enriched yeast of step S1 freezing in 80 DEG C of hot water, mixing is sufficiently stirred,
Stable enzymatic hydrolysis stoste is formed after matter, enzymatic hydrolysis stoste temperature is adjusted to 55 DEG C, and pH is adjusted to 7.5；
S3: the complex enzyme zymohydrolysis 60min of Se-enriched yeast raw material weight in wet base 5% is added into the enzymatic hydrolysis stoste of step S2, in enzymolysis process
Maintain pH constant, complex enzyme is the mixture of bromelain and 1,4 beta-glucanase, and enzymatic hydrolysis completes enzyme deactivation, and centrifugation is centrifuged
Clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 6000Da, and complex enzyme presses matter by bromelain or papain and 1,4 beta-glucanase in the step S3
Amount is formed than 10:1, and the enzyme activity of complex enzyme is not less than 2500U/g.
By the spirulina extract of formula ratio, buckwheat, wolfberry fruit extract, sophora bud standard sclerutin and Se-enriched yeast extract
It is uniformly mixed, crosses 80-100 mesh, Vacuum Package.
Embodiment 3
Selenium enriched Spirulina powder, the spirulina instant powder are formed by the Raw material processing of following parts by weight: 70 parts of spirulina extract,
25 parts of buckwheat, 12 parts of wolfberry fruit extract, 4 parts of sophora bud standard sclerutin, 8 parts of Se-enriched yeast extract；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud rutin raw material drying to moisture content being lower than 8%, is crushed, 60 meshes are crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: hexamethylphosphoramide
20%, dipotassium hydrogen phosphate 6%, ammonium sulfate 5%, surplus is water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 50 DEG C with the prepared extracting solution of step S2, is stirred
0.6h stands 45min, layering；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin, the Central Plains step S3 feed powder
The last mass ratio with the double-aqueous phase system is 1:12；
The spirulina extract the preparation method is as follows:
S1: by the spirulina of fresh picking, after cleaning up, organization protection's liquid is infiltrated, freezes 2h preservation, organization protection's liquid
Weight percent composition are as follows: 2.0% oxidized dextran, antioxidant 1.5%, bacteriostatic agent 0.6%；
S2: enzymatic hydrolysis stoste is purchased: by the spirulina of step S1 freezing in 65 DEG C of hot water, mixing is sufficiently stirred and is formed surely
Fixed enzymatic hydrolysis stoste, enzymatic hydrolysis stoste temperature are adjusted to 45 DEG C, and pH is adjusted to 7.0；Utilize the method broken wall of freeze thawing；
S3: the complex enzyme zymohydrolysis 50min of spirulina raw material weight in wet base 3% is added into the enzymatic hydrolysis stoste of step S2, is tieed up in enzymolysis process
Hold that pH is constant, complex enzyme is the mixture of bromelain or papain and neutral cellulase, and enzymatic hydrolysis completes enzyme deactivation, from
The heart obtains centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 3600Da.
The specific rotatory power of the oxidized dextran is 180-185, average molecular weight 4100-4200, oxidizability 130-
135%, the antioxidant is reduced glutathione or ascorbic acid, and the bacteriostatic agent is bee glue powder, organization protection's liquid
Synergistic effect avoids enzymolysis process enzymolysis liquid rotten, and breeds bacterium, and the step S3 complex enzyme is by papain in
Property cellulase 7:1 in mass ratio composition, the enzyme activity of complex enzyme is not less than 2000U/g.
The Se-enriched yeast extract the preparation method is as follows:
S1: being collected by centrifugation the Se-enriched yeast that fermentation obtains, and infiltrates organization protection's liquid, freezes 2h preservation, organization protection's liquid
Weight percent composition are as follows: 2.0% oxidized dextran, antioxidant 1.5%, bacteriostatic agent 0.6%；
S2: enzymatic hydrolysis stoste is purchased: by the Se-enriched yeast of step S1 freezing in 75 DEG C of hot water, mixing is sufficiently stirred,
Stable enzymatic hydrolysis stoste is formed after matter, enzymatic hydrolysis stoste temperature is adjusted to 50 DEG C, and pH is adjusted to 7.0；
S3: the complex enzyme zymohydrolysis 50min of Se-enriched yeast raw material weight in wet base 3% is added into the enzymatic hydrolysis stoste of step S2, in enzymolysis process
Maintain pH constant, complex enzyme is the mixture of bromelain or papain and 1,4 beta-glucanase, and enzymatic hydrolysis completes enzyme deactivation, from
The heart obtains centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 5000Da.
Complex enzyme is made of papain and 1,4 beta-glucanase 9:1 in mass ratio in the step S3, the enzyme of complex enzyme
It is living to be not less than 2500U/g.
By the spirulina extract of formula ratio, buckwheat, wolfberry fruit extract, sophora bud standard sclerutin and Se-enriched yeast extract
It is uniformly mixed, crosses 80-100 mesh, Vacuum Package.
Above-mentioned embodiment is only a preferred solution of the present invention, not the present invention is made in any form
Limitation, there are also other variations and modifications on the premise of not exceeding the technical scheme recorded in the claims.
Claims (10)
1. selenium enriched Spirulina powder, it is characterised in that: the selenium enriched Spirulina powder is formed by the Raw material processing of following parts by weight: spiral
60-80 parts of algae extract, 20-30 parts of buckwheat, 10-15 parts of wolfberry fruit extract, 2-6 parts of sophora bud standard sclerutin, Se-enriched yeast mentions
Take 6-10 parts of object；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud raw material drying to moisture content being lower than 8%, is crushed, 60-80 mesh is crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: hexamethylphosphoramide 15-
25%, dipotassium hydrogen phosphate 5-8%, ammonium sulfate 3-6%, surplus are water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 40-60 DEG C with the prepared extracting solution of step S2, is stirred
0.5-1h is mixed, 30-45min, layering are stood；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin.
2. selenium enriched Spirulina powder according to claim 1, it is characterised in that: the selenium enriched Spirulina powder is by following parts by weight
Raw material processing form: it is 70 parts of spirulina extract, 25 parts of buckwheat, 12 parts of wolfberry fruit extract, 4 parts of sophora bud standard sclerutin, rich
8 parts of selenium yeast extract；
The extracting method of the sophora bud standard sclerutin is as follows:
S1 raw material smashes: sophora bud raw material drying to moisture content being lower than 8%, is crushed, 60 meshes are crossed；
S2 aqueous two-phase extracting solution is prepared: the weight percent composition of the aqueous two-phase extracting solution is as follows: hexamethylphosphoramide
20%, dipotassium hydrogen phosphate 6%, ammonium sulfate 5%, surplus is water；
S3 is extracted: the smashed raw material powder of step S1 being mixed at 50 DEG C with the prepared extracting solution of step S2, is stirred
0.6h stands 45min, layering；
S4 separation: it takes upper organic phase to be concentrated, precipitates crystal as sophora bud standard sclerutin.
3. selenium enriched Spirulina powder according to claim 1 or 2, it is characterised in that: raw material powder and institute in the step S3
The mass ratio for stating double-aqueous phase system is 1:10-1:15.
4. selenium enriched Spirulina powder according to claim 1, it is characterised in that:
The spirulina extract the preparation method is as follows:
S1: by the spirulina of fresh picking, after cleaning up, organization protection's liquid is infiltrated, freezes 1-3h preservation, the organization protection
The weight percent of liquid forms are as follows: the oxidized dextran of 2.0-5.0%, antioxidant 1.0-2.0%, bacteriostatic agent 0.5-0.8%；
S2: enzymatic hydrolysis stoste is purchased: by the spirulina of step S1 freezing in 60-70 DEG C of hot water, mixing shape is sufficiently stirred
At stable enzymatic hydrolysis stoste, digests stoste temperature and adjust to 40-50 DEG C, pH is adjusted to 6.5-7.2；
S3: the complex enzyme zymohydrolysis 45-60min of spirulina raw material weight in wet base 2-5% is added into the enzymatic hydrolysis stoste of step S2, digested
Maintain pH constant in journey, complex enzyme is the mixture of bromelain or papain and neutral cellulase, and enzymatic hydrolysis is completed
Enzyme deactivation, centrifugation obtain centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 3000-4500Da.
5. selenium enriched Spirulina powder according to claim 4, it is characterised in that: the specific rotatory power of the oxidized dextran is
180-185, average molecular weight 4100-4200, oxidizability 130-135%, the antioxidant be reduced glutathione or
Person's ascorbic acid, the bacteriostatic agent are bee glue powder.
6. selenium enriched Spirulina powder according to claim 4, it is characterised in that: the step S3 complex enzyme is by papain
Or bromelain and neutral cellulase 6-8:1 in mass ratio are formed, the enzyme activity of complex enzyme is not less than 2000U/g.
7. selenium enriched Spirulina powder according to claim 1, it is characterised in that:
The Se-enriched yeast extract the preparation method is as follows:
S1: being collected by centrifugation the Se-enriched yeast that fermentation obtains, and infiltrates organization protection's liquid, freezes 1-3h preservation, organization protection's liquid
Weight percent composition are as follows: the oxidized dextran of 2.0-5.0%, antioxidant 1.0-2.0%, bacteriostatic agent 0.5-0.8%；
S2: enzymatic hydrolysis stoste is purchased: by the Se-enriched yeast of step S1 freezing in 70-80 DEG C of hot water, mixing is sufficiently stirred,
Stable enzymatic hydrolysis stoste is formed after homogeneous, enzymatic hydrolysis stoste temperature is adjusted to 45-55 DEG C, and pH is adjusted to 6.5-7.5；
S3: the complex enzyme zymohydrolysis 45-60min of Se-enriched yeast raw material weight in wet base 2-5%, enzymatic hydrolysis are added into the enzymatic hydrolysis stoste of step S2
Maintain pH constant in the process, complex enzyme is the mixture of bromelain or papain and 1,4 beta-glucanase, and enzymatic hydrolysis is completed
Enzyme deactivation, centrifugation obtain centrifugal clear liquid；
S4: the centrifugal clear liquid of step S3, using ultrafiltration membrane ultrafiltration, permeate is concentrated, is spray-dried, the ultrafiltration membrane
Molecular cut off is 4000-6000Da.
8. selenium enriched Spirulina powder according to claim 7, it is characterised in that: complex enzyme is by bromelain in the step S3
Enzyme or papain and 1,4 beta-glucanase 8-10:1 in mass ratio form, and the enzyme activity of complex enzyme is not less than 2500U/g.
9. the selenium enriched Spirulina powder according to claim 4 or 7, it is characterised in that: the weight percent of organization protection's liquid
Than composition are as follows: 2.0% oxidized dextran, antioxidant 1.5%, bacteriostatic agent 0.6%.
10. a kind of preparation method of selenium enriched Spirulina powder described in any one of claim 1-9, which is characterized in that the system
Preparation Method is as follows: the spirulina extract of formula ratio, buckwheat, wolfberry fruit extract, sophora bud standard sclerutin and Se-enriched yeast are mentioned
It takes object to be uniformly mixed, crosses 80-100 mesh, Vacuum Package.
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